Research Institute of Clinical and Experimental Lymphology

Siberian, Russia

Research Institute of Clinical and Experimental Lymphology

Siberian, Russia

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Konenkov V.I.,Research Institute of Clinical and Experimental Lymphology | Klimontov V.V.,Research Institute of Clinical and Experimental Lymphology
Diabetes Mellitus | Year: 2014

Genetic and cellular techniques emerge as promising modalities for the treatment of diabetic foot syndrome. Two patient groups potentially to benefit most from these novel methods are patients with critical lower limb ischemia (CLLI) in whom angiosurgery is not indicated, and patients with trophic ulcers resistant to conventional therapy. A series of clinical trials has shown positive effects of transferring VEGF, HIF-1, FGF, PDGF, HGF and certain other growth factor genes to stimulate blood vessel formation and healing of diabetic ulcers. Autologous transplantation of mononuclear bone marrow and peripheral blood cells, endothelial progenitor cells, mesenchymal stem cells and stromal cell of the adipose tissue has also demonstrated its clinical potential in patients with diabetes mellitus and CLLI. Randomized clinical trials report beneficial effects of gene and cell therapy on such surrogate endpoints as ischemic index, rest pain and ulcer healing, though data on amputation rates is controversial. Further studies are necessary to determine optimal dosage and route of administration of biological agents and predictors of their efficacy, as well as long-term safety of these novel treatment modalities.


Klimontov V.V.,Research Institute of Clinical and Experimental Lymphology | Myakina N.E.,Research Institute of Clinical and Experimental Lymphology
Diabetes Mellitus | Year: 2014

Glargine became the first long-acting insulin analogue. Glargine was designed to meet basal insulin requirements throughout the day with a single injection. Pharmacokinetics of insulin glargine is characterized by biotransformation into metabolites M1 and M2 that transforms the B chain of glargine so it is similar to the B chain of human insulin. Plasma concentrations of active M1 and M2 metabolites have no pronounced peaks during the day, resulting in lower glucose variability and hypoglycaemia risk when compared with NPH insulin. The metabolic activities of M1 and M2 metabolites are similar to the effect of glargine, whereas the mitogenic effects of these metabolites do not exceed the effect of human insulin. Insulin glargine shows a higher affinity for the insulin-like growth factor-1 (IGF-1) receptor when compared with human insulin. Glargine has no proliferative effect in vivo owing to its rapid conversion into metabolites. Pharmacokinetic and pharmacodynamic variability of glargine is comparable to other insulins. These characteristics are important for the clinical efficacy and safety of glargine.


PubMed | Research Institute of Clinical and Experimental Lymphology
Type: Journal Article | Journal: Terapevticheskii arkhiv | Year: 2016

To estimate the relationships between the serum concentrations of acute-phase proteins (APPs) and adipocytokines, body composition (BC), and blood glucose (BG) fluctuations in women with type 2 diabetes mellitus (T2DM).A total of 165 women with T2DM and 22 with a normal body mass index (BMI) at the age of 40 to 70 years were examined. The concentrations of high-sensitivity C-reactive protein (hs-CRP) and acid 1-glycoprotein (1-AGP) were determined by ELISA. The levels of interleukins 6, 8, and 18 (IL-6, IL-8, IL-18), tumor necrosis factor- (TNF-), and plasminogen activator inhibitor type 1 (PAI-1) were measured by a multiplex analysis. Dual energy X-ray absorptiometry was used to estimate BC parameters. BG fluctuations were estimated via continuous glucose monitoring.The levels of hs-CRP, 1-AGP, IL-6, IL-8, IL-18, TNF-, and PAI-1 were significantly higher in the obese women with T2DM than those in the control group. In the diabetic normal weight women, only hs-CRP, 1-AGP, and IL-8 concentrations exceeded those in the controls. The level of hs-CRP (other than 1-AGP) correlated positively with BMI, the mass of adipose tissue, body trunk (android), and gynoid fats. A multivariate regression analysis showed that adipose tissue mass and trunk fat proportion were independent predictors of hs-CRP levels. The concentrations of IL-6, IL-8, IL-18, PAI-1, and TNF- correlated positively with waist-to-hip ratio, but demonstrated no associations with BMI and BC. Only the serum 1-AGP level showed a positive association with mean BG and its variability parameters.In the women with T2DM, the serum concentrations of APPs and adipocytokines correlate differently with the mass of adipose tissue, its distribution, and BG fluctuations. The findings indicate the multifactorial genesis of chronic inflammation in these patients.


PubMed | RAS Institute of Cytology and Genetics and Research Institute of Clinical and Experimental Lymphology
Type: Journal Article | Journal: Bulletin of experimental biology and medicine | Year: 2016

Effect of the dipeptidyl peptidase-4 inhibitor linagliptin on structural manifestations of diabetic nephropathy was studied in BKS.Cg-Dock7m+/+Leprdb/J mice (experimental model of type 2 diabetes mellitus). Linagliptin (10 mg/kg per day) or vehicle was administered by gavage over 8 weeks. Mesangial expansion, thickening of the basement membrane in glomerular capillaries and proximal tubules, and retraction of cytopodia were less pronounced in mice receiving linagliptin. The protective effect of linagliptin on the kidney structure was not associated with its hypoglycemic action.


PubMed | Research Institute of Clinical and Experimental Lymphology and Research Institute of Physiology and Fundamental Medicine
Type: Journal Article | Journal: Bulletin of experimental biology and medicine | Year: 2016

We studied the effect of a biomedical cell product (bone marrow multipotent mesenchymal stromal cells and products secreted by these cells in conditioned medium) on subpopulation composition of lymphoid cells of central and peripheral lymphoid organs of Wistar rats under normal conditions. Changes in the subpopulation composition of lymphoid organs depended on the route of administration of biomedical cell product.


PubMed | RAS Institute of Cytology and Genetics and Research Institute of Clinical and Experimental Lymphology
Type: Journal Article | Journal: Bulletin of experimental biology and medicine | Year: 2016

We studied the effect 24-h illumination on quantitative and qualitative parameters of the bone marrow cells in Wistar rats. It was shown that desynchronosis reduced the release of nucleated cells from the femoral bone, while melatonin weakened this effect. The number of bone marrow mesenchymal stromal cells was resistant to circadian rhythm disturbances, while proliferation depended on glucose concentration in the medium.


PubMed | Institute of Fundamental and Clinical Immunology, Research Institute of Clinical and Experimental Lymphology and Research Institute of Physiology and Fundamental Medicine
Type: Journal Article | Journal: Bulletin of experimental biology and medicine | Year: 2016

The effects of anti-CD208 antibodies (mannose receptor) on functional characteristics of peritoneal macrophages were studied in intact mice and mice with lipidemia induced by poloxamer-407. Lipidemia was associated with suppression of phagocytosis and increase in spontaneous proliferative potential and NO production by macrophages. Anti-CD206 antibodies suppressed NO production by macrophages in mice with lipidemia.


PubMed | Research Institute of Clinical and Experimental Lymphology
Type: Journal Article | Journal: Bulletin of experimental biology and medicine | Year: 2016

We studied the effect of officinal forms and nanoparticles of lithium carbonate and lithium citrate on proliferative activity of hepatoma-29 cells. Lithium carbonate nanoparticles suppressed proliferation of hepatoma-29 cells in lower concentrations than officinal form of this salt. The antiproliferative effect of lithium salts i activation of apoptosis and arrest of hepatoma-29 cells in the G2/M phase of the cell cycle.


PubMed | Research Institute of Clinical and Experimental Lymphology
Type: Journal Article | Journal: Terapevticheskii arkhiv | Year: 2016

To study the promoter regions of the matrix metalloproteinase (MMP)2, MMP3, and MMP9 genes to assess their associations with the risk of rheumatoid arthritis (RA) and with the types of its clinical course in women.162 female patients with RA and 329 women without this condition were examined. Polymorphisms in the gene promoter region for MMP2 (-1306 ), MMP3 (-1171 5A6), and MMP9 (-1562 ) were studied. Genotyping was carried out using the restriction fragment length polymorphism method.In the RA group, the -1306TT genotype of MMP2 was significantly more frequently encountered and the 6A6A genotype was less frequently seen. In the seropositive RA group, the frequency of the -1306 genotype of MMP2 was significantly higher than that in the healthy individuals. The significant differences shown for the entire group of patients with RA were preserved when they were divided into groups according to the presence or absence of rheumatoid nodules. Furthermore, the frequency of the homozygous -1306 genotypes of MMP2 was higher in both groups than in the healthy individuals.The presence of the allelic variants of the MMP genes may be one of the genetic factors that predispose to RA in women.


PubMed | Institute of Molecular Pathology and Pathomorphology, Altai State Medical University and Research Institute of Clinical and Experimental Lymphology
Type: Journal Article | Journal: Bulletin of experimental biology and medicine | Year: 2016

Morphological and ultrastructural study of the kidney was performed in rats with oxalate nephrolithiasis. Specific features of endoplasmic reticulum stress were evaluated during nephrolithiasis and treatment with -tocopherol. We observed the signs of endoplasmic reticulum stress with activation of proapoptotic pathways and injury to the cell lining in nephron tubules and collecting ducts. Ultrastructural changes were found in the organelles, nuclei, and cell membranes of epitheliocytes. A relationship was revealed between endoplasmic reticulum stress and oxidative damage, which developed at the early state of lithogenesis.

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