Research Unit UR04CNSTN01 Medical and Agricultural Applications of Nuclear Techniques

Sidi Bou Saïd, Tunisia

Research Unit UR04CNSTN01 Medical and Agricultural Applications of Nuclear Techniques

Sidi Bou Saïd, Tunisia
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Yahya M.,Research Unit UR04CNSTN01 Medical and Agricultural Applications of Nuclear Techniques | Yahya M.,Tunis el Manar University | Blanch A.R.,University of Barcelona | Meijer W.G.,University College Dublin | And 3 more authors.
Journal of Environmental Quality | Year: 2017

Microbial source tracking (MST) has been extensively used to detect the sources of fecal pollution in water. The inclusion of MST in water management strategies improves the ecological status of the ecosystem and human and animal health under interdisciplinary analysis in all aspects of health care for humans, animals, and the environment (One Health approach). In this study, the performance of MST markers targeting host-specific Bacteroidales (HF183 and Rum-2-Bac) and Bifidobacterium species (HMBif and CWBif) were evaluated in raw sewage collected from human, ruminant, swine, and poultry sources in Tunisia, Cyprus, Ireland, and Spain. In addition, the ratio between somatic coliphages and bacteriophages infecting Bacteroides GA17 (SOMCPH/GA17PH) was measured in Tunisia and Spain. The obtained results showed variability of the bacterial markers between the four countries, suggesting that their usefulness could be affected by several conditions (dietary habits, agricultural practices, and climatic conditions) that differ between countries. The Rum-2-Bac marker stood out as a valid MST tool, particularly in Ireland, whereas CWBif was the best option in Tunisia, Spain, and Cyprus. The human-specific HMBif marker showed good sensitivity and specificity in Tunisia, Spain, and Ireland, whereas HF183 showed a low specificity. However, HF183 was suggested as a good human marker in Ireland and Cyprus because of its higher concentration than HMBif. Regarding viral markers, the ratio of SOMCPH/GA17PH showed a clear discrimination between human and nonhuman samples. The combined use of molecular bacterial markers and the ratio of SOMCPH/GA17PH may improve the success of MST. © American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America. 5585 Guilford Rd., Madison, WI 53711 USA.


Mezhoud K.,Research Unit UR04CNSTN01 Medical and Agricultural Applications of Nuclear Techniques | Mezhoud K.,CNRS Laboratory of Communication Molecules and Adaptation of Microorganisms | Sakly A.,University of Sfax | Cheikh H.B.,Sina | And 2 more authors.
International Journal of Low Radiation | Year: 2014

Radiology is an essential technology in medicine and is used for organ diagnosis, radio-tracing and radiotherapy. The risks for the radiobiology workers have not been assessed sufficiently because measuring instruments fail to detect very low doses. This paper presents an investigation on the potential risks for radiobiology workers, due to the occupational exposure to low doses of irradiation. In this respect we used plasma samples from 16 subjects who were supposed to receive very low dose of irradiation in a longer time period. We used chromosomal aberrations, means of oxidative stress measurement and combined proteomics and bioinformatics in order to elucidate risks of such exposure. We found significant chromosome aberrations in lymphocytes and the increase of oxidative stress biomarkers in plasma. In addition, the proteomic analysis shows differentially regulated proteins from which three were verified by ELISA tests. This proteomic analysis picks out some interesting proteins that may belong to biomarkers panel of radiation exposure. Copyright © 2014 Inderscience Enterprises Ltd.


Hamden H.,Research Unit UR04CNSTN01 Medical and Agricultural Applications of Nuclear Techniques | Guerfali M.M.,Research Unit UR04CNSTN01 Medical and Agricultural Applications of Nuclear Techniques | Fadhl S.,Research Unit UR04CNSTN01 Medical and Agricultural Applications of Nuclear Techniques | Saidi M.,Research Unit UR04CNSTN01 Medical and Agricultural Applications of Nuclear Techniques | Chevrier C.,CNRS Research Institute of Insect Biology
Journal of Economic Entomology | Year: 2013

Successful mass rearing is crucial for sterile insect technique programs. It has been shown that the sterilizing process using gammaradiation results in damage to midgut tissue, cellular organelles, and gut microbiota of flies. This can be responsible for the inferiority of sterile males compared with wild males. A bacteria-enhanced diet could contribute to the improvement of the fly's fitness. We investigated ways of increasing the competitiveness of mass-reared Mediterranean fruit fly, Ceratitis capitata (Wiedemann) sterile males. We tested the hypothesis that the addition of beneficial bacteria to the larvae's diet would lead to a significant increase in their levels in the gut of the sterile adults and consequently improve their size and fitness. As expected, enriching the diet of mass-rearing Vienna-8 strain larvae with beneficial bacteria (Klebsiella pneumonia, Enterobacter spp., and Citrobacter freundii) resulted in increase in the number of Enterobacteriacae communities inhabiting the male's gut and a subsequent significant increase in the size of males and other morphometric traits and enhanced sexual performance of males at emergence. © 2013 Entomological Society of America.


Sghaier H.,Research Unit UR04CNSTN01 Medical and Agricultural Applications of Nuclear Techniques | Satoh K.,Japan Atomic Energy Agency | Narumi I.,Japan Atomic Energy Agency
Journal of Biomolecular Screening | Year: 2011

RecA is a highly conserved bacterial protein that plays crucial roles in many cellular processes and hence is a potential target in the chemotherapy of bacterial infections. An understanding of the functional similarity between RecA proteins from different bacterial species should yield further insights into the biochemistry of RecA protein, along with the potential for new approaches to facilitate the improvement of RecA-targeted drugs. In this technical note, the authors present an in silico method based on tri-oligonucleotide usage correlations (TOUC) to predict the functional similarity between two RecA orthologs. The TOUC values analyzed in this study are in good agreement with the available experimental results. This method should prove useful in guiding future experimental efforts aimed at furthering our understanding of the biochemistry of RecA proteins and subsequent development of new drugs that modulate RecA biological activities in bacteria. (Journal of Biomolecular Screening 2011;16:457-459) © 2011 Society for Laboratory Automation and Screening.


PubMed | Research Unit UR04CNSTN01 Medical and Agricultural Applications of Nuclear Techniques
Type: Journal Article | Journal: Journal of biomolecular screening | Year: 2011

RecA is a highly conserved bacterial protein that plays crucial roles in many cellular processes and hence is a potential target in the chemotherapy of bacterial infections. An understanding of the functional similarity between RecA proteins from different bacterial species should yield further insights into the biochemistry of RecA protein, along with the potential for new approaches to facilitate the improvement of RecA-targeted drugs. In this technical note, the authors present an in silico method based on tri-oligonucleotide usage correlations (TOUC) to predict the functional similarity between two RecA orthologs. The TOUC values analyzed in this study are in good agreement with the available experimental results. This method should prove useful in guiding future experimental efforts aimed at furthering our understanding of the biochemistry of RecA proteins and subsequent development of new drugs that modulate RecA biological activities in bacteria.

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