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Katula-Debreceni D.,Szent Istvan University | Szoke A.,Szent Istvan University | Lencses A.K.,Szent Istvan University | Kiss E.,Szent Istvan University | And 4 more authors.
Acta Horticulturae | Year: 2014

Gene pyramiding is a potential method to breed new grape cultivars with durable disease resistance. The use of resistance gene-linked DNA markers considerably reduces the volume of breeding experiments, since it makes possible the identification of progeny seedlings that have inherited the desired gene shortly after germination, and permits the reduction of population size. Saturating the grape genome with molecular markers, the construction of genetic linkage maps and the recent publication of the Vitis vinifera genome sequence allow for the direct selection the desired genotype. To combine powdery and downy mildew resistance genes Kozma et al. (Research Institute of Viticulture and Enology, Pécs) made the following crosses: 'BC4' ('VRH 3082-1-42') × V. vinifera 'Kishmish vatkana', 'BC4' × V. vinifera 'Kishmish moldavskiy', V. vinifera 'Génuai zamatos' × V. vinifera 'Kishmish vatkana', ('Lasta' × V. vinifera 'Dzhandzhal kara') × (V. vinifera 'Katta kurgán' × V. vinifera 'Perlette'). Our aim was to select individuals containing powdery (PM) and/or downy mildew (DM) major resistance genes of different origin (Muscadinia rotundifolia-RUN1, RPV1, V. vinifera-REN1, and PM and DM QTLs of Seyve-Villard) with SSR markers. We compared the symptomless progenies derived from the cross 'BC4' × 'Kishmish vatkana' and ('Lasta' × 'Dzhandzhal kara') × ('Katta kurgán' × 'Perlette') using resistance linked markers. We observed that alleles of SSR markers linked to the PM resistance gene REN1 in the linkage group 13 are the same, so the PM resistance genes of 'Kishmish vatkana' and 'Dzhandzhal kara' appear identical.


Sak M.,Research Institute of Viticulture and Enology | Sak M.,Slovak University of Technology in Bratislava | Dokupilova I.,Research Institute of Viticulture and Enology | Dokupilova I.,Slovak University of Technology in Bratislava | And 8 more authors.
Nova Biotechnologica et Chimica | Year: 2014

The in vitro cell cultures of Vitis vinifera L. cv. St. Laurent were treated with two elicitors - synthetic methyl jasmonate and natural, prepared from grapevine plant infected with the Phaeomoniella chlamydospora, the agent causing the Esca disease of grapevine. Efficiency of phenolic compounds production after elicitation of cell culture was analysed immediately after treatment (15 min, 30 min, 60 min) and later (after 24, 48, and 72 hours). The cell growth and content of phenolic compounds (+)-catechin, (-)-epicatechin, p-coumaric acid, syringaldehyde, rutin, vanillic acid, and trans-resveratrol were analysed in cultivated cells as well as in cultivation medium. Pch-treatment increased production of total polyphenols the most significantly 15 min after the elicitation and in optimal time was 2.86 times higher than in nonelicited culture and 1.44 times higher than in MeJa induced cell culture. © University of SS. Cyril and Methodius.


Moreira F.M.,Instituto Agrario San Michele allAdige Research and Innovation Center | Madini A.,Instituto Agrario San Michele allAdige Research and Innovation Center | Madini A.,Resnova S.r.l. | Marino R.,Instituto Agrario San Michele allAdige Research and Innovation Center | And 6 more authors.
Tree Genetics and Genomes | Year: 2011

Two populations (Pop) segregating quantitatively for resistance to downy mildew (DM), caused by Plasmopara viticola, were used to construct genetic maps and to carry out quantitative trait locus (QTL) analysis. Pop1 comprised of 174 F1 individuals from a cross of 'Moscato Bianco', a susceptible Vitis vinifera cultivar, and a resistant individual of Vitis riparia. Pop2 consisted of 94 progeny from a cross of two interspecific hybrids, 'VRH3082 1-42' and 'SK77 5/3', with resistance traits inherited from Vitis rotundifolia and Vitis amurensis, respectively. Resistance of progeny was measured in field and greenhouse conditions by visual evaluation of disease symptoms on leaves. Linkage maps of 1037.2 and 651 cM were built essentially with simple sequence repeat markers and were enriched with gene-derived single-strand conformational polymorphism and single-nucleotide polymorphism markers. Simple interval mapping and Kruskall-Wallis analysis detected a stable QTL involved in field resistance to DM on linkage group (LG) 7 of the Pop1 integrated map co-localized with a putative Caffeoyl-CoA O-methyltransferase-derived marker. Additional QTLs were detected on LGs 8, 12 and 17. We were able to identify genetic factors correlated with resistance to P. viticola with lower statistical significance on LGs 1, 6 and 7 of the Pop2 map. Finally, no common QTLs were found between the two crosses analyzed. A search of the grapevine genome sequence revealed either homologues to non-host-, host- or defense-signalling genes within the QTL intervals. These positional candidate genes may provide new information about chromosomal regions hosting phenotypic loci. © 2010 Springer-Verlag.


Tolgyessy P.,National Water Research Institute | Hrivnak J.,Expertise and Analytical Services | Hrivnak J.,Research Institute of Viticulture and Enology | Kral'Ovicova E.,Regional Authority of Public Health Service | Barlokova D.,Slovak University of Technology in Bratislava
Chromatographia | Year: 2010

An improvement of the on-column injection system for the use of large diameter inside needle capillary adsorption trap devices is presented. A modified glass liner in combination with a gas tight glass fitting, used in the conventional split/splitless gas chromatographic inlet, enables to keep impurities desorbing from the outside of the needle trap and septum from being injected into the analytical GC column. The performance of the system was verified by headspace analysis of model and real water samples for benzene, toluene, ethylbenzene and xylenes. The analytical method shows good linearity, repeatability, sensitivity and advantages such as simplicity, low cost and feasibility. © 2010 Vieweg+Teubner Verlag | Springer Fachmedien Wiesbaden GmbH.


Sak M.,Slovak University of Technology in Bratislava | Sak M.,Research Institute of Viticulture and Enology | Dokupilova I.,Research Institute of Viticulture and Enology | Sajbidor J.,Slovak University of Technology in Bratislava
Chemicke Listy | Year: 2015

The main aim of this study is to monitor the impact of biotic elicitor on resveratrol 3-β-D-glucoside production in cell culture of Vitis vinifera cv. Váh. Suspension cultures were treated with different concentrations of fungal elicitor with the aspect on polysaccharide components. The highest concentration of resveratrol 3-β-D-glucoside 236 mg g-1 (dry weight of plant cells) was determined in suspension culture on sixth day of cultivation after treatment with fungal elicitor with polysaccharide total content 60 mg (carbohydrate equivalent)/L. This concentration was 5, 44 times more than in the control suspension culture. © 2015, Czech Society of Chemical Engineering. All rights reserved.


Repka V.,Research Institute of Viticulture and Enology | Carna M.,Slovak Academy of Sciences | Carna M.,Slovak University of Technology in Bratislava
Agriculture | Year: 2012

In an attempt to identify novel and apoptosis/pathogen-regulated microRNAs (miRNAs) and small interfering RNAs, we performed a robust microarray screening of small RNA population from Vitis vinifera L. cv. Limberger cell suspension exposed to apoptosis activators (e.g. methyl jasmonate) or elicitors (botrycin and cinerein) derived from necrotrophic fungus Botrytis cinerea Pers. et Fries. Using a microarray expression profiling approach, we identified 22 miRNAs. We found that a majority of these miRNAs were predicted to target stress/defense-related genes of plants. Of the 22 V. vinifera miRNAs, 11 have sequence conservation in Arabidopsis thaliana but exhibited species-specific developmental and/or stress/defense-related expression patterns. Ten of the miRNAs are highly conserved in other plant species, suggesting that even conserved miRNAs may have different regulatory roles in various species. Our results show that these grapevine miRNAs can be also induced by various apoptosis inducers. Fifty-one potential targets were predicted to the newly identified miRNAs based on sequence complementarity. In addition to miRNAs, we identified 102 other novel endogenous small RNAs in Vitis, indicating that a large number of miRNAs and other small regulatory RNAs are encoded by the Vitis vinifera genome.

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