Bielen A.,University of Zagreb |
Vladusic T.,University of Zagreb |
Kuharic N.,Rooseveltov trg |
Hudina S.,Rooseveltov trg |
And 3 more authors.
Periodicum Biologorum | Year: 2014
Background and Purpose: Te signal crayfsh Pacifastacus leniusculus (Dana, 1852) is one of the most successful invasive species of crayfsh in European freshwaters, an extremely diverse though endangered group of ecosystems. Te main goal of this study was to functionally characterize multixenobiotic resistance (MXR) mechanism defense activity in P. lenius-culus tissues for the frst time. MXR mechanism protects the cell from a wide variety of toxic compounds, and it is mediated by the transport activity of ATP-binding cassette (ABC) proteins. Materials and Methods: MXR transporter activity dye assay was performed by using fuorescent model substrate rhodamine B (RB) in combination with inhibitors of MXR efux pumps: MK571 and Verapamil, known to inhibit multidrug resistance-associated proteins (MRP) and P-glycoprotein (P-gp), respectively. In this assay, the increase in intracellular fuorescence of the substrate dye, indicates inhibition of MXR efux protein pumps. Te assay was performed in three diferent tissues (gills, hepatopancreas, tail muscle). Additionally, tissues were exposed to selected heavy metals – mercury (HgCl2) and zinc (ZnCl2), known to occur in open freshwaters as pollutants. Results: Optimal time for RB accumulation in gills and hepatopancreas was determined to be 30 minutes. RB efux in gills was inhibited by MK571 and in hepatopancreas by Verapamil, suggesting that multidrug resistance-associated proteins are dominant in gills of P. leniusculus, and P-glycoprotein in hepatopancreas. Finally, inhibitory efect of mercury (HgCl2: 10 and 20 µM) and zinc (ZnCl2: 5–20 µM) on multixenobiotic resistance mechanism activity in gills, and only mercury in hepatopancreas, was detected. Conclusions: Te results for the frst time demonstrate the presence of multixenobiotic resistance mechanism efux activity as an important tissue specifc defense mechanism in P. leniusculus and provide the basis for future molecular and toxicological studies of this invasive and adaptable species. © 2014, Croatian Society of Natural Sciences. All rights Reserved.
Stankovic V.M.,Croatian Natural History Museum |
Jach M.A.,Naturhistorisches Museum Wien |
Kucinic M.,Rooseveltov trg
Natura Croatica | Year: 2015
In this paper an annotated checklist of Croatian riffle beetles is presented. It is a result of a literature review, revision of museum collections, implementation of results from various environmental impact and baseline studies, as well as field investigations throughout Croatia from 2002-2013. Altogether 23 species in eight genera of Elmidae were identified. Five species are new for the Croatian fauna: Elmis rioloides (Kuwert 1890), Esolus angustatus (Müller 1821), E. pygmaeus (Müller 1806), Oulimnius tuberculatus (Müller 1806) and Stenelmis consobrina Dufour 1835. Furthermore, the occurrence of Elmis aenea (Müller 1806) in Croatia is confirmed. © 2015, Croatian Natural History Museum. All rights reserved.
Antonovic I.,Slavka Kolara 45 |
Brigic A.,Rooseveltov trg |
Bedek J.,Croatian Biospeleological Society |
Sedlar Z.,Marulicev trg
Natura Croatica | Year: 2015
This paper presents the distribution of the terrestrial isopod Hyloniscus adonis (Verhoeff, 1927) in Croatia. It was recorded for the first time at the edge of Dubravica peat bog and the adjacent forest. Additionally, it was collected at the edges of Đon močvar peat bog. Revision of the terrestrial isopod collection of the Croatian Biospeleological Society revealed that this species also occurs in central and eastern part of Croatia. Our results reveal that H. adonis is a hygrophilic species inhabiting various types of forests in the colline and montane belts. It prefers humid soils with thick litter and humus layers and most probably seldom leaves its shelter. H. adonis is extremely rare in open bog habitats, most likely due to the harsh environmental conditions (e.g. high soil humidity, low pH values) and can be considered as a tyrphoxenous species. Additionally, it was also found in the entrance zones of caves, most likely due to the favourable microclimatic conditions. It seems to be a temporary cave dweller and can be classified as a trogloxenous species. © 2015, Croatian Natural History Museum. All rights reserved.