Research Institute of Medical science

Gwangju, South Korea

Research Institute of Medical science

Gwangju, South Korea
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Ha S.-A.,Research Institute of Medical Science | Kim H.J.,Research Institute of Medical Science | Shin S.M.,Research Institute of Medical Science | Kim H.K.,Research Institute of Medical Science | And 6 more authors.
Experimental and Molecular Pathology | Year: 2010

The proliferative capacity of tumor cells is a characteristic feature in the whole growing tumors. Many pathologists and clinicians have used the estimation of cell proliferation for prognostic information. Minichromosome maintenance protein 3 (MCM3) is known to have a role on the initiation and regulation of DNA replication during cell cycle. The aim of this study was to evaluate the potential applicability of one of the MCM proteins, MCM3, as a proliferation marker in papillary thyroid carcinoma (PTC) with correlation to clinicopathological parameters. We performed the immunohistochemical analysis for MCM3 and Ki-67 in 60 cases of PTC and Western blot analysis for MCM3 expression in 6 PTCs and normal thyroid tissues. The comparison of MCM3 labeling index (LI) to tumor size (P = 0.031) and extrathyroidal extension (P = 0.037) was statistically significant while that of Ki-67 LI to them was not. Moreover, a significant association was not observed between MCM3 and Ki-67, but the MCM3 LI was considerably higher. Western blot analyses revealed that the MCM3 protein expression levels were overexpressed in all PTCs. On the contrary, the levels of MCM3 were very low or absent in all normal thyroid tissues. Our results indicate that MCM3 may be a more reliable proliferation marker than Ki-67 in accessing the growth of tumor and evaluating tumor aggressiveness of PTC. © 2009 Elsevier Inc. All rights reserved.


Kim J.Y.,Sogang University | Kim J.-H.,Sogang University | Park B.-L.,SNP Genetics Inc. | Pasaje C.F.A.,Sogang University | And 12 more authors.
Journal of Asthma | Year: 2012

Background. The discoidin domain receptor tyrosine kinase 1 (DDR1) is positioned within the major histocompatibility complex (MHC) region which plays an important role in the immune system. In addition, DDR1 has been elucidated to be downregulated during the epithelialmesenchymal transition of bronchial epithelium. Objective. To investigate the potential genetic associations between DDR1 and aspirin-exacerbated respiratory disease (AERD), this study conducted association studies of DDR1 single nucleotide polymorphisms (SNPs) with AERD and the obstructive symptom of forced expiratory volume in 1 s (FEV1) decline after aspirin provocation. Methods. Nine common SNPs were genotyped in 93 AERD patients and 96 aspirin-tolerant asthma (ATA) controls. The genotype distributions of all loci were in HardyWeinberg equilibrium (HWE; p >.05). Results. In the results of logistic analyses using age, sex, smoking status, and atopy as covariates, DDR1 rs1264320 in the intronic region showed a potent association signal with FEV1 decline by aspirin provocation in asthmatics of this study even after corrections for multiple testing (p =.003 and corrected p =.01). However, the variants of DDR1 were not significantly associated with the AERD development (corrected p >.05). On further comparison of FEV1 decline by aspirin provocation between AERD and ATA, the variant rs1264320 was found to be associated with the FEV1 decline of ATA rather than AERD. Conclusion. Despite the need for further functional evaluations and replications, we conclude that DDR1 polymorphisms are not likely to contribute to predispositions of AERD, but may be potentially associated with FEV1 decline by aspirin provocation in asthmatics. © 2012 Informa Healthcare USA, Inc.


Han S.-J.,Research Institute of Medical science | Han S.-J.,Chonnam National University | Ahn Y.,University of Calgary | Park I.,Research Institute of Medical science | And 9 more authors.
Methods | Year: 2015

PTEN is reversibly oxidized in various cells by exogenous hydrogen peroxide as well as by endogenous hydrogen peroxide generated when cells are stimulated with growth factors, cytokines and hormones. A gel mobility shift assay showed that oxidized PTEN migrated more rapidly than reduced PTEN on a non-reducing SDS-PAGE gel. Oxidized PTEN was reduced when treated with dithiothreitol. Supplementation of N-ethylmaleimide in the cell lysis buffer was critical for the apparent bands of oxidized and reduced PTEN. Formation of oxidized PTEN was abolished when the active site Cys124 or nearby Cys71 was replaced with Ser suggesting that Cys124 and Cys71 are involved in the formation of an intramolecular disulfide bond. These results show that the mobility shift assay is a convenient method to analyze the redox state of PTEN in cells. © 2015 Elsevier Inc.


Lee D.H.,Chonnam National University | Yoon T.M.,Chonnam National University | Lee J.K.,Chonnam National University | Joo Y.E.,Research Institute of Medical science | Lim S.C.,Chonnam National University
Journal of Craniofacial Surgery | Year: 2013

The most common presentation of herpes zoster in the head and neck region is called Ramsay Hunt syndrome (RHS), which rarely accompanies multiple cranial neuropathy. Herpes zoster also involves the mucous membrane of the tongue, palate, pharynx, and larynx. Herpes zoster infection of the larynx accompanied by Ramsay Hunt syndrome with cranial polyneuropathy is extremely rare, with only few reported cases in the literature. At the time of this report, a review of the medical literature disclosed 4 reported cases of herpes zoster laryngitis accompanied by Ramsay Hunt syndrome. Herein, we present 2 additional cases and report the clinical outcome of cranial polyneuropathy with a review of the literature. Copyright © 2013 by Mutaz B. Habal, MD.


Kim G.-E.,Chonnam National University | Kim G.-E.,Research Institute of Medical science | Lee K.H.,Chonnam National University | Lee K.H.,Research Institute of Medical science | And 14 more authors.
Virchows Archiv | Year: 2011

Promoter hypermethylation has been shown to be a common mechanism for inactivation of tumor suppressor genes in breast cancer. The aim of this study was to investigate the prevalence of Slit2 promoter hypermethylation in both the tumor and serum samples of breast cancer patients with ductal carcinoma in situ (DCIS) or invasive breast carcinoma (IBC). The methylation status of Slit2 was investigated in 210 tissue samples (15 breast with no pathological findings, 26 DCIS, and 169 IBC samples) and 123 corresponding serum samples (15 breast with no pathological findings, 26 DCIS, and 82 IBC samples) using methylation-specific polymerase chain reaction. Immunohistochemical staining for Slit2 was also performed using tissue microarray blocks to determine whether Slit2 promoter hypermethylation correlated with loss of Slit2 expression. Slit2 promoter hypermethylation was not detected in breast tissue and serum samples from patients with no pathological findings. DCIS or IBC showed a statistically higher frequency of Slit2 promoter hypermethylation compared to breast with no pathological findings in both the tissue and serum samples; however, there were no statistically significant differences between DCIS and IBC samples. Similar Slit2 promoter hypermethylation patterns were seen in the tissue samples and corresponding serum specimens (p < 0.001). Slit2 promoter hypermethylation was associated with loss of Slit2 expression. These results suggest that Slit2 promoter hypermethylation appears to be responsible for functionally silencing Slit2 expression. Slit2 promoter hypermethylation may be considered as a possible serum marker for early detection of breast cancer. © 2011 Springer-Verlag.


Kim J.M.,Research Institute of Medical Science | Kim J.M.,Konkuk University | Park S.W.,Research Institute of Medical Science | Park S.W.,Konkuk University | And 11 more authors.
Journal of Pharmacological Sciences | Year: 2015

MK801 (dizocilpine), a phencyclidine (PCP) derivative, is a potent noncompetitive antagonist of the N-Methyl-D-aspartate receptor (NMDAr). Another PCP derivative, ketamine, was reported to block voltage-gated K+ (Kv) channels, which was independent of NMDAr function. Kv currents are major regulators of the membrane potential (Em) and excitability of muscles and neurons. Here, we investigated the effect of MK801 on the Kv channels and Em in rat mesenteric arterial smooth muscle cells (RMASMCs). We used the whole-cell patch clamp technique to analyze the effect of MK801 enantiomers on Kv channels and Em. (+)MK801 inhibited Kv channels in a concentration-dependent manner (IC50 of 89.1 ± 13.1 μM, Hill coefficient of 1.05 ± 0.08). The inhibition was voltage- and state- independent. (+)MK801 didn't influence steady-state activation and inactivation of Kv channels. (+)MK801 treatment depolarized Em in a concentration-dependent manner and concomitantly decreased membrane conductance. (-)MK801 also similarly inhibited the Kv channels (IC50 of 134.0 ± 17.5 μM, Hill coefficient of 0.87 ± 0.09). These results indicate that MK801 directly inhibits the Kv channel in a state-independent manner in RMASMCs. This MK801-mediated inhibition of Kv channels should be considered when assessing the various pharmacological effects produced by MK801, such as schizophrenia, neuroprotection, and hypertension. © 2014 Japanese Pharmacological Society.


Joo J.D.,Catholic University of Korea | Choi J.W.,Catholic University of Korea | In J.H.,Catholic University of Korea | Jung H.S.,Catholic University of Korea | And 6 more authors.
European Journal of Anaesthesiology | Year: 2011

Background and objective Rats which have undergone spinal nerve ligation (SNL) display increases in the expression of extracellular signal-regulated kinase (ERK 1/2) and cyclic AMP response element-binding (CREB) protein. The present study was designed to determine whether lidocaine has a beneficial effect on the treatment of neuropathic pain by analysing related proteins. Methods Twenty-four male Sprague-Dawley rats were randomly allocated to three groups (eight per group): shamoperated (control) group, a neuropathic pain and normal saline group (NP+NS), a neuropathic pain and lidocaine group (NP+Lido, 2mgkg -1h -1). Anaesthetised rats received left L5 and L6 SNL. The mechanical withdrawal threshold test was performed 7 days after SNL and for 7 days with the pump implanted (saline or lidocaine). At post-implanted pump day 7, their brains and spinal cords were harvested. ERK 1/2, CREB proteins and mRNA amounts of pro-inflammatory cytokines (tumour necrosis factor a, intercellular adhesion molecule 1, monocyte chemo-attractive protein 1 and macrophage inflammatory protein 2) were assessed by immunoblotting or reverse transcriptase-PCR on samples collected from the three groups. Results Lidocaine increased the mechanical withdrawal threshold of a neuropathic rats. In only spinal tissues, ERK 1/2 and CREB proteins in the NP+Lido group was significantly reduced to 39%, and 48% in comparison with the NPRNS group. The NP+Lido group showed a significant reduction in mRNA amounts of pro-inflammatory cytokines compared with the NP+NS group (P<0.05). Conclusion These results suggest that lidocaine therapy may be effective in treating neuropathic pain after spinal nerve injury, and that these effects may occur via suppression of ERK 1/2 and CREB signalling proteins and anti-inflammatory effects. Eur J Anaesthesiol 2011;28:106-111 Published online 11 November 2010 ©2011 Copyright European Society of Anaesthesiology.


Koh Y.-I.,Research Institute of Medical science | Shim J.-U.,Research Institute of Medical science | Wi J.-O.,Research Institute of Medical science | Han E.-R.,Research Institute of Medical science | And 4 more authors.
Human Immunology | Year: 2010

Invariant natural killer T (iNKT) cells have been reported to play a role in the pathogenesis of murine asthma. However, the role for iNKT cells in the pathogenesis of human asthma is not defined. In this study we aimed to determined how blood iNKT cells are associated with atopy in asthmatic individuals. Peripheral blood mononuclear cells were isolated from 45 asthmatic subjects. iNKT cells were stained with 6B11 mAb, anti-TCRvα24 mAb, or α-galactosylceramide (GalCer)-loaded CD1d- tetramer and analyzed with flow-cytometric assays. Increased serum total IgE or one or more positive skin reactions to common allergens were used as atopic indexes. Asthmatic subjects with IgE > 500 IU/ml showed lower frequency of CD4+ 6B11+ iNKT cells (p < 0.01) or CD4+ Vα24+ iNKT cells (p < 0.01) compared with subjects with IgE ≤ 500 IU/ml. Asthmatic subjects with atopy on skin tests had lower frequency of CD4+ α-GalCer-loaded CD1d- tetramer+ iNKT cells compared with those without atopy (p < 0.05). The frequency of CD4+ Vα24+ iNKT cells was negatively correlated with total IgE in asthmatic subjects (r = -0.33, p < 0.05). In summary, blood CD4+ iNKT cells were inversely associated with atopic indexes in asthmatic individuals. We hypothesize that blood CD4+ iNKT cells might behave like Th1-like iNKT cells in human asthma. © 2010 American Society for Histocompatibility and Immunogenetics.


Sakata Y.,Kanazawa Medical University | Kitayama A.,Kanazawa Medical University | Yoshimura R.,Kanazawa Medical University | Anzawa K.,Kanazawa Medical University | And 6 more authors.
Journal of Dermatology | Year: 2015

We describe a case of cutaneous phaeohyphomycosis in a 61-year-old man receiving re-dialysis treatment for renal failure of a transplanted kidney. He was immunocompromised with steroid and cyclosporin A at onset of an asymptomatic abscess on his right forearm. The abscess arose at the site of a skin injury approximately 1 year prior. Grayish molds isolated from the lesion were morphologically compatible with Phaeoacremonium sp. but nucleotide sequence data of internal transcribed spacer regions of ribosomal RNA gene, actin and β-tubulin genes were unlike those of any described species. He was successfully treated with a total of 3 weeks of liposomal amphotericin B, but died of pneumonia approximately 3 months after cure of phaeohyphomycosis. © 2014 Japanese Dermatological Association.


PubMed | Research Institute of Medical Science
Type: Case Reports | Journal: The Journal of dermatology | Year: 2015

We describe a case of cutaneous phaeohyphomycosis in a 61-year-old man receiving re-dialysis treatment for renal failure of a transplanted kidney. He was immunocompromised with steroid and cyclosporin A at onset of an asymptomatic abscess on his right forearm. The abscess arose at the site of a skin injury approximately 1 year prior. Grayish molds isolated from the lesion were morphologically compatible with Phaeoacremonium sp. but nucleotide sequence data of internal transcribed spacer regions of ribosomal RNA gene, actin and -tubulin genes were unlike those of any described species. He was successfully treated with a total of 3 weeks of liposomal amphotericin B, but died of pneumonia approximately 3 months after cure of phaeohyphomycosis.

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