Hwang D.-S.,Hanyang University |
Lee J.-S.,Research Institute for Natural science |
Lee K.-W.,Research Institute for Natural science |
Rhee J.-S.,Hanyang University |
And 5 more authors.
Comparative Biochemistry and Physiology - C Toxicology and Pharmacology | Year: 2010
Ecdysteroids are steroid hormones that play an important role in development, growth, molting of larva, and reproduction in the Arthropoda. The effect of ecdysteroids is mediated by its binding to ecdysteroid receptor (EcR). To investigate the role of EcR during development and the effect to environmental stressors on EcR expression in a copepod, we isolated and characterized cDNA and 5′-promoter region of the Tigriopus japonicus EcR (TJ-EcR), and studied mRNA expression pattern. The full-length TJ-EcR cDNA sequence was 1962 bp in length and the open reading frame encoded 546 amino acids. The deduced TJ-EcR protein contained well-conserved DNA-binding domain and ligand-binding domain. Phylogenetic analysis revealed that TJ-EcR was clustered with the EcR of other crustaceans. TJ-EcR mRNA was expressed in a developmental stage-specific manner: high in early developmental stages and low in the adult stage. Significantly elevated expression of the TJ-EcR gene in adults was detected at hypersalinity (42 ppt) and high temperature (35 °C) condition. The 5′-flanking region of TJ-EcR gene contains heat shock protein 70 response elements, implying that the environmental stressors may affect its expression via the stress-sensor. In addition, bisphenol A (100 μg/L) repressed TJ-EcR expression. Our results suggest that TJ-EcR could be a biomarker for the monitoring of the impact of environmental stressors in copepods. © 2010 Elsevier Inc. All rights reserved.
Eum D.-Y.,Research Institute for Natural science |
Byun J.-Y.,Research Institute for Natural science |
Yoon C.-H.,Research Institute for Natural science |
Seo W.-D.,NICS |
And 7 more authors.
Anti-Cancer Drugs | Year: 2011
A combined treatment with conventional chemotherapies can enhance the effectiveness of chemotherapeutic agents against cancers. Here, we have shown that the naturally occurring triterpenoids synergistically enhance the response of cervical cancer cells to taxol. Of the triterpenoid compounds, pristimerin enhanced the anticancer effect of taxol with the highest efficiency by combination. Pristimerin synergizes with taxol to inhibit clonogenic survival and tumor growth in nude mice, and to enhance cell death in cervical cancer cells. A combined treatment with taxol and pristimerin induced cervical cancer cell death by increasing intracellular reactive oxygen species levels, upregulation of death receptor death receptor 5 (DR5), activation of Bax, and dissipation of mitochondrial membrane potential. Treatment with N-acetyl-L-cysteine, a thiol-containing antioxidant completely blocked combined treatment-induced Bax translocation as well as DR5 upregulation. Moreover, inhibition of Jun N-terminal kinase/c-Jun pathway attenuated cell death by blocking DR5 upregulation and Bax activation. These results indicate that the triterpenoid, pristimerin, synergistically enhances taxol response of cervical cancer cells through DR5 expression and Bax activation. Furthermore, the reactive oxygen species-dependent activation of the Jun N-terminal kinase/c-Jun pathway is required for the DR5 upregulation and Bax activation. The molecular mechanism revealed by this study may aid in the design of future combination cancer therapies against cells with intrinsically reduced sensitivity to taxol. © 2011 Wolters Kluwer Health | Lippincott Williams & Wilkins.
Kim M.Y.,Research Institute for Natural science |
Park J.,Hanyang University |
Lee J.J.,Research Institute for Natural science |
Ha D.H.,Research Institute for Natural science |
And 5 more authors.
Nucleic Acids Research | Year: 2014
Requiem (REQ/DPF2) was originally identified as an apoptosis-inducing protein in mouse myeloid cells and belongs to the novel Krüppel-type zinc finger d4-protein family of proteins, which includes neuro-d4 (DPF1) and cer-d4 (DPF3). Interestingly, when a portion of the REQ messenger ribonucleic acid (mRNA) 3′ untranslated region (3′UTR), referred to as G8, was overexpressed in K562 cells, β-globin expression was induced, suggesting that the 3′UTR of REQ mRNA plays a physiological role. Here, we present evidence that the REQ mRNA 3′UTR, along with its trans-acting factor, Staufen1 (STAU1), is able to reduce the level of REQ mRNA via STAU1-mediated mRNA decay (SMD). By screening a complementary deoxyribonucleic acid (cDNA) expression library with an RNA-ligand binding assay, we identified STAU1 as an interactor of the REQ mRNA 3′UTR. Specifically, we provide evidence that STAU1 binds to putative 30-nucleotide stem-loop-structured RNA sequences within the G8 region, which we term the protein binding site core; this binding triggers the degradation of REQ mRNA and thus regulates translation. Furthermore, we demonstrate that siRNA-mediated silencing of either STAU1 or UPF1 increases the abundance of cellular REQ mRNA and, consequently, the REQ protein, indicating that REQ mRNA is a target of SMD. © 2014 The Author(s) 2014.
Lee K.S.,CHA Medical University |
Yu J.,University of Ulsan |
Shim D.,Research Institute for Natural science |
Choi H.,Hanyang University |
And 4 more authors.
PLoS ONE | Year: 2016
Background: Allergic rhinitis (AR) is the most common allergic disease but little is known about the difference of local immune responses in children and adults with AR. Objective: To compare local immune responses between children and adults with AR and nonallergic rhinitis (NAR), and to investigate whether the association of local and systemic immune responses is different between the two age groups. Methods: Fifty-one patients with chronic rhinitis were enrolled and grouped into children (N = 27, mean age 7.2 years) and adults (N = 24, mean age 29.9 years). Diagnosis of AR was based on symptoms, skin prick tests and serum specific IgEs. Nasal lavage (NAL) fluids were collected from all subjects and used to measure the levels of total IgE, specific IgEs to house dust mites (Dp and Df), and cytokines (TNF-α, IL-4, IL-10, IL-17A and IFN-γ). Flow cytometry was used to measure inflammatory cell types in NAL fluids. Results: AR had significantly increased local levels of total IgE and specific IgEs to Dp and Df compared with NAR in both age groups (P < 0.05). Nasal eosinophils % (P = 0.01) was significantly increased only in children with AR. Local-systemic correlations of total IgE (r = 0.662, P = 0.000) and eosinophil % (r = 0.461, P = 0.015) between the peripheral blood and NAL fluids were found only in children. Moreover, children had correlations between total IgE and eosinophil % in the peripheral blood (r = 0.629, P = 0.001) and in NAL fluids (r = 0.373, P = 0.061). Conclusion: Elevated local IgE is a common feature of AR in children and adults. Local measures in NAR showed naïve state of immune response which disagree with the hypothesis of local allergic rhinitis. Children showed intense local inflammation and close local-systemic interactions compared to adults supporting pediatric AR as a distinct feature. © 2016 Lee et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Kang M.-J.,Yale University |
Kang M.-J.,Brown University |
Yoon C.M.,Yale University |
Yoon C.M.,Brown University |
And 9 more authors.
American Journal of Respiratory Cell and Molecular Biology | Year: 2015
Chitinase 3-like 1 (Chi3l1), which is also called YKL-40 in humans and BRP-39 inmice, is the prototypic chitinase-like protein. Recent studies have highlighted its impressive ability to regulate the nature of tissue inflammation and the magnitude of tissue injury and fibroproliferative repair. This can be appreciated in studies that highlight its induction after cigarette smoke exposure, during which it inhibits alveolar destruction and the genesis of pulmonary emphysema. IL-18 is also known to be induced and activated by cigarette smoke, and, in murine models, the IL-18 pathway has been shown to be necessary and sufficient to generate chronic obstructive pulmonary disease-like inflammation, fibrosis, and tissue destruction. However, the relationship between Chi3l1 and IL-18 has not been defined. To address this issue we characterized the expression of Chi3l1/BRP-39 in control and lung-targeted IL-18 transgenic mice. We also characterized the effects of transgenic IL-18 in mice with wild-type and null Chi3l1 loci. The former studies demonstrated that IL-18 is a potent stimulator of Chi3l1/BRP-39 and that this stimulation is mediated via IFN-g-, IL-13-, and IL-17A-dependent mechanisms. The latter studies demonstrated that, in the absence of Chi3l1/BRP-39, IL-18 induced type 2 and type 17 inflammation and fibrotic airway remodeling were significantly ameliorated, whereas type 1 inflammation, emphysematous alveolar destruction, and the expression of cytotoxic T lymphocyte perforin, granzyme, and retinoic acid early transcript 1 expression were enhanced. These studies demonstrate that IL-18 is a potent stimulator of Chi3l1 and that Chi3l1 is an important mediator of IL-18-induced inflammatory, fibrotic, alveolar remodeling, and cytotoxic responses. © Copyright 2015 by the American Thoracic Society.
Hwang K.,Research Institute for Natural science |
Hwang K.,Hanyang University |
Kwak D.,Research Institute for Natural science |
Kwak D.,Hanyang University |
And 8 more authors.
Angewandte Chemie - International Edition | Year: 2011
Plastic pixels: Electrically tunable photonic pixels exhibiting nonvolatile photonic colors are demonstrated by coupling the hysteretic optical properties of PS-b-P2VP block copolymer photonic gels with an electrochemically induced pH gradient. The optical volatility of photonic pixels was tuned by controlling the hysteresis strength and the conversion pH value, which were both highly dependent on the species of anions pairing with pyridinium groups. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Yoo H.J.,Research Institute for Natural science |
Yoon T.H.,Research Institute for Natural science
Journal of Nanoscience and Nanotechnology | Year: 2014
In this study, a simple flow cytometry protocol to evaluate nanoparticle associated biological response was proposed. Particularly, we have evaluated the effect of surface charge on the cellular nanoparticle associations and nanoparticle-induced apoptosis. Significant enhancement in side scattering intensity was observed for the HeLa cells treated with positively charged PLLZnO nanoparticles, suggesting that the PLLZnO nanoparticles may induce cell death via adsorption and endocytosis of the nanoparticles. On the other hand, the negatively charged PAAZnO nanoparticle seems to cause cell death process indirectly via the released Zn ions, with less contribution from cellular association of nanoparticles. Time- and dose-dependent studies on cellular association of ZnO nanoparticles, and ZnO associated reactive oxygen species generation were also performed for the HeLa cells exposed to the PLLZnO nanoparticle. For those cells associated with PLLZnO nanoparticle, a significant enhancement in reactive oxygen species generation was observed even at a lower concentration (10 ppm), which was not observable for the results with the whole cell population. By using this approach, we are able to distinguish biological responses (e.g., reactive oxygen species (ROS) generation) directly related to the cellular associations of NPs from those indirectly related to the cellular associations of NPs, such as the cytotoxicity caused by the NP released metal ions. Copyright © 2014 American Scientific Publishers All rights reserved.
Odor G.,Research Institute for Natural science |
Kelling J.,Helmholtz Center Dresden |
Kelling J.,TU Chemnitz |
Gemming S.,Helmholtz Center Dresden |
Gemming S.,TU Chemnitz
Physical Review E - Statistical, Nonlinear, and Soft Matter Physics | Year: 2014
Extended dynamical simulations have been performed on a (2+1)-dimensional driven dimer lattice-gas model to estimate aging properties. The autocorrelation and the autoresponse functions are determined and the corresponding scaling exponents are tabulated. Since this model can be mapped onto the (2+1)-dimensional Kardar-Parisi-Zhang surface growth model, our results contribute to the understanding of the universality class of that basic system. © 2014 American Physical Society.
Yoon Y.,Hanyang University |
Cho S.,Hanyang University |
Kim S.,Hanyang University |
Choi E.,Hanyang University |
And 4 more authors.
2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2014 | Year: 2014
We report on a microfluidic trap array that separates and captures circulating tumor cells (CTCs) from whole blood. The device is a series array of microfluidic branches that utilizes the difference in flow rates between the bypass channel and the trap channel to allow CTCs in whole blood to be separated and trapped. Once a trap has captured a cell with diameter larger than the narrow trap outlet, additional cells arriving at the branch would flow towards the bypass channel due to its lower flow resistance. Results demonstrated that it was possible to capture CTCs from the whole blood of a mouse with full-blown metastasis. With further developments, the bypass integrated microfluidic trap array could become a useful tool for the early prognosis of cancer metastasis. © 2014 IEEE.
PubMed | Research Institute for Natural science, University of Ulsan, CHA Medical University and Hanyang University
Type: Journal Article | Journal: PloS one | Year: 2016
Allergic rhinitis (AR) is the most common allergic disease but little is known about the difference of local immune responses in children and adults with AR.To compare local immune responses between children and adults with AR and nonallergic rhinitis (NAR), and to investigate whether the association of local and systemic immune responses is different between the two age groups.Fifty-one patients with chronic rhinitis were enrolled and grouped into children (N = 27, mean age 7.2 years) and adults (N = 24, mean age 29.9 years). Diagnosis of AR was based on symptoms, skin prick tests and serum specific IgEs. Nasal lavage (NAL) fluids were collected from all subjects and used to measure the levels of total IgE, specific IgEs to house dust mites (Dp and Df), and cytokines (TNF-, IL-4, IL-10, IL-17A and IFN-). Flow cytometry was used to measure inflammatory cell types in NAL fluids.AR had significantly increased local levels of total IgE and specific IgEs to Dp and Df compared with NAR in both age groups (P < 0.05). Nasal eosinophils % (P = 0.01) was significantly increased only in children with AR. Local-systemic correlations of total IgE (r = 0.662, P = 0.000) and eosinophil % (r = 0.461, P = 0.015) between the peripheral blood and NAL fluids were found only in children. Moreover, children had correlations between total IgE and eosinophil % in the peripheral blood (r = 0.629, P = 0.001) and in NAL fluids (r = 0.373, P = 0.061).Elevated local IgE is a common feature of AR in children and adults. Local measures in NAR showed nave state of immune response which disagree with the hypothesis of local allergic rhinitis. Children showed intense local inflammation and close local-systemic interactions compared to adults supporting pediatric AR as a distinct feature.