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Albulescu R.,Victor Babes National Institute of Pathology | Albulescu R.,Romanian National Institute for Chemical Pharmaceutical Research and Development | Tanase C.,Victor Babes National Institute of Pathology | Codrici E.,Victor Babes National Institute of Pathology | And 5 more authors.
Journal of Cellular and Molecular Medicine | Year: 2015

Telocytes (TCs) are interstitial cells that are present in numerous organs, including the heart interstitial space and cardiac stem cell niche. TCs are completely different from fibroblasts. TCs release extracellular vesicles that may interact with cardiac stem cells (CSCs) via paracrine effects. Data on the secretory profile of TCs and the bidirectional shuttle vesicular signalling mechanism between TCs and CSCs are scarce. We aimed to characterize and understand the in vitro effect of the TC secretome on CSC fate. Therefore, we studied the protein secretory profile using supernatants from mouse cultured cardiac TCs. We also performed a comparative secretome analysis using supernatants from rat cultured cardiac TCs, a pure CSC line and TCs-CSCs in co-culture using (i) high-sensitivity on-chip electrophoresis, (ii) surface-enhanced laser desorption/ionization time-of-flight mass spectrometry and (iii) multiplex analysis by Luminex-xMAP. We identified several highly expressed molecules in the mouse cardiac TC secretory profile: interleukin (IL)-6, VEGF, macrophage inflammatory protein 1α (MIP-1α), MIP-2 and MCP-1, which are also present in the proteome of rat cardiac TCs. In addition, rat cardiac TCs secrete a slightly greater number of cytokines, IL-2, IL-10, IL-13 and some chemokines like, GRO-KC. We found that VEGF, IL-6 and some chemokines (all stimulated by IL-6 signalling) are secreted by cardiac TCs and overexpressed in co-cultures with CSCs. The expression levels of MIP-2 and MIP-1α increased twofold and fourfold, respectively, when TCs were co-cultured with CSCs, while the expression of IL-2 did not significantly differ between TCs and CSCs in mono culture and significantly decreased (twofold) in the co-culture system. These data suggest that the TC secretome plays a modulatory role in stem cell proliferation and differentiation. © 2015 The Authors.

Nichita C.,University of Bucharest | Nichita C.,Romanian National Institute for Chemical Pharmaceutical Research and Development | Stamatin I.,University of Bucharest
Digest Journal of Nanomaterials and Biostructures | Year: 2012

This study reveal the relationship between SWCNTs (SWCNTs, carboxylated respective, hydroxylated) with a series of flavonoids (quercetin, rutin) to design new bionanohybrids with higher antioxidant capacity. The chemiluminescence assay proved that the carbon nanotubes have antioxidant activity as electron donor or as proton donor (SWCNTs -hydroxylated and carboxylated) in the redox transition reaction with free radicals. The bi-ohybrids "flavonoids carbon nanotubes" shown a high antioxidant activity, evaluated by the amplification factor, ranging from 3 up to 4 times higher than single flavonoids. These results open the perspective to design functional carriers based on carbon nanotubes to transport low soluble antioxidants to specific target with improved scavenging rate against the free radicals (reactive oxygen species).

Vamanu E.,University of Agronomical Sciences and Veterinary Medicine | Nita S.,Romanian National Institute for Chemical Pharmaceutical Research and Development
BioMed Research International | Year: 2013

Boletus edulis is a wild edible mushroom habitually consumed by rural populations. Ethanolic and methanolic extracts was obtained in cold and hot water from dried fruit bodies. The antioxidant activity of freeze-dried extracts from B. edulis were investigated using free radicals scavenging activity, reducing power, metal chelating effect, inhibition of lipid peroxidation, and the identification of antioxidant compounds. The levels of different compounds with antioxidant properties were higher in alcoholic extracts compared with aqueous extracts. Rosmarinic acid was the major phenolic compound, it being identified in a concentration between 7±0.23 and 56±0.15 mg/100 g extract. A positive correlation between the content of total phenols, flavonoids, anthocyanins, and tocopherols, and the antioxidant capacity of the extracts was determined. The results showed that the ethanolic extract of Romanian wild mushroom B. edulis represents a natural source of functional compounds. © 2013 Emanuel Vamanu and Sultana Nita.

Cioates Negut C.,Romanian National Institute for Chemical Pharmaceutical Research and Development | Cioates Negut C.,Polytechnic University of Bucharest | Stefan-Van Staden R.-I.,Romanian National Institute for Research for Electrochemistry and Condensed Matter | Moldoveanu I.,Romanian National Institute for Research for Electrochemistry and Condensed Matter | And 2 more authors.
Electrochemistry Communications | Year: 2015

Six oleamides: oleoylethanolamide, (Z)-N-[(1S)-2-hydroxy-1-(phenylmethyl)ethyl]-9octadecenamide, N-phenethyloleamide, N-[2-(4-methoxyphenyl)ethyl]oleamide, N-[1]naphthyloleamide, N-cyclohexyloleamide were synthesized and used as modifiers for the design of new stochastic microsensors based on graphite paste. Carcinoembryonic antigen (CEA) was used as a model analyte to prove the stochastic behavior of the microsensors. CEA was determined directly from whole blood samples with recoveries higher than 94.00%, favorizing pattern recognition of CEA in whole blood samples. The most sensitive stochastic microsensor was the one based on the physical immobilization of N-[2-(4-methoxyphenyl)ethyl]oleamide in graphite paste. © 2014 Elsevier B.V. All rights reserved.

Iulian O.,Polytechnic University of Bucharest | Stefaniu A.,Romanian National Institute for Chemical Pharmaceutical Research and Development
Journal of Solution Chemistry | Year: 2013

The physico-chemical property data for l-glutamic acid in aqueous NaCl solutions were obtained at 0, 0.5, 1.0 and 2.0 mol·kg-1 NaCl and amino acid molalities from 0 to 0.0669 mol·kg-1 between 293.15 and 323.15 K. The influence of NaCl addition on the volumetric and viscometric properties of the amino acid has been studied. From the experimental densities, the apparent molar volumes and transfer volumes of l-glutamic acid in aqueous electrolyte solutions have been calculated. The viscosity data in the studied domain of amino acid molalities have led to the evaluation of the Falkenhagen and Jones-Dole viscosity coefficients of l-glutamic acid in NaCl aqueous solutions. The results have been discussed in terms of interactions occurring in the systems. © 2013 Springer Science+Business Media New York.

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