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Kostenko K.V.,Research Institute of Applied Acoustics | Kryukov Y.S.,Research Institute of Applied Acoustics
Acoustical Physics | Year: 2016

A technique for detecting direct signal pulses based on steep rising edges of acoustic pressure is developed. The technique consists in calculating the mirror derivative of the received signal and normalizing it in a specific manner. This makes it possible to amplify weak direct signals and suppress strong reflected ones. A key feature of this technique is that it ensures a high probability of detection of direct signal pulses while keeping the number of false detections at a minimum. © 2016, Pleiades Publishing, Ltd.


Krylsky D.V.,Research Institute of Applied Acoustics | Gushchin A.P.,Research Institute of Applied Acoustics | Gushchin S.A.,Research Institute of Applied Acoustics | Dezhurov S.V.,Research Institute of Applied Acoustics | And 3 more authors.
Journal of Analytical Chemistry | Year: 2016

A device is developed for automated immunochromatographic assay, designed for one-time tests at the place of occurrence of a disease. The device comprises a turntable platform for up to five strips placed simultaneously, a unit for sample collection and microdosing to a test strip, a detection unit, and a microprocessor control system. A manual, semiautomatic, or automatic operation mode may be selected. Colloidal quantum dots with a luminescence peak from the visible to near-infrared spectral region (600–800 nm) are used as fluorescent labels. A UV-LED or a laser at 365–480 nm can be used as an excitation system; the detection device is a spectrometer with an operation range of 500–800 nm, based on a CCD series of TCD1304DG sensors (Toshiba). RFID tags of samples can re read and the analysis results can be sent to an external computer. The device is made in a plastic case for carrying. © 2016, Pleiades Publishing, Ltd.


Bagratashvili V.N.,Russian Academy of Sciences | Vakshtein M.S.,Research Institute of Applied Acoustics | Zavorotnyi Y.S.,Moscow State University | Krotova L.I.,Russian Academy of Sciences | And 5 more authors.
Inorganic Materials: Applied Research | Year: 2010

A method of diffusion introduction of CdSe quantum dots (QDs) into polymer microparticles in supercritical carbon dioxide was proposed, implemented, and studied. Photoluminescence (PL) spectra of fine nanocomposite powders based on polymethyl methacrylate/polystyrene and CdSe QDs were measured and analyzed. Produced samples are characterized by a pronounced PL band with a general maximum at 620 nm. It was found that QD introduction into polymer microparticles by impregnation in a supercritical carbon dioxide medium efficiently stabilizes them in space and significantly increases photochemical stability of their luminescence characteristics. © Pleiades Publishing, Ltd., 2010.


PubMed | Research Institute of Applied Acoustics, Russian Academy of Sciences and Moscow State University
Type: | Journal: Journal of virological methods | Year: 2016

Three lateral flow assays (LFAs) for the detection of antibodies against glycoprotein B (gB) of Aujeszkys disease virus (ADV) in swine sera: a competitive double antibody sandwich LFA without a preincubation step (CDAS-gB-LFA), a CDAS-gB-LFA with a preincubation step (pCDAS-gB-LFA), and a competitive direct gB-LFA have been developed and were compared with each other and with a gB-ELISA. The assays are based on monoclonal antibodies to immunodominant epitopes of ADV gB. The pCDAS-gB-LFA proved to be the most specific and sensitive assay to detect antibodies directed to ADV gB. The specificity and sensitivity of the pCDAS-gB-LFA with the use of an LFA reader for test line intensity measurements were 97.6 and 94.9%, respectively. The lower diagnostic sensitivity of the pCDAS-gB-LFA compared to a gB-ELISA reflects its reduced analytical sensitivity, which was shown in titration experiments with positive sera. The pCDAS-gB-LFA, using the reader-based and visual detection modes, showed good agreement in respect to specificity; however, the LFA reader detection provided a higher diagnostic and analytical sensitivity compared to visual detection. The developed pCDAS-gB-LFA is a rapid, sensitive, and specific method for the detection of antibodies to ADV gB and can be used for screening ADV-infected swine in unvaccinated herds.


Ivanov O.N.,Research Institute of Applied Acoustics
Pattern Recognition and Image Analysis | Year: 2014

A general method of adaptation of pixel-by-pixel background subtraction models designed for a fixed camera is developed in the case of a PTZ camera mounted on a mobile platform. The method involves the use of two identical pixellevel background models. The first one is directly applied to the classification of the current scene, while the second one is used to prepare the first model for the transformation to the new coordinate system, the one closer to the current position of the PTZ video camera. The chosen solution makes it possible to eliminate a large number of false positives, which inevitably occur after each transformation of the background model. The experimental verification of the developed method using two well-known background models GMM and ViBe demonstrated good quality of the scene classification and low computational load. © 2014 Pleiades Publishing, Ltd.

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