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Clapa D.,Research Station for Fruit Growing Cluj | Fira A.,Research Station for Fruit Growing Cluj | Simu M.,Research Station for Fruit Growing Cluj | Horga V.C.,Banat University of Agricultural Sciences and Veterinary Medicine, Timisoara
Scientific Papers of the Research Institute for Fruit Growing | Year: 2013

Our paper presents new aspects regarding the micropropagation of Gisela 5 cherry rootstock. In the multiplication stage, modified MS media supplemented with 0.3 and 0.5 mg/l BAP as well as two types of gelling agents were tested: 3 g/l Plant Agar and 50 g/l wheat starch. The media supplemented with 0.3 mg/l BAP and gelled with 3 g/l Plant Agar provided the highest proliferation rates (36.90 shoots/plantlet) and well-developed, high quality plantlets, whereas media gelled with starch also proved to be suitable but they caused deformations. In the in vitro rooting stage, MS media supplemented with 1 mg/l IBA provided 82.5 % rooting percentage and the plantlets were well developed. Ex vitro acclimatization of the plantlets rooted in vitro was successful in float hydroculture. Direct ex vitro rooting of shoots in floating perlite was also successful, with rooting pecentages exceeding 60 % but the plants were lesser developed as compared to the ones obtained by in vitro rooting and subsequent ex vitro acclimatization. Source

Clapa D.,Research Station for Fruit Growing Cluj | Fira Al.,Research Station for Fruit Growing Cluj | Vescan A.,Research Station for Fruit Growing Cluj | Zagrai I.,Research Station for Fruit Growing | And 3 more authors.
Acta Horticulturae | Year: 2013

In vitro propagation of plum cultivars 'Anna Späth', 'Carpatin', 'Dany', 'Ivan', 'Jubileu 50', 'Althanns Reneklode' and 'Stanley' was carried out in order to test the in vitro conservation and screenhouse maintenance of virus-free material. The initiation of the in vitro cultures was done in September 2009 from shoots belonging to virus free plants from the RSFG Bistrita. Murashige & Skoog 1962 (MS) + 0.7 mg/L 6-benzylaminopurine (BAP) medium was used both for culture initiation and for multiplication. As growth regulators, various concentrations of BAP, zeatin and gibberellic acid (GA3) were used. For rooting, MS basal medium was used and additional 1 mg/L 3-indolylbutyric acid (IBA) and ex vitro acclimation was done in hydroculture. Source

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