Ren G.,University of Macau |
Sha T.,Rizhao Hospital of TCM |
Guo J.,University of Macau |
Li W.,University of Macau |
And 2 more authors.
Journal of Natural Medicines | Year: 2015
Cucurbitacin B (Cuc B), a natural compound extracted from cucurbitaceous plants, demonstrated potent anticancer activities, while the underlying mechanisms remain unclear. We investigated the anticancer effect of Cuc B on MCF-7 breast cancer cells. Cuc B drastically decreased cell viability in a concentration-dependent manner. Cuc B treatment caused DNA damage, as shown by long tails in the comet assay and increased γH2AX protein expression. Immunofluorescence staining showed that Cuc B treatment induced nuclear γH2AX foci. Cuc B activated DNA damage pathways by phosphorylation of ATM/ATR [two large phosphatidylinositol-3-kinase-like kinase family (PIKKs) members]. Furthermore, it also induced autophagy, as evidenced by monodansylcadaverine (MDC) staining and autophagic protein expression. In addition, Cuc B treatment led to increased reactive oxygen species (ROS) formation, which was inhibited by N-acetyl-l-cysteine (NAC) pretreatment. NAC pretreatment inhibited Cuc-B-induced DNA damage and autophagy. Taken together, these results suggest that ROS-mediated Cuc-B-induced DNA damage and autophagy in MCF-7 cells, which provides new insights into the anticancer molecular mechanism of Cuc B. © 2015 The Japanese Society of Pharmacognosy and Springer Japan.
Sun R.,Shandong Academy of Chinese Medicine |
Feng Q.,Tianjin University of Traditional Chinese Medicine |
Xie Y.-Z.,Yantai Rongchang Pharmaceutical Co. |
Yang Q.,Rizhao Hospital of TCM |
Li S.-J.,U.S. Food and Drug Administration
Zhongguo Zhongyao Zazhi | Year: 2015
Models of throat excess-heat mice were established and different dosages of water extract of STRR were ig given to mice to observe anti-inflammatory effect and its mechanism. The activities of ALT, AST and the contents of TNF-α, T3, rT3, T4, SOD, MDA, PEG2, NO, NOS, Cr, BUN, GSH and GSH-Px in serum were tested while liver index, kidney index, spleen index and thymus index were measured. The anti-inflammatory efficacy accompanied by side effects and mechanisms of water extract of Sophorae Tonkinensis Radix et Rhizoma (STRR) in excess-heat mice were investigated to clear safety dose-dependence range and the relationship of efficacy, toxicity and syndrome. In the experiment, water extract of STRR showed a strong inhibitory effect on ear edema by croton oil in throat excess-heat mice. The activities of ALT, AST in serum and liver index were all higher than that of normal group after multiple administration. PEG2, SOD, MDA, NO, NOS, GSH and GSH-Px had obvious changes. According to the results, water extract of STRR has an anti-inflammatory effect on acute inflammation in throat excess-heat mice and it is stronger than that in normal mice. The anti-inflammatory effect of STRR is related to the reduction of inflammatory mediators release. Side effects and hepatotoxicity will be produced on clinical efficacy dosage. The mechanisms of anti-inflammation and hepatotoxicity are all in connection with oxidative damnification.
Wang X.,Linyi Normal University |
Wang R.,Rizhao Hospital of TCM |
Liu Z.,Peoples Hospital of Rizhao |
Hao F.,Qingdao University |
Huang H.,Linyi Peoples Hospital
International Journal of Clinical and Experimental Pathology | Year: 2015
Identifying prognostic factors for osteosarcoma (OS) aids in the selection of patients who require more aggressive management. XB130 is a newly characterized adaptor protein that was reported to be a prognostic factor of certain tumor types. However, the association between XB130 expression and the prognosis of OS remains unknown. In the present study, we investigated the association between XB130 expression and clinicopathologic features and prognosis in patients suffering OS, and further investigated its potential role on OS cells in vitro and vivo. A retrospective immunohistochemical study of XB130 was performed on archival formalin-fixed paraffin-embedded specimens from 60 pairs of osteosarcoma and noncancerous bone tissues, and compared the expression of XB130 with clinicopathological parameters. We then investigate the effect of XB130 sliencing on invasion in vitro and lung metastasis in vivo of the human OS cell line. Immunohistochemical assays revealed that XB130 expression in OS tissues was significantly higher than that in corresponding noncancerous bone tissues (P = 0.001). In addition, high XB130 expression more frequently occurred in OS tissues with advanced clinical stage (P = 0.002) and positive distant metastasis (P = 0.001). Moreover, OS patients with high XB130 expression had significantly shorter overall survival and disease-free survival (both P < 0.001) when compared with patients with the low expression of XB130. The univariate analysis and multivariate analysis shown that high XB130 expression and distant metastasis were the independent poor prognostic factor.We showed that XB130 depletion by RNA interference inhibited invasion of XB130-rich U2OS cells in vitro and lung metastasis in vivo. This is the first study to reveal that XB130 overexpression may be related to the prediction of metastasis potency and poor prognosis for OS patients, suggesting that XB130 may serve as a prognostic marker for the optimization of clinical treatments. Furthermore, XB130 is the potential molecular target for OS therapy.
Pore cranial drill as a simple and quick approach for ventricular hemorrhage management: Technical note [Ventriküler Kanaman?n Tedavisinde Basit ve H?zl? Bir Yakla??m Olarak Kraniyal Delikçik Delgi: Teknik Not]
Jin P.,Qingdao University |
Fei C.,Linyi Peoples Hospital |
Sun X.,Rizhao Hospital of TCM |
Bai Y.,Yidu Central Hospital |
And 4 more authors.
Journal of Neurological Sciences | Year: 2014
Objectives:To increase the successful rate of cure and decrease the severe disability rate and death rate, the rapid pore cranial drill was used in the treatment of ventricular hemorrhage caused by thalamic hemorrhage. The clinical effects and experiences of using rapid pore cranial drill with external ventricular drainage (EVD) were summarized, and the significance of pore cranial drill was assessed. Methods: 401 cases of ventricular hemorrhage caused by thalamic hemorrhage between May 1983 and December 2010 were retrospectively analyzed. All cases were performed EVD with pore cranial drill (including infusion with urokinase). There were 212 males and 189 females who ranged in age from 19 to 78 years (mean 65.2 years). The technique details with this drill were described. Results: At one month following the operations, there were 147 cases (36.7%) showing cured, 192 (47.9%) showing improvement, and the remaining 62 (15.4%) showing either against-advice discharge or died. The prognoses were evaluated based on the activity of daily living (ADL) after the 1-3 months treatment. Two typical cases were presented with detailed histories and imaging data. Conclusion: Pore cranial drill with EVD (including infusion with urokinase) is a simple and quick treatment approach for ventricular hemorrhage caused by thalamic hemorrhage. The technique has the advantage of making full use of every minute to rescue the patients of severe craniocerebral diseases.
Zhang M.,Shandong Medical Imaging Research Institute |
Zhao B.,Shandong Medical Imaging Research Institute |
Wang Y.,Shandong Medical Imaging Research Institute |
Chen W.,Rizhao Hospital of TCM |
Hou L.,Rizhao Hospital of TCM
Health Physics | Year: 2012
In radiographic examination, not all medical imaging tasks require the same level of image quality or diagnostic information. Criteria should be established for different imaging tasks to avoid excessive doses where there is no clear net benefit in the diagnosis or the image quality. An exposure index provided by manufacturers would be a useful tool for this purpose. This study aims to establish an optimum exposure index to be used as a guideline for clinical imaging tasks to minimize radiation exposure for chest digital radiography. A three-level classification of image quality (high, medium, and low) for chest imaging tasks was carried out. An anthropomorphic phantom was employed to establish minimum exposure index and exposure (mAs) for clinical imaging task type I (corresponding to high image quality). The exposures of medium and low quality images derived from it. Thirty patients were exposed consecutively with these optimized exposure factors, and clinical tasks were considered, while another 30 patients were exposed with the exposure factors routinely used in practice. Image quality was assessed objectively by a consensus panel. The optimized exposure provided a significant reduction of the mean exposure index from 1,556 to 1,207 (p < 0.0001) and mean patient's entrance surface dose from 0.168 mGy to 0.092 mGy (p < 0.0001). The results show that a clinical-task-determined radiographic procedure is more conducive to radiation protection of patients. In this study, the posteroanterior chest imaging examination was chosen as an example. This procedure can also apply to other body parts and views. Copyright © 2012 Health Physics Society.