The Rockefeller University is an American private university located in New York City in the United States, offering postgraduate and postdoctoral education. It conducts research mainly in biological science and medical science, and has produced or attracted many Nobel laureates. It has the highest number of Nobel Prizes in relation to personnel involved in research in the world. The Rockefeller University is located on the Upper East Side of Manhattan, between 63rd and 68th Streets along York Avenue.Marc Tessier-Lavigne—previously executive vice president of research and chief scientific officer at Genentech—became the university's tenth president on March 16, 2011.The Rockefeller University Press publishes the Journal of Experimental Medicine, the Journal of Cell Biology, and The Journal of General Physiology. Wikipedia.
Marraffini L.A.,Rockefeller University
Prokaryotic organisms are threatened by a large array of viruses and have developed numerous defence strategies. Among these, only clustered, regularly interspaced short palindromic repeat (CRISPR)-Cas systems provide adaptive immunity against foreign elements. Upon viral injection, a small sequence of the viral genome, known as a spacer, is integrated into the CRISPR locus to immunize the host cell. Spacers are transcribed into small RNA guides that direct the cleavage of the viral DNA by Cas nucleases. Immunization through spacer acquisition enables a unique form of evolution whereby a population not only rapidly acquires resistance to its predators but also passes this resistance mechanism vertically to its progeny. © 2015 Macmillan Publishers Limited. All rights reserved. Source
Steinman R.M.,Rockefeller University
Annual Review of Immunology
A properly functioning adaptive immune system signifies the best features of life. It is diverse beyond compare, tolerant without fail, and capable of behaving appropriately with a myriad of infections and other challenges. Dendritic cells are required to explain how this remarkable system is energized and directed. I frame this article in terms of the major decisions that my colleagues and I have made in dendritic cell science and some of the guiding themes at the time the decisions were made. As a result of progress worldwide, there is now evidence of a central role for dendritic cells in initiating antigen-specific immunity and tolerance. The in vivo distribution and development of a previously unrecognized white cell lineage is better understood, as is the importance of dendritic cell maturation to link innate and adaptive immunity in response to many stimuli. Our current focus is on antigen uptake receptors on dendritic cells. These receptors enable experiments involving selective targeting of antigens in situ and new approaches to vaccine design in preclinical and clinical systems. © 2012 by Annual Reviews. All rights reserved. Source
Chait B.T.,Rockefeller University
Annual Review of Biochemistry
Mass spectrometry (MS) is rapidly becoming an essential tool for biologists and biochemists in their efforts to throw light on molecular mechanisms within cellular systems. Used in unison with genome sequence data, MS has developed into the method of choice for identifying proteins, elucidating their posttranslational modifications, and reading out their functional interactions. Variations of the method have even begun to enable accurate mass determination of intact protein complexes, allowing for direct determination of subunit stoichiometry and the interactions between the subunits. Advances in mass spectrometric technologies have also led to great improvements in our ability to probe and define many of the other key molecular players in cells, including the all important lipid components. We provide here some perspectives on the explosion of applications of MS to protein science, systems biology, proteomics, lipidomics, and cell biology in general. © 2011 by Annual Reviews. All rights reserved. Source
Rockefeller University | Date: 2015-11-13
Rockefeller University | Date: 2015-03-20
Methods for rapidly obtaining a nanoscale apolipoprotein bound phospholipid bilayer (NABB) associated with at least one membrane protein are provided. Also disclosed are methods for rapidly obtaining a NABB not associated with membrane proteins. Immunogenic compositions comprising NABBs with native conformational epitopes are also provided along with their methods of use.