Time filter

Source Type

Korzaia L.I.,Research Institute of Medical Primatology | Keburia V.V.,Research Institute of Medical Primatology | Dogadov D.I.,Research Institute of Medical Primatology | Lapin B.A.,Research Institute of Medical Primatology | And 2 more authors.
Voprosy Virusologii | Year: 2016

Serum from humans (n = 646) and monkeys (n = 1867) collected during the period 1999-2013 was tested by enzyme immunoassay. Anti-HEV IgG was detected significantly more frequently (P > 0.001) in rhesus macaques (Macaca mulatta) - 45.1 ± 1.6% (n = 1001) than in cynomolgus macaques (M. fascicularis) 16.2 ± 1.8% (n = 426). Single seropositive individuals were found among M. nemestrina - 4.0±2.8% (n = 50). Anti-HEV was not detected in the sera of green monkeys (Chlorocebus aethiops) -n = 162, Papio hamadryas (n = 124), and Papio anubis - n = 104. The presence of the anti-HEV IgM indicating the cases of fresh infection in Macaca mulatta - 2.1 ± 0.5% (n = 717) and M. fascicularis - 3.5 ± 1.3% (n = 266) is of great significance. The overall frequency of detection of the anti-HEV IgG among the staff of the Adler Primate Center - 6.8 ± 2.3% (n = 118) was significantly lower (P £ 0.001) than among the population of the Greater Sochi - 15.9% ± 1.6 (n = 528). It is important that only in patients of medical institutions (clinic, hospital, cancer center), anti-HEV IgM were detected (2.7-11.8%) along with anti-HEV IgG (15-23.5%), thereby indicating the presence of acute cases of HEV infection among this population. HEV RNA was not detected in the serum of anti-HEV IgM-positive people and monkeys. Seroepidemiological data do not confirm the assumption on the ability of seropositive monkeys of Macaca genus to be a natural reservoir of HEV infection for humans.


Kuevda E.V.,Kuban State Medical University | Gubareva E.A.,Kuban State Medical University | Krasheninnikov S.V.,RAS Research Center Kurchatov Institute | Grigoriev T.E.,RAS Research Center Kurchatov Institute | And 9 more authors.
Doklady Biochemistry and Biophysics | Year: 2016

The effect of decellularization on the biomechanical properties of macaque lungs was studied. The quality of the biological scaffold was additionally assessed by morphological methods, and the contents of extracellular matrix (ECM) fibers were determined both qualitatively and quantitatively. Histological analysis revealed no damage of structural integrity of ECM components, but the scaffold elasticity significantly decreased, which was confirmed by the changes in the hysteresis loop without a concomitant decrease in peak loads, with the mechanical strength of the samples being retained. These changes require taking additional measures to prevent a decrease in the effective lung volume. © 2016, Pleiades Publishing, Ltd.


Sotnichenko A.S.,Laboratory of fundamental research | Gubareva E.A.,Laboratory of fundamental research | Kuevda E.V.,Laboratory of fundamental research | Gumenyuk I.S.,Laboratory of fundamental research | And 2 more authors.
Medical News of North Caucasus | Year: 2016

Decellularization is used in biomedical engineering to isolate the extracellular matrix (ECM) from its inhabiting cells, preserving the ECM of the scaffold, which could be used for biological non-immunogenic and non- Toxic scaffolds obtainment for creation of full-fledged organs and tissues. Taking into account the importance of transferring results into clinic, the model of the non-human primates is the most suitable model for carrying out preclinical research. We have developed two protocols of Macaca mulatta esophagus decellularization by the modified detergent and enzymatic method differing in samples storage conditions, preparation for decellularization process, and also composition and duration of decellularizing agents' exposure. The obtained scaffolds maintain anatomical and morphological properties of a native esophagus as well as components of an ECM. The DNA-quantity substantially decreases when using detergents and enzymes. At the same time it is shown that organs' freezing at 30 °C requires protocol modification for lengthening of transportation and storage time and the subsequent thawing for decellularization. © Group of authors, 2016.


PubMed | Research Institute of Medical Primatology, Kuban State Medical University and RAS Research Center Kurchatov Institute
Type: Journal Article | Journal: Doklady. Biochemistry and biophysics | Year: 2016

The effect of decellularization on the biomechanical properties of macaque lungs was studied. The quality of the biological scaffold was additionally assessed by morphological methods, and the contents of extracellular matrix (ECM) fibers were determined both qualitatively and quantitatively. Histological analysis revealed no damage of structural integrity of ECM components, but the scaffold elasticity significantly decreased, which was confirmed by the changes in the hysteresis loop without a concomitant decrease in peak loads, with the mechanical strength of the samples being retained. These changes require taking additional measures to prevent a decrease in the effective lung volume.


Karaulov A.,Moscow Medical Academy | Aleshkin V.,Garbichevsky Moscow Gn Research Institute Of Epidemiology And Micro Biology | Slobodenyuk V.,Garbichevsky Moscow Gn Research Institute Of Epidemiology And Micro Biology | Grechishnikova O.,Garbichevsky Moscow Gn Research Institute Of Epidemiology And Micro Biology | And 11 more authors.
Infectious Diseases in Obstetrics and Gynecology | Year: 2010

Based on the results of the comparative analysis concerning relatedness and evolutional difference of the 16S-23S nucleotide sequences of the middle ribosomal cluster and 23S rRNA I domain, and based on identification of phylogenetic position for Chlamydophila pneumoniae and Chlamydia trichomatis strains released from monkeys, relatedness of the above stated isolates with similar strains released from humans and with strains having nucleotide sequences presented in the GenBank electronic database has been detected for the first time ever. Position of these isolates in the Chlamydiaceae family phylogenetic tree has been identified. The evolutional position of the investigated original Chlamydia and Chlamydophila strains close to analogous strains from the Gen-Bank electronic database has been demonstrated. Differences in the 16S-23S nucleotide sequence of the middle ribosomal cluster and 23S rRNA I domain of plasmid and nonplasmid Chlamydia trachomatis strains released from humans and monkeys relative to different genotype groups (group B-B, Ba, D, Da, E, L1, L2, L2a; intermediate group-F, G, Ga) have been revealed for the first time ever. Abnormality in incA chromosomal gene expression resulting in Chlamydia life development cycle disorder, and decrease of Chlamydia virulence can be related to probable changes in the nucleotide sequence of the gene under consideration. Copyright © 2010 Alexander Karaulov et al.


Karaulov A.V.,First Medical University | Slobodenyuk V.V.,Gamaleya Institute of Epidemiology and Microbiology | Aleshkin V.A.,Gamaleya Institute of Epidemiology and Microbiology | Grechishnikova O.G.,Gamaleya Institute of Epidemiology and Microbiology | And 10 more authors.
Vestnik Rossiiskoi Akademii Meditsinskikh Nauk | Year: 2011

Based on the results of the comparative analysis concerning relatedness and evolutional difference of the 16S - 23S nucleotide sequences of the middle ribosomal cluster and 23S rRNA I domain, and based on identification of phylogenetic position for Chlamydophila pneumoniae and Chlamydia trichomatis strains released from monkeys, relatedness of the above stated isolates with similar strains released from humans and with strains having nucleotide sequences presented in the GenBank electronic database has been detected for the first time ever. Position of these isolates in the Chlamydiaceae family phylogenetic tree has been identified. The evolutional position of the investigated original Chlamydia and Chlamydophila strains close to analogous strains from the GenBank electronic database has been demonstrated. Differences in the 16S - 23S nucleotide sequence of the middle ribosomal cluster and 23S rRNA I domain of plasmid and non-plasmid Chlamydia trachomatis strains released from humans and monkeys relative to different genotype groups (group B-B, Ba, D, Da, E, L1, L2, L2a; intermediate group - F, G, Ga) have been revealed for the first time ever. Abnormality in incA chromosomal gene expression resulting in Chlamydia life and development cycle disorder and decrease of Chlamydia virulence can be related to probable changes in the nucleotide sequence of the gene under consideration.


Agrba V.Z.,Research Institute of Medical Primatology | Porkhanov V.A.,Research Institute of Medical Primatology | Karal-ogly D.D.,Research Institute of Medical Primatology | Leontyuk A.V.,Research Institute of Medical Primatology | And 8 more authors.
Bulletin of Experimental Biology and Medicine | Year: 2016

Culture of mesenchymal stem cells isolated from the bone marrow of primates by their characteristics met the requirements of stem cells. It was shown that transplantation of allogeneic mesenchymal stem cells (2 million cells per 1 kg body weight) immediately after ligation of the left anterior descending coronary artery between the middle and upper thirds led to neovascularization and capillarization of the ischemic myocardium. © 2016 Springer Science+Business Media New York


Lapin B.A.,Research Institute of Medical Primatology | Yakovleva L.A.,Research Institute of Medical Primatology | Dzhikidze E.K.,Research Institute of Medical Primatology | Gvozdik T.E.,Research Institute of Medical Primatology | And 3 more authors.
Journal of Medical Primatology | Year: 2015

Background: In spring 2009 in Adler colony of the Institute of Medical Primatology, a large enzootic outbreak of acute intestine infection associated with pathogenic E. coli occurred and caused 5% mortality of population (209 animals). Methods: The epidemiological analysis, bacteriological investigation, postmortem examination, histological analysis, and PCR were used to identify the infectious agent. Results: Marked hemorrhagic diathesis, lethargy, dehydration, diarrhea with blood, wasting, and sometimes dystrophic changes in articular cartilages were noted. Morphologically, hemorrhagic enterocolitis and massive hemorrhages were found. PCR investigation of bacteriologically isolated E. coli characterized it as enteropathogenic and enteroinvasive E. coli. Conclusions: The outbreak in Adler colony slightly differed from similar outbreak in Florida in 2014 by more marked hemorrhagic diathesis and articular changes in some monkeys caused by polyavitaminosis developed in the course of infection. Sensitive to infection were M. mulatta, M. fascicularis, Cercopithecus aethiops, P. hamadryas and anubis, and Cebus capucinus. © 2015 John Wiley & Sons A/S.

Loading Research Institute of Medical Primatology collaborators
Loading Research Institute of Medical Primatology collaborators