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Zarringhabaie G.E.,Natural Resources Research Center | Pirany N.,Shahrekord University | Javanmard A.,Agricultural Biotechnology Research Institute of Iran
African Journal of Biotechnology | Year: 2011

The objective of this study was the designing of a fast and reliable multiplex polymerase chain reaction (PCR) identification system for testing the pure and mixed species origin of meat samples. For conducting this research, different primers were designed for each species according to the conserved region of mitochondrial cytochrome b (Cytb) gene. The results revealed different specific amplified fragments of pure meat sources for buffalo, goat, cattle and sheep species. After mixing different portions of the mentioned meat sources, this method was able to trace less than 10% of the other species of meat in the mixture. Then, it can be concluded that this procedure is simple, cheap, rapid, and efficient, and so it can be used in the meat industry. © 2011 Academic Journals.

Ebrahimian E.,Islamic Azad University at Khoy | Bybordi A.,Natural Resources Research Center | Pasban Eslam B.,Natural Resources Research Center
Journal of Food, Agriculture and Environment | Year: 2010

In order to evaluate efficiency of zinc and iron microelements application methods on sunflower, an experiment was conducted in the Agricultural Research Station of Eastern Azerbaijan province in 2008. The experimental design was a RCBD with eight treatments (F 1 control, F2 iron, F3 zinc, F4 iron + zinc in the form of soil utility, F5 iron, F6 zinc, F7 iron+ zinc in the form of solution foliar application, and F8 iron + zinc in the form of soil utility and foliar application). Analysis of variance showed that there were significant differences among treatments on given traits, antioxidant enzymes activity, fatty acids percentage, plant height, seed weight to capitulum weight ratio, protein percentage, oil percentage, oil yield, 1000 seed weight, seed yield, nitrogen, phosphorus and potassium percentage of leaves, zinc and iron content of leaves and capitulum diameters. The highest seed yield, oil yield, oil percentage, 1000 seed weight, seed weight to capitulum weight ratio and protein percentage were obtained from the soil and foliar application of iron + zinc treatments (F8). Also, the highest amounts of nitrogen, phosphorus and potassium concentration in leaves were achieved from control treatment which was an indication of non-efficiency of iron and zinc on the absorption rate of these substances in the leaves. The correlation between effective traits on the seed yield, such as, capitulum diameter, number of seed rows in capitulum, seed weight to capitulum weight ratio and 1000-seed weight were positively significant. In general, foliar and soil application of zinc and iron had the highest efficiency in aspect of seed production. The comparison of the various methods of fertilization showed that foliar application was more effective than soil application. Also, micronutrient foliar application increased concentration of elements, especially zinc and iron. Antioxidant enzymes activity was different in response to treatments, also the highest palmitoleic, oleic and myristic acid were observed in F6 and F7 treatments.

Rhyan J.C.,National Wildlife Research Center | Nol P.,National Wildlife Research Center | Quance C.,National Veterinary Services Laboratories | Gertonson A.,Natural Resources Research Center | And 4 more authors.
Emerging Infectious Diseases | Year: 2013

Bovine brucellosis has been nearly eliminated from livestock in the United States. Bison and elk in the Greater Yellowstone Area remain reservoirs for the disease. During 1990-2002, no known cases occurred in Greater Yellowstone Area livestock. Since then, 17 transmission events from wildlife to livestock have been investigated.

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