Research Institute of Nutrition

Moscow, Russia

Research Institute of Nutrition

Moscow, Russia

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Kirbaeva N.V.,Research Institute of Nutrition | Sharanova N.E.,Research Institute of Nutrition | Vasil'ev A.V.,Research Institute of Nutrition | Pertsov S.S.,Moscow State University
Bulletin of Experimental Biology and Medicine | Year: 2017

We studied proteome profile of blood serum of Wistar rats with different behavioral activity immediately and in 1 and 3 days after acute stress on the model of 12-h immobilization during the nighttime. Comparative analysis of 2D-electrophoretograms revealed differences in the expression of serum proteins in non-stressed (control) and stressed (experimental) rats. We found 22 protein spots that characterized the proteomic features of blood serum in rats with different prognostic resistance to stress. Mass-spectrometry of isolated spots identified 6 functional proteins. Persistent proteome changes in the blood of animals at different stages after acute stress were determined. The specificity of proteomic characteristics of blood serum was shown in behaviorally passive and active rats during the post-stress period. These data extend the concept on specific protein markers for the formation of a negative emotional state and adaptive-and-compensatory processes in mammals with different sensitivity to stressogenic factors. © 2017 Springer Science+Business Media New York


Evstratova V.S.,Research Institute of Nutrition | Nikityuk D.B.,Research Institute of Nutrition | Riger N.A.,Research Institute of Nutrition | Fedyanina N.V.,Research Institute of Nutrition | Khanferyan R.A.,Research Institute of Nutrition
Bulletin of experimental biology and medicine | Year: 2016

In vitro production of immunoregulatory cytokines (IFN-α, IL-31, TNF-β, IL-17A, IL-7, IL-1RA, IL-1α, IL-10, IL-15, IL-21, IL-22, IL-23, IL-27, and IL-9) by dendritic cell cultures was compared in ski athletes and healthy donors. Effect of prolonged intense physical exercise on secretory activity of immune cells was investigated. In both groups, secretion of IL-1RA, IL-10, IL-1α by dendritic cells was revealed, but there were significant differences in IL-1RA, IL-1α content (p<0.05) with lower level in the group of athletes. Production of IL-17A and IL-7 by dendritic cells in the group of athletes was not detected. In athletes, several proinflammatory cytokines (IFN-α, IL-31, and TNF-β) were secreted by cells in high concentrations, in contrast to the control group. In both groups, dendritic cells did not secrete IL-15, IL-21, IL-22, IL-23, IL-27, and IL-9.


PubMed | RAS Gause Institute of New Antibiotics, Research Institute of Nutrition and RAS Shemyakin Ovchinnikov Institute of Bioorganic Chemistry
Type: Journal Article | Journal: Acta naturae | Year: 2016

Detection of aminoglycoside antibiotics by MS or HPLC is complicated, because a) carbohydrate molecules have low ionization ability in comparison with other organic molecules (particularly in MALDI-MS), and b) the lack of aromatics and/or amide bonds in the molecules makes common HPLC UV-detectors useless. Here, we report on the application of a previously developed method for amine derivatization with tris(2,6- dimethoxyphenyl)carbenium ion to selective modification of aminoglycoside antibiotics. Only amino groups bound to primary carbons get modified. The attached aromatic residue carries a permanent positive charge. This makes it easy to detect aminoglycoside antibiotics by MS-methods and HPLC, both as individual compounds and in mixtures.


PubMed | P K Anokhin Research Institute Of Normal Physiology and Research Institute of Nutrition
Type: Journal Article | Journal: Bulletin of experimental biology and medicine | Year: 2016

The dynamics of coenzyme Q10 concentration in the blood plasma, liver, and brain of passive and active rats was studied on the model of metabolic stress. This parameter was shown to differ in rats with various patterns of behavior. Dietary consumption of coenzyme Q10 in doses of 10 and 100 mg/kg body weight was followed by changes in its content in experimental animals.


PubMed | RAS A.N. Bach Institute of Biochemistry and Research Institute of Nutrition
Type: Journal Article | Journal: Bulletin of experimental biology and medicine | Year: 2016

The effects of water-dispersed gold nanoparticles (8.00.9 nm in diameter) on the rat small intestinal mucosa and Peyer plaques, liver, and spleen were studied by electron microscopy. Water-dispersed gold nanoparticles injected into isolated intestinal loop not only accumulated in the small intestinal mucosa and Peyer plaques, but also penetrated into other organs, e.g. liver and spleen. Ultrastructural changes in the cells (hyperplasia of endoplasmic reticulum) were detected in the studied organs.


Adzhiev D.D.,Moscow Center for Dennatovenerology and Cosmetology | Mal'tsev G.Yu.,Research Institute of Nutrition | Rumyantsev S.A.,Dmitrii Rogachev Center for Pediatric Hematology | Malyarenko E.N.,Moscow Center for Dennatovenerology and Cosmetology | Zatorskaya N.F.,Moscow Center for Dennatovenerology and Cosmetology
Sel'skokhozyaistvennaya Biologiya | Year: 2015

Antioxidant system (AOS) is the most important unit of the general biological defense system. In assessing its state the choice of adequate, stable and sensitive indicators is important to determine the products of lipid peroxidation (LPO) and antioxidant defense enzyme complex. Anti-oxidant Index (AOI) is based on detecting blood and erythrocyte lipid peroxidation products such as malondialdehyde, diene conjugates of polyunsaturated fatty acids, and assessing the activity of anti-oxidant enzymes (i.e. superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase) in erythrocyte membranes. The aim of this work was to evaluate the role of AOS in the mechanism of blood oxidative homeostasis in Soviet Chinchilla rabbits (males and females) during different periods of ontogeny. Studies were performed in bioclinic on 10 rabbits. Blood was sampled from animals at the age of 60, 120 and 180 days. In the blood plasma we determined the content of diene conjugates and malondialdehyde, antioxidants and low molecular weight α-tocopherol and retinol. In hemolysates the activity of antioxidant enzymes, i.e. glutathione reductase, glutathione peroxidase, catalase, superoxide dismutase, was evaluated. For the first time for the biological evaluation of anti-oxidant system of rabbits a combined integrated AOI was used, which allows to reveal not only changes in the AOS of blood, but also to determine the proportion of its individual factors in the on-togenesis of rabbits. A comparison of the content of cholesterol, glucose and triglycerides in blood plasma showed the differences between male and female rabbits in lipid and energy metabolism. At the age of 180 days an increase in cholesterol, triglycerides and glucose in males was 11.6 %, 27.2 % (P ≤ 0.001) and 12.9 % (P ≤ 0.01), respectively, and in the females it was 14.7 %, 27.4 % (P ≤ 0.001) and 13.0 % (P ≤ 0.05), respectively. At the age of 120 and 180 days in the animals an insignificant reduction in all indicators was observed compared with the values obtained in the 60 day old rabbits. α-Tocopherol content in males aged 120 and 180 days increased by 14.4 and 21.9 %, respectively, and the retinol level was 8.3 and 29.2 % higher compared to the initial (day 60) values. In females there was a significant increase in the concentration of α-tocopherol by 14.9 and 18.9 %, respectively, and in retinol by 13.6 and 50.0 %, respectively, at days 120 and 180. Revealed changes in the overall and partial AOI in ontogeny indicate the initial activation of compensatory mechanism of antixidation in early ontogenesis and fürther decrease in a compensatory capacity of the antioxidant defense system in blood. Thus, the general index of the AOI in 4 month old rabbit males and females increased by 12-20 % and 4-12 %, respectively. A positive index value indicates the relatively high antioxidant protection despite the trend to reducing activity of the enzyme protection. In the 6 month old animals the integrated combined AOI values were negative, being typical for the state of oxidative stress. In males these changes were more evident and reliable. Usage of AOI index may be informative in assessment of the state of protective systems both in farm and laboratory animals.


Sharafetdinov K.K.,Research Institute of Nutrition | Lapik I.A.,Research Institute of Nutrition | Vorozhko I.V.,Research Institute of Nutrition | Tutelyan V.A.,Research Institute of Nutrition
Bulletin of Experimental Biology and Medicine | Year: 2015

The paper presents the basic criteria for glycemic control in type 2 diabetes mellitus, the data on the relationship between postprandial glycemia and the development of the late vascular complications, and methods for evaluating the glycemic index of foodstuffs and dishes in order to optimize the diets and improve the efficiency of therapeutic measures in this disease. © 2015, Springer Science+Business Media New York.


Borodina S.V.,Research Institute of Nutrition | Gapparova K.M.,Research Institute of Nutrition | Zainudinov Z.M.,Research Institute of Nutrition | Grigorian O.N.,Research Institute of Nutrition
Obesity and Metabolism | Year: 2016

The most common reasons that cause obesity are eating disorders (overeating), genetic predisposition, sedentary lifestyle (lack of exercise), disorders of the endocrine system, and environmental factors. There is evidence of an obvious relationship of high consumption of sugary drinks and weight gain. Since 1990, there has been considerable growth in the number of obese people in the first place associated with the promotion of soft drinks. According to a study in Finnish diabetes prevention average physical activity and change of diet (1200-1800 kcal) of total fat intake with less than 30% saturated fat, including less than 10%, leading to long-term loss of excess weight (within 4 years). Many studies have demonstrated the impossibility of a single template approach to the determination of optimal diets for patients with overweight and obesity which has been shown in various studies on gene polymorphisms are associated with obesity, and their interaction. This article provides an overview of current data on the genetics of obesity covering the main provisions of the study of candidate genes, such as PPARG, FABP2, ADRB 2, ADRB3. The role nutrigenetics in the creation of individual programs of weight control and weight loss. But the question of the direct role of genetic factors in the development of obesity remains controversial, since one can not ignore the impact of environmental factors, such as lifestyle, diet, physical activity, stress, and harmful habits. To understand the mechanism of the relationship between genetic factors, environmental factors, and obesity, one needs to carry out research not only on the population level, but also in certain groups of people (ethnic, racial, age).


PubMed | Research Institute of Nutrition
Type: Journal Article | Journal: Bulletin of experimental biology and medicine | Year: 2016

In vitro production of immunoregulatory cytokines (IFN-, IL-31, TNF-, IL-17A, IL-7, IL-1RA, IL-1, IL-10, IL-15, IL-21, IL-22, IL-23, IL-27, and IL-9) by dendritic cell cultures was compared in ski athletes and healthy donors. Effect of prolonged intense physical exercise on secretory activity of immune cells was investigated. In both groups, secretion of IL-1RA, IL-10, IL-1 by dendritic cells was revealed, but there were significant differences in IL-1RA, IL-1 content (p<0.05) with lower level in the group of athletes. Production of IL-17A and IL-7 by dendritic cells in the group of athletes was not detected. In athletes, several proinflammatory cytokines (IFN-, IL-31, and TNF-) were secreted by cells in high concentrations, in contrast to the control group. In both groups, dendritic cells did not secrete IL-15, IL-21, IL-22, IL-23, IL-27, and IL-9.


PubMed | Research Institute of Nutrition
Type: Journal Article | Journal: Bulletin of experimental biology and medicine | Year: 2015

The paper presents the basic criteria for glycemic control in type 2 diabetes mellitus, the data on the relationship between postprandial glycemia and the development of the late vascular complications, and methods for evaluating the glycemic index of foodstuffs and dishes in order to optimize the diets and improve the efficiency of therapeutic measures in this disease.

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