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Alimi H.,Research unit of Macromolecular Biochemistry and Genetic
General physiology and biophysics | Year: 2013

Rhus tripartitum (sumac) is an Anacardiaceae tree with a wide phytotherapeutic application including the use of its roots in the management of gastric ulcer. In the present study the Rhus tripartitum root barks extract (RTE) was phytochemical studied, in vitro tested for their potential antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and reducing power assay and in vivo evaluated for its ability to prevent ethanol-induced gastric ulcer in rats. The RTE was rich in phenolics, flavonoids, tannins and polysaccharide contents and exhibited a low but not weak in vitro antioxidant activity when compared with (+)-catechin. Pre-treatment with RTE at oral doses 50, 200 and 400 mg/kg body weight was found to provide a dose-dependent protection against ethanol-induced ulcer by averting the deep ulcer lesions of the gastric epithelium, by reducing gastric juice and acid output, by enhancing gastric mucus production by preserving normal antioxidant enzymes activities, and inhibiting the lipid peroxidation. The antiulcerogenic activity of RTE might be due to a possible synergistic antioxidant and antisecretory effects. Source


Brahmi D.,Laboratory of Research on Biologically Compatible Compounds | Brahmi D.,Research unit of Macromolecular Biochemistry and Genetic | Ayed Y.,Laboratory of Research on Biologically Compatible Compounds | Bouaziz C.,Laboratory of Research on Biologically Compatible Compounds | And 4 more authors.
Journal of Medicinal Plant Research | Year: 2011

The cactus Opuntia ficus-indica is a xerophyte plant it has an increasing interest of nutritional and pharmacological power. In the present study we choose cactus cladode extract (CCE) to investigate its efficacy against benzo(a)pyrene (BAP) a widespread environmental genotoxin classified as probably carcinogenic to humans witch induced liver injury. Our results using balb/c mice clearly showed that BAP induced significant alterations in all tested oxidative stress markers the malondialdehyde level (MDA), the catalase activity and the expression of the heat shock proteins (Hsp 70) and (Hsp 27) which increased. In addition, it induces deoxyriboNucleic acid (DNA) fragmentation in liver and chromosomal aberrations in bone morrow cells. Moreover it increases the expression of anti apoptotic proteins bcl2 and decrease the expression of bax. The treatment of CCE after or before treatment with BAP showed a total reduction of BAP induced oxidative damage for all tested markers, it showed also an antigenotoxic effect compared to the group treated with BAP alone, CCE was able to restrict the effect of BAP by differential modulation of the expression of p53 which is increased and its associated genes such as bax and bcl2. We concluded that CCE was effective in the protection against BAP hazards. © 2011 Academic Journals. Source


Brahmi D.,Laboratory of Research on Biologically Compatible Compounds | Brahmi D.,Research unit of Macromolecular Biochemistry and Genetic | Ayed Y.,Laboratory of Research on Biologically Compatible Compounds | Hfaiedh M.,Research unit of Macromolecular Biochemistry and Genetic | And 6 more authors.
BMC Complementary and Alternative Medicine | Year: 2012

Background: Cis-Platinum (II) (cis-diammine dichloroplatinum; CDDP) is a potent antitumor compound widely used for the treatment of many malignancies. An important side-effect of CDDP is nephrotoxicity. The cytotoxic action of this drug is often thought to induce oxidative stress and be associated with its ability to bind DNA to form CDDP-DNA adducts and apoptosis in kidney cells. In this study, the protective effect of cactus cladode extract (CCE) against CDDP-induced oxidative stress and genotoxicity were investigated in mice. We also looked for levels of malondialdehyde (MDA), catalase activity, superoxide dismutase (SOD) activity, chromosome aberrations (CA) test, SOS Chromotest, expressions of p53, bax and bcl2 in kidney and we also analyzed several parameters of renal function markers toxicity such as serum biochemical analysis.Methods: Adult, healthy balb/c (20-25 g) male mice aged of 4-5 weeks were pre-treated by intraperitonial administration of CCE (50 mg/Kg.b.w) for 2 weeks. Control animals were treated 3 days a week for 4 weeks by intraperitonial administration of 100 μg/Kg.b.w CDDP. Animals which treated by CDDP and CCE were divided into two groups: the first group was administrated CCE 2 hours before each treatment with CDDP 3 days a week for 4 weeks. The second group was administrated without pre-treatment with CCE but this extract was administrated 24 hours after each treatment with CDDP 3 days a week for 4 weeks.Results: Our results showed that CDDP induced significant alterations in all tested oxidative stress markers. In addition it induced CA in bone morrow cells, increased the expression of pro-apoptotic proteins p53 and bax and decreased the expression of anti-apoptotic protein bcl2 in kidney. On the other hand, CDDP significantly increased the levels of urea and creatinine and decreased the levels of albumin and total protein.The treatment of CCE before or after treatment with CDDP showed, (i) a total reduction of CDDP induced oxidative damage for all tested markers, (ii) an anti-genotoxic effect resulting in an efficient prevention of chromosomal aberrations compared to the group treated with CDDP alone (iii) restriction of the effect of CDDP by differential modulation of the expression of p53 which is decreased as well as its associated genes such as bax and bcl2, (iiii) restriction of serums levels of creatinine, urea, albumin and total protein resuming its values towards near normal levels of control.Conclusion: We concluded that CCE is beneficial in CDDP-induced kidney dysfunction in mice via its anti-oxidant anti-genotoxic and anti-apoptotic properties against CDDP. © 2012 Brahmi et al.; licensee BioMed Central Ltd. Source


Brahmi D.,Laboratory of Research on Biologically Compatible Compounds | Brahmi D.,Research unit of Macromolecular Biochemistry and Genetic | Bouaziz C.,Laboratory of Research on Biologically Compatible Compounds | Ayed Y.,Laboratory of Research on Biologically Compatible Compounds | And 4 more authors.
Nutrition and Metabolism | Year: 2011

Background: Aflatoxin B1 (AFB1) is potent hepatotoxic and hepatocarcinogenic agent. In aflatoxicosis, oxidative stress is a common mechanism contributing to initiation and progression of hepatic damage. The aim of this work was to evaluate the hepatoprotective effect of cactus cladode extract (CCE) on aflatoxin B1-induced liver damage in mice by measuring malondialdehyde (MDA) level, the protein carbonyls generation and the heat shock proteins Hsp 70 and Hsp 27 expressions in liver. We also looked for an eventual protective effect against AFB1-induced genotoxicity as determined by chromosome aberrations test, SOS Chromotest and DNA fragmentation assay. We further evaluated the modulation of p53, bax and bcl2 protein expressions in liver. Methods. Adult, healthy balbC (20-25 g) male mice were pre-treated by intraperitonial administration of CCE (50 mg/Kg.b.w) for 2 weeks. Control animals were treated 3 days a week for 4 weeks by intraperitonial administration of 250 g/Kg.b.w AFB1. Animals treated by AFB1 and CCE were divided into two groups: the first group was administrated CCE 2 hours before each treatment with AFB1 3 days a week for 4 weeks. The second group was administrated without pre-treatment with CCE but this extract was administrated 24 hours after each treatment with AFB1 3 days a week for 4 weeks. Results: Our results clearly showed that AFB1 induced significant alterations in oxidative stress markers. In addition, it has a genotoxic potential and it increased the expression of pro apoptotic proteins p53 and bax and decreased the expression of bcl2. The treatment of CCE before or after treatment with AFB1, showed (i) a total reduction of AFB1 induced oxidative damage markers, (ii) an anti-genotoxic effect resulting in an efficient prevention of chromosomal aberrations and DNA fragmentation compared to the group treated with AFB1 alone (iii) restriction of the effect of AFB1 by differential modulation of the expression of p53 which decreased as well as its associated genes such as bax and bcl2. Conclusion: We concluded that CCE might have a hepatoprotective effect against aflatoxicosis in mice, probably acting by promoting the antioxidant defence systems. © 2011Brahmi et al; licensee BioMed Central Ltd. Source


Alimi H.,Research unit of Macromolecular Biochemistry and Genetic | Alimi H.,University of Carthage | Hfaeidh N.,University of Sfax | Mbarki S.,University of Sfax | And 3 more authors.
General Physiology and Biophysics | Year: 2012

The aimofour present study is to investigate the effect of Opuntia ficus indica f. inermisprickly pear juice (PPJ) against ethanol-induced liver injury in rats. Chronic ethanol administration (3 g/kg b.w.) during 90 days to Wistar rats, significantly (p < 0.01) increased the liver lipid and protein oxidation, reduced the glutathione content and the activities of liver antioxidant enzymes such as superoxide dismutase, catalase, glutathione peroxidase and conversely elevated the liver injury biochemical markers like aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, gamma glutamyl transferase, lactate dehydrogenase, cholesterol, triglycerides and caused a severe histopathologic injuries. Conversely pre-treatment of ethanol-fed rats with PPJ (20 and 40 ml/kg b.w., orally), interestingly reduced liver lipid and protein oxidation, histopathologic lesions and inhibited the alterations of antioxidant enzymes and the release of biochemical markers. The hepatoprotective effect of PPJ could be due to their capacity to end free radicals chain reactions or to enhance the endogenous antioxidants activities. Source

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