RN Tagore International Institute of Cardiac science

Mukundapur, India

RN Tagore International Institute of Cardiac science

Mukundapur, India
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Ghosh D.,University of Calcutta | Firdaus S.B.,University of Calcutta | Mitra E.,University of Calcutta | Dey M.,University of Calcutta | And 5 more authors.
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2013

Objectives: Aim of the study is to find therapeutic potentials of aqueous Curry Leaf (Murraya koenigii) Extract (CuLE) against lead induced oxidative damage in hepatic tissue. The objectives are to study the alterations of various stress parameters in lead induced hepatotoxicity and amelioration of the same with CuLE. Methods: Rats were intraperitoneally injected with lead acetate (15mg/kg body weight). Another group was pre-treated with CuLE (50 mg / kg, fed orally).The positive control group was fed CuLE (50 mg / kg), and the control animals received vehicle treatment i.p. for 7 consecutive days. Concentration of lead in liver was estimated by AAS study. The alterations in the activity of the different bio-markers of hepatic damage, biomarkers of oxidative stress, activities of the antioxidant and some of the mitochondrial enzymes were studied. Histomorphology and alteration in tissue collagen level was studied through H-E staining and Sirius red staining respectively. Quantification of tissue collagen content was evaluated using confocal microscopy. Results: Lead caused alterations in all the parameters studied. All these changes were mitigated when the rats were pre-treated with CuLE. Concentration of lead in liver tissue was also decreased following pre-treatment with CuLE. Conclusions: The results indicate that the CuLE ameliorates lead-induced hepatic damage in experimental rats by antioxidants present in the extract. CuLE may have future therapeutic relevance in the prevention of lead-induced hepatotoxicity in humans exposed occupationally or environmentally to this toxic heavy metal and may be used for development of new hepatoprotective drugs of herbal origin with less cytotoxic effects.


Ghosh D.,University of Calcutta | Mitra E.,University of Calcutta | Firdaus S.B.,University of Calcutta | Ghosh A.K.,University of Calcutta | And 3 more authors.
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2013

Objective: The objectives of the present studies is to find out whether melatonin is capable of providing protection to rat heart against lead acetate induced oxidative damage. Methods: Rats of the first group were intraperitoneally (i.p.) injected with lead acetate [15mg/kg body weight(bw)], another group was pre-treated with melatonin (10 mg / kg, fed orally), the positive control group was fed melatonin (10 mg / kg bw), and the control animals received vehicle treatment i.p. for 7 consecutive days. Concentration of lead in cardiac tissue was estimated by AAS. The alterations in the activity of the different bio-marker enzymes of cardiac damage, levels of biomarkers of oxidative stress, activities of the antioxidant and some of the mitochondrial enzymes were studied. Histomorphological changes and alteration in tissue collagen level was also studied through H-E and Sirius red stainings respectively. Results: Treatment of rats with lead acetate at the indicated dose for seven consecutive days caused significant accumulation of lead in cardiac tissue, alterations of all the parameters studied and caused injury to the cardiac tissue. All these changes were ameliorated when the rats were pre-treated with melatonin. Conclusion: The results of the current studies indicate melatonin's ability to mitigate lead-induced oxidative damage in cardiac tissue of experimental rats possibly through its antioxidant mechanisms, and, may have future therapeutic relevance in humans exposed to lead environmentally or occupationally and in situations where chelation therapy has limited success.


Ghosh D.,University of Calcutta | Firdaus S.B.,University of Calcutta | Mitra E.,University of Calcutta | Chattopadhyay A.,Lane College | And 3 more authors.
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2013

Objective: Aim of the study is to find therapeutic potentials of aqueous extract of Murraya koenigii (CuLE) against lead induced oxidative damage in renal tissues of rats. Methods: Rats were intraperitoneally (i.p.) injected with lead acetate (15mg/kg body weight), another group was pre-treated with CuLE (50 mg / kg, fed orally), the positive control group was fed CuLE (50 mg / kg), and the control animals received vehicle treatment (i.p.) for 7 consecutive days. Concentration of lead in renal tissue was quantified using AAS study. The changes in the activity of the different bio-markers of renal damage, biomarkers of oxidative stress, activities of the antioxidant and some of the mitochondrial enzymes were studied. Histomorphology was studied through H-E staining and alteration in tissue collagen level was studied through Sirius red staining and confocal imaging. Results: Lead acetate caused changes in all the parameters evaluated. All these changes were ameliorated when the rats were pre-treated with CuLE. Concentrations of lead in kidneys were also decreased following pre-treatment of rats with CuLE. Conclusion: CuLE has the potentiality to protect against lead acetate-induced oxidative stress mediated nephrotoxicity in rat possibly through its antioxidant activity. CuLE may have future therapeutic relevance in lead acetate-induced nephrotoxiity in human who are environmentally or occupationally exposed to lead.


Dutta M.,University of Calcutta | Dutta M.,Vidyasagar College | Ghosh D.,University of Calcutta | Ghosh A.K.,University of Calcutta | And 8 more authors.
Food and Chemical Toxicology | Year: 2014

Arsenic is a well known global groundwater contaminant. Exposure of human body to arsenic causes various hazardous effects via oxidative stress. Nutrition is an important susceptible factor which can affect arsenic toxicity by several plausible mechanisms. Development of modern civilization led to alteration in the lifestyle as well as food habits of the people both in urban and rural areas which led to increased use of junk food containing high level of fat. The present study was aimed at investigating the effect of high fat diet on heart and liver tissues of rats when they were co-treated with arsenic. This study was established by elucidating heart weight to body weight ratio as well as analysis of the various functional markers, oxidative stress biomarkers and also the activity of the antioxidant enzymes. Histological analysis confirmed the biochemical investigations. From this study it can be concluded that high fat diet increased arsenic induced oxidative stress. © 2014 Elsevier Ltd.


Ghosh D.,University of Calcutta | Mitra E.,University of Calcutta | Dey M.,University of Calcutta | Firdaus S.B.,University of Calcutta | And 6 more authors.
Asian Journal of Pharmaceutical and Clinical Research | Year: 2013

Treatment of rats with lead acetate at a dose of 15 mg / kg body weight intraperitoneally (i.p) for seven consecutive days caused a significant accumulation of lead with concomitant damage in the rat hepatic and renal tissues indicated by the increase in the level of activities of SGPT and serum lactate dehydrogenase 5, levels of blood creatinine and serum bilirubin. Histological studies confirmed the damages and those were caused due to oxidative stress was evident from the changes observed in the levels of lipid peroxidation and reduced glutathione, and the alterations in the activities of hepatic and renal antioxidant and pro-oxidant enzymes. Lead acetate treatment also caused alterations in the activities of mitochondrial Kreb's cycle and respiratory chain enzymes. All these changes were ameliorated when the rats were pre-treated with melatonin at a dose of 10 mg / kg (fed orally) for a similar period of time. Melatonin is a ubiquitous indole amine present naturally in plants and animals, recognized as a neurohormone secreted from the vertebrate pineal gland which has role in maintaining biorhythm and is also a potent antioxidant nutrient. The current studies indicated that melatonin protected the rat hepatic and renal tissues against lead-induced oxidative stress possibly through its antioxidant activity. As melatonin is present in almost all organisms and in many food items including cereals, green vegetables and fruits, it may be considered as one of the important component of the regular diet. Moreover, pharmacologically administered melatonin has been reported to be well tolerated in humans with no reported side-effects. The results of the current studies seem to have relevance at places where humans are exposed to lead environmentally or occupationally and where chelation therapy has limited success.


Mukherjee D.,Sudan University of Science and Technology | Ghosh A.K.,Sudan University of Science and Technology | Bandyopadhyay A.,Indian Institute of Chemical Technology | Basu A.,Sudan University of Science and Technology | And 4 more authors.
Journal of Pineal Research | Year: 2012

The present study was undertaken to explore the protective effect of melatonin against isoproterenol bitartrate (ISO)-induced rat myocardial injury and to test whether melatonin has a role in preventing myocardial injury and recovery when the ISO-induced stress is withdrawn. Treatment for rats with ISO altered the activities of some of the key mitochondrial enzymes related to energy metabolism, the levels of some stress proteins, and the proteins related to apoptosis. These changes were found to be ameliorated when the animals were pretreated with melatonin at a dose of 10 mg/kg BW, i.p. In addition to its ability to reduce ISO-induced mitochondrial dysfunction, we also studied the role of melatonin in the recovery of the cardiac tissue after ISO-induced damage. Continuation of melatonin treatment in rats after the withdrawal of ISO treatment was found to reduce the activities of cardiac injury biomarkers including serum glutamate oxaloacetate transaminase (SGOT), lactate dehydrogenase (LDH), and cardio-specific LDH1 to control levels. The levels of tissue lipid peroxidation and reduced glutathione were also brought back to that seen in control animals by continued melatonin treatment. Continuation of melatonin treatment in post-ISO treatment period was also found to improve cardiac tissue morphology and heart function. Thus, the findings indicate melatonin's ability to provide cardio protection at a low pharmacological dose and its role in the recovery process. Melatonin, a molecule with very low or no toxicity may be considered as a therapeutic for the treatment for ischemic heart disease. © 2012 John Wiley & Sons A/S.


Sen S.,RN Tagore International Institute of Cardiac science | Bandyopadhyay B.,RN Tagore International Institute of Cardiac science | Eriksson P.,Sahlgrenska University Hospital | Chattopadhyay A.,RN Tagore International Institute of Cardiac science
Congenital Heart Disease | Year: 2012

Objective. Previous studies have seldom compared functional capacity in children following Fontan procedure alongside those with Glenn operation as destination therapy. We hypothesized that Fontan circulation enables better midterm submaximal exercise capacity as compared to Glenn physiology and evaluated this using the 6-minute walk test. Design and Patients. Fifty-seven children aged 5-18 years with Glenn (44) or Fontan (13) operations were evaluated with standard 6-minute walk protocols. Results. Baseline SpO2 was significantly lower in Glenn patients younger than 10 years compared to Fontan counterparts and similar in the two groups in older children. Postexercise SpO2 fell significantly in Glenn patients compared to the Fontan group. There was no statistically significant difference in baseline, postexercise, or postrecovery heart rates (HRs), or 6-minute walk distances in the two groups. Multiple regression analysis revealed lower resting HR, higher resting SpO2, and younger age at latest operation to be significant determinants of longer 6-minute walk distance. Multiple regression analysis also established that younger age at operation, higher resting SpO2, Fontan operation, lower resting HR, and lower postexercise HR were significant determinants of higher postexercise SpO2. Younger age at operation and exercise, lower resting HR and postexercise HR, higher resting SpO2 and postexercise SpO2, and dominant ventricular morphology being left ventricular or indeterminate/mixed had significant association with better 6-minute work on multiple regression analysis. Lower resting HR had linear association with longer 6-minute walk distances in the Glenn patients. Conclusions. Compared to Glenn physiology, Fontan operation did not have better submaximal exercise capacity assessed by walk distance or work on multiple regression analysis. Lower resting HR, higher resting SpO2, and younger age at operation were factors uniformly associated with better submaximal exercise capacity. © 2012 Wiley Periodicals, Inc.


Mitra E.,University of Calcutta | Ghosh A.K.,University of Calcutta | Ghosh D.,University of Calcutta | Mukherjee D.,University of Calcutta | And 4 more authors.
Food and Chemical Toxicology | Year: 2012

Treatment of rats with a low dose of cadmium chloride caused a significant damage in the rat cardiac tissue indicated by the increase in the level of serum glutamate oxaloacetate transaminase and lactate dehydrogenase1 activities. Histological studies confirmed the damage due to cadmium. That cadmium-induced tissue damage was caused due to oxidative stress was evident from the changes observed in the levels of lipid peroxidation and reduced glutathione, the protein carbonyl content, and the alterations in the activities of cardiac antioxidant and pro-oxidant enzymes. Treatment of rats with cadmium also caused alterations in the activities of mitochondrial Kreb's cycle as well as respiratory chain enzymes. All these changes were ameliorated when the rats were pre-treated with an aqueous extract of Curry leaf (Murraya koenigii). The studies indicated that the aqueous extract of Curry leaf protects the rat cardiac tissue against cadmium-induced oxidative stress possibly through its antioxidant activity. As curry leaf is consumed by people as part of their diet in India and South-East Asian and some European countries as well, and, as it has no reported side-effects, the results seem to have relevance at places where humans are exposed to cadmium environmentally or occupationally. © 2012 Elsevier Ltd.


PubMed | RN Tagore International Institute of Cardiac science
Type: Comparative Study | Journal: Congenital heart disease | Year: 2012

Previous studies have seldom compared functional capacity in children following Fontan procedure alongside those with Glenn operation as destination therapy. We hypothesized that Fontan circulation enables better midterm submaximal exercise capacity as compared to Glenn physiology and evaluated this using the 6-minute walk test.Fifty-seven children aged 5-18 years with Glenn (44) or Fontan (13) operations were evaluated with standard 6-minute walk protocols.Baseline SpO(2) was significantly lower in Glenn patients younger than 10 years compared to Fontan counterparts and similar in the two groups in older children. Postexercise SpO(2) fell significantly in Glenn patients compared to the Fontan group. There was no statistically significant difference in baseline, postexercise, or postrecovery heart rates (HRs), or 6-minute walk distances in the two groups. Multiple regression analysis revealed lower resting HR, higher resting SpO(2) , and younger age at latest operation to be significant determinants of longer 6-minute walk distance. Multiple regression analysis also established that younger age at operation, higher resting SpO(2) , Fontan operation, lower resting HR, and lower postexercise HR were significant determinants of higher postexercise SpO(2) . Younger age at operation and exercise, lower resting HR and postexercise HR, higher resting SpO(2) and postexercise SpO(2) , and dominant ventricular morphology being left ventricular or indeterminate/mixed had significant association with better 6-minute work on multiple regression analysis. Lower resting HR had linear association with longer 6-minute walk distances in the Glenn patients.Compared to Glenn physiology, Fontan operation did not have better submaximal exercise capacity assessed by walk distance or work on multiple regression analysis. Lower resting HR, higher resting SpO(2) , and younger age at operation were factors uniformly associated with better submaximal exercise capacity.


PubMed | University of Calcutta, RN Tagore International Institute of Cardiac science and Vidyasagar College
Type: | Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association | Year: 2014

Arsenic is a well known global groundwater contaminant. Exposure of human body to arsenic causes various hazardous effects via oxidative stress. Nutrition is an important susceptible factor which can affect arsenic toxicity by several plausible mechanisms. Development of modern civilization led to alteration in the lifestyle as well as food habits of the people both in urban and rural areas which led to increased use of junk food containing high level of fat. The present study was aimed at investigating the effect of high fat diet on heart and liver tissues of rats when they were co-treated with arsenic. This study was established by elucidating heart weight to body weight ratio as well as analysis of the various functional markers, oxidative stress biomarkers and also the activity of the antioxidant enzymes. Histological analysis confirmed the biochemical investigations. From this study it can be concluded that high fat diet increased arsenic induced oxidative stress.

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