Research Institute of science and New Technology

Mashhad, Iran

Research Institute of science and New Technology

Mashhad, Iran
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Abnous K.,Mashhad University of Medical Sciences | Danesh N.M.,Mashhad University of Medical Sciences | Danesh N.M.,Research Institute of science and New Technology | Ramezani M.,Mashhad University of Medical Sciences | And 3 more authors.
Nanomedicine: Nanotechnology, Biology, and Medicine | Year: 2017

In this study, a novel chemotherapy drug-free DNA nanocomplex composed of three therapeutic aptamers (IDA, AS1411 and apMNK2F) was designed for treatment of cancer cells. For MTT assay, PC-3 and 4T1 cells (target cells) and CHO cells (nontarget cells) were treated with apMNK2F-AS1411-IDA complex (DNA nanocomplex), as well as AS1411, IDA and apMNK2F alone. Internalization of apMNK2F-AS1411-IDA complex was analyzed by fluorescence imaging and flow cytometry analysis. In the last step, the presented DNA nanocomplex was applied for prohibition of tumor growth in vivo. The results of internalization assay verified that the developed apMNK2F-AS1411-IDA complex was remarkably internalized into PC-3 and 4T1 cells, but not into CHO cells. The results of internalization assay was confirmed by MTT assay. apMNK2F-AS1411-IDA complex was more cytotoxic in PC-3 and 4T1 cells (target) and less cytotoxic in CHO cells (nontarget). Also, the DNA nanocomplex could effectively suppress the growth of tumors in vivo. © 2017 Elsevier Inc.


Taghdisi S.M.,Mashhad University of Medical Sciences | Danesh N.M.,Mashhad University of Medical Sciences | Danesh N.M.,Research Institute of science and New Technology | Ramezani M.,Mashhad University of Medical Sciences | And 2 more authors.
Microchimica Acta | Year: 2017

The authors describe an electrochemical aptasensing platform for the determination of Pb(II) ions. The assay is based on the use of gold nanoparticles (AuNPs) (that facilitate electron transfer), the enzyme exonuclease III (Exo III; assisting signal amplification), and a hairpin structured complementary strand of an aptamer that prevents binding of AuNPs to the surface of the sensor electrode in the presence of Pb(II). In the absence of Pb(II), AuNPs attach to the surface of the gold electrode, and this leads to a strong voltammetric signal. In the presence of Pb(II), however, the AuNPs do not bind to the surface of the electrode because of the hairpin structure of the complementary strand. As a result, the peak current (typically measured at 0.17 V vs. silver reference electrode) remains weak. The method has a 149 pM detection limit. It was applied to the analysis of spiked tap water and human serum samples and gave recoveries between 92 and 105.25%. [Figure not available: see fulltext.] © 2017 Springer-Verlag Wien


Danesh N.M.,Mashhad University of Medical Sciences | Danesh N.M.,Research Institute of science and New Technology | Lavaee P.,Ferdowsi University of Mashhad | Lavaee P.,Academic Center For Education | And 3 more authors.
International Journal of Pharmaceutics | Year: 2015

Abstract Clinical administration of daunorubicin (Dau) in treatment of leukemia has been limited by its cardiotoxicity. Targeted delivery of chemotherapy drugs could reduce their side effects and increase the therapeutic efficacy of these drugs. Biocompatibility and large surface area of gold nanoparticles (AuNPs) make these nanoparticles great candidates for biomedical applications. In this study sgc8c aptamer (Apt)-Dau-AuNPs complex was designed and evaluated for treatment of Molt-4 cells (human acute lymphoblastic leukemia T-cell, target). Apt-Dau-AuNPs complex formation was analyzed by fluorometric analysis and gel retardation assay. Dau release profiles from the complex were evaluated in pHs 5.5 and 7.4. For cytotoxic studies (MTT assay) U266 (B lymphocyte human myeloma, nontarget) and Molt-4 cells (target) were treated with Dau Apt-Dau conjugate and Apt-Dau-AuNPs complex. Internalization was monitored by flow cytometry and confocal imaging. 12 μM Dau was efficiently loaded onto 1 mL of Apt-modified AuNPs. Dau was released from the complex in a pH-dependent manner (higher rate of release at pH 5.5). The results of flow cytometry analysis and confocal imaging showed that the complex was effectively internalized into Molt-4 cells, but not into U266 cells. The results of MTT assay also confirmed the internalization data. Apt-Dau-AuNPs complex was less cytotoxic in U266 cells compared to Dau alone and even Apt-Dau conjugate. The complex was more cytotoxic in target cells in comparison with Dau alone and even Apt-Dau conjugate. In conclusion, Apt-Dau-AuNPs complex was able to selectively target Molt-4 cells. Another advantage of this system was pH-dependent release of drug from the complex. Furthermore, this complex has characteristics which make it ideal for clinical use. © 2015 Elsevier B.V.


Taghdisi S.M.,Mashhad University of Medical Sciences | Danesh N.M.,Mashhad University of Medical Sciences | Danesh N.M.,Research Institute of science and New Technology | Ramezani M.,Mashhad University of Medical Sciences | And 2 more authors.
Analytica Chimica Acta | Year: 2017

It is great significance to identify interferon-gamma (IFN-γ), as a biological marker for diagnosis of latent tuberculosis, in serum samples. In this paper, a novel fluorescent aptasensor was fabricated and applied for sensitive and specific detection of IFN-γ. This biosensor was based on hairpin structure of oligonucleotide, single-stranded DNA-binding protein (SSB), copper nanoparticles (CuNPs) and silica nanoparticles coated with streptavidin (SNPs-Streptavidin). The presences of double-stranded DNA (dsDNA) region and poly thymine (T) in the hairpin structure of the oligonucleotide, SSB and SNPs-streptavidin caused IFN-γ determination with high selectivity and sensitivity. Upon addition of IFN-γ, the hairpin structure of the oligonucleotide was disassembled and therefore, poly T strand interacted with SSB and a weak fluorescence signal was obtained. Without introduction of IFN-γ, the hairpin structure of oligonucleotide was preserved and fluorescent CuNPs were formed on the stem region of hairpin structure of oligonucleotide, resulting in strong fluorescence intensity. Under optimal conditions, concentration as low as 1 pg/mL IFN-γ could be detected, with a linear range between 10 pg/mL and 4 ng/mL. The presented method was further performed for detection of IFN-γ in the spiked human serum samples and the recoveries were 92.52%-98.32%, showing the great potential of the proposed analytical method in biomedical analysis. © 2017 Elsevier B.V.


Ramezani M.,Mashhad University of Medical Sciences | Mohammad Danesh N.,Mashhad University of Medical Sciences | Mohammad Danesh N.,Research Institute of science and New Technology | Lavaee P.,Academic Center For Education | And 3 more authors.
Biosensors and Bioelectronics | Year: 2015

Detection methods of antibiotic residues in blood serum and animal derived foods are of great interest. In this study a colorimetric aptasensor was designed for sensitive, selective and fast detection of tetracycline based on triple-helix molecular switch (THMS) and gold nanoparticles (AuNPs). As a biosensor, THMS shows distinct advantages including high stability, sensitivity and preserving the selectivity and affinity of the original aptamer. In the absence of tetracycline, THMS is stable, leading to the aggregation of AuNPs by salt and an obvious color change from red to blue. In the presence of tetracycline, aptamer binds to its target, signal transduction probe (STP) leaves the THMS and adsorbs on the surface of AuNPs. So the well-dispersed AuNPs remain stable against salt-induced aggregation with a red color. The presented aptasensor showed high selectivity toward tetracyclines with a limit of detection as low as 266. pM for tetracycline. The designed aptasensor was successfully applied to detect tetracycline in serum and milk. © 2015 Elsevier B.V.


Taghdisi S.M.,Mashhad University of Medical Sciences | Danesh N.M.,Mashhad University of Medical Sciences | Danesh N.M.,Research Institute of science and New Technology | Beheshti H.R.,Testa Quality Control | And 2 more authors.
Nanoscale | Year: 2016

Analytical approaches for the detection and quantitation of ochratoxin A (OTA) in blood serum and food products are high in demand. In this study, a fluorescent aptamer-based sensor (aptasensor) is developed for the selective and sensitive detection of OTA, based on a complementary strand of aptamer (CS) and two types of nanoparticles, gold nanoparticles (AuNPs) and silica nanoparticles (SNPs) coated with streptavidin. The fabricated aptasensor inherits the characteristics of SNPs, as enhancers of fluorescence intensity; AuNPs, such as large surface area and unique optical properties; and high affinity of the aptamer toward its target compared to its CS. In the absence of OTA, no FAM and biotin-labeled CS is in the environment of the SNPs coated with streptavidin, which leads to no fluorescence emission. In the presence of the target, an FAM and biotin-labeled CS-SNPs coated with streptavidin conjugate is formed, thus resulting in a very strong fluorescence emission. The designed fluorescent aptasensor exhibits high selectivity toward OTA with a limit of detection (LOD) as low as 0.098 nM. Furthermore, the fabricated aptasensor was successfully applied for the detection of OTA in grape juice and serum with LODs of 0.113 and 0.152 nM, respectively. © 2016 The Royal Society of Chemistry.


Danesh N.M.,Mashhad University of Medical Sciences | Danesh N.M.,Research Institute of science and New Technology | Ramezani M.,Mashhad University of Medical Sciences | Emrani A.S.,Mashhad University of Medical Sciences | And 2 more authors.
Biosensors and Bioelectronics | Year: 2016

Detection and quantitation of antibiotic residues in blood serum and animal foodstuffs are of great significance. In this study, an electrochemical aptasensor was developed for sensitive and selective detection of streptomycin, based on exonuclease I (Exo I), complimentary strand of aptamer (CS), Archshape structure of aptamer (Apt)-CS conjugate and gold electrode. The designed aptasensor inherits characteristics of gold including large surface area and high electrochemical conductivity, as well as high sensitivity and selectivity of aptamer toward its target, property of Arch-shape structure of Apt-CS conjugate to act as a gate and barrier for the access of redox probe to the surface of electrode and the function of Exo I as an enzyme which selectively digests the 3'-end of single stranded DNA (ssDNA). In the absence of streptomycin the gate remains closed. Thus, the electrochemical signal is weak. Upon addition of streptomycin, the Apt leaves the CS and binds to streptomycin and the Arch-shape structure is disassembled. Then, Exo I addition leads to a strong electrochemical signal. The designed electrochemical aptasensor exhibited high selectivity toward streptomycin with a limit of detection (LOD) as low as 11.4 nM. Moreover, the developed electrochemical aptasensor was successfully used to detect streptomycin in milk and serum with LODs of 14.1 and 15.3 nM, respectively. © 2015 Elsevier B.V. Allrights reserved.


Taghdisi S.M.,Mashhad University of Medical Sciences | Danesh N.M.,Mashhad University of Medical Sciences | Danesh N.M.,Research Institute of science and New Technology | Ramezani M.,Mashhad University of Medical Sciences | Abnous K.,Mashhad University of Medical Sciences
Biosensors and Bioelectronics | Year: 2016

Analytical techniques for detection and quantitation of tetracyclines in food products are greatly in demand. In this study, a novel electrochemical aptasensor was designed for ultrasensitive and selective detection of tetracyclines, based on M-shape structure of aptamer (Apt)-complementary strands of aptamer (CSs) complex, exonuclease I (Exo I) and gold electrode. The aptasensor was developed to make a noticeable electrochemical difference in the absence and presence of tetracycline. In the absence of tetracycline, the M-shape structure, which acts as a gate and barrier for the access of redox probe to the surface of gold electrode remains intact, leading to a weak electrochemical signal. Upon addition of tetracycline, Apt leaves CSs, resulting in disassembly of M-shape structure and following the addition of Exo I, a strong electrochemical signal was observed. The developed analytical assay indicated high selectivity toward tetracycline with a limit of detection (LOD) as low as 450 pM. Moreover, the designed aptasensor was effectively used for the detection of tetracycline in milk and serum samples with LODs of 740 and 710 pM, respectively. © 2016 Elsevier B.V.


Taghdisi S.M.,Mashhad University of Medical Sciences | Danesh N.M.,Mashhad University of Medical Sciences | Danesh N.M.,Research Institute of science and New Technology | Ramezani M.,Mashhad University of Medical Sciences | And 2 more authors.
Biosensors and Bioelectronics | Year: 2016

Monitoring of myoglobin (Mb) in human blood serum is highly in demand for early diagnosis of acute myocardial infarction (AMI). Here, a novel electrochemical aptasensor was developed for ultrasensitive and selective detection of Mb, based on Y-shape structure of dual-aptamer (DApt)-complementary strand of aptamer (CS) conjugate, gold electrode and exonuclease I (Exo I). The designed aptasensor obtains features of gold, such as high electrochemical conductivity and large surface area, property of Y-shape structure of DApt-CS conjugate to function as a gate and obstacle for the access of redox probe to the surface of electrode, as well as high specificity and sensitivity of aptamer toward its target and Exo I as an enzyme which specifically degrades the 3'-end of single-stranded DNA (ssDNA). In the absence of Mb, the Y-shape structure remains intact. So, a weak electrochemical signal is observed. Upon addition of target, the DApt leave the CS and bind to Mb, leading to disassembly of Y-shape structure and following the addition of Exo I, a strong electrochemical signal could be recorded. The fabricated aptasensor showed high selectivity toward Mb with a limit of detection (LOD) as low as 27 pM. Besides, the developed aptasensor was effectively applied to detect Mb in human serum. © 2016 Elsevier B.V.


Abnous K.,Mashhad University of Medical Sciences | Danesh N.M.,Mashhad University of Medical Sciences | Danesh N.M.,Research Institute of science and New Technology | Ramezani M.,Mashhad University of Medical Sciences | And 2 more authors.
Sensors and Actuators, B: Chemical | Year: 2016

Cocaine is one of the most commonly abused stimulant which could affect the central nervous system. In this work, an electrochemical aptasensor was designed for sensitive and selective detection of cocaine, based on complimentary strands of aptamer (CSs), H-shape structure of Aptamer (Apt)-CSs conjugate and gold electrode. This aptasensor inherits properties of gold such as high electrochemical conductivity and large surface area, as well as high selectivity and sensitivity of aptamer toward its target and property of H-shape structure of Apt-CSs conjugate to act as a gate for the access of redox probe to the surface of electrode. In the absence of cocaine, the gate is closed, so that the electrochemical signal is weak. In the presence of cocaine, aptamer binds to target, leaves the surface of electrode and the gate is opened, leading to a strong electrochemical signal. The fabricated electrochemical aptasensor exhibited good selectivity toward cocaine with a limit of detection (LOD) as low as 0.228 nM. Furthermore, the designed aptasensor was successfully used to detect cocaine in serum with a LOD as low as 0.273 nM. © 2015 Elsevier B.V. All rights reserved.

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