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Roshchupkin D.I.,Russian National Research Medical University | Murina M.A.,Research Institute of Physicochemical Medicine | Sergienko V.I.,Research Institute of Physicochemical Medicine
Biophysics | Year: 2011

The quantum mechanics computation of the reactivities of chloramine derivatives of amino acids and taurine has been accomplished. A pair of computational indices that reflect a predisposition of alpha amino acid chloramines to chemical decay have been revealed. One of the indices was the dihedral angle for the chain of four atoms: carbons at beta- and alpha-positions, carbon of the carboxyl group, and carbonyl oxygen. The second index was the sum of partial charges for three or two carbon atoms in the chain. The amino acid chloramines with high values of the indices showed enhanced stability. Partial charges for active chlorine in known chloramines having different structures have been computed. The charges correlate with the rate constants of the reaction between chloramines and the thiol group of reduced glutathione. New derivatives of taurine chloramines have been constructed via the introduction of different substituents into the chloramine part. Among them, the amidoderivatives had the greatest charges of active chlorine (0. 19-0. 23). It was found in the study of the reactions of N-acetyl-N-chlorotaurine and N-propyonyl-N-chlorotaurine with amino acids and peptides possessing the thiol, thioester, or disulphide groups that the amidoderivatives manifested the thiol chemoselectivity. N-acetyl-N-chlorotaurine and N-propionyl-N-chlorotaurine suppress the aggregation activity of blood platelets under their activation by the agonists ADP and collagen. It is not excluded that the amidoderivatives studied prevent platelet aggregation by a modification of the critical thiol group in the purine receptor P2Y12. © 2011 Pleiades Publishing, Ltd.


Kozlov A.A.,Moscow State University | Abdullaev S.D.,Moscow State University | Flid V.R.,Moscow State University | Gusev S.A.,Research Institute of Physicochemical Medicine
Russian Journal of Physical Chemistry A | Year: 2016

An algorithm for assessing the quality of the packing of two-dimensional ordered structures, prepared using polymer microspheres 20 μm in diameter on a water surface, is proposed. An analysis is performed on the basis of optical microscopy images. The area of the largest ordered microsphere domain in an image is used as the quality criterion. The algorithm simplifies analysis of the ordered structures. © 2016, Pleiades Publishing, Ltd.


Zavalova L.L.,RAS Shemyakin Ovchinnikov Institute of Bioorganic Chemistry | Lazarev V.N.,Research Institute of Physicochemical Medicine | Levitsky S.A.,Research Institute of Physicochemical Medicine | Yudina T.G.,Moscow State University | Baskova I.P.,Moscow State University
Biochemistry (Moscow) | Year: 2010

Preparation and purification of a recombinant protein are described along with characteristics of its specific (for ε-(γ-Glu)-Lys and D-dimer substrates) and nonspecific (for L-γ-Glu-pNA) isopeptidase activities; the absence of peptidase function for α-(α-Glu)-Lys substrate is noted. It is shown that the protein exhibits muramidase (cell walls of Micrococcus lysodeikticus) and specific glycosidase activities. The latter was determined towards the fluorogenic substrate 4-methylum-belliferyl-tetra-N-acetyl-β- chitotetraoxide. Antimicrobial activity of recombinant destabilase-lysozyme protein (recDest-Lys) and its 11-membered amphipathic peptide was revealed towards cells of the strict anaerobic Archaean Methanosarcina barkeri, whose cell walls contain no murein. Possible mechanisms of the effect of recDest-Lys on these cells are discussed. © 2010 Pleiades Publishing, Ltd.


Stovbun S.V.,RAS Semenov Institute of Chemical Physics | Berlin A.A.,RAS Semenov Institute of Chemical Physics | Mikhailov A.I.,RAS Semenov Institute of Chemical Physics | Sergienko V.I.,Research Institute of Physicochemical Medicine | And 3 more authors.
Nanotechnologies in Russia | Year: 2012

A high-molecular-weight plant polysaccharide belonging to the hexose glycoside (HG) class and composed of rhamnose (2-10%), arabinose (3-15%), glucose (10-67%), galactose (2-27%), xylose (0. 1-3%), and mannose (0. 1-5%) monosaccharides, as well as uronic acids (2-5%), had been isolated previously. It has high antiviral activity, as is proven by experimental models using hazardous and extremely dangerous viral infections such as herpes, cytomegalovirus, influenza, encephalitis, measles, rabies, and hepatitis C. The antiviral drug Panavir developed on its basis has an extremely low toxicity (LD 50 = 3000) and is an antiviral and immunomodulatory agent. There has been no microscopic (instead of phenomenological) model of its biological or pharmacological activity because of a lack of knowledge about its physicochemical nature. In the present work, the results of a study on the physicochemical properties of the HG macromolecules which makes it possible to qualitatively explain the biological activity and pharmacological properties of Panavir are presented. © 2012 Pleiades Publishing, Ltd.


Blagodatskikh I.V.,RAS Nesmeyanov Institute of Organoelement Compounds | Tikhonov V.E.,RAS Nesmeyanov Institute of Organoelement Compounds | Postnikov V.A.,Research Institute of Physicochemical Medicine | Kobitskaya (Ivanova) E.M.,RAS Nesmeyanov Institute of Organoelement Compounds | And 2 more authors.
Nanotechnologies in Russia | Year: 2014

Acrylamide-based hydrogel nanoparticles, which had hydrodynamic radii in aqueous dispersions of about 100-180 nm, were prepared by miniemulsion polymerization. The redox initiation at the interfacial boundary made it possible to conduct synthesis at an ambient temperature with a high rate and resulted in latexes of enhanced aggregative stability. The original method for nanoparticle functionalization by aminophenylboronic acid was elaborated for their application as affinity sorbents for 1,2-diols. The ability to reversibly bind ribonucleoside (inosine) and glycated protein (hemoglobin) has been demonstrated. The binding capacity relative to inosine and glycated hemoglobin was determined as a function of the phenylboronate group content. © 2014 Pleiades Publishing, Ltd.


Gritskova I.A.,Lomonosov Moscow University of Fine Chemical Technology | Adikanova D.B.,Kazakh National Technical University | Papkov V.S.,RAS Nesmeyanov Institute of Organoelement Compounds | Prokopov N.I.,Lomonosov Moscow University of Fine Chemical Technology | And 6 more authors.
Polymer Science - Series B | Year: 2016

The effect of synthesis conditions, such as the phase and concentration ratios of components, on the mechanism of styrene polymerization in the presence of the water-insoluble organosilicon surfactant α,ω-bis(10-carboxydecyl)polydimethylsiloxane and its mixture with the nonionic surfactant Pluronic F-68 is studied. © 2016, Pleiades Publishing, Ltd.


Piryazev A.P.,Research Institute of Physicochemical Medicine | Azizova O.A.,Research Institute of Physicochemical Medicine | Aseichev A.V.,Research Institute of Physicochemical Medicine | Dudnik L.B.,Research Institute of Physicochemical Medicine | Sergienko V.I.,Research Institute of Physicochemical Medicine
Bulletin of Experimental Biology and Medicine | Year: 2013

We studied the effect of gold nanoparticles on ROS production by leukocytes. ROS production was detected by luminol-dependent chemiluminescence (LDCL) of human peripheral blood leukocytes stimulated with opsonized zymosan. Nanoparticle size was 5, 10 and 30 nm. Simultaneous addition of nanoparticles and opsonized zymosan showed that 5-nm nanoparticles inhibited LDCL intensity in comparison with the control, when LDCL recording was conducted in the presence of opsonized zymosan. Increasing nanoparticle size from 5 up to 30 nm enhanced LDCL intensity. Preincubation of gold nanoparticles with autologous blood plasma increased LDCL intensity. In the control (without gold nanoparticles), blood plasma produced no activating effect on LDCL. We found that the effect of gold nanoparticles on leukocyte LDCL depended on nanoparticle size: 10- and 30-nm nanoparticles inhibited LDCL intensity in comparison with the control (incubation in the absence of nanoparticles) irrespective of the duration of incubation, while 5-nm gold nanoparticles had no effect on LDCL intensity. Incubation of gold nanoparticles with autologous plasma increased LDCL intensity if nanoparticle size was 30 and 10 nm. © 2013 Springer Science+Business Media New York.


Savinova T.A.,National Research Center | Il'Ina E.N.,Research Institute of Physicochemical Medicine | Sidorenko S.V.,Research Institute of Childhood Infections
Molecular Genetics, Microbiology and Virology | Year: 2010

Despite the growing level of resistance to Streptococcus pneumoniae infections, β-lactam antibiotics remain the drugs of choice treating these infections. The resistance of S. pneumoniae to these preparations is mediated by modifications of penicillin-binding proteins (PBPs), which are the targets of antibiotics action. The new approach to detecting mutations in the PBP 1A, 2B, and 2X genes based on the minise-quencing reaction followed by matrix-assisted laser desorption/ionization time of flight (MALDI-ToF) mass spectrometry has been developed in the present study. The evaluation of the prevalence of these mutations in clinical S. pneumoniae isolates (n = 194) with different levels of susceptibility to beta-lactam antibiotics has been performed. In summary, 24 different combinations of mutations (genotypes) have been detected in PBPs. All penicillin-susceptible isolates (n = 49, MIC ≤ 0.06 μg/ml) were characterized by the absence of mutations in all analyzed loci. In PBPs, mutations were detected in 133 (91.7%) out of 145 S. pneumoniae isolates with reduced susceptibility to penicillin (MIC > 0.06 μg/ml), which indicates the high diagnostic sensitivity of this approach. Isolates with MIC → 4 μg/ml (n = 20) possessed multiple mutations in all analyzed genes, which confirms the cumulative effects of the formation of penicillin resistance. At the same time, no association between the presence of mutations in PBP genes and decreased susceptibility to cefotaxime was shown, which makes it possible to suggest significant differences in molecular mechanisms of penicillins and cephalosporins resistance. The suggested method of S. pneumoniae genotyping is appropriate for the individual screening of the susceptibility of isolates to penicillin and the molecular monitoring of the resistance determinants in population. © 2010 Allerton Press, Inc.


PubMed | Research Institute of Physicochemical Medicine
Type: Journal Article | Journal: Bulletin of experimental biology and medicine | Year: 2013

We studied the effect of gold nanoparticles on ROS production by leukocytes. ROS production was detected by luminol-dependent chemiluminescence (LDCL) of human peripheral blood leukocytes stimulated with opsonized zymosan. Nanoparticle size was 5, 10 and 30 nm. Simultaneous addition of nanoparticles and opsonized zymosan showed that 5-nm nanoparticles inhibited LDCL intensity in comparison with the control, when LDCL recording was conducted in the presence of opsonized zymosan. Increasing nanoparticle size from 5 up to 30 nm enhanced LDCL intensity. Preincubation of gold nanoparticles with autologous blood plasma increased LDCL intensity. In the control (without gold nanoparticles), blood plasma produced no activating effect on LDCL. We found that the effect of gold nanoparticles on leukocyte LDCL depended on nanoparticle size: 10- and 30-nm nanoparticles inhibited LDCL intensity in comparison with the control (incubation in the absence of nanoparticles) irrespective of the duration of incubation, while 5-nm gold nanoparticles had no effect on LDCL intensity. Incubation of gold nanoparticles with autologous plasma increased LDCL intensity if nanoparticle size was 30 and 10 nm.


PubMed | Research Institute of Physicochemical Medicine
Type: Evaluation Studies | Journal: Journal of clinical microbiology | Year: 2010

The choice of adequate methods for epidemiological purposes remains a challenging problem in Neisseria gonorrhoeae molecular monitoring. In this study, the collection of geographically unrelated gonococci (n = 103) isolated in Russian clinics was comparably tested by (i) a traditional serotyping scheme, (ii) por typing, (iii) Neisseria gonorrhoeae multiantigen sequence typing (NG-MAST), and (iv) multilocus sequence typing (MLST). It is shown that, according to sequencing data, a third of the strains carried new porB1 alleles, as well as tbpB ones, and more than half of the samples had new sequence types (STs) as determined by NG-MAST or MLST. The discriminatory power for each typing method was calculated by using the Hunter-Gaston discriminatory index, D. Commonly, modern nucleic acid-based typing methods (por typing, NG-MAST, and MLST) appeared to be more efficient than the classical serotyping scheme. While the traditional serotyping gave a D value of 0.82, the por typing, NG-MAST, and MLST approaches yielded D values of 0.97, 0.98, and 0.91, respectively. Each typing technique revealed the distribution of gonococci slightly correlated with their geographical sources. However, only the MLST method STs were highly associated with certain phenotypes. Although ST1594, ST1892, and ST6720 were typical for susceptible gonococci, ST1901 and ST6716 were undoubtedly associated with a multidrug-resistant phenotype. We conclude that every tested nucleic acid-based typing method is suitable for N. gonorrhoeae molecular surveillance. However, the MLST method seems to serve large-scale epidemiological purposes, whereas the NG-MAST and por typing approaches are more appropriate for the investigation of local outbreaks.

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