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Sibi G.,Robust Materials Technology Pvt. Ltd | Shukla A.,Robust Materials Technology Pvt. Ltd | Dhananjaya K.,Robust Materials Technology Pvt. Ltd | Ravikumar K.R.,Robust Materials Technology Pvt. Ltd | Mallesha H.,Robust Herbals Pvt. Ltd
Journal of Applied Pharmaceutical Science | Year: 2013

Foodborne diseases remain substantial and safety of food is an important health, social and economical issue. Food borne illnesses caused by microbial contamination raises concerns to find alternate sources which are safe to human and environmental health. This study was investigated to determine the antibacterial activity of broccoli (Brassica oleracea L. var. italica). Various solvent extracts of broccoli were prepared and analyzed for their phytoconstituents. A total of six food borne bacteria viz., Bacillus cereus ATCC 10876, Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 8739, Salmonella typhimurium MTCC 3224 and Shigella flexneri ATCC 12022 were tested against the broccoli extracts. Minimum inhibitory concentration (MIC) values of 10 - 320 μg ml-1 were recorded against most of the pathogens with acetone and methanol as the potential extracts. B. subtilis ATCC 6633 (15.4 mm) and Bacillus cereus ATCC 10876 (16.3 mm) were found to be the most sensitive organisms among the pathogens tested. © 2013 Sibi G et al. Source


Sibi G.,Robust Materials Technology Pvt. Ltd | Chatly P.,Robust Materials Technology Pvt. Ltd | Adhikari S.,Robust Materials Technology Pvt. Ltd | Ravikumar K.R.,Robust Materials Technology Pvt. Ltd
Research Journal of Medicinal Plant | Year: 2012

Morinda citrifolia L., also known as noni or Indian mulberry is a small evergreen tree having antimicrobial, antitumor, antidepressant and immune enhancing effects. Various parts of M. citrifolia have been investigated for its phytochemical and antimicrobial properties. In the present study, methanolic extracts of leaf, stem and roots of M. citrifolia has been prepared and analyzed for their phytoconstituents. Qualitative analysis of the extracts revealed the presence of phenols, tannins, saponins, alkaloids, glycosides, flavonoids and steroids at various levels. Further, the extracts were tested against pathogenic bacterial and fungal strains at different concentrations to determine the influence of phytochemicals. The results revealed that root and leaf extracts has significant antimicrobial activities mainly due to phenolics and tannins. Staphylococcus epidermidis and Pseudomonas aeruginosa, major pathogens of nosocomial infections were effectively controlled by the extracts at various concentrations and root extract exhibited significant activity against Penicillium sp. and Fusarium sp. It has been revealed that, the bioactive substances have influenced the antimicrobial properties of M. citrifolia which could be exploited to formulate novel drugs from plant origin against bacterial and fungal infections. © 2012 Academic Journals Inc. Source


Sibi G.,Robust Materials Technology Pvt. Ltd | Wadhavan R.,Robust Materials Technology Pvt. Ltd | Singh S.,Robust Materials Technology Pvt. Ltd | Shukla A.,Robust Materials Technology Pvt. Ltd | And 3 more authors.
Pakistan Journal of Biological Sciences | Year: 2013

Bioactive compounds from plant latex are potential source of antifungic against post harvest pathogens. Latex from a total of seven plant species was investigated for its phytochemical and antifungal properties. Six fungi namely Aspergillus fumigatus, A. niger, A. terreus, F. solani, P. digitatum and R. arrhizus were isolated from infected fruits and vegetables and tested against various solvent extracts of latex. Analysis of latex extracts with phytochemical tests showed the presence of alkaloids, flavonoids, glycosides, phenols, saponins, steroids, tannins and terpenoids. Antifungal assay revealed the potential inhibitory activity of petroleum ether extracts against the postharvest fungal isolates. Various degree of sensitivity was observed irrespective of plant species studied with A. terreus and P. digitatum as the most susceptible ones. F. solani and A. fumigatus were moderately sensitive to the latex extracts tested. Among the plants, latex of Thevetia peruviana (75.2%) and Artocarpus heterophyllus (64.8%) were having potential antifungal activity against the isolates followed by Manilkara zapota (51.1%). In conclusion, use of plant latex makes interest to control postharvest fungal diseases and is fitting well with the concept of safety for human health and environment. © 2013 Asian Network for Scientific Information. Source


Sibi G.,Robust Materials Technology Pvt. Ltd | Wadhavan R.,Robust Materials Technology Pvt. Ltd | Singh S.,Robust Materials Technology Pvt. Ltd | Dhananjaya K.,Robust Materials Technology Pvt. Ltd | And 2 more authors.
Asian Journal of Pharmaceutical and Clinical Research | Year: 2013

Black mold disease caused by Aspergillus niger is the predominant infection occurs in onions (Allium cepa L.). Use of synthetic fungicides to control plant diseases is a common practice but their toxicity and development of pathogen resistance needs to find alternative sources for disease management. Spices have been known to have potential antimicrobial properties and this study has been focused to control onion black mold using Indian culinary spices under in vitro conditions. Various parts of fourteen Indian culinary spices have been selected and phytochemical analysis was performed from the methanolic extracts. Aspergillus niger was isolated from infected onion bulbs and antifungal assay was performed under in vitro conditions. Phytochemical tests revealed that glycosides, steroids and terpenoids were the major phytochemicals universally present in the samples followed by flavonoids, tannins and phenols. Preliminary antifungal screening revealed that among fourteen spices tested, cardamom, cinnamon, clove, pepper, star anise and stone flower were exhibited inhibitory activity against the black mold. Various concentrations of the extracts ranging from 15 to 120 mg/ml were prepared and minimum inhibitory concentration (MIC) values were determined. Significant inhibitory activity was found at 15mg/ml concentration for cinnamon and clove. Stone flower at 30mg/ml was able to inhibit the pathogen and moderate inhibition was found in cardamom. Source


Dhananjaya K.,Robust Materials Technology Pvt. Ltd | Sibi G.,Robust Materials Technology Pvt. Ltd | Mallesha H.,Robust Materials Technology Pvt. Ltd | Ravikumar K.R.,Robust Materials Technology Pvt. Ltd | Awasthi S.,Robust Materials Technology Pvt. Ltd
Asian Pacific Journal of Tropical Biomedicine | Year: 2012

Objective: To evaluate potential use of daidzein and genistein in the prevention of breast cancer. Methods: Molecular docking of human estrogen receptor α (HER α) with daidzein and genistein were done by using Discover studio 3.1. Various analogs of daidzein and genistein were docked with HER α to enhance the binding affinity and locate the pharmacophoric groups. Results: Replacement of pharmacophoric group with methylsulphonmide (-NH-SO2-CH3) in daidzein and cyanide (-CN) in genistein were found to be sterically more compatible with significant increased energy values and could serve as probable lead molecules. The docking sore (E-value) obtained for genistein and daidzein was -217.67 and -220.63 respectively which shows their high affinity for HERα. Conclusions: Lower affinity and the side effects associated with conventional drugs needs an improved solution. The present findings showed that daidzein and genistein can play a potent role in breast cancer prevention. © 2012 Asian Pacific Tropical Biomedical Magazine. Source

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