Research Institute for Veterinary Science

Seoul, South Korea

Research Institute for Veterinary Science

Seoul, South Korea
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Yi S.S.,Research Institute for Veterinary Science | Hwang I.K.,Research Institute for Veterinary Science | Shin J.H.,Research Institute for Veterinary Science | Baek S.H.,Seoul National University | And 2 more authors.
BMB Reports | Year: 2010

Homozygous staggerer (RORasg/sg) mice showed a severe ataxia caused by cerebellum degeneration. Decreased and dysfunctional Rora is a main cause of this neurologic phenotype. The phenotype of staggerer mice has been well known in cerebellum. However, there has been rarely reported about cerebrum even though of staggerer is expressed in merely cerebellum but hippocampus, thalamus, cortex, and olfactory bulb. The expressions of Ki67, doublecortin (DCX), and NeuN, which are cell proliferation, neuronal differentiation and mature neuron markers, respectively, were measured with immunohistechemistry in dentate gyrus in staggerer mice in order to uncover whether staggerer can affect the change in dentate gyrus. The immunoreactivities of DCX and NeuN were significantly reduced in the dentate gyrus of staggerer mice than normal control, while Ki67 were rarely unchanged in staggerer mice. These results suggest that staggerer mutation has an influence on the neuronal differentiation and development not only in cerebellum but also in dentate gyrus.


Park S.J.,Research Institute for Veterinary Science | Shin S.,Seoul National University | Koo O.J.,Research Institute for Veterinary Science | Koo O.J.,Seoul National University | And 5 more authors.
Experimental and Molecular Medicine | Year: 2012

Transplantation of islet cells into diabetic patients is a promising therapy, provided that the islet cells are able to evade host immune rejection. With improved islet viability, this strategy may effectively reverse diabetes. We applied 2% calcium alginate to generate small and large capsules to encapsulate porcine neonatal pancreatic cell clusters (NPCCs) using an air-driven encapsulator. After encapsulation, the viability was assessed at 1, 4, 7, 14 and 28 days and secretion of functional insulin in response to glucose stimulation were tested at days 14 and 28. Selective permeability of the small alginate capsules was confirmed using various sizes of isothiocyanate-labeled dextran (FITC-dextran). Encapsulation of NPCCs was performed without islet protrusion in the small and large capsules. The viability of NPCCs in all experimental groups was greater than 90% at day 1 and then gradually decreased after day 7. The NPCCs encapsulated in large capsules showed significantly lower viability (79.50 ± 2.88%) than that of naïve NPCCs and NPCCs in small capsule (86.83 ± 2.32%, 87.67 ± 2.07%, respectively) at day 7. The viability of naïve NPCCs decreased rapidly at day 14 (75.67 ± 1.75%), whereas the NPCCs encapsulated in small capsules maintained (82.0 ± 2.19%). After 14 and 28 days NPCCs' function in small capsules (2.67 ± 0.09 and 2.13 ± 0.09) was conserved better compared to that of naïve NPCCs (2.04 ± 0.25 and 1.53 ± 0.32, respectively) and NPCCs in large capsules (2.04 ± 0.34 and 1.13 ± 0.10, respectively), as assessed by a stimulation index. The small capsules also demonstrated selective permeability. With this encapsulation technique, small capsules improved the viability and insulin secretion of NPCCs without islet protrusion.


Lee S.-J.,Research Institute for Veterinary Science | Lee J.,University of California at San Diego | Song S.,Chonnam National University | Lim K.-T.,Chonnam National University
Food and Chemical Toxicology | Year: 2016

This study was carried out to investigate the anti-inflammatory potentials of a 38 kDa glycoprotein isolated from Styrax japonica Siebold et al Zuccarini (SJSZ glycoprotein). We found that SJSZ glycoprotein has concentration-dependent scavenging activity against DPPH and hydroxyl radicals in the cell-free systems. In colonic epithelial cells (HCT116 cells), the results showed that SJSZ glycoprotein inhibits the production of reactive oxygen species (ROS) induced by glucose/glucose oxidase (G/GO) in a concentration-dependent manner. Experimental mouse colitis was induced by adding dextran sulfate sodium (DSS) to the drinking water at a concentration of 4% (w/v) for 7 days. We figured out that administration of SJSZ glycoprotein (10 mg/kg) lowers the levels of disease activity index, myeloperoxidase activity, and histological inflammation in DSS-treated mice. In addition, SJSZ glycoprotein inhibited plasmic thiobarbituric acid reactive substances (TBARS) formation, nitric oxide (NO) production, and lactate dehydrogenase (LDH) release, accompanying the inhibition of colonic inflammatory signal proteins (NF-κB, iNOS, and COX-2) and inflammation-related cytokines (IL-1β, IL-6, and TNF-α). These results indicate that SJSZ glycoprotein inhibits oxidative and pro-inflammatory responses in mouse colitis. © 2015 Elsevier Ltd.


Lee C.H.,Institute of Neurodegeneration and Neuroregeneration | Moon S.M.,Sacred Heart College | Yoo K.-Y.,Institute of Neurodegeneration and Neuroregeneration | Choi J.H.,Institute of Neurodegeneration and Neuroregeneration | And 8 more authors.
Brain Research | Year: 2010

Delayed neuronal death following transient cerebral ischemia is mixed with apoptosis and necrosis, and the activation of microglia are activated after the ischemic insult. In the present study, we examined the long-term changes in neuronal degeneration and microglial activation in the gerbil hippocampal CA1 region after 5 min of transient cerebral ischemia using specific markers for neuronal damage and microliosis. Transient ischemia-induced neuronal death was shown in CA1 pyramidal cells 4 days after ischemia/reperfusion (I/R). However, neuronal degeneration of the pyramidal cells were observed up to 45 days in the CA1 region after I/R. Microglial activation was also observed in the CA1 region after I/R. Isolectin B4- (IB4) immunoreactive (+) microglia appeared in the CA1 region 4 days after I/R. On the other hand, ionized calcium-binding adapter molecule 1 (Iba-1)+ microglia was markedly increased after I/R, and peaked at 15 days after I/R. Thereafter, Iba-1 immunoreactivity was decreased with time-dependant manner in the ischemic CA1 region. These results indicate that neuronal degeneration of CA1 pyramidal cells may last about 45 days in the CA1 region after ischemic damage, and microglial activation may be diverse according to their function, such as phagocytosis, after I/R. © 2010 Elsevier B.V. All rights reserved.


Cho S.Y.,Research Institute for Veterinary Science | Cho S.Y.,Seoul National University | Cho S.Y.,Mammalian Genetics Unit | Chai J.C.,Hanyang University | And 4 more authors.
Molecules and Cells | Year: 2013

Epigenetic modifications affect gene expression and thereby govern a wide range of biological processes such as differentiation, development and tumorigenesis. Recent initiatives to define genome-wide DNA methylation and histone modification profiles by microarray and sequencing methods have led to the construction of databases. These databases are repositories for international epigenetic consortiums or provide mining results from PubMed, but do not integrate the epigenetic information with gene expression changes. In order to overcome this limitation, we constructed EPITRANS, a novel database that visualizes the relationships between gene expression and epigenetic modifications. EPITRANS uses combined analysis of epigenetic modification and gene expression to search for cell function-related epigenetic and transcriptomic alterations (Freely available on the web at http://epitrans.org). © The Korean Society for Molecular and Cellular Biology. All rights reserved.


Poyarkov Jr. N.A.,Moscow State University | Che J.,CAS Kunming Institute of Zoology | Min M.-S.,Research Institute for Veterinary Science | Kuro-O M.,Hirosaki University | And 5 more authors.
Zootaxa | Year: 2012

We describe four new species of Asian Clawed salamanders of the genus Onychodactylus (Caudata: Hynobiidae), based on fresh material collected during fieldwork in Japan, Korea, the Russian Far East and northeastern China between 2003 and 2010, as well as older voucher specimens deposited in several museums. Our analyses comprise all species currently recognized within this genus across its entire distribution range. We follow an integrative taxonomic approach by combining detailed morphological comparative analyses with molecular phylogenetic analyses. We find significant differences among species in this genus, based on morphological and molecular data, which resulted in the recognition and description of four new species within this genus. The new species have uncorrected molecular divergences of over 4.5-7.4% and 1.9-4.1% to their closest relatives in the mitochondrial COI and 16S rRNA genes respectively. In the molecular analyses, we found two very divergent lineages in Korea and Japan that need further investigation, as detailed morphological data are not available for them. We also discuss our approach to delimit species on salamanders. For the new species described in this group we evaluate their threat status according to IUCN criteria: O. koreanus sp. nov. Min, Poyarkov & Vieites and O. nipponoborealis sp. nov. Kuro-o, Poyarkov & Vieites are classified as Least Concern, while O. zhaoermii sp. nov. Che, Poyarkov & Yan and O. zhangyapingi sp. nov. Che, Poyarkov, Li & Yan are classified as Vulnerable (Vu2a). © 2012 Magnolia Press.


Lee S.-J.,Research Institute for Veterinary Science | Jung Y.H.,Research Institute for Veterinary Science | Ryu J.M.,Research Institute for Veterinary Science | Jang K.K.,Seoul National University | And 2 more authors.
International Journal of Medical Microbiology | Year: 2016

The disruption of gastrointestinal tight junctions and their colonization evoked by enteric pathogens are hallmarks of the pathogenesis. Vibrio (V.) vulnificus, VvpE, is an elastase which is responsible for host surface adherence and vascular permeability; however, the functional roles of VvpE in the pathogenesis of V. vulnificus (WT) are poorly understood. In the present study, we have investigated the role of VvpE in regulation of intestinal tight junctions and the colonization of WT. We found that mutation of the vvpE gene from V. vulnificus (vvpE mutant) prevents intestinal tight/adherens junction dysregulation due to a WT infection and maintains the physiological level of the epithelial paracellular permeability. Interestingly, the vvpE mutant exhibited defective intestinal colonization abilities, whereas WT colonization was significantly elevated in the ileum in a time-dependent manner. Finally, the vvpE mutant negated the enterotoxicity, the breakdown of red blood cells, and pro-inflammatory responses, all of which are induced by the WT infection. In addition, the results of a LC-MS/MS analysis showed that VvpE contributes to WT pathogenesis in multiple ways by interacting with intestinal proteins, including β-globin, Annexin A2, Annexin A4, F-actin, and intelectin-1b. These results demonstrate that VvpE plays important role in promoting the tight junction disruption and intestinal colonization of V. vulnificus and that it also has the ability to interact with the intestinal proteins responsible for microbial pathogenesis. © 2015 Elsevier GmbH.


Park O.K.,Institute of Neurodegeneration and Neuroregeneration | Yoo K.-Y.,Institute of Neurodegeneration and Neuroregeneration | Lee C.H.,Institute of Neurodegeneration and Neuroregeneration | Choi J.H.,Institute of Neurodegeneration and Neuroregeneration | And 5 more authors.
Journal of the Neurological Sciences | Year: 2010

Melatonin is synthesized from serotonin by the action of arylalkylamine N-acetyltransferase (AANAT) and hydroxyindole-O-methyltransferase. In this study, we observed cellular changes of arylalkylamine N-acetyltransferase (EC 2.3.1.87; AANAT) in the hippocampal CA1 region at various time points after ischemia/reperfusion. In vehicle-treated sham group, AANAT immunoreaction was detected in pyramidal neurons of the CA1 region. AANAT immunoreactivity in the neurons was highest 2 days and disappeared from 4 days after ischemia/reperfusion. From 3 days after ischemia/reperfusion, AANAT immunoreaction was expressed in astrocytes in the strata oriens and radiatum of the CA1 region. AANAT protein and mRNA levels were significantly increased 2 days after ischemia/reperfusion, and markedly decreased from 5 days after ischemia/reperfusion. The repeated administration of melatonin (10 mg/kg, i.p.) 3 times (once a day) to gerbils before ischemia/reperfusion significantly reduced ischemia-induced hyperactivity as well as neuronal death compared to those in the vehicle-treated ischemia group. Melatonin treatment also maintained AANAT immunoreactivity and its protein levels in the CA1 region after ischemia/reperfusion. These results suggest that the reduction of AANAT in ischemic CA1 region is associated with delayed neuronal death following transient ischemia, and melatonin treatment shows neuroprotection with maintenance of AANAT levels in the ischemic CA1 region. © 2010 Elsevier B.V. All rights reserved.


Gomez D.K.,Research Institute for Veterinary Science | Mori K.-i.,Japan National Research Institute of Fisheries And Environment of Inland Sea | Okinaka Y.,Hiroshima University | Nakai T.,Hiroshima University | Park S.C.,Research Institute for Veterinary Science
Aquaculture | Year: 2010

A total of 360 samples of trash fish/mollusk used for feeding cultured marine fish were collected from 4 stations of National Research Institute of Aquaculture, Fisheries Research Agency, Japan. The brain or eyes of the samples were examined by reverse transcription-polymerase chain reaction (RT-PCR) and nested PCR assays to detect the coat protein gene of betanodavirus. Two species of trash fish/mollusk samples: Japanese jack mackerel Trachrus japonicus and Japanese common squid Todarodes pacificus were positive for betanodavirus and the sequences of the nested PCR products (177 nucleotides) of these two species were closely related (98%) to the redspotted grouper nervous necrosis virus (RGNNV) genotype. Sevenband grouper Epinephelus septemfasciatus were intramuscularly injected (106 TCID50/fish) with betanodavirus isolates from trash fish/mollusk samples. The fish displayed abnormalities typical of viral nervous necrosis (VNN) infection, and mortalities ranged from 90 to 100%. These results suggest that trash fish/mollusk can be a source of betanodaviruses for cultured fish and that they pose a serious risk for outbreaks of VNN in susceptible cultured fish. © 2010 Elsevier B.V. All rights reserved.


PubMed | Catholic Kwandong University, A-Life Medical, University of Houston, Korea University and 5 more.
Type: | Journal: Nature communications | Year: 2016

Dopaminergic (DA) neurons are involved in the integration of neuronal and hormonal signals to regulate food consumption and energy balance. Forkhead transcriptional factor O1 (FoxO1) in the hypothalamus plays a crucial role in mediation of leptin and insulin function. However, the homoeostatic role of FoxO1 in DA system has not been investigated. Here we report that FoxO1 is highly expressed in DA neurons and mice lacking FoxO1 specifically in the DA neurons (FoxO1 KO

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