Saint Petersburg, Russia
Saint Petersburg, Russia

Time filter

Source Type

Abzianidze V.V.,Research Institute of Hygiene | Bolshakova K.P.,Saint Petersburg State University | Prokofieva D.S.,Research Institute of Hygiene | Berestetskiy A.O.,All Russian Institute of Plant Protection | And 2 more authors.
Mendeleev Communications | Year: 2017

The herein synthesized 7-(4-methylphenyl)thiomethyl and 7-morpholylmethyl derivatives of natural phaeosphaeride A have lower cytotoxic activity towards the human lung adenocarcinoma A549 cell line compared to phaeosphaeride A. The presence of exocyclic C=C bond and N–OMe group in such compounds is suggested to be a factor in their antitumor activity. © 2017


Sobolev V.E.,Research Institute of Hygiene | Jenkins R.O.,De Montfort University | Goncharov N.V.,Research Institute of Hygiene | Goncharov N.V.,RAS Sechenov Institute of Evolutionary Physiology and Biochemistry
Experimental and Toxicologic Pathology | Year: 2017

Glycosaminoglycans (GAGs) in the urine of Wistar rats, and on the surface of the epithelium and lamina propria of the bladder, were quantitatively assessed before and after acute intoxication with paraoxon or cyclophosphamide. Paraoxon was administered subcutaneously (s.c.) twice with an interval of 1. h to a final dose of 275. mg/kg; cyclophosphamide was administered intraperitoneally (i.p.) with a single dose of 100. mg/kg or to a final dose of 240. mg/kg (three times per 80. mg/kg every 12. h). GAGs on the surface of the epithelium and lamina propria of the urinary bladder of rats were quantitatively determined by Alcian blue dye staining. GAGs in the urine were determined spectrophotometrically with 1-9-dimethyl methylene blue. By 48-96. h after intoxication with either paraoxon or cyclophosphamide, statistically significant increases in the concentration of GAGs were obtained both for the tissues of the bladder and the urine of rats. Cyclophosphamide, in contrast to paraoxon, caused the development of hemorrhagic cystitis in the animals. The synthesis of GAGs is considered to be compensatory response to the toxic xenobiotics. © 2017 Elsevier GmbH.


Goncharov N.V.,RAS Sechenov Institute of Evolutionary Physiology and Biochemistry | Belinskaya D.A.,RAS Sechenov Institute of Evolutionary Physiology and Biochemistry | Razygraev A.V.,Do Ott Research Institute Of Obstetrics And Gynecology | Ukolov A.I.,Research Institute of Hygiene
Russian Journal of Bioorganic Chemistry | Year: 2015

Albumin molecules, unlike molecules of many other plasma proteins, are not covered with a carbohydrate shell. Albumin plays a crucial role in maintaining colloid osmotic blood pressure and can bind and transport various endogenous and exogenous molecules. The enzymatic activity of albumin, the existence and the role of which most researchers are still skeptical to accept, is our main interest. In this review, a history of the issue is traced, with particular attention paid to the esterase activity of albumin. The kinetic and thermodynamic characteristics of the interaction of albumin with some substrates are adduced, and the possibility of attributing albumin to certain groups of Enzyme Nomenclature is considered. © 2015 Pleiades Publishing, Ltd.


Prokofieva D.S.,Research Institute of Hygiene | Goncharov N.V.,RAS Sechenov Institute of Evolutionary Physiology and Biochemistry
Tsitologiya | Year: 2014

Effects of biogenic and abiogenic disulphides on viability of human umbilical vein endothelial cells in culture has been investigated using three methods: the neutral red uptake assay, quantification of intracellular ATP, and modifications of Mosmann method, the essence of which is the reduction of tetrazolium salts, MTT and MTS, by cells. 2,2'-dithio-bis(N,N-diethyl)ethanamine (DS) was used as an abiogenic disulphide. As for biogenic disulphides, we used GSSG and garlic oil (GO), the principal component of which is diallyl disulphide (DADS). It has been found that DS and GO have a similar cytotoxic effect upon the endothelial cells (EC50 ~ 0.6 mM). GSSG in concentrations up to 1 mM did not effect the viability of endothelial cells. It has been demonstrated for the first time that DS and GO can serve as mediators of plasma membrane oxidoreductase activity, tetrazolium salts being as the substrate; this may cause false-negative effect. Thus, the Mosmann method has serious limitations when testing the cytotoxicity of disulphides, though can be used in studying the mechanism of action of disulphides.


Prokofieva D.S.,Research Institute of Hygiene | Goncharov N.G.,RAS Sechenov Institute of Evolutionary Physiology and Biochemistry
Cell and Tissue Biology | Year: 2014

Effects of biogenic and abiogenic disulfides on viability of human umbilical vein endothelial cells in culture were studied using three methods: inclusion of neutral red (NR) into cells, quantification of intracellular ATP, and modifications of the Mosmann method (1983), the essence of which consists in reduction of tetrazolim salts—MTT and MTS—by cells (Mosmann, 1983). 2,2′-Dithio-bis(N,N-diethyl)dietanamine (DS) was used as an abiogenic sulfide. As for biogenic disulfides, we used oxydized glutathione (GSSG) and garlic oil (GO), the major component of which is diallyl disulfide (DADS). It has been established that DS and GO have a compatible cytotoxic effect (ES50amounts to ∼0.6 mM), whereas GSSG at a concentration up to 1 mM had no effect on the viability of endothelial cells. It has been demonstrated for the first time that DS and GO can serve as mediators of plasma-membrane oxidoreductase activity, with tetrazolium salts being used as the substrate, which may cause a false-negative effect. In this connection, Mosmann’s method has serious limitations in evaluating the effect of disulfides on cell viability, although it can be used when studying the mechanism of their action. © 2014, Pleiades Publishing, Ltd.


Belinskaya D.A.,RAS Sechenov Institute of Evolutionary Physiology and Biochemistry | Shmurak V.I.,Research Institute of Hygiene | Prokofieva D.S.,Research Institute of Hygiene | Goncharov N.V.,RAS Sechenov Institute of Evolutionary Physiology and Biochemistry
Russian Journal of Bioorganic Chemistry | Year: 2014

Albumin is known to be able to interact with organophosphorus compounds (OPCs), but neither amino acid residues of albumin that are responsible for this interaction nor the nature of the forming bonds have been finally established. Catalytic and pseudocatalytic functions of albumin are under consideration. Possible sites of interaction of albumin with soman have been elucidated by the methods of molecular modeling. Structures of the soman-albumin complexes have been determined by molecular docking. Stability of the obtained complexes has been evaluated by the method of molecular dynamics. The chemical bond between soman and the tyrosine-411 residue has been found to form only after deprotonation of the latter. The tyrosine-150 residue of albumin binds soman more effectively than tyrosine-411, and the tyrosine-150-deprotonation does not determine the efficacy of the binding (sorption) of soman, but affects the stability of the formed bound. It was proposed that the albumin residues of tyrosine-150 and serine-193 could serve as sites of the catalytic interaction with soman. We hypothesized that the deprotonation of an amino acid residue in one albumin site influenced initiation of the ligand binding in the other albumin site (allosteric albumin regulation). © 2014 Pleiades Publishing, Ltd.


PubMed | Research Institute of Hygiene and RAS Institute of Macromolecular Compounds
Type: | Journal: Journal of microencapsulation | Year: 2016

The regularities of release of therapeutic antioxidant enzyme superoxide dismutase (SOD) from various alginate-based delivery systems (DS) into simulated gastric and intestinal fluids were determined. The following systems were used: Ca-alginate granules (AG) prepared by various methods, porous carbonate cores with multilayer polyelectrolyte coating as well as the new two-level DS (Ca-AG containing carbonate cores loaded with proteins). The influence of the method of granule preparation, composition of gelation bath and ionic composition of the simulated fluids on release profiles of the protein from different DS was revealed. SEM images demonstrated changes in DS structures in various simulated fluids. The correlation between these changes and in vitro protein release was shown. The comparison of enzymatic activity of SOD encapsulated in DS of various configurations (including the systems containing different peptidase inhibitors) was made. The efficiency of protection of SOD activity in simulated intestinal fluid with trypsin was demonstrated.


Prokofieva D.S.,Research Institute of Hygiene | Jenkins R.O.,De Montfort University | Goncharov N.V.,Research Institute of Hygiene
Analytical Biochemistry | Year: 2012

Two microplate spectroscopic methods for determination of organophosphates, based on inhibition of acetylcholinesterase (AChE) activity, were further improved and evaluated for determination of the chemical weapon agent Russian VX (RVX) in aqueous solutions. The linear range of the Hestrin method (74.8-1120 pM) was 3.1-fold wider than that of the Ellman method (37.4-374 pM). Limits of detection and quantification of RVX for both methods were below the maximal allowable concentration of RVX in water-soluble washouts. One of the early products of RVX hydrolysis, N,N-diethylaminoethanethiol, like reduced glutathione, caused false negative results in the Ellman method at concentrations exceeding 10 μM; individual blanks were necessary to eliminate the effect. The Hestrin method showed greater specificity (∼3 orders of magnitude) for analysis of samples containing mercaptans. A major product of RVX degradation, 2,2′-dithiobis(N,N-diethylethanamine), caused significant inhibition of AChE at concentrations of ≥0.1 mM (P < 0.01) and had a false positive effect at higher concentrations (≥2 mM). For environmental monitoring of RVX, the method based on Hestrin is preferred over that based on Ellman, principally because the former method was less sensitive to interference from major admixtures and did not give rise to potentially dangerous false negative results. © 2011 Elsevier Inc. All rights reserved.


Prokofieva D.S.,Research Institute of Hygiene | Voitenko N.G.,Research Institute of Hygiene | Gustyleva L.K.,Research Institute of Hygiene | Babakov V.N.,Research Institute of Hygiene | And 3 more authors.
Journal of Environmental Monitoring | Year: 2010

Two microplate spectroscopic methods for determination of organophosphates, based on inhibition of acetylcholinesterase activity, have been elaborated and evaluated for determination of the chemical weapon agent soman. The principal difference between the methods is that one measures reaction substrate concentration (elaborated from Hestrin), while the other measures reaction product (elaborated from Ellman). The linear ranges of the two methods were found to be similar. Although the limit of quantification was lower for the Ellman method (110 pM), the sensitivity coefficient was in favor of the Hestrin method (1.55-fold higher). The effects of the main soman hydrolysis products were consistent for the two methods: both methylphosphonic acid and pinacolyl methylphosphonic acid did not inhibit acetylcholinesterase activity. The main components of decontaminating solutions showed differential effects: while monoethanolamine had no influence upon results obtained by either method, hydrogen peroxide interfered with the Ellman method at far lower concentrations than with the Hestrin method. In practical applications involving samples containing hydrogen peroxide, the method based on Hestrin should be regarded as much more specific for OP determination than the Ellman method. © 2010 The Royal Society of Chemistry.


PubMed | Research Institute of Hygiene
Type: Journal Article | Journal: Analytical biochemistry | Year: 2012

Two microplate spectroscopic methods for determination of organophosphates, based on inhibition of acetylcholinesterase (AChE) activity, were further improved and evaluated for determination of the chemical weapon agent Russian VX (RVX) in aqueous solutions. The linear range of the Hestrin method (74.8-1120 pM) was 3.1-fold wider than that of the Ellman method (37.4-374 pM). Limits of detection and quantification of RVX for both methods were below the maximal allowable concentration of RVX in water-soluble washouts. One of the early products of RVX hydrolysis, N,N-diethylaminoethanethiol, like reduced glutathione, caused false negative results in the Ellman method at concentrations exceeding 10 M; individual blanks were necessary to eliminate the effect. The Hestrin method showed greater specificity (~3 orders of magnitude) for analysis of samples containing mercaptans. A major product of RVX degradation, 2,2-dithiobis(N,N-diethylethanamine), caused significant inhibition of AChE at concentrations of 0.1 mM (P<0.01) and had a false positive effect at higher concentrations (2 mM). For environmental monitoring of RVX, the method based on Hestrin is preferred over that based on Ellman, principally because the former method was less sensitive to interference from major admixtures and did not give rise to potentially dangerous false negative results.

Loading Research Institute of Hygiene collaborators
Loading Research Institute of Hygiene collaborators