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Luis-Pantoja M.,Research Institute on Tropical Fruit Crops | Ramos-Gonzalez P.L.,Research Institute on Tropical Fruit Crops | Naranjo M.,Research Institute on Tropical Fruit Crops | Hernandez-Rodriguez L.,Research Institute on Tropical Fruit Crops | And 3 more authors.
Journal of General Plant Pathology | Year: 2015

Papaya bunchy top (PBT) disease has been associated with Rickettsia-related proteobacterium. To study the occurrence of this proteobacterium in Cuba, during 2012, we collected 264 samples from asymptomatic and symptomatic papaya trees in three regions of Cuba. Symptomatic tissue evaluated by transmission electron microscopy revealed Rickettsia-like morphology. PCR using rickettsial sdhA-specific primers amplified a single band with expected size from 95 % of symptomatic plants. Phylogeny confirmed the presence of Rickettsia-related proteobacterium in plant showing PBT disease. This work confirms Rickettsia-related proteobacterium is a generalized pathogen in papaya plant affected by bunchy top disease in Cuba. © 2014, The Phytopathological Society of Japan and Springer Japan.


Hernandez-Rodriguez L.,Research Institute on Tropical Fruit Crops | Ramos-Gonzalez P.L.,Research Institute on Tropical Fruit Crops | Garcia-Garcia G.,Research Institute on Tropical Fruit Crops | Zamora V.,Research Institute on Tropical Fruit Crops | And 4 more authors.
Crop Protection | Year: 2014

Several species of ampeloviruses and badnaviruses infect pineapple plants around the world. Pineapple mealybug wilt-associated ampeloviruses have been associated with mealybug wilt of pineapple (MWP), the major viral disease threatening this crop. Conversely, infection by the badnaviruses Pineapple bacilliform comosus virus (PBCOV) and Pineapple bacilliform erectifolius virus (PBERV) is asymptomatic. To investigate the status of infection of the pineapple crop in Cuba, a diagnostic survey was developed in commercial areas during the period 2009-2012. Incidence of MWP disease was found in up to 100% of the plants in some fields of Central and Eastern regions of the island. Molecular assays revealed the presence of PMWaV-1 for the first time in the Caribbean basin and PMWaV-2, PMWaV-3, either as mixed infections or in combination with PBCOV throughout the country. Furthermore, they revealed for the first time the presence of PMWaV-2 in Bromelia pinguin L., a plant commonly used in Cuba as hedgerow. Sequence analysis of partial heat shock protein 70h and complete coat protein gene of Cuban isolates of PMWaV-1, -2 and -3 showed nucleotide identities above 97% with cognate sequences of viruses isolated from other countries. This work discloses the presence of a complex of viruses associated with the pineapple crop in Cuba, highlights the potential role of B.pinguin in the PMWaV-mealybug-pineapple pathosystem and makes available diagnostic tools for the detection of viruses affecting pineapple for a seed certified production system in Cuba. © 2014 Elsevier Ltd.

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