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Back D.A.,Bundeswehr Hospital Berlin | Back D.A.,Charité - Medical University of Berlin | Bormann N.,Charité - Medical University of Berlin | Calafi A.,University of California at Davis | And 8 more authors.
International Orthopaedics | Year: 2016

Purpose: Surgical procedures to prevent osteomyelitis after trauma can be supported by local application of antibiotics. This in-vitro study investigated the release and impact of antibiotics from implant coatings against bacteria associated with combat-related osteomyelitis. Methods: K-wires were coated with poly(D,L-lactide) and ciprofloxacin, gentamicin, colistin, daptomycin or cefoxitin in different concentrations. The release was quantified and antimicrobial activity tested for different gram-positive or gram-negative bacteria, alone and in combination. To exclude toxic effects, primary osteoblast-like cells were exposed to antibiotic coating concentrations. Results: All antibiotics alone and in combination showed an initial burst release with dose dependent antimicrobial activity and no negative effects on osteoblast-like cells, except for cefoxitin. Conclusions: Implant coatings can be customized with single or double antibiotic coatings to effectively fight different bacteria and also mixed infections in the treatment of a combat-acquired osteomyelitis. However, optimal drug load and degradation behaviour of individual antibiotics have to be considered. © 2016, SICOT aisbl.

Zech J.,Research Institute for Special Analyses | Zech J.,TU Berlin | Manowski A.,Research Institute for Special Analyses | Manowski A.,TU Berlin | And 7 more authors.
Deutsche Lebensmittel-Rundschau | Year: 2015

A stable isotope dilution assay (SIDA) was developed to quantify bisphenol A (BPA), bisphenol F (BPF), and bisphenol S (BPS) from thermal paper by high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS). The SIDA was verified for BPA, BPF, and BPS resulting in average recoveries from 106 to 115 %. d4-lsotope standards were applied to determine bisphenols contained on supermarket receipts (n = 14) from Berlin, Germany. BPA values of 0.07 to 20.1 mg/g paper weight were determined. One receipt contained 6.43 mg/g BPS; BPF was not detected. Synthesis of 2, 6-dideuterio-4-[1-(3, 5-dideuterio-4-hydroxy-phenyl)-1-methyl-ethyl]phenol (BPA-d4), 2, 6-dideuterio-4-[(3, 5-dideuterio-4-hydroxy-phenyl)methyl]phenol (BPF-d4), and 2, 6-dideuterio-4-(3, 5-dideuterio-4-hydroxy-phenyl)sulfonyl-phenol (BPS-d4) is described. BPA and BPF were labeled directly via acid induced hydrogen-deuterium exchange reaction in deuterium oxide, while BPS was deuterated by labeling 4-(4-hydroxyphenyl)sulfanylphenol followed by oxidation of the intermediate.

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