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Joo J.D.,Catholic University of Korea | Choi J.W.,Catholic University of Korea | In J.H.,Catholic University of Korea | Jung H.S.,Catholic University of Korea | And 6 more authors.
European Journal of Anaesthesiology | Year: 2011

Background and objective Rats which have undergone spinal nerve ligation (SNL) display increases in the expression of extracellular signal-regulated kinase (ERK 1/2) and cyclic AMP response element-binding (CREB) protein. The present study was designed to determine whether lidocaine has a beneficial effect on the treatment of neuropathic pain by analysing related proteins. Methods Twenty-four male Sprague-Dawley rats were randomly allocated to three groups (eight per group): shamoperated (control) group, a neuropathic pain and normal saline group (NP+NS), a neuropathic pain and lidocaine group (NP+Lido, 2mgkg -1h -1). Anaesthetised rats received left L5 and L6 SNL. The mechanical withdrawal threshold test was performed 7 days after SNL and for 7 days with the pump implanted (saline or lidocaine). At post-implanted pump day 7, their brains and spinal cords were harvested. ERK 1/2, CREB proteins and mRNA amounts of pro-inflammatory cytokines (tumour necrosis factor a, intercellular adhesion molecule 1, monocyte chemo-attractive protein 1 and macrophage inflammatory protein 2) were assessed by immunoblotting or reverse transcriptase-PCR on samples collected from the three groups. Results Lidocaine increased the mechanical withdrawal threshold of a neuropathic rats. In only spinal tissues, ERK 1/2 and CREB proteins in the NP+Lido group was significantly reduced to 39%, and 48% in comparison with the NPRNS group. The NP+Lido group showed a significant reduction in mRNA amounts of pro-inflammatory cytokines compared with the NP+NS group (P<0.05). Conclusion These results suggest that lidocaine therapy may be effective in treating neuropathic pain after spinal nerve injury, and that these effects may occur via suppression of ERK 1/2 and CREB signalling proteins and anti-inflammatory effects. Eur J Anaesthesiol 2011;28:106-111 Published online 11 November 2010 ©2011 Copyright European Society of Anaesthesiology. Source

Lee D.H.,Chonnam National University | Yoon T.M.,Chonnam National University | Lee J.K.,Chonnam National University | Joo Y.E.,Research Institute of Medical science | Lim S.C.,Chonnam National University
Journal of Craniofacial Surgery | Year: 2013

The most common presentation of herpes zoster in the head and neck region is called Ramsay Hunt syndrome (RHS), which rarely accompanies multiple cranial neuropathy. Herpes zoster also involves the mucous membrane of the tongue, palate, pharynx, and larynx. Herpes zoster infection of the larynx accompanied by Ramsay Hunt syndrome with cranial polyneuropathy is extremely rare, with only few reported cases in the literature. At the time of this report, a review of the medical literature disclosed 4 reported cases of herpes zoster laryngitis accompanied by Ramsay Hunt syndrome. Herein, we present 2 additional cases and report the clinical outcome of cranial polyneuropathy with a review of the literature. Copyright © 2013 by Mutaz B. Habal, MD. Source

Kim J.Y.,Sogang University | Kim J.-H.,Sogang University | Park B.-L.,SNP Genetics Inc. | Pasaje C.F.A.,Sogang University | And 12 more authors.
Journal of Asthma | Year: 2012

Background. The discoidin domain receptor tyrosine kinase 1 (DDR1) is positioned within the major histocompatibility complex (MHC) region which plays an important role in the immune system. In addition, DDR1 has been elucidated to be downregulated during the epithelialmesenchymal transition of bronchial epithelium. Objective. To investigate the potential genetic associations between DDR1 and aspirin-exacerbated respiratory disease (AERD), this study conducted association studies of DDR1 single nucleotide polymorphisms (SNPs) with AERD and the obstructive symptom of forced expiratory volume in 1 s (FEV1) decline after aspirin provocation. Methods. Nine common SNPs were genotyped in 93 AERD patients and 96 aspirin-tolerant asthma (ATA) controls. The genotype distributions of all loci were in HardyWeinberg equilibrium (HWE; p >.05). Results. In the results of logistic analyses using age, sex, smoking status, and atopy as covariates, DDR1 rs1264320 in the intronic region showed a potent association signal with FEV1 decline by aspirin provocation in asthmatics of this study even after corrections for multiple testing (p =.003 and corrected p =.01). However, the variants of DDR1 were not significantly associated with the AERD development (corrected p >.05). On further comparison of FEV1 decline by aspirin provocation between AERD and ATA, the variant rs1264320 was found to be associated with the FEV1 decline of ATA rather than AERD. Conclusion. Despite the need for further functional evaluations and replications, we conclude that DDR1 polymorphisms are not likely to contribute to predispositions of AERD, but may be potentially associated with FEV1 decline by aspirin provocation in asthmatics. © 2012 Informa Healthcare USA, Inc. Source

Sakata Y.,Kanazawa Medical University | Kitayama A.,Kanazawa Medical University | Yoshimura R.,Kanazawa Medical University | Anzawa K.,Kanazawa Medical University | And 6 more authors.
Journal of Dermatology | Year: 2015

We describe a case of cutaneous phaeohyphomycosis in a 61-year-old man receiving re-dialysis treatment for renal failure of a transplanted kidney. He was immunocompromised with steroid and cyclosporin A at onset of an asymptomatic abscess on his right forearm. The abscess arose at the site of a skin injury approximately 1 year prior. Grayish molds isolated from the lesion were morphologically compatible with Phaeoacremonium sp. but nucleotide sequence data of internal transcribed spacer regions of ribosomal RNA gene, actin and β-tubulin genes were unlike those of any described species. He was successfully treated with a total of 3 weeks of liposomal amphotericin B, but died of pneumonia approximately 3 months after cure of phaeohyphomycosis. © 2014 Japanese Dermatological Association. Source

Han S.-J.,Research Institute of Medical science | Han S.-J.,Chonnam National University | Ahn Y.,University of Calgary | Park I.,Research Institute of Medical science | And 9 more authors.
Methods | Year: 2015

PTEN is reversibly oxidized in various cells by exogenous hydrogen peroxide as well as by endogenous hydrogen peroxide generated when cells are stimulated with growth factors, cytokines and hormones. A gel mobility shift assay showed that oxidized PTEN migrated more rapidly than reduced PTEN on a non-reducing SDS-PAGE gel. Oxidized PTEN was reduced when treated with dithiothreitol. Supplementation of N-ethylmaleimide in the cell lysis buffer was critical for the apparent bands of oxidized and reduced PTEN. Formation of oxidized PTEN was abolished when the active site Cys124 or nearby Cys71 was replaced with Ser suggesting that Cys124 and Cys71 are involved in the formation of an intramolecular disulfide bond. These results show that the mobility shift assay is a convenient method to analyze the redox state of PTEN in cells. © 2015 Elsevier Inc. Source

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