PubMed | Beijing Normal University, Rizhao City Peoples Hospital, University of Science and Technology of China and Beijing Hospital and Beijing Institute of Geriatrics
Type: Journal Article | Journal: Oncology reports | Year: 2016
In previous studies, we demonstrated that rhein lysinate (RHL), the salt of rhein and lysine that is easily dissolved in water, inhibited the growth of tumor cells derived from breast and ovarian cancer, hepatocellular carcinoma, cervical cancer and lung carcinoma. Based on these observations, human glioma U87 cells and a xenograft model in BALB/c nude mice were used to examine the antitumor activity of RHL against human glioma. Notably, RHL statistically significantly suppressed the growth of human glioma U87 xenografts in BALB/c nude mice. In vitro, there was a significant reduction in cell proliferation after treatment with RHL in a dose- and time-dependent manner. The overall growth inhibition was correlated with the increase in reactive oxygen species (ROS) production and cell apoptosis. The apoptosis- and cell cycle-related proteins including BAX and Bim were increased, whereas Bcl-2 and cyclin D were decreased in the RHL-treated cells. The results demonstrated that RHL is highly effective against the growth of human glioma U87 xenografts in BALB/c nude mice. The potent antitumor activity of RHL may be mediated through downregulation of Bcl-2 and cyclin D expression and upregulation of BAX and Bim expression.
PubMed | Rizhao City Peoples Hospital, Taishan Medical University and Laiwu City Peoples Hospital
Type: | Journal: Fitoterapia | Year: 2015
In this research, a sensitive and reliable LC-MS/MS method was developed and applied to determine the concentration of pristimerin in rat plasma, cell incubation media and metabolism incubation mixtures. The absolute oral bioavailability of pristimerin is 28.4% at a dose of 1 mgkg(-1), and the bioavailability was poor. The bidirectional transport of pristimerin across Caco-2 cells was studied in vitro. A markedly higher transport of pristimerin across Caco-2 cells was observed in the basolateral-to-apical direction and was abrogated in the presence of the P-gp inhibitor, verapamil. The result indicated that P-gp might be involved in the transport of pristimerin in intestine. The phase I and phase II metabolic stability was also investigated using human liver microsomes (HLM) and S9 fractions, respectively. Pristimerin was stable in S9 fractions but metabolized in HLM with a half-life of 20.4 min, which indicated that pristimerin could be mainly metabolized by phase I enzymes. In conclusion, the absolute oral bioavailability of pristimerin in plasma, transport across Caco-2 cell monolayers, and metabolic stability in HLM and S9 fractions were systematically investigated by using a sensitive and reliable LC-MS/MS method.
PubMed | Rizhao City Peoples Hospital and Qingdao University
Type: | Journal: Medical science monitor : international medical journal of experimental and clinical research | Year: 2015
Magnetic resonance angiography (MRA) and diffusion-weighted imaging (DWI) have been widely used in the prediction of ischemic stroke; however, the differences of the 2 methods in detection the artery lesion differences between transient ischemic attack (TIA) and infarction patients have been long neglected. We performed the present study to investigate the differences between vessel characteristics detected by MRA and DWI in acute stroke and TIA patients.We classified 110 subjects into 2 groups and all the patients underwent both MRA and DWI. The degree of stenosis of cranial and cervical arteries, the distribution of the stenosis, the development and changes of the vessels, and the DWI scanning results of the brain tissue were all analyzed.We detected a significant difference in the number and the degree of stenosis of cranial and cervical arteries among the 3 groups (P=0.006). Compared with health controls, patients with TIA and cerebral infraction had much more severe stenosis and occlusive arteries (P<0.05). However, no significant difference was detected between TIA and cerebral infraction patients (P=0.148). Moreover, a higher rate of unilateral vertebral artery dysplasia was found in the vertebrobasilar TIA patients. Higher lesion signals were also observed by DWI in TIA patients of internal carotid artery system (4/8, 50%).Vessel characteristics were not significantly different between TIA and infarction patients. Unilateral vertebral artery hypoplasia was a predisposing factor for vertebrobasilar TIA and ischemic focus in DWI detection was always caused by severe artery lesions.
Zhang G.,Capital Medical University |
Jin G.,Capital Medical University |
Nie X.,Rizhao City Peoples Hospital |
Mi R.,Capital Medical University |
And 3 more authors.
PLoS ONE | Year: 2014
Viruses have demonstrated strong potential for the therapeutic targeting of glioblastoma stem cells (GSCs). In this study, the use of a herpes simplex virus carrying endostatin-angiostatin (VAE) as a novel therapeutic targeting strategy for glioblastoma-derived cancer stem cells was investigated. We isolated six stable GSC-enriched cultures from 36 human glioblastoma specimens and selected one of the stable GSCs lines for establishing GSC-carrying orthotopic nude mouse models. The following results were obtained: (a) VAE rapidly proliferated in GSCs and expressed endo-angio in vitro and in vivo 48 h and 10 d after infection, respectively; (b) compared with the control gliomas treated with rHSV or Endostar, the subcutaneous gliomas derived from the GSCs showed a significant reduction in microvessel density after VAE treatment; (c) compared with the control, a significant improvement was observed in the length of the survival of mice with intracranial and subcutaneous gliomas treated with VAE; (d) MRI analysis showed that the tumor volumes of the intracranial gliomas generated by GSCs remarkably decreased after 10 d of VAE treatment compared with the controls. In conclusion, VAE demonstrated oncolytic therapeutic efficacy in animal models of human GSCs and expressed an endostatin-angiostatin fusion gene, which enhanced antitumor efficacy most likely by restricting tumor microvasculature development. © 2014 Zhang et al.
PubMed | Rizhao City Peoples Hospital, Capital Medical University and University of British Columbia
Type: Journal Article | Journal: PloS one | Year: 2014
Viruses have demonstrated strong potential for the therapeutic targeting of glioblastoma stem cells (GSCs). In this study, the use of a herpes simplex virus carrying endostatin-angiostatin (VAE) as a novel therapeutic targeting strategy for glioblastoma-derived cancer stem cells was investigated. We isolated six stable GSC-enriched cultures from 36 human glioblastoma specimens and selected one of the stable GSCs lines for establishing GSC-carrying orthotopic nude mouse models. The following results were obtained: (a) VAE rapidly proliferated in GSCs and expressed endo-angio in vitro and in vivo 48 h and 10 d after infection, respectively; (b) compared with the control gliomas treated with rHSV or Endostar, the subcutaneous gliomas derived from the GSCs showed a significant reduction in microvessel density after VAE treatment; (c) compared with the control, a significant improvement was observed in the length of the survival of mice with intracranial and subcutaneous gliomas treated with VAE; (d) MRI analysis showed that the tumor volumes of the intracranial gliomas generated by GSCs remarkably decreased after 10 d of VAE treatment compared with the controls. In conclusion, VAE demonstrated oncolytic therapeutic efficacy in animal models of human GSCs and expressed an endostatin-angiostatin fusion gene, which enhanced antitumor efficacy most likely by restricting tumor microvasculature development.
Xu W.,Rizhao City Peoples Hospital
International Journal of Genomics | Year: 2014
Asthma is characterized by recurrent episodes of wheezing, shortness of breath, chest tightness, and coughing. It is usually caused by a combination of complex and incompletely understood environmental and genetic interactions. We obtained gene expression data with high-throughput screening and identified biomarkers of children's asthma using bioinformatics tools. Next, we explained the pathogenesis of children's asthma from the perspective of gene regulatory networks: DAVID was applied to perform Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enriching analysis for the top 3000 pairs of relationships in differentially regulatory network. Finally, we found that HAND1, PTK1, NFKB1, ZIC3, STAT6, E2F1, PELP1, USF2, and CBFB may play important roles in children's asthma initiation. On account of regulatory impact factor (RIF) score, HAND1, PTK7, and ZIC3 were the potential asthma-related factors. Our study provided some foundations of a strategy for biomarker discovery despite a poor understanding of the mechanisms underlying children's asthma. © 2014 Wen Xu.
Sha L.,Rizhao City Peoples Hospital
Advances in Information Sciences and Service Sciences | Year: 2012
In recent years, orthopaedics surgical robot technology has become one hot topic. However, operational objectives are human beings, so it raises the request about the interactive technique between the doctor and surgical robot. It's a key problem that prevents the research and development of the orthopaedics surgical robot. With the improvement of virtual reality and virtual reinforcement technology, robot-assisted orthopaedics surgical robot technology becomes an available way to solve the problem. In this paper, we investigate the multiple attribute group decision making (MAGDM) problems for evaluating the computer assisted orthopedic surgery with linguistic information. We utilize the linguistic weighted averaging (LWA) operator and linguistic hybrid aggregation (LHA) operator to aggregate the linguistic information corresponding to each alternative and get the overall value of the computer assisted orthopedic surgery, then rank the e-commerce websites and select the most desirable one(s) by using the overall value of the alternative of the computer assisted orthopedic surgery. Finally, an illustrative example for evaluating the computer assisted orthopedic surgery is given to verify the developed approach and to demonstrate its practicality and effectiveness.
Lian-Sheng S.,Rizhao City Peoples Hospital |
Zhan-Guo S.,Rizhao City Peoples Hospital
Proceedings - 7th International Conference on Intelligent Computation Technology and Automation, ICICTA 2014 | Year: 2015
The objective of this article is to discuss the clinical application values of multi-slice CT (MSCT) and image post-processing technique in the diagnosis of occult rib fracture. Method: after chest X-ray, 40 cases of chest trauma patients done 16 layers spiral CT scan, and the data from 16 layers spiral CT would be via the workstation to do multiple planar reconstruction (MPR) and volume reconstruction (VR) to rebuild images combined with axial images to make the diagnosis of fracture. For suspected fracture line we can use curved planner reconstruction (CRP) to make a diagnosis, and evaluate the situation of intrathoracic tissue after trauma. Results: 40 patients by used MSCT reconstruction, MSCT and reconstruction images found all the fracture showed by X-ray, 29 patients who were showed in X-ray failed to clear 36 rib fractures, and at the same time found 1 sternal fracture and 3 clavicle fractures which X-ray undiscovered, 3 cases of small amount pleural effusion, 2 cases of pulmonary contusion, 1 case of hepatic contusion, and 2 lumbar transverse process fracture. 2 patients with fake shadow because of respiratory motion, but reconstruction images show obvious false fracture line. Conclusion: MSCT thin layer image with post-processing reconstruction image is better diagnosis method for traumatic chest rib occult fracture, basically can satisfy the need of clinical for trauma caused by fracture and soft tissue injuries, hemopneumothorax, pulmonary contusion of thoracic trauma. © 2014 IEEE.
Luan Y.,Shandong University |
Chao S.,Shandong University |
Ju Z.-Y.,Rizhao City Peoples Hospital |
Wang J.,Shandong University |
And 4 more authors.
International Immunopharmacology | Year: 2015
Background Baicalin has been shown to possess various pharmacological actions, a recent study revealed that baicalin can attenuate pulmonary hypertension and pulmonary vascular remodeling through the inhibition of pulmonary artery smooth muscle cell proliferation, however, the potential mechanism remains unexplored. In this study, we investigated the therapeutic effect of baicalin on a rat model of monocrotaline (MCT)-induced pulmonary arterial hypertension (PAH) and attempted to further clarify the possible mechanisms underlying the anti-inflammatory. Methods and results Our research showed that compared with MCT-induced PAH model rats, rats administered intragrastically with 100 mg/kg baicalin showed the following after two weeks: the right ventricular systolic pressure (RVSP) and the right ventricle/left ventricle plus septum (RV/LV + S) ratio were lower (P < 0.05); the intima thickening and luminal stenosis were improved (P < 0.05); the mRNA levels of tumor necrosis factor alpha (TNF-α), interleukin-11β (IL-1β), IL-6, and endothelin-1 (ET-1) were obviously reduced by quantitative reverse transcription-polymerase chain reaction (qRT-PCR); the protein expression of transforming growth factor-β1 (TGF-β1), intercellular cell adhesion molecule-1 (ICAM-1) and nuclear factor-κB (NF-κB) were significantly decreased (P < 0.05); and the expression of inhibitor of NF-κB (I-κB) was increased (P < 0.05) through immunohistochemical and western blot. Conclusion We studied the protective effects of baicalin against the lung and heart damage in experimental PAH rats; the therapeutic effects maybe through inhibiting vascular endothelial inflammatory response. © 2015 Published by Elsevier B.V.
PubMed | Rizhao City Peoples Hospital and Shandong University
Type: Journal Article | Journal: Molecular medicine reports | Year: 2014
The aim of the present study was to investigate the effect of bone marrowderived mesenchymal stem cells (BMSCs) in the treatment of lung injury in a mouse model of bronchopulmonary dysplasia (BPD) and examine the underlying mechanisms. A mouse model of BPD was created using continuous exposure to high oxygen levels for 14 days. BMSCs were isolated, cultured and then labeled with green fluorescent protein. Cells (1x106) were subsequently injected intravenously 1 h prior to high oxygen treatment. Animals were randomly divided into three groups (n=5 in each): Control group, BPD model group and BMSC injection group. At two weeks posttreatment, the expression of transforming growth factor1 (TGF1), vascular endothelial growth factor (VEGF) and von Willebrand factor (vWF) was detected using immunohistochemical staining and immunofluorescence. Compared with the BPD model group, the body weight, airway structure and levels of TGF1 and VEGF were significantly improved in the BMSCtreated group. Immunofluorescence observations indicated that BMSCs were able to differentiate into cells expressing vWF and VEGF, which are markers of vascular tissues. The present study demonstrated that intravenous injection of BMSCs significantly improved lung damage in a neonatal mouse model of BPD at 14 days following hyperoxiainduced injury. This provides novel information which may be used to guide further investigation into the use of stem cells in BPD.