Research Institute of Forest and Rangelands

Tehrān, Iran

Research Institute of Forest and Rangelands

Tehrān, Iran
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Hasanbarani M.,Islamic Azad University at Tehran | Sharifnia F.,Islamic Azad University at Tehran | Nejadsattari T.,Islamic Azad University at Tehran | Assadi M.,Research Institute of Forest and Rangelands
Biodiversitas | Year: 2017

Delphinium khorasanicum Sharifnia & Hasanbarani sp. nov., a species distributed in NE Iran, is described and provided with a molecular diagnosis. It is morphologically related to D. turkmenum Lipsky. The most important morphological differences between the new species and D. turkmenum are mainly in the shape of spur, plant length and epidermal patterns. This species is compared with close taxa (they are close and relative in point of view morphologically, geographically and molecular characters). Illustration and distribution map are also presented. © 2017, Society for Indonesian Biodiversity. All rights reserved.


Jamalian A.,Pasteur Institute of Iran | Jamalian A.,Islamic Azad University at Tehran | Shams-Ghahfarokhi M.,Tarbiat Modares University | Jaimand K.,Research Institute of Forest and Rangelands | And 3 more authors.
Journal de Mycologie Medicale | Year: 2012

Objective: Fungal infections are potential public health threats all over the world. In the present study, effect of Matricaria recutita flower essential oil (EO) was evaluated against medically important dermatophytes and opportunistic saprophytes using microbioassay technique. Materials and methods: Flower essential oil (EO) of M. recutita prepared by hydrodistillation was analyzed by gas chromatography/mass spectrometry (GC/MS). The effect of plant EO on the growth of pathogenic dermatophytes and opportunistic saprophytes was assessed using microbioassay technique. In the bioassay, fungi were cultured in 6-well flat-bottom microplates in presence of various concentrations of plant EO (2.5-1000. μg/mL) for 4-10. days at 28. °C. Results: A total of 14 compounds were identified in the plant oil by GC/MS accounting for 97.5% of the oil composition. The main compound identified was chamazulene (61.3%) followed by isopropyl hexadecanoate (12.7%), trans-trans-farnesol (6.9%) and E-β-farnesol (5.2%). Growth inhibition for the dermatophytes exposed to serial two-fold concentrations of plant EO (2.5 to 80. μg/mL) was reported in the range of 3.24 to 68.15% for Microsporum gypseum, 24.48 to 100% for M. canis, 11.40 to 96.65% for Trichophyton mentagrophytes, 27.79 to 100% for T. rubrum and 45.73 to 100% for T. tonsurans. M. recutita EO inhibited the growth of opportunistic saprophytes by 3.98 to 64.29% for Aspergillus flavus, 6.38 to 93.62% for A. fumigatus, 3.52 to 89.45% for A. niger, 6.38 to 77.66% for Trichoderma harzianum and 17.41 to 89.41% for Fusarium oxysporum in serial two-fold concentrations of 15.62 to 1000. μg/mL. Conclusion: Results of the present study indicate that M. recutita could be considered as a potential candidate for designing effective antifungal formulations suitable for treatment of dermatophytosis and other fungal infections. © 2012 Elsevier Masson SAS.


Rashidi F.,Islamic Azad University at Tehran | Jalili A.,Research Institute of Forest and Rangelands | Kafaki S.B.,Islamic Azad University at Tehran | Sagheb-Talebi K.,Research Institute of Forest and Rangelands | Hodgson J.,University of Sheffield
Trees - Structure and Function | Year: 2012

This study investigates responses in the leaf anatomy of Black Locust (Robinia pseudoacacia L.) to the atmospheric pollutants, SO 2, NO 2 and O 3 and climate in Tehran. The anatomical variables studied include thickness of the leaf lamina and of its main constituent tissues and the length and density of stomata. We present evidence that, in response to urban air pollution, the spongy mesophyll layer is thinner, the upper cuticle of the leaf thicker and stomatal density and the ratio of palisade parenchyma to spongy parenchyma are increased. Similar responses were also detected in relation to a climatic gradient. Stomatal density and thickness of the leaf lamina and of its mesophyll layer were all higher under warmer drier conditions. This overlap in anatomical response to two very different suites of environmental variables may reflect a functional overlap between mechanisms designed to restrict water loss in dry climates and those that minimize the uptake of toxic gases in polluted habitats. © 2011 Springer-Verlag.


Yousefzadeh S.,Tarbiat Modares University | Modarres-Sanavy S.A.M.,Tarbiat Modares University | Sefidkon F.,Research Institute of Forest and Rangelands | Asgarzadeh A.,Soil and Water Research Institute | And 2 more authors.
Food Chemistry | Year: 2013

Dragonhead is an annual, herbaceous, balm-scented and spicy aromatic member of the family Lamiaceae. We examined effects of different sources of nitrogen on the content and composition of essential oils in two genotypes of dragonhead in two regions of Iran. The sources of nitrogen used were 100% urea (70 kg N ha-1), 75% urea (52.5 kg N ha-1) + 25% Azocompost (3.85 ton ha-1), 50% urea (35 kg N ha-1) + 50% Azocompost (7.77 ton ha-1), 25% urea (17.5 kg N ha-1) + 75% Azocompost (11.55 ton ha-1), and 100% Azocompost (15.55 ton ha-1). Optimal yield and content of essential oil at both locations for both genotypes were obtained by applying 50% urea + 50% Azocompost. Geraniol, geranial, and geranyl acetate were the most abundant compounds. For both genotypes and both locations, application of 50% urea + 50% Azocompost increased levels of geraniol and geranial, and application of Azocompost alone increased levels of geranyl acetate. Overall, we conclude that the application of 50% urea with 50% Azocompost is recommended for optimising the content and composition of essential oils in dragonhead. © 2012 Elsevier Ltd. All rights reserved.


Mirza M.,Research Institute of Forest and Rangelands | Navaei M.N.,Research Institute of Forest and Rangelands | Mahnesaee A.,University of Yazd
Journal of Essential Oil-Bearing Plants | Year: 2015

The volatile constituents of the oil extracted from the root, leaf and seed of Cleome quinquenervia DC were isolated through a hydro distillation method and then analyzed by GC and GC-MS.. Root, leaf and seed were found to contain 15, 52, and 42 components respectively. The major components of leaf oil were found to be β-pinene (31 %), α-pinene (26.1 %), trans-pinocarvyl acetate (6.6 %). The root oil constituents were characterized by high amounts of α-eudesmol (29 %), β-eudesmol (27.5 %) and γ-eudesmol (13 %). The main constituents of the seed oil were shown to trans-pinocarvyl acetate (12.5 %), β-eudesmol (10.8 %) and β- pinene (10.8 %), α-eudesmol (9.6 %). © 2015, Har Krishan Bhalla & Sons.


Kanani M.R.,University of Isfahan | Kanani M.R.,Shahid Beheshti University | Rahiminejad M.R.,University of Isfahan | Sonboli A.,Shahid Beheshti University | And 4 more authors.
Chemistry and Biodiversity | Year: 2011

To evaluate the chemotaxonomic significance of the essential oils of 23 populations of 18 Iranian Ferula species, the chemical composition of the oils was investigated by GC/FID and GC/MS. Altogether, 84 constituents, representing 81.3-99.7% of the total composition of the oils, have been identified. The composition of six species of the genus, i.e., F. oopoda, F. foetida, F. behboudiana, F. diversivittata, F. galbaniflua, and F. hezarlalehzarica, has been reported for the first time. The main constituents identified were a-terpinyl acetate (73.3%), 2,3,4-trimethylthiophene (2; 49.0%), sabinene (75.3%), verbenone (5; 69.4%), β-pinene (59.0-66.3%), and (Z)-β-ocimene (41.7%). Cluster analysis (CA) of the percentage content of the essential oil components of the Ferula species resulted in the characterization of four groups, i.e., taxa containing either i) monoterpene hydrocarbons, ii) oxygenated monoterpenes, iii) organosulfur compounds, or iv) monoterpene, sesquiterpene, and aliphatic hydrocarbons as the principal classes of compounds. Based on the results obtained, the chemical independence of F. hirtella from F. szowitsiana and of F. galbaniflua from F. gummosa at the specific level was concluded and their positions as distinct species were confirmed. The chemotaxonomic relationships among the representatives of the genus Ferula have been discussed in detail. © 2011 Verlag Helvetica Chimica Acta AG.


Navaei M.N.,Research Institute of Forest and Rangelands | Mirza M.,Research Institute of Forest and Rangelands
Journal of Essential Oil-Bearing Plants | Year: 2016

Abstract: The volatile constituents of the oil extracted from the leaves and flowers of Nepeta haussknechtii Bornm. were isolated through a hydrodistillation method and then analyzed by GC and GC-MS. The identified components accounted for 98.9%, 99%, of the leaves and flowers, The leaf oil was found to contain 26 components and the flower oil contained 25. The major constituents of the leaf oil were germacrene D (32%), and caryophyllene oxide (16.5%), and the main components of the flower oil were germacrene D (41.5%),, E-caryophyllene (20.2%) and thymol (7.7%). © 2016 Har Krishan Bhalla & Sons.


Tolouee M.,Islamic Azad University at Karaj | Alinezhad S.,Institute of Scientific Applied Higher Education of Jahad E Agriculture | Saberi R.,Institute of Scientific Applied Higher Education of Jahad E Agriculture | Eslamifar A.,Pasteur Institute of Iran | And 7 more authors.
International Journal of Food Microbiology | Year: 2010

The antifungal activity of Matricaria chamomilla L. flower essential oil was evaluated against Aspergillus niger with the emphasis on the plant's mode of action at the electron microscopy level. A total of 21 compounds were identified in the plant oil using gas chromatography/mass spectrometry (GC/MS) accounting for 92.86% of the oil composition. The main compounds identified were -bisabolol (56.86%), trans-trans-farnesol (15.64%), cis-farnesene (7.12%), guaiazulene (4.24%), -cubebene (2.69%), -bisabolol oxide A (2.19%) and chamazulene (2.18%). In the bioassay, A. niger was cultured on Potato Dextrose Broth medium in 6-well microplates in the presence of serial two fold concentrations of plant oil (15.62 to 1000. μg/mL) for 96. h at 28. °C. Based on the results obtained, A. niger growth was inhibited dose dependently with a maximum of 92.50% at the highest oil concentration. A marked retardation in conidial production by the fungus was noticed in relation to the inhibition of hyphal growth. The main changes of hyphae observed by transmission electron microscopy were disruption of cytoplasmic membranes and intracellular organelles, detachment of plasma membrane from the cell wall, cytoplasm depletion, and complete disorganization of hyphal compartments. In scanning electron microscopy, swelling and deformation of hyphal tips, formation of short branches, and collapse of entire hyphae were the major changes observed. Morphological alterations might be due to the effect on cell permeability through direct interaction of M. chamomilla essential oil with the fungal plasma membrane. These findings indicate the potential of M. chamomilla L. essential oil in preventing fungal contamination and subsequent deterioration of stored food and other susceptible materials. © 2010 Elsevier B.V.


Rahiminejad M.R.,University of Isfahan | Mozaffarian V.,Research Institute of Forest and Rangelands | Montazerolghaem S.,University of Isfahan
Botanical Journal of the Linnean Society | Year: 2010

The taxonomic status of Centaurea section Acrocentron in Iran is briefly reviewed. A critical study of 251 herbarium specimens, and an additional 37 accessions collected specifically for this research, led to the discovery of a new variety, Centaurea irritans var. longispinosa Montazerolghaem, and the revival of another, C. persica var. subinermis, a taxon previously treated as a synonym for about 25 years. A key to all Iranian species of Centaurea section Acrocentron is provided. © 2010 The Linnean Society of London, Botanical Journal of the Linnean Society, 2010, 163, 99-106. © 2010 The Linnean Society of London.


Hamdi S.M.M.,Islamic Azad University at Garmsar | Assadi M.,Research Institute of Forest and Rangelands | Segarra-Moragues J.G.,University of Zaragoza
Feddes Repertorium | Year: 2010

Pollen analyses of 12 Iranian species of Typha L. were conducted through light microscopy and scanning electron microscopy. Among the twelve studied species, we found that five species, T. caspica, T. latifolia, T. lugdunensis, T. minima and T. shuttleworthii, present pollen in tetrads, whereas the remaining seven species, T. angustifolia, T. azerbaijanensis, T. domingensis, T. grossheimii, T. kalatensis, T. laxmanii and T. tourcomanica, have pollen in monads. The exine of Typha species is mostly reticulate although a few species present perforate-microreticulate and rugulate exine. Our analyses revealed that using a combination of light microscopy and scanning eletron microscopy characters the identification of species is feasible. A diagnostic key based on palynological traits is presented for all studied taxa. © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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