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Lee S.-J.,Research Institute for Veterinary Science | Lee J.,University of California at San Diego | Song S.,Chonnam National University | Lim K.-T.,Chonnam National University
Food and Chemical Toxicology | Year: 2016

This study was carried out to investigate the anti-inflammatory potentials of a 38 kDa glycoprotein isolated from Styrax japonica Siebold et al Zuccarini (SJSZ glycoprotein). We found that SJSZ glycoprotein has concentration-dependent scavenging activity against DPPH and hydroxyl radicals in the cell-free systems. In colonic epithelial cells (HCT116 cells), the results showed that SJSZ glycoprotein inhibits the production of reactive oxygen species (ROS) induced by glucose/glucose oxidase (G/GO) in a concentration-dependent manner. Experimental mouse colitis was induced by adding dextran sulfate sodium (DSS) to the drinking water at a concentration of 4% (w/v) for 7 days. We figured out that administration of SJSZ glycoprotein (10 mg/kg) lowers the levels of disease activity index, myeloperoxidase activity, and histological inflammation in DSS-treated mice. In addition, SJSZ glycoprotein inhibited plasmic thiobarbituric acid reactive substances (TBARS) formation, nitric oxide (NO) production, and lactate dehydrogenase (LDH) release, accompanying the inhibition of colonic inflammatory signal proteins (NF-κB, iNOS, and COX-2) and inflammation-related cytokines (IL-1β, IL-6, and TNF-α). These results indicate that SJSZ glycoprotein inhibits oxidative and pro-inflammatory responses in mouse colitis. © 2015 Elsevier Ltd.

Cho S.Y.,Research Institute for Veterinary Science | Cho S.Y.,Seoul National University | Cho S.Y.,Mammalian Genetics Unit | Chai J.C.,Hanyang University | And 4 more authors.
Molecules and Cells | Year: 2013

Epigenetic modifications affect gene expression and thereby govern a wide range of biological processes such as differentiation, development and tumorigenesis. Recent initiatives to define genome-wide DNA methylation and histone modification profiles by microarray and sequencing methods have led to the construction of databases. These databases are repositories for international epigenetic consortiums or provide mining results from PubMed, but do not integrate the epigenetic information with gene expression changes. In order to overcome this limitation, we constructed EPITRANS, a novel database that visualizes the relationships between gene expression and epigenetic modifications. EPITRANS uses combined analysis of epigenetic modification and gene expression to search for cell function-related epigenetic and transcriptomic alterations (Freely available on the web at http://epitrans.org). © The Korean Society for Molecular and Cellular Biology. All rights reserved.

Poyarkov Jr. N.A.,Moscow State University | Che J.,CAS Kunming Institute of Zoology | Min M.-S.,Research Institute for Veterinary Science | Kuro-O M.,Hirosaki University | And 5 more authors.
Zootaxa | Year: 2012

We describe four new species of Asian Clawed salamanders of the genus Onychodactylus (Caudata: Hynobiidae), based on fresh material collected during fieldwork in Japan, Korea, the Russian Far East and northeastern China between 2003 and 2010, as well as older voucher specimens deposited in several museums. Our analyses comprise all species currently recognized within this genus across its entire distribution range. We follow an integrative taxonomic approach by combining detailed morphological comparative analyses with molecular phylogenetic analyses. We find significant differences among species in this genus, based on morphological and molecular data, which resulted in the recognition and description of four new species within this genus. The new species have uncorrected molecular divergences of over 4.5-7.4% and 1.9-4.1% to their closest relatives in the mitochondrial COI and 16S rRNA genes respectively. In the molecular analyses, we found two very divergent lineages in Korea and Japan that need further investigation, as detailed morphological data are not available for them. We also discuss our approach to delimit species on salamanders. For the new species described in this group we evaluate their threat status according to IUCN criteria: O. koreanus sp. nov. Min, Poyarkov & Vieites and O. nipponoborealis sp. nov. Kuro-o, Poyarkov & Vieites are classified as Least Concern, while O. zhaoermii sp. nov. Che, Poyarkov & Yan and O. zhangyapingi sp. nov. Che, Poyarkov, Li & Yan are classified as Vulnerable (Vu2a). © 2012 Magnolia Press.

Yi S.S.,Research Institute for Veterinary Science | Hwang I.K.,Research Institute for Veterinary Science | Shin J.H.,Research Institute for Veterinary Science | Baek S.H.,Seoul National University | And 2 more authors.
BMB Reports | Year: 2010

Homozygous staggerer (RORasg/sg) mice showed a severe ataxia caused by cerebellum degeneration. Decreased and dysfunctional Rora is a main cause of this neurologic phenotype. The phenotype of staggerer mice has been well known in cerebellum. However, there has been rarely reported about cerebrum even though of staggerer is expressed in merely cerebellum but hippocampus, thalamus, cortex, and olfactory bulb. The expressions of Ki67, doublecortin (DCX), and NeuN, which are cell proliferation, neuronal differentiation and mature neuron markers, respectively, were measured with immunohistechemistry in dentate gyrus in staggerer mice in order to uncover whether staggerer can affect the change in dentate gyrus. The immunoreactivities of DCX and NeuN were significantly reduced in the dentate gyrus of staggerer mice than normal control, while Ki67 were rarely unchanged in staggerer mice. These results suggest that staggerer mutation has an influence on the neuronal differentiation and development not only in cerebellum but also in dentate gyrus.

Gomez D.K.,Research Institute for Veterinary Science | Mori K.-i.,Japan National Research Institute of Fisheries And Environment of Inland Sea | Okinaka Y.,Hiroshima University | Nakai T.,Hiroshima University | Park S.C.,Research Institute for Veterinary Science
Aquaculture | Year: 2010

A total of 360 samples of trash fish/mollusk used for feeding cultured marine fish were collected from 4 stations of National Research Institute of Aquaculture, Fisheries Research Agency, Japan. The brain or eyes of the samples were examined by reverse transcription-polymerase chain reaction (RT-PCR) and nested PCR assays to detect the coat protein gene of betanodavirus. Two species of trash fish/mollusk samples: Japanese jack mackerel Trachrus japonicus and Japanese common squid Todarodes pacificus were positive for betanodavirus and the sequences of the nested PCR products (177 nucleotides) of these two species were closely related (98%) to the redspotted grouper nervous necrosis virus (RGNNV) genotype. Sevenband grouper Epinephelus septemfasciatus were intramuscularly injected (106 TCID50/fish) with betanodavirus isolates from trash fish/mollusk samples. The fish displayed abnormalities typical of viral nervous necrosis (VNN) infection, and mortalities ranged from 90 to 100%. These results suggest that trash fish/mollusk can be a source of betanodaviruses for cultured fish and that they pose a serious risk for outbreaks of VNN in susceptible cultured fish. © 2010 Elsevier B.V. All rights reserved.

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