Risk Assessment Laboratory for Agro products Beijing

Laboratory for, China

Risk Assessment Laboratory for Agro products Beijing

Laboratory for, China
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Man Y.,Beijing Academy of Agriculture and Forestry Sciences | Man Y.,Risk Assessment Laboratory for Agro Products Beijing | Man Y.,Beijing Municipal Key Laboratory of Agriculture Environment Monitoring | Liang G.,Beijing Academy of Agriculture and Forestry Sciences | And 15 more authors.
Toxins | Year: 2017

A rapid, portable, and semi-quantitative immunochromatographic strip was developed for rapid and visual detection of alternariol monomethyl ether (AME). For this purpose, the anti-AME monoclonal antibody (mAb) was prepared and identified. AME coupled to bovine serum albumin (BSA) via methyl 4-bromobutanoate was prepared as immunogen. The recoveries of AME in spiked cherry and orange fruits determined by competitive ELISA were 86.1% and 80.7%, respectively. A colloidal gold nanoparticle (CGN) and CGNs-mAb conjugate were synthesized, and on this basis, a competitive colloidal gold immunochromatographic strip was developed and applied to the detection of AME toxin in fruit samples. The intensity of red density of the test line (T line) is inversely proportional to AME concentration in the range 0.1–10 ng/mL. The visual limit of detection (LOD) of AME was found to be about 10 ng/mL. The semi-quantitative detection can be completed in 10 min. Moreover, the immunochromatographic strip has lower cross-reactivity with AME analogues, and it has a good stability performance (following 3 months of storage). Hence, the colloidal gold immunochromatographic strip could be used as a semi-quantitative tool for the on-site, rapid, and visual detection of AME in fruit. © 2017 by the authors. Licensee MDPI, Basel, Switzerland.


Wang M.,Beijing Research Center for Agricultural Standards and Testing | Wang M.,Risk Assessment Laboratory for Agro products Beijing | Jiang N.,Beijing Research Center for Agricultural Standards and Testing | Jiang N.,Risk Assessment Laboratory for Agro products Beijing | And 6 more authors.
Journal of Agricultural and Food Chemistry | Year: 2017

Early and late ripening sweet cherries were characterized for phenolic acids, and also their antioxidant capacity and potential antifungal effects were investigated. Free, conjugated, and bound phenolics were identified and quantified using ultra performance liquid chromatography-tandem mass spectrometry. Our results indicated that the early ripening cultivars contained higher free phenolic acids, which was positively related to remarkable antioxidant properties and the inhibition effects on Alternaria alternata and tenuazonic acid (TeA) accumulation. However, conjugated phenolics of the late ripening cultivars, mainly including caffeic, 2,3,4-trihydroxybenzoic, p-coumaric, and pyrocatechuic acids, achieved the highest antifungal effects and almost completely inhibited the A. alternata and TeA production. 2,2-Diphenyl-1-picrylhydrazyl testing and ferric ion reducing antioxidant power assay showed strong positive correlation with total phenolics and specific phenolics such as free epicatechin and conjugated 2,3,4-trihydroxybenzoic acids and also with antifungal activity. Results from this study provide further insights into the health-promoting phenolic compounds in sweet cherries. © 2017 American Chemical Society.


Li A.,Risk Assessment Laboratory for Agro products Beijing | Pan L.-G.,Risk Assessment Laboratory for Agro products Beijing | Zhang X.-T.,Risk Assessment Laboratory for Agro products Beijing | Jin X.-X.,Risk Assessment Laboratory for Agro products Beijing | Ma H.-Z.,Risk Assessment Laboratory for Agro products Beijing
Radiation Physics and Chemistry | Year: 2017

2-Dodecylcyclobutanone (2-DCB) is validated as a marker for irradiated foodstuffs. However, the preparation methods for the marker compound involve time-consuming and tedious procedures prior to analysis. This paper describes a rapid method for the extraction and cleanup of 2-DCB in irradiated pork. The highly automated extraction procedure is based on accelerated solvent extraction combined with a purification step by Al2O3, which is added to the extraction cell. The overall extraction time is less than 20 min, and the volume of the solvent is 1.5 times that of the extraction cell (34 mL). The recovery of 2-DCB from pork samples for this method is higher than 75%. The method allows detecting pork irradiated at low doses (0.5 kGy). The proposed method is considered an alternative to the Soxhlet extraction and Florisil chromatography method currently in use. © 2016 Elsevier Ltd


Wang M.,Beijing Research Center for Agricultural Standards and Testing | Wang M.,Risk Assessment Laboratory for Agro products Beijing | Jiang N.,Beijing Research Center for Agricultural Standards and Testing | Jiang N.,Risk Assessment Laboratory for Agro products Beijing | And 7 more authors.
Journal of Chromatography A | Year: 2016

A simple and rapid extraction procedure for the simultaneous determination of eight mycotoxins (Alternaria toxins, ochratoxin A, patulin, citrinin) in a variety of fruit matrices has been developed using ultra high performance liquid chromatography coupled to tandem mass spectrometry. The procedure involves a one-step cleanup using homemade solid phase extraction (SPE) cartridges. By comparative evaluation among six various adsorbents (C18, PSA, HLB, MCX, Silica, NH2), the combination of MCX and NH2 was found to provide the most effective cleanup, removing the greatest number of matrix interferences and also allowing the quantification of all analyzed mycotoxins in fruits. The optimized extraction conditions including acidified aqueous acetonitrile and an additional salt-out step using NaCl were employed before SPE cleanup. Method validation was performed by analyzing samples spiked at three levels (LOQ, 2 LOQ and 10 LOQ). Four fruits including apple, sweet cherry, tomato and orange fruits were selected, and accuracy (recovery%), precision (RSD%), limits of quantification (LOQ), linearity and matrix effect were evaluated during validation. Matrix-matched linearity with correlation coefficients ≥0.9921 was established in the range of 5-200ngmL-1 for patulin and 1-200ngmL-1 for other mycotoxins, respectively. Recoveries between 74.2% and 102.4% and relative standard deviations lower than 4.7% were obtained for all tested fruits. The matrix effect observed was low (≤±17%) in all three fruit matrixes with the exception of orange, for which strong ion suppression was observed for alternariol (25.3%), ochratoxin A (31.6%) and citrinin (40.3%). Therefore, matrix-matched calibration was used for a correct quantification in order to compensate for matrix effect. The limits of quantification (LOQ), ranging from 1 to 5μgkg-1 depending on mycotoxins type, were always lower than maximum permitted levels for every regulated mycotoxin by the current European legislation. © 2015 Elsevier B.V..


Xu L.,Beijing Academy of Agriculture and Forestry Sciences | Xu L.,Risk Assessment Laboratory for agro products Beijing | Li J.,CAS Beijing Institute of Geographic Sciences and Nature Resources Research
International Journal of Environmental Research and Public Health | Year: 2015

Environmental processes and biological community structures change along fluvial gradients within coastal river basins; the accumulation and associated risk of metal contamination would also be expected to change from upstream to downstream reaches. Speciation and degrees of contamination of metals in sediments from the upstream and downstream of river catchments of the southern Bohai Sea were investigated. The mean concentrations of Cu, Zn, Cr, Ni, Cd and Pb from upstream reaches were 82.6, 157, 63.6, 26.6, 0.18 and 24.9 mg/kg, respectively. The mean concentrations of Cu, Zn, Cr, Ni, Cd and Pb from downstream reaches were 38.0, 66.0, 38.9, 18.1, 0.16 and 24.0 mg/kg, respectively. Most of the Cu, Zn, Cr, Ni and Pb in sediments from both the upstream and downstream reaches was mainly associated with the residual fraction. However, Cd was preferentially bound to the exchangeable phase. A cluster analysis was used to study the degree of association between sites, and three distinct clusters were identified in both upstream and downstream sediments. A correlation analysis was conducted to determine the extent of association among metals and showed that metals in sediments from the upstream reaches have more affinity than those in the downstream area. Sediment quality guidelines were used to evaluate potential risks. The risks from Zn, Cr and Ni in the upstream reaches were higher than those from downstream reaches; however, the other three metals (Cu, Pb and Cd) showed opposite results. © 2015 by the authors; licensee MDPI, Basel, Switzerland.


Li C.,Beijing Academy of Agriculture and Forestry Sciences | Li C.,Risk Assessment Laboratory for Agro products Beijing | Chen J.,Beijing Academy of Agriculture and Forestry Sciences | Chen J.,Risk Assessment Laboratory for Agro products Beijing | And 11 more authors.
Science of the Total Environment | Year: 2015

The occurrence of 15 antibiotics in soil and manure samples from 11 large-scale greenhouse vegetable production (GVP) bases in Beijing, China was investigated. Results showed that the greenhouse soils were ubiquitously contaminated with antibiotics, and that antibiotic concentrations were significantly higher in greenhouses than in open field soils. The mean concentrations of four antibiotic classes decreased in the following order: tetracyclines (102. μg/kg). >. quinolones (86. μg/kg). >. sulfonamides (1.1. μg/kg). >. macrolides (0.62. μg/kg). This investigation also indicated that fertilization with manure and especially animal feces might be the primary source of antibiotics. A risk assessment based on the calculated risk quotients (RQs) demonstrated that oxytetracycline, chlortetracycline, norfloxacin, ciprofloxacin and enrofloxacin could pose a high risk to soil organisms. These results suggested that the ecological effects of antibiotic contamination in GVP bases and their potential adverse risks on human health need to be given special attention. © 2015 Elsevier B.V.


Yang L.,Beijing Research Center for Agricultural Standards and TestingBeijing | Yang L.,Beijing Academy of Agriculture and Forestry Sciences | Yang L.,Risk Assessment Laboratory for Agro products Beijing | Ligang P.,Beijing Research Center for Agricultural Standards and TestingBeijing | And 8 more authors.
International Journal of Agricultural and Biological Engineering | Year: 2015

The suitability evaluation model of the remediation technology of polluted farmland has been constructed by analytic hierarchy process. The evaluation index of remediation technology has been constructed based on the physical and chemical index of farmland, farming system and the characteristic of remediation technology. In addition, the potential risk factors such as the distance of the industrial and mining enterprises, and the economy factors such as repair cost and local economy level have all been considered to construct the suitability evaluation system of the remediation technology of polluted farmland. Shilou town in Beijing was selected for the testing site, with the pollution of cadmium, arsenic and organic matter pollution. Chemical passivation, phytoremediation and biological compost technology were used for restoring the polluted farmland. According to the suitability evaluation model, the phytoremediation technology is suitable for general pollution. And chemical passivation and biological compost technology are more suitable to restore the polluted farmland. The combined remediation technologies of chemical passivation and phytoremediation are more suitable. It can avoid the limit of the one technology, complement the defect for each other and receive more remediation effects. © 2015, Chinese Society of Agricultural Engineering.


Luan Y.,Beijing Academy of Agriculture and Forestry Sciences | Luan Y.,Risk Assessment Laboratory for Agro products Beijing | Chen J.,Beijing Academy of Agriculture and Forestry Sciences | Chen J.,Risk Assessment Laboratory for Agro products Beijing | And 9 more authors.
Toxins | Year: 2015

A label-free aptamer-based assay for the highly sensitive and specific detection of Ochratoxin A (OTA) was developed using a cationic polymer and gold nanoparticles (AuNPs). The OTA aptamer was used as a recognition element for the colorimetric detection of OTA based on the aggregation of AuNPs by the cationic polymer. By spectroscopic quantitative analysis, the colorimetric assay could detect OTA down to 0.009 ng/mL with high selectivity in the presence of other interfering toxins. This study offers a new alternative in visual detection methods that is rapid and sensitive for OTA detection. © 2015 by the authors; licensee MDPI, Basel, Switzerland.


Man Y.,Beijing Academy of Agriculture and Forestry Sciences | Man Y.,Risk Assessment Laboratory for Agro products Beijing | Man Y.,Beijing Municipal Key Laboratory of Agriculture Environment Monitoring | Liang G.,Beijing Academy of Agriculture and Forestry Sciences | And 8 more authors.
Chromatographia | Year: 2016

The genus Alternaria comprises ubiquitous pathogens and saprophytes. They can even grow at low temperature, so they are the main fungi responsible for the spoilage of various fruits, vegetables, grains and their products during long-distance transport and refrigerated storage. Alternaria mycotoxins are the secondary metabolite of the genus Alternaria. They can be divided into five main classes according to their chemical structures, including dibenzopyrone derivatives, tetramic acid derivatives, perylene derivatives, AAL toxins and miscellaneous structures. Alternaria mycotoxins are associated with many health effects because of their mutagenicity, teratogenicity and carcinogenicity, which can cause economic losses to agriculture and serious diseases in humans and animals. So far, there is still a lack of monitoring data on these contaminants of Alternaria mycotoxins. Moreover, there are still no statutory or guideline limits set for Alternaria mycotoxins in food and feed by regulatory authorities worldwide. Until now, many analytical methods have been developed for the detection and quantification of Alternaria mycotoxins. On the basis of briefly introducing the chemical structures and toxicities of Alternaria mycotoxins, this article provides an overview of the progress achieved in the detection techniques for Alternaria mycotoxins, focusing on the analytical methods of thin layer chromatography (TLC), gas chromatography (GC), gas chromatography-mass spectrometry (GC-MS), liquid chromatography (LC), liquid chromatography-mass spectrometry (LC-MS), enzyme-linked immunosorbent assay (ELISA) and so on. Finally, the problems of these analytical methods and future development trends are discussed. © 2016 Springer-Verlag Berlin Heidelberg

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