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PubMed | Russian National Research Medical University and Research Institute of Human Morphology
Type: Journal Article | Journal: Bulletin of experimental biology and medicine | Year: 2016

The mechanisms of proangiogenic activity of multipotent stromal cells from human umbilical cord were analyzed in vitro. The absence of secreted forms of proangiogenic growth factor VEGF-A in the culture medium conditioned by umbilical cord-derived multipotent stromal cells was shown by ELISA. However, the possibility of paracrine stimulation of cell proliferation, mobility, and directed migration of endothelial EA.hy926 cells was demonstrated by using MTT test, Transwell system, and monolayer wound modeling. The capacity of multipotent stromal cells to acquire the phenotype of endothelium-like cells was analyzed using differentiation media of three types. It was found that VEGF-A is an essential but not sufficient inductor of differentiation of umbilical cord-derived multipotent stromal cells into CD31+ cells.


PubMed | Russian National Research Medical University, Research Institute of Human Morphology and Medical Genetic Research Center
Type: Journal Article | Journal: Bulletin of experimental biology and medicine | Year: 2016

Short-term cell culturing on basement membrane matrix is a common and very convenient in vitro model of angiogenesis. We studied the possibility of interaction of multipotent stromal cells from the umbilical cord and Ea.hy926 endothelial cells on this model at the early and late periods of the experiment. Multipotent stromal cells alone and in combination with endothelial cells formed an unstable tubular network. Clusters formed after its disassembling later became the sprouting centers in co-culture of the two cell types, but not in pure culture of multipotent stromal cells. Multipotent stromal cells with CD31+ phenotype constitute the structural basis of newly formed stable 3D capillary-like network. Prolongation of the time of culturing and combination of the two in vitro models of angiogenesis (tubulogenesis and sprouting) allowed more complete assessment of the angiogenic potential of multipotent stromal cells.


Kharlamova A.S.,Research Institute of Human Morphology | Saveliev S.V.,Research Institute of Human Morphology | Protopopov A.V.,Academy of science of the Sakha Republic Yakutia | Maseko B.C.,University of Witwatersrand | And 2 more authors.
Journal of Comparative Neurology | Year: 2015

This study presents the results of an examination of the mummified brain of a pleistocene woolly mammoth (Mammuthus primigenius) recovered from the Yakutian permafrost in Siberia, Russia. This unique specimen (from 39,440-38,850 years BP) provides the rare opportunity to compare the brain morphology of this extinct species with a related extant species, the African elephant (Loxodonta africana). An anatomical description of the preserved brain of the woolly mammoth is provided, along with a series of quantitative analyses of various brain structures. These descriptions are based on visual inspection of the actual specimen as well as qualitative and quantitative comparison of computed tomography imaging data obtained for the woolly mammoth in comparison with magnetic resonance imaging data from three African elephant brains. In general, the brain of the woolly mammoth specimen examined, estimated to weigh between 4,230 and 4,340 g, showed the typical shape, size, and gross structures observed in extant elephants. Quantitative comparative analyses of various features of the brain, such as the amygdala, corpus callosum, cerebellum, and gyrnecephalic index, all indicate that the brain of the woolly mammoth specimen examined has many similarities with that of modern African elephants. The analysis provided here indicates that a specific brain type representative of the Elephantidae is likely to be a feature of this mammalian family. In addition, the extensive similarities between the woolly mammoth brain and the African elephant brain indicate that the specializations observed in the extant elephant brain are likely to have been present in the woolly mammoth. © 2015 Wiley Periodicals, Inc.


PubMed | Russian Academy of Sciences, Yakut State Agricultural Academy, Mammoth Site of Hot Springs Inc., Research Institute of Human Morphology and Yakutian Academy of Science
Type: Journal Article | Journal: Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections | Year: 2016

The paper presents the first morphological description of the internal organs of a frozen corpse of the steppe bison Bison priscus (Bojanus, 1827) from the Holocene of northern Yakutia. Necropsy revealed that most of the internal organs, including the brain, heart with the main vessels, and reproductive system were well preserved. It demonstrated that the anatomy of this bison was close to that of the genera Bos and Bison. Trauma or pathological changes in the organs were not detected. The cause of death of the bison remains unknown.


Arutyunyan I.V.,Research Institute of Human Morphology | Kananykhina E.Y.,Research Institute of Human Morphology | Makarov A.V.,Research Institute of Human Morphology
Cellular Transplantation and Tissue Engineering | Year: 2013

We present an overview of current literature data on the different roles of key proangiogenic factor VEGF-A-165' receptors in angiogenesis regulation. VEGFR2 is the major effector receptor and runs intracellular cascades that provide survival, proliferation and migration of endothelial cells, the involvement of progenitor cells, the formation and maturation of new blood vessels. VEGFR1 contrary is the main regulator of the activity of VEGF-A-165, preventing excessive angiogenic response through the mechanism of negative feedback. The article also presents data on the variability of number and ratio of VEGFR1 and VEGFR2 on the surface of different cell types, providing the fine regulation of the VEGF-A-165 pathway. © Human stem cells institute, 2013.


Yaglova N.V.,Research Institute of Human Morphology | Obernikhin S.S.,Research Institute of Human Morphology
Bulletin of Experimental Biology and Medicine | Year: 2016

Differentiation of T cells was studied in the progeny of female C57Bl/6 mice subjected to immunostimulation by administration of concanavalin A or adoptive transfer of concanavalin A-activated splenocytes. Acceleration of T cell maturation during the prepubertal period and decelerated differentiation during the pubertal and post-pubertal periods were revealed. © 2016 Springer Science+Business Media New York


Yaglova N.V.,Research Institute of Human Morphology | Yaglov V.V.,Research Institute of Human Morphology
Biomedit︠s︡inskai︠a︡ khimii︠a︡ | Year: 2016

Changes in secretion of thyroid autoantigenes and production of antithyroid autoantibodies after long-term exposure to low doses of DDT were studied. Changes in serum levels of antithyroid peroxidase antibodies and thyroid peroxidase, attributed to disruption of thyroxine production by DDT were found. Long-term exposure of rats to low doses of DDT revealed no specific impact on serum autoantibodies to all thyroid autoantigenes studied. The increase of the ratio of autoantibody/autoantigen for thyroid peroxidase and thyroglobulin was rather small and thus could not be considered as a significant symptom of thyroid autoimmunity.Abstract available from the publisher.


PubMed | Research Institute of Human Morphology and RAS Engelhardt Institute of Molecular Biology
Type: Journal Article | Journal: Tissue & cell | Year: 2016

In the pancreas of many mammals including humans, endocrine islet cells can be integrated with the nervous system components into neuro-insular complexes. The mechanism of the formation of such complexes is not clearly understood. The present study evaluated the interactions between the nervous system components, epithelial cells and endocrine cells in the human pancreas. Foetal pancreas, gestational age 19-23 weeks (13 cases) and 30-34 weeks (7 cases), were studied using double immunohistochemical labeling with neural markers (S100 protein and beta III tubulin), epithelial marker (cytokeratin 19 (CK19)) and antibodies to insulin and glucagon. We first analyse the structure of neuro-insular complexes using confocal microscopy and provide immunohistochemical evidences of the presence of endocrine cells within the ganglia or inside the nerve bundles. We showed that the nervous system components contact with the epithelial cells located in ducts or in clusters outside the ductal epithelium and form complexes with separate epithelial cells. We observed CK19-positive cells inside the ganglia and nerve bundles which were located separately or were integrated with the islets. Therefore, we conclude that neuro-insular complexes may forms as a result of integration between epithelial cells and nervous system components at the initial stages of islets formation.


PubMed | Research Institute of Human Morphology
Type: Journal Article | Journal: Bulletin of experimental biology and medicine | Year: 2016

Differentiation of T cells was studied in the progeny of female C57Bl/6 mice subjected to immunostimulation by administration of concanavalin A or adoptive transfer of concanavalin A-activated splenocytes. Acceleration of T cell maturation during the prepubertal period and decelerated differentiation during the pubertal and post-pubertal periods were revealed.


PubMed | Research Institute of Human Morphology and University of Kiel
Type: Journal Article | Journal: Arkhiv patologii | Year: 2016

to improve the immunohistochemical diagnosis of AL amyloidosis, by generating novel peptide antibodies against the variable and constant regions of the -light chains.All amyloidogenic -light chains were sought in the scientific literature and the database of the National Center for Biotechnology Information. On the basis of the findings, a chain was formed from the most common amino acid residues that were used to choose peptide regions for immunization. Four antibodies were generalized via immunization of rabbits with two peptides that corresponded to the variant or constant regions of -light chains.The specificity of the obtained antibodies was confirmed using a series of 222 biopsy specimens from 193 patients with AL, AA, transthyretin, or insulin amyloidosis. All the novel polyclonal peptide antibodies produced positive staining in cases of AL amyloidosis.The generated polyclonal peptide antibodies against the variable and constant regions of -light chains are able to improve the immunohistochemical diagnosis of amyloidosis.

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