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Ẕefat, Israel

Chaturvedi C.S.,Central Institute of Fisheries Education | Singh R.K.,Research Wing | Panday A.K.,National Bureau of Fish Genetic Resources
Biochemical and Cellular Archives | Year: 2012

The endangered Ompok pabda (butterfish), weight range 80-130 gm, were successfully induced bred using ovaprim @ 1.0-1.5 ml/kg body weight of females and 0.5 ml/kg of males (single injection) under captivity. Breeding sets comprising males and females in ratio of 2:1, released in hapa under shower system, were bred after 12-16 hours of the drug administration without sacrificing the males. Latency period for breeding varied between 10-16 hours of the drug injection. Fertilization ranged from 60-80% and hatching success of 75% in flow-through hatchery system. Of the total 1,57,300 hatchlings reared during the induced breeding operations, 79,300 fry were produced with 50.4% survival while 23,580 seed of the non-air-breathing butterfish were raised with 29.8% survival. The observed successful induced breeding of Ompok pabda in all the sets and better larval survival in flow-through-hatchery pave the way for its mass seed production for conservation aquaculture and rehabilitation of this endangered species in the depleted water bodies.

Pikkel D.,Risk Management and Patient Safety Unit | Sharabi-Nov A.,Research Wing | Sharabi-Nov A.,Tel-Hai Academic College | Pikkel J.,Ziv Medical Center | Pikkel J.,Bar - Ilan University
International Journal of Risk and Safety in Medicine | Year: 2014

BACKGROUND: Wrong site confusions are among the most common mistakes in operations of twosome organs. PURPOSE: To examine the frequency of wrong sided confusions that could theoretically occur in various surgeries in the absence of preoperative verification. METHODS: Ten cataract surgeons, twelve orthopedic surgeons and 6 ENT surgeons participated in the study. The surgeons were asked to fill a questionnaire that included their demographic data, occupational habits and their approach to and handling of patients preoperatively. On the day of operation the surgeons were asked to recognize the side of the operation from the patient's name only. At the second stage of the study, surgeons were asked to recognize the side of the operation while standing a two meter distance from the patient's face. Surgeons' answers were compared to the actual operation side. Patients then underwent a full 'time out' procedure, which included side marking before the operation. RESULTS: Of a total of 67 ophthalmic patients, 52 orthopedic patients and 26 ENT patients the surgeons correctly identified the operated side in 111 (76.5%) by name and in 126 (87%) by looking at patients' faces. Wrong side identification correlated with the time lapsed from the last preoperative examination (p = 0.034). The number of cataract surgeries performed by the same surgeon (on the same day) also correlated to the number of wrong identifications (p = 0.001) in ophthalmology. Orthopedic surgeons were more accurate in identifying the operated site Surgeon seniority or age did not correlate to the number of wrong identifications. CONCLUSIONS: This study illustrates the high error that can result in the absence of side marking prior to cataract surgery, as well as in operations on other twosome organs. © 2014 - IOS Press and the authors.

Pikkel J.,Ziv Medical Center | Pikkel J.,Bar - Ilan University | Chassid O.,Ziv Medical Center | Sharabi-Nov A.,Research Wing | And 3 more authors.
Graefe's Archive for Clinical and Experimental Ophthalmology | Year: 2013

Background: To investigate a possible structural difference in the retina of hydroxychloroquine (Plaquenil)-treated patients as an explanation for the protective effect of this medication against age-related macular degeneration (AMD). Methods: In this retrospective study, we compared the mean thickness of the retinal outer band (consisting of the Bruch's membrane and retinal pigment epithelium layer), as measured by optical coherence tomography (OCT), of 54 eyes of 27 hydroxychloroquine-treated rheumatoid arthritis patients (study group), 40 eyes of 20 healthy similar aged individuals (control group I), and 22 eyes of 11 non-hydroxychloroquine-treated rheumatoid arthritis patients (control group II). Results: The mean thicknesses of the outer band of the retinal pigment epithelium layer was 60.4 ± 7.4, 43.3 ± 2.7, and 39.7 ± 3.6 μm for the study group, control group I, and control group II, respectively. P values for differences in mean thicknesses were < 0.0001 between the study group and each of the control groups, and 0.086 between the two control groups. Conclusion: Treatment with hydroxychloroquine was associated with increased thickness of the outer band of the retinal pigment epithelium layer. This finding may explain the protective effect of hydroxychloroquine against age-related macular degeneration (AMD). © 2013 Springer-Verlag Berlin Heidelberg.

Ranjan S.,Vellore Institute of Technology | Ranjan S.,Research Wing | Dasgupta N.,Vellore Institute of Technology | Rajendran B.,Vellore Institute of Technology | And 3 more authors.
Environmental Science and Pollution Research | Year: 2016

Titanium dioxide nanoparticles (TNPs) are widely used in the pharmaceutical and cosmetics industries. It is used for protection against UV exposure due to its light-scattering properties and high refractive index. Though TNPs are increasingly used, the synthesis of TNPs is tedious and time consuming; therefore, in the present study, microwave-assisted hybrid chemical approach was used for TNP synthesis. In the present study, we demonstrated that TNPs can be synthesized only in 2.5 h; however, the commonly used chemical approach using muffle furnace takes 5 h. The activity of TNP depends on the synthetic protocol; therefore, the present study also determined the effect of microwave-assisted hybrid chemical approach synthetic protocol on microbial and cytotoxicity. The results showed that TNP has the best antibacterial activity in decreasing order from Escherichia coli, Bacillus subtilis, and Staphylococcus aureus. The IC50 values of TNP for HCT116 and A549 were found to be 6.43 and 6.04 ppm, respectively. Cell death was also confirmed from trypan blue exclusion assay and membrane integrity loss was observed. Therefore, the study determines that the microwave-assisted hybrid chemical approach is time-saving; hence, this technique can be upgraded from lab scale to industrial scale via pilot plant scale. Moreover, it is necessary to find the mechanism of action at the molecular level to establish the reason for greater bacterial and cytotoxicological toxicity. [Figure not available: see fulltext.] © 2016, Springer-Verlag Berlin Heidelberg.

Dasgupta N.,Vellore Institute of Technology | Ranjan S.,Vellore Institute of Technology | Ranjan S.,Research Wing | Mundra S.,Vellore Institute of Technology | And 2 more authors.
International Journal of Food Properties | Year: 2016

The present study was carried out to fabricate the food grade Vitamin E acetate nanoemulsion using edible mustard oil and to evaluate its improved bioactivities. A food-grade Vitamin E acetate nanoemulsion was fabricated using the edible mustard oil and surfactant Tween-80. Flocculation was not observed for 15 days. The nanoemulsion was characterized for droplet morphology and size distribution using atomic force microscope and zetasizer, respectively. We observe a stable nanoemulsion of spherical morphology and a size distribution of 86.45 ± 3.61 nm. Further, the high-performance liquid chromatography method was used to determine the Vitamin E acetate concentration and encapsulation efficiency for the stable nanoemulsion. These nanoemulsions showed improved bioactivity, antioxidant, and antimicrobial activity and could be potentially used to increase the shelf life of fruit juice. Copyright © 2015 Taylor & Francis Group, LLC.

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