Research Institute of Fish Culture and Hydrobiology

Czech Republic

Research Institute of Fish Culture and Hydrobiology

Czech Republic
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Sampels S.,Institute of Aquaculture and Protection of Waters | Sampels S.,Swedish University of Agricultural Sciences | Kroupova H.K.,Research Institute of fish Culture and Hydrobiology | Linhartova P.,Institute of Aquaculture and Protection of Waters | Linhartova P.,Masaryk University
Toxicology in Vitro | Year: 2017

The intake of cadmium contaminated fish was mimicked by incubating human hepatoblastoma cells (Cell line HepG2) with a combination of different levels of cadmium (0–5 μM) plus the n-3 fatty acids docosahexaenoic acid and eicosapentaenoic acid, which are typical for fish. Uptake of cadmium, iron, copper and zinc was measured by ICP-MS. In addition mRNA expression of two metallothioneins (mt1 g and mt1 m) was evaluated by real-time PCR. The obtained data shows that the presence of cadmium increases the uptake of iron and zinc into the HepG2 cells while the uptake of copper remains unaffected. The presence of the chosen fatty acids did not affect the uptake of either cadmium or iron, zinc and copper. The presence of already 1 μM cadmium increased the mRNA expression of mt1 g and mt1 m significantly, while the fatty acids did not interfere with the effect of cadmium. © 2017 Elsevier Ltd

Golpour A.,Research Institute of Fish Culture and Hydrobiology | Esfandyari M.,Gorgan University of Agricultural Sciences and Natural Resources | Dadras H.,Islamic Azad University at Lahijan
Iranian Journal of Fisheries Sciences | Year: 2015

Motility parameters of the spermatozoa in most fish species spawning in fresh water like Rutilus kutum lasts for a short time after activation. Ovarian fluid significantly influenced sperm motility (motility duration period) and percent motility (progressive forward motile sperm). Both of these variables generally increased as the concentration of ovarian fluid increased from 33% to 50%, respectively. It is concluded that ovarian fluid enhances sperm movement in this species at appropriate level and thus has the potential to influence fertilization capacity.

Stara A.,Research Institute of Fish Culture and Hydrobiology | Steinbach C.,Research Institute of Fish Culture and Hydrobiology | Wlasow T.,University of Warmia and Mazury | Gomulka P.,University of Warmia and Mazury | And 3 more authors.
Neuroendocrinology Letters | Year: 2013

OBJECTIVES: The aim of this study was to assess the effect of pesticide Fury 10 EW, containing zeta-cypermethrin 100 g.l-1, on common carp (Cyprinus carpio L.). DESIGN: The toxicity tests were performed on common carp according to OECD 203 methodologies. The common carp were exposed to Fury 10 EW at concentrations, 5, 7, 10, 50 and 100 μg.l- for 96 h and compared to common carp in a non-treated control group. Acute toxicity tests were detected value 96hLC50=13.8 μg.l-1. On the basis of the results was assessed the effect on the hematological profile, oxidative stress parameters, and antioxidants biomarkers in tissues, in another acute test. RESULTS: The observed 96hLC50 value of Fury 10 EW was 13.8 μg.l-1. A significantly lower large lymphocyte and monocyte count, and a significantly greater number of segmented eosinophil granulocyte and higher hematocrit was found in the pesticide-exposed common carp compared to controls. Oxidative damage was not detected in the experimental common carp, however there were significant differences from control in superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) in tissue after acute exposure to 13.8 μg.l-1 Fury 10 EW. CONCLUSIONS: Zeta-cypermethrin as Fury 10 EW was classified as a substance highly toxic to fish. The hematological profile well oxidative stress parameters and antioxidant defensive systems provide important information about the internal environment of organisms. There is a lack of experimental results about the effects of zeta-cypermethrin on fish in the literature. ©2013 Neuroendocrinology Letters.

PubMed | Research Institute of Fish Culture and Hydrobiology
Type: Journal Article | Journal: TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik | Year: 2013

The correspondence between increased numbers of both chromosomal and nuclear NORs and artificially induced triploidy in three fish species (rainbow trout, Oncorhynchus mykiss; common carp, Cyprinus carpio; and tench, Tinea tinea) has been confirmed by CMA3 fluorescence and Ag-staining. The frequencies of cell nuclei with one, two and three active NORs, as revealed by Ag-staining, has been analyzed statistically to find the minimum cell number which verifies the increased ploidy level. A minimum sample size of about 80 cells exhibiting three active NORs is sufficient to confirm triploidy in all three species and may be of use for categorising other ploidy-manipulated fish species.

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