Akhter S.,Jamia Hamdard University |
Ahmad J.,Jamia Hamdard University |
Ahmad M.Z.,Najran University |
Beg S.,Roland Institute of Pharmaceutical science |
Ahmad F.J.,Jamia Hamdard University
Expert Opinion on Drug Delivery | Year: 2015
Introduction: Psoriasis is a T-cell mediated autoimmune inflammatory skin disease recognized by skin surface inflammation, epidermal proliferation, hyperkeratosis, angiogenesis and anomalous keratinization. Currently, various pharmacotherapies are available for it; however, pharmacotherapy based on conventional formulations can provide therapeutic benefits only to a limited extent. Recent advancement in nanotechnology-based nanomedicines has led to the possibility of improving the efficacy and safety of pharmacotherapeutic agents for psoriasis.Areas covered: This review covers the brief pathophysiology of psoriasis, available medications and its associated challenges in treatment. Collective accounts of various drugs acting on different molecular targets of psoriasis and the role of nanomedicines in their effective targeting are discussed. Moreover, newer approaches in psoriatic therapy such as combination drug targeting and physical techniques of topical permeation enhancement along with nanomedicines are also discussed.Expert opinion: Novel nanomedicines (such as liposomes, polymeric nanoparticles, etc.) have shown their potential in improving therapeutic benefits of antipsoriatic drugs by increasing their therapeutic efficacy with minimal toxicity. Nevertheless, while the results on animal models using nanomedicine-based drug targeting of psoriasis via different route seem promising, lack of sufficient evidence in a clinical setup is a constraint and more clinical studies on the efficacy and safety of nanomedicines in psoriasis therapy are required. © 2014 Informa UK, Ltd.
Patro V.J.,Roland Institute of Pharmaceutical science |
Dinda S.C.,Berhampur University
Pharmacognosy Reviews | Year: 2012
Plants of genus Leucas (Lamiaceae) are widely distributed throughout Asia, Africa, and India. The plant is used in traditional medicine to cure many diseases such as cough, cold, diarrhea, and inflammatory skin disorder. A variety of phytoconstituents have been isolated from the Leucas species, which include lignans, flavonoids, coumarins, steroids, terpenes, fatty acids, and aliphatic long-chain compounds. Anti-inflammatory, analgesic, anti-diarrheal, antimicrobial, antioxidant, and insecticidal activities have been reported in the extracts of these plants and their phytoconstituents. An overview of the ethnobotanical, phytochemical, and pharmacological investigations on the Leucas species is presented in this review.
Mathrusri Annapurna M.,Gandhi Institute of Technology and Management |
Nanda B.,Roland Institute of Pharmaceutical science
Journal of Pharmacy and Nutrition Sciences | Year: 2011
Abstract: A simple precise, accurate RP-HPLC method has been developed and validated for analysis of Zolmitriptan (ZLM). The separation and quantization were achieved on a 250 mm reversed phase column with a hydrophilic linkage between silica particles and hydrophobic alkyl chains. The mobile phase was constituted (flow rate 0.8 ml min-1) of eluant A (CH3OH) and eluant B (aqueous tetra butyl ammonium hydrogen sulphate) (pH 3.4; 10 mM) using isocratic elution with UV detection at 224 nm. The method showed good linearity for ZMT in the 1-100 μg mL-1 range with regression equation 15576x ± 99401 and correlation coefficient 0.999 respectively. The limit of quantitation (LOQ) and limit of detection (LOD) were found to be 0.8134 and 0.2687 μg mL-1 respectively. Finally the applicability of the method was validated according to ICH guidelines and can be applicable for the analysis of commercial dosage forms.
Annapurna M.M.,Gandhi Institute of Technology and Management |
Mohapatro C.,Roland Institute of Pharmaceutical science |
Narendra A.,Roland Institute of Pharmaceutical science
Journal of Pharmaceutical Analysis | Year: 2012
A stability-indicating high-performance liquid chromatographic method was developed and validated for the determination of Letrozole in tablet dosage forms. Reversed-phase chromatography was performed on Shimadzu Model LC-Class-Vp with Lichrocart/Lichrosphere 100 C-18 (250 mm×4.6 mm, 5 μm particle size) column with methanol: tetra butyl ammonium hydrogen sulfate (80:20V/V) as mobile phase at a flow rate of 1 mL/min with UV detection at 240 nm. Linearity was observed in the concentration range of 0.5-150 μg/mL (R 2=0.9998) with regression equation y=102582x43185. The limit of quantitation (LOQ) and limit of detection (LOD) were found to be 0.043 and 0.012 μg/mL respectively. The forced degradation studies were performed by using HCl, NaOH, H2O2, thermal and UV radiation. Letrozole is more sensitive towards alkaline conditions and very much resistant towards acidic, oxidative and photolytic degradations. The method was validated as per ICH guidelines. The RSD for intra-day (0.78-0.97) and inter-day (0.86-0.96) precision were found to be lesser than 1%. The percentage recovery was in good agreement with the labeled amount in the pharmaceutical formulations and the method is simple, specific, precise and accurate for the determination of Letrozole in pharmaceutical formulations. © 2012 Xi'an Jiaotong University.
Sahu K.,Roland Institute of Pharmaceutical science |
Bisht Dr. S.S.,Roland Institute of Pharmaceutical science
International Journal of Pharma and Bio Sciences | Year: 2013
Parkinson's disease PARK1, caused by mutations in the SNCA gene, which codes for the protein alpha-synuclein. SNCA gene is analysed by different NCBI tools and bioinformatics softwares (ORF, Map Viewer, e-PCR, Vec screen, Genscan, BLAST, FASTA, MSA, ClustalW, bioedit software and phylodraw software). Then protein information and protein structures are analysed by different proteomics tools and different softwares (Protparam, Protscale, GOR, SOPMA, SignalP, NetNGly, NetOGly, NetAcet, NetPhos, sulfinator, SOSUI, bioedit software, SPDBV software, RASMOL 3D analysis software). Homology modelling of the protein is done using SPDBV (SWISS PDB VIEWER). Active site analysis is done through Q-site finder method and the active site amino acids are noted. Standard available market drugs targeting the protein were identified as Carbidopa, Dopamine, Levodopa, Memantine, and Apomorphine. 16 similar molecules for these standard molecules were modelled using Argus Lab software. A database is created with all these molecules in Vega ZZ software. Virtual screening of these drugs is done through protein-database docking method. QSAR analysis is done through Hyperchem software. UV & IR transitions are done through CAche software.
Chowdhury B.,Roland Institute of Pharmaceutical science |
Bhattamisra S.K.,Roland Institute of Pharmaceutical science |
Das M.C.,NRI Medical College
Indian Journal of Pharmacology | Year: 2013
Objectives: The aim of the present study was to evaluate the anti-convulsant potential of aqueous and ethanol e xtract of Glycyrrhiza glabra (AEGG and EEGG) and its action on markers of oxidant stress in albino rats. Materials and Methods: The aqueous and ethanol extract of Glycyrrhiza glabra was tested at three doses viz. 100, 200, and 400 mg/kg i.p. for its anti-convulsant activity using pentylenetetrazole (PTZ)-induced seizure in rat. The effect of EEGG (400 mg/kg, i.p.) on oxidative stress markers like malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT) of rat brain tissue homogenate was tested. Results: The onset of seizure was delayed (P < 0.01) by all the three doses of EEGG, but the duration of convulsion was reduced (P < 0.01) only in higher dose level (200 and 400 mg/kg), whereas AEGG up to 400 mg/kg did not alter any of the parameters significantly. Biochemical analysis of rat brain tissue revealed that MDA was increased (P < 0.01), whereas SOD and CAT were decreased (P < 0.01) in PTZ-induced seizure rat, whereas pre-treatment with EEGG (400 mg/kg) decreased (P < 0.01) the MDA and increased (P < 0.01) both SOD and CAT, indicating attenuation of lipid peroxidation due to increase in antioxidant enzymes. Conclusion: The results demonstrated that EEGG poses anti-convulsant potential and ameliorates ROS induced neuronal damage in PTZ-induced seizure.
Rao M.E.B.,Roland Institute of Pharmaceutical science |
Rajurkar V.G.,Roland Institute of Pharmaceutical science
Asian Journal of Chemistry | Year: 2011
As oxadiazole have proven to be good antimicrobial agents, Antitubercular, analgesic (peripheral and central) and antiinflammatory agents. A new series of oxadiazole derivatives were synthesized and characterized by 1H NMR, IR, GCMS sophisticated analytical instruments and were evaluated for their antimicrobial activity, antitubercular activity, toxicity as per standard guidelines, analgesic (peripheral and central) and antiinflammatory activity. Out of several derivatives synthesized a few of N-phenyl substituted 2-(5-(pyridine-4- yl)-1,3,4-oxadiazole-2-yl thio)acetamide explores good antimicrobial activity by using Cup-Plate method, antitubercular activity by middle brook 7H9 agar medium against H37Rv, analgesic activity by Writhing and Tail immersion method and antiinflammatory by rat paw edema method.
Bisht S.P.S.,Roland Institute of Pharmaceutical science |
Panda A.K.,Roland Institute of Pharmaceutical science
International Journal of Pharma and Bio Sciences | Year: 2011
Thermophilic lipase producing bacteria have been isolated and characterized from an Indian hot spring, isolates named as AK-P1, AK-P2 and AK-P3 respectively. The strain AK-P2 was first of its kind with reference to its lipase producing potential. The phylogenetic analysis of these strains using 16S rDNA sequence data revealed that the strain AK-P1 had highest homology (100%) with Acinetobacter sp. 01B0;, AK-P2 had highest homology (99.9%) with Brevibacillus borstelensis; AR9 and AK-P3 have shown 99.2 % similarities with Porphyrobacter cryptus (T); ALC-2. Maximal lipase production by AK-P2 was observed at the end of the stationary phase (36 h). The optimum temperature and pH for this crude enzyme activity was 60 oC and 10.0 respectively. Enzyme extracted from AK-P2 exhibited a Km 0.052mM and Vmax 0.645 μM/min/ml with p-nitrophenyl laurate as a substrate.
Kota S.K.,Medwin Hospital |
Kota S.K.,Central Security Hospital |
Jammula S.,Roland Institute of Pharmaceutical science |
Panda S.,Jawaharlal Institute of Postgraduate Medical Education & Research |
Modi K.D.,Medwin Hospital
Diabetes Technology and Therapeutics | Year: 2011
Diabetic cardiomyopathy is a distinct entity in humans. It leads to ventricular dysfunction independent of and additive to coronary artery disease and hypertension. Clinical and experimental studies have pointed to the role of metabolic derangements in the development of diabetic cardiomyopathy. Altered insulin signaling in diabetes leads to decreased myocyte glucose uptake and utilization, associated with an increased concentration of free fatty acids. This results in decreased glucose oxidation and increased fatty acid oxidation. Fatty acids increase mitochondrial oxygen consumption for ATP production and stimulate the uncoupling proteins in mitochondria. These proteins decrease the mitochondrial protein gradient, leading to fall in ATP production. The resultant defect in myocardial energy production impairs myocyte contraction and diastolic function. This is the hallmark of diabetic cardiomyopathy at earlier stages. In later stages diabetes impairs the myocyte ischemic defense mechanism, leading to increased cardiovascular morbidity and mortality. Other factors contributing toward causation of diabetic cardiomyopathy are collagen accumulation leading to reduced myocardial compliance, accumulation of advanced glycation end product-modified extracellular matrix proteins with subsequent inelasticity of vessel walls and myocytes, abnormal myocardial calcium handling leading to altered mechanics, endothelial dysfunction, cardiac autonomic neuropathy, and impairment of ischemic preconditioning. Trimetazidine acts a metabolic switch, favoring glucose over free fatty acids as the substrate for metabolism in cardiac myocytes. © 2011 Mary Ann Liebert, Inc.
Srinivasa Rao K.,Roland Institute of Pharmaceutical science |
Chaudhury P.K.,Roland Institute of Pharmaceutical science |
Pradhan A.,Roland Institute of Pharmaceutical science
Food and Chemical Toxicology | Year: 2010
The aim of this study was to assess the in vitro potential of chloroform extract of Chromolaena odorata leaves. The DPPH activity of the extract (0.1-5 mg/ml) was increased in a dose dependent manner, which was found in the range of 23.48-91.61% as compared to ascorbic acid (33.69-94.10%). The IC50 values of chloroform extract in DPPH radical, hydroxyl radical, nitric oxide, ABTS radical were obtained to be 0.31, 0.43, 0.28 and 1.32 mg/ml, respectively. However, the IC50 values for the standard ascorbic acid were noted to be 0.24, 0.41, 0.23 and 1 mg/ml, respectively. Measurement of total phenolic content of the chloroform extract of C. odorata was achieved using Folin-Ciocalteau reagent containing 242.2 mg/g of phenolic content, which was found significantly higher when compared to reference standard gallic acid. The results obtained in this study clearly indicate that C. odorata has a significant potential to use as a natural anti-oxidant agent. © 2009 Elsevier Ltd. All rights reserved.