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Cordova-Galaviz Y.,Research Unit on Cell Differentiation and Cancer | Ledesma-Martinez E.,Research Unit on Cell Differentiation and Cancer | Aguiniga-Sanchez I.,Research Unit on Cell Differentiation and Cancer | Soldevila-Melgarejo G.,National Autonomous University of Mexico | And 3 more authors.
In Vivo | Year: 2014

Background: Acute myeloid leukaemia is a neoplastic disease of haematopoietic stem cells. Although there have been recent advances regarding its treatment, mortality remains high. Consequently, therapeutic alternatives continue to be explored. In the present report, we present evidence that sodium caseinate (CasNa), a salt of the principal protein in milk, may possess important anti-leukaemic properties. Materials and Methods: J774 leukaemia macrophage-like cells were cultured with CasNa and proliferation, viability and differentiation were evaluated. These cells were also inoculated into BALB/c mice as a model of leukemia. Results: We demonstrated that CasNa inhibits the in vitro proliferation and reduces viability of J774 cells, and leads to increased survival in vivo in a leukaemic mouse model. Conclusion: These data indicate that CasNa may be useful in leukaemia therapy. Source

Luviano A.,National Autonomous University of Mexico | Aguiniga-Sanchez I.,Research Unit on Cell Differentiation and Cancer | Demare P.,National Autonomous University of Mexico | Tiburcio R.,Research Unit on Cell Differentiation and Cancer | And 3 more authors.
Oncology Letters | Year: 2014

In the search for novel chemotherapeutic agents for cancer treatment, capsaicin has been shown to inhibit proliferation and induce apoptosis in various types of cancer cell line, including leukaemia cell lines. The capsaicin analogues, rinvanil and phenylacetylrinvanil (PhAR), share a binding affinity for vanilloid receptors and may have biological activities similar to capsaicin; however, their anticancer potential has not yet been reported. This study analyses the antineoplastic activities of rinvanil and PhAR in leukaemia versus normal cells. P388, J774 and WEHI-3 leukaemia cell lines, as well as mouse bone marrow mononuclear cells, were cultured with varying concentrations of rinvanil and PhAR. Following this, proliferation and apoptosis were determined by the sulforhodamine B (SRB) assay and DNA ladder. Cultured leukaemia cell lines and mouse bone marrow mononuclear cells demonstrated a dose-dependent inhibition of proliferation, while non-diseased cells were less sensitive to the cytotoxic effect of capsaicin, rinvanil and PhAR. Rinvanil and PhAR also induced apoptosis in leukaemia cell lines but not in bone marrow. Given the lower IC50 values for apoptosis induction in leukaemia cells compared with that of normal cells, PhAR is a promising selective anticancer agent. Source

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