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Rostov-na-Donu, Russia

Goroshinskaya I.G.,Rostov Oncological Research Institute | Tarnopol'skaya O.V.,Rostov Oncological Research Institute | Kachesova P.S.,Rostov Oncological Research Institute
Eksperimental'naya i Klinicheskaya Farmakologiya

The fluorescence intensity of acridine orange (AO) dye bound to living human leukocytes, rat leukocytes, and Pliss lymphosarcoma cells of rat has been studied by fluorescence microscopy. The cell suspension of each test was divided into two parts, to one of which the drug cisplatin was added to a final concentration of 0.1 μg/mL and the mixture was incubated for 20-60 min. Acridine orange was then added and the average intensity of red and green fluorescence of cells was determined. The brightness of AO in the green region of the spectrum was reduced approximately 3-fold (p = 0.001) in cells with cisplatin. In the red region of the fluorescence, differences in the brightness of cells with and without cisplatin were not observed. These data indicate that a decrease in the brightness of green fluorescence of AO can be a test for the presence of cisplatin in cells. © Folium Publishing House, 2014. Source

Kit O.I.,Rostov Oncological Research Institute | Frantsiyants E.M.,Rostov Oncological Research Institute | Kaplieva I.V.,Rostov Oncological Research Institute | Trepitaki L.K.,Rostov Oncological Research Institute | Evstratova O.F.,Rostov Oncological Research Institute
Bulletin of Experimental Biology and Medicine

A new method for reproduction of metastatic involvement of the liver in outbred albino rats is proposed. The use of the method rules out the effects of stress factors (surgical intervention, total anesthesia) on malignant tumor metastasizing, making it maximally similar to the natural process. The method allows visual evaluation of the time course of the primary tumor node growth. The metastatic involvement of the liver develops only by the most significant route, hematogenic. The method can be used for the development of experimental therapy for this condition. © 2014, Springer Science+Business Media New York. Source

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