Entity

Time filter

Source Type


Sakabe I.,Research Institute National Center for Geriatrics and Gerontology | Asai A.,Research Institute National Center for Geriatrics and Gerontology | Iijima J.,Research Institute National Center for Geriatrics and Gerontology | Maruyama M.,Research Institute National Center for Geriatrics and Gerontology
Immunity and Ageing | Year: 2012

Background: We recently isolated and identified Zizimin2 as a functional factor that is highly expressed in murine splenic germinal center B cells after immunization with T-cell-dependent antigen. Zizimin2 was revealed to be a new family member of Dock (dedicator of cytokinesis), Dock11, which is the guanine nucleotide exchange factor for Cdc42, a low-molecular-weight GTPase. However, the molecular function of Zizimin2 in acquired immunity has not been elucidated.Results: In this study, we show that the protein expression of Zizimin2, which is also restricted to lymphoid tissues and lymphocytes, is reduced in aged mice. Over-expression of full-length Zizimin2 induced filopodial formation in 293T cells, whereas expression of CZH2 domain inhibited it. Stimulation of Fcγ receptor and Toll-like receptor 4 triggered Zizimin2 up-regulation and Cdc42 activation in bone marrow-derived dendritic cells.Conclusions: These data suggest that Zizimin2 is an immune-related and age-regulated guanine nucleotide exchange factor, which facilitates filopodial formation through activation of Cdc42, which results in activation of cell migration. © 2012 Sakabe et al; licensee BioMed Central Ltd. Source

Discover hidden collaborations