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Mukasheva T.,Al-Farabi Kazakh National University | Berzhanova R.,Al-Farabi Kazakh National University | Ignatova L.,Al-Farabi Kazakh National University | Omirbekova A.,Al-Farabi Kazakh National University | And 3 more authors.
Acta Biochimica Polonica | Year: 2016

In this study, 382 isolates of bacterial endophytes from tissues of plants grown in the foothills and piedmont plains of the Trans-Ili Alatau were isolated. It was found that certain strains actively produce indole-3-acetic acid (IAA) in a medium containing l-tryptophan. Among the strains studied, 26 strains (66%) showed a positive response to production of IAA. Bacteria synthesized IAA in the range of 18.6 ± 1.1 to 82.4 ± 2.3 μg/ml. IAA was synthesized more actively by Streptosporangium sp. KK1 (44.1 μg/ml), Rhodococcus sp. KK 2 (42.5 μg/ml), Streptomyces tendae KK3 (44.9 μg/ml) strains. The most active auxin's producer is a Jeotgalicoccus halotolerans BAK1 strain, whose total level of IAA production reached 82.4 μg/ml. Such strains as Streptomyces griseorubiginosis ΚΚ4, Streptomyces sp. ΚΚ5 and Jeotgalicoccus halotolerans Κ1 were found to have a significant stimulating effect which was reflected in the increase of the length of the roots of soybean and barley. As a result, 8 promising strains with fungicidal, growth-stimulating, phosphorus dissolving and enzymatic activities were selected for the further development of highly microbiological preparations for crop research.

Ignatova L.,Al-Farabi Kazakh National University | Brazhnikova Y.,Research Institute of Biology and Biotechnology Problems | Berzhanova R.,Al-Farabi Kazakh National University | Mukasheva T.,Al-Farabi Kazakh National University
Acta Biochimica Polonica | Year: 2015

The possibility of application of micromycetes (strains Penicillium bilaiae Pb14, Aureobasidium pullulans YA05 and Rhodotorula mucilaginosa YR07) to increase yields of soybean (Glycine max cv Almaty) and barley (Hordeum vulgare cv Arna) was estimated. It was shown that the most positive effect on germination energy and seed germination after seed treatment with liquid culture, supernatant and filtrate, is achieved at 1:5 dilution. In studying the influence of cell-associated and extracellular biologically active compounds of micromycetes (liquid culture and supernatant) on biometric parameters of seedlings, the maximum stimulating effect was observed in the variants with liquid culture. These strains of micromycetes were used as a bases for various compositions of preparations - application of each strain separately and application of micromycetes mixes. In microfield experiments, the increase of soybean yield ranged from 4.5 to 9.4 quintal/ha, barley - from 2.9 to 5.9 quintal/ha. A significant increase in various parameters of structure of the yield was shown in all experimental variants when compared to the control. It was found that an increase in soybean and barley yields and yield components was higher in the variant with a mix of micromycetes when compared to the separate application of each strain. The most efficient mixture was based on the mix of fungal strains (culture filtrate of P. bilaiae Pb14 diluted 1:5 + liquid cultures of A. pullulans YA05 and Rh. mucilaginosa YR07 in a 1:5 dilution).

Bari A.A.,Research Institute of Biology and Biotechnology Problems | Sagaidak A.I.,Research Institute of Biology and Biotechnology Problems | Pinskii I.V.,Research Institute of Biology and Biotechnology Problems | Orazova S.B.,Research Institute of Biology and Biotechnology Problems | Ivashchenko A.T.,Research Institute of Biology and Biotechnology Problems
Russian Journal of Plant Physiology | Year: 2014

The sites of miR396 binding to mRNA of genes encoding growth-regulating transcription factors (GRF) were identified for Arabidopsis thaliana, Glycine max, Hordeum vulgare, Medicago truncatula, Oryza sativa, Sorghum bicolor, Solanum lycopersicum, Triticum aestivum, Vitis vinifera, and Zea mays. The free energy of interaction of miR396 with mRNA was calculated, and the schemes of nucleotide linking in the binding sites were determined. In mRNA of GRF genes of all studied plants, miR396-binding sites were located in the protein-coding part and encoded oligopeptides RSRKPVE or RSRKHVE. The regulation of the expression of plant GRF genes by miR396 family is discussed. © Pleiades Publishing, Ltd., 2014.

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