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Zhang C.-M.,Research Institute of Orthopedics Surgery | Tian H.-L.,Research Institute of Orthopedics Surgery | Yun T.,Research Institute of Orthopedics Surgery | Yu S.-F.,Research Institute of Orthopedics Surgery | Sun X.-J.,Research Institute of Orthopedics Surgery
Chinese Journal of Tissue Engineering Research | Year: 2013

BACKGROUND: Preliminary studies have shown that heparin-chitosan-coated acellular bone matrix implantation can significantly improve the blood supply of bone defect region. Then, large-volume anticoagulant material implantation into vascular bundles can improve blood perfusion and vascularization processes or not? OBJECTIVE: To investigate the effects of heparin-chotisan-coated acellular bone matrix implantation combined with vascular bundles on hemoperfusion and vascularization in the early period of bone defects. METHODS: Twenty-five healthy New Zealand white rabbits were selected to prepare 20 mm long bone defect models of the bilateral radii. Heparin-chotisan-coated acellular bone matrix combined with vascular bundles (experimental group) was implanted into the right radius, and acellular bone matrix combined with vascular bundles (control group) was implanted into the left radius. CT perfusion imaging and histological observation were done at postoperative days 1, 3, 7, 14, 28. RESULTS AND CONCLUSION: Blood volume and blood flow were significantly higher in the experimental group than the control group from postoperative day 1 till day 28 (P < 0.05). Blood perfusion at the center of implant material and blood infiltration around the implant were found in the experimental and control groups, respectively. A large amount of red blood cells and nucleated cells within the composite material were visible in the experimental group, while permeated liquid was mainly seen in the control group at 1 day postoperatively. Within 3-7 days after implantation, new vessels appeared in the micropores of the experimental group and then increased gradually; in the control group, vascular thrombosis and luminal occlusion formed in the implanted vessels, wrapped with fibrotic surrounding tissues. The number of vessels within the implant was higher in the experimental group than the control group at each time point (P < 0.05). It indicates that implantation of heparin-chitosan-coated acellular bone matrix scaffold combined with vascular bundles can promote blood perfusion and early vascularization process.

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