Research Institute of Medical Climatology and Rehabilitation Treatment

Vladivostok, Russia

Research Institute of Medical Climatology and Rehabilitation Treatment

Vladivostok, Russia
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Barabash E.Y.,Research Institute of Medical Climatology and Rehabilitation Treatment | Kalinina E.P.,Research Institute of Medical Climatology and Rehabilitation Treatment | Gvozdenko T.A.,Research Institute of Medical Climatology and Rehabilitation Treatment | Denisenko Y.K.,Research Institute of Medical Climatology and Rehabilitation Treatment | And 3 more authors.
Medical Immunology (Russia) | Year: 2017

A control group included seventeen conditionally healthy people (Group 1). Eighty-eight patients with proven bronchial asthma (BA) at the age of 22 to 48 were enrolled into the study. I.e., Group 2 included nine patients with well-controlled BA. Group 3 included persons with partially controlled BA (n=79). There were 8 people with easily treated BA in group 2, and 57 such cases in Group 3. The levels of interleukins (IL- 4, IL-10, IL-17A), interferon-γ (IFNγ), and tumor-α necrosis factor (TNFα) were monitored by means of flow cytometry technique. The parameters of cellular immunity were registered by flow cytofluorimetry assays. Phagocytosis indicators were studied by means of D. Mayansky method, metabolic activity of neutrophils, by the B.Park method, as modified by E.Shmelev. Evaluation of cellular immunity did not reveal statistically significant differences for distinct CD subpopulations between healthy controls and BA patients. The patients with controlled and partially controlled BA exhibited some changes in cytokine concentrations, i.e., increased IL-4, IL-17., IL-10 and TNFα levels; changes in phagocytosis and oxygen dependent bactericidal activities of neutrophils. We have revealed higher concentrations of IL-4, IL-17.,in the less controlled BA (group 3) , as compared with group 2. TNFα induction remained at significantly higher level in both groups of BA patients, exceeding mean control values by 2.3 times. The degree of IL-10 production in group 2 with controlled BA was significantly higher than in group with partial disease control (group 3, p < 0.001), thus suggesting application of IL-10 levels as an index of active inflammation control. Patients with BA (groups 2, 3) exhibited a decrease of basal IFNγ, as compared to healthy people (p < 0.001). In group 3 (partial control), this parameter was 3-fold lower than in healthy persons. Evaluation of monocyte/phagocyte functions showed statistically significant differences between BA patients and healthy persons. Functional reserve of granulocyte activity and oxidative metabolism were decreased to a similar degree in the patients with well-controlled and partially resistant BA, thus showing their independence on the quality of disease control. © 2017, SPb RAACI.


Vitkina T.I.,Research Institute of Medical Climatology and Rehabilitation Treatment | Yankova V.I.,Research Institute of Medical Climatology and Rehabilitation Treatment | Gvozdenko T.A.,Research Institute of Medical Climatology and Rehabilitation Treatment | Kuznetsov V.L.,RAS Boreskov Institute of Catalysis | And 10 more authors.
Food and Chemical Toxicology | Year: 2016

The impact of two types of multi-walled carbon nanotubes (MWCNTs) (12-14 nm) with different content of metallic impurities (purified and unpurified nanotubes) on peroxidation processes, the status of immune cells in healthy volunteers and gene expression combined to pathway analysis was studied in vitro. From the study it was shown that the main mechanism of action for both types of MWCNTs is induction of oxidative stress, the intensity of which is directly related to the amount of metallic impurities. Unpurified MWCNTs produced twice as high levels of oxidation than the purified CNTs inducing thus more intense mitochondrial dysfunction. All the above were also verified by gene expression analysis of 2 different human cellular cultures (lung epithelium and keratinoma cells) and the respective pathway analysis; modulation of genes activating the NFkB pathway is associated to inflammatory responses. This may cause a perturbation in the IL-6 signaling pathway in order to regulate inflammatory processes and compensate for apoptotic changes. A plausible hypothesis for the immunological effects observed in vivo, are considered as the result of the synergistic effect of systemic (mediated by cells of the routes of exposure) and local inflammation (blood cells). © 2015 Elsevier Ltd.


PubMed | RAS Boreskov Institute of Catalysis, Aristotle University of Thessaloniki, Research Institute of Medical Climatology and Rehabilitation Treatment, University of Crete and 2 more.
Type: | Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association | Year: 2016

The impact of two types of multi-walled carbon nanotubes (MWCNTs) (12-14nm) with different content of metallic impurities (purified and unpurified nanotubes) on peroxidation processes, the status of immune cells in healthy volunteers and gene expression combined to pathway analysis was studied invitro. From the study it was shown that the main mechanism of action for both types of MWCNTs is induction of oxidative stress, the intensity of which is directly related to the amount of metallic impurities. Unpurified MWCNTs produced twice as high levels of oxidation than the purified CNTs inducing thus more intense mitochondrial dysfunction. All the above were also verified by gene expression analysis of 2 different human cellular cultures (lung epithelium and keratinoma cells) and the respective pathway analysis; modulation of genes activating the NFkB pathway is associated to inflammatory responses. This may cause a perturbation in the IL-6 signaling pathway in order to regulate inflammatory processes and compensate for apoptotic changes. A plausible hypothesis for the immunological effects observed invivo, are considered as the result of the synergistic effect of systemic (mediated by cells of the routes of exposure) and local inflammation (blood cells).


Veremchuk L.V.,Research Institute of Medical Climatology and Rehabilitation Treatment | Yankova V.I.,Research Institute of Medical Climatology and Rehabilitation Treatment | Vitkina T.I.,Research Institute of Medical Climatology and Rehabilitation Treatment | Nazarenko A.V.,Research Institute of Medical Climatology and Rehabilitation Treatment | Golokhvast K.S.,Far Eastern Federal University
Asian Pacific Journal of Tropical Biomedicine | Year: 2016

Objective: To study the mechanism of formation of air quality and to determine the impact of the studied factors on asthma morbidity in Vladivostok. Methods: The evaluation of air pollution in Vladivostok was done using long-term (2008-2012) monitoring data (temperature, humidity, atmospheric pressure, wind speed, etc.). The levels of suspended particulate matter, nitrogen and sulfur dioxide, carbon monoxide, ammonia, formaldehyde (mg/m3) in six stationary observation posts were assessed. We studied the aerosol suspensions of solid particles, which were collected during snowfall from precipitation (snow) and air in 14 districts with different levels of anthropogenic impact. Melted snow was analyzed on laser granulometry. The impact of air pollution on the distribution of asthma morbidity was evaluated in various age groups by data of federal statistical observation obtained from 8 adults and 7 children municipal clinics in Vladivostok (2008-2012). Results: The content of suspended particulate components of pollution remained more stable, due to the features of atmospheric circulation, rugged terrain and residential development. The nano- and micro-sized particles (0-50 μm), which can absorb highly toxic metals, prevail in dust aerosols. These respirable fractions of particles, even in small doses, can contribute to the increase in asthma morbidity in the city. Conclusions: We determined that asthma morbidity depends from general air pollution (in the range of 18.3%). It was detected that the highest age-specific dependence is associated with the content of particulate matter, carbon monoxide and nitrogen dioxide in air. © 2015 Hainan Medical University.

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