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Bhosari, India

Topre S.D.,RJSPMs College | Panikar S.S.,RJSPMs College | Mahajani S.U.,Lokmangal Biotechnology College | Patil S.B.,University of Pune
Journal of Pure and Applied Microbiology | Year: 2011

Two isolates of agriculturally important nitrogen fixing microbes, Rhizobium meliloti and Azospirillium lipoferum were isolated from Western Maharashtra soil habitat. The standard isolation technique was carried out in laboratory and were characterized by 16SrDNA method. The growth of microbes was studied at different pH and the optimal pH was found to be 6.5 and 7 for Bhizobium meliloti and Azospirillium lipoferum respectively. The nodulation activity of Bhizobium meliloti was confirmed. Carrier plays an important role in maintaining sufficient shelf life so survival of microorganisms in different carriers at 28°C was deduced and lignite was found to be the most efficient carrier for Bhizobium meliloti and Azospirillium lipoferum. Further these microorganisms were multiplied for mass production using Lignite as carrier. The biofertilizer was checked for colony count to meet the standard requirement (i.e. 107 CFU/gm). Source

Wakhloo D.,RJSPMs College | Mahajani S.,Italian Institute of Technology | Tembe P.,RJSPMs College | Raut A.,RJSPMs College
Journal of Pure and Applied Microbiology | Year: 2013

Probiotics are live microorganisms which are regularly consumed as part of our daily diet and help to protect the host from various pathogens. The mechanisms by which the indigenous intestinal bacteria inhibit pathogens include competition for colonization sites and nutrients, production of toxic compounds, and stimulation of the immune system. Lactobacillus plantarum (C18) bacterial strain was isolated from yogurt and observed to be gram positive rod-shaped bacilli. After carrying out auto-aggregation and co-aggregation assay, it was seen that it displayed a strong auto-aggregating and co-aggregating phenotype. A known probiotic bacteria, Streptococcus cremoris was used as a control. Comparison studies proved that L.plantarum bacterial strain showed higher auto-aggregating and co-aggregating properties as compared to S. cremoris. Therefore, if S. cremoris is said to be one of the ideal candidates for probiotic use, this strain of bacteria could challenge the definition of ideal requirements for probiotic bacteria. Hypothesis could state that cells when cultured in broth secrete certain proteins which help in bacterial adhesion to the intestinal tract cells, and this was seen to be higher in supernatant diluted C18 bacterial culture than in saline diluted counterpart. Source

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