Troubsko, Czech Republic
Troubsko, Czech Republic

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Vymyslicky T.,Agricultural Research Ltd | Smarda P.,Masaryk University | Pelikan J.,Research Institute for Fodder Crops Ltd | Cholastova T.,Agricultural Research Ltd | And 5 more authors.
African Journal of Biotechnology | Year: 2012

The Czech national core collection of the red clover (Trifolium pratense L.) was established in 2006 based on previous analyses (2004 to 2005) of morphological and some phytopathological data observed in the field conditions. Input set included 57 tetraploid accessions (varieties and newly bred varieties) and 130 diploid accessions (varieties, newly bred varieties and wild forms collected from nature) of the world collection. The core collection includes 76 accessions. Molecular and phytopathological data on the core collection were obtained after its establishment during 2007. The core collection was from the point of view of resistance to important fungal and viral pathogens evaluated. Tested plants were inoculated by spore-suspension of Fusarium sp. fungi and also by Bean yellow mosaic virus (BYMV). Microsatellites (SSRs) were used for the study of genetic diversity of all the core collections accessions. No clear difference between diploid and tetraploid accessions within the core collection was evidenced by cluster analysis of morphological data. Notable correlation was found only between the seed weight and ploidy level. The relationships among accessions were analysed with hierarchical clustering, separately for the morphological data (50 characters) and the data on SSR polymorphism (11 primers, 23 polymorphic bands). Molecular and morphological data were not significantly correlated. No differences in particular morphological characters were observed to be associated with the presence/absence of particular bands or with the overall pattern of SSR polymorphism. Dendrogram of accessions included into the Czech core collection of the red clover based on morphological data showed separation of items into seven subclusters, while dendrogram based on molecular data showed the separation of items into 13 subclusters. The core collection accessions showed some level of resistance to Fusarium sp. and BYMV infection. The correlation between resistance of core collection accessions to Fusarium sp. and BYMV pathogens was not significant. © 2012 Academic Journals.


Seidenglanz M.,Republic Services | Rotrekl J.,Research Institute for Fodder Crops Ltd | Rotrekl J.,Agricultural Research Ltd | Poslusna J.,Republic Services | And 2 more authors.
Plant Protection Science | Year: 2011

The effects of two pyrethroid (lambda-cyhalothrin, alpha-cypermethrin) and two neonicotinoid (acetamiprid, thiacloprid) insecticides on B. pisorum L. eggs were compared under field conditions in the Czech Republic in 2005-2007. The main objective of the study was to find out what real effects can be expected from the available insecticides registered in Europe when applied at the time of the first egg occurrence on lower pods. In general, the rates of Bruchus pisorum egg (+ first instar larvae) survival were significantly lower with all the insecticides compared in the study, in each of the three years (2005, 2006, 2007). The tested insecticides showed some ovicidal effects and also some larvicidal effects. The tested pyrethroids (lambda-cyhalothrin, alpha-cypermethrin) showed somewhat higher effectiveness in comparison with the neonicotinoids (acetamiprid, thiacloprid). Alpha-cypermethrin was the most effective in all three years. In 2006 and in 2007 this insecticide significantly reduced the rates of egg survival in comparison with acetamiprid. In contrast, acetamiprid was the least effective insecticide in each of the three years.


Soldanova M.,Agricultural Research Ltd | Cholastova T.,Agricultural Research Ltd | Polakova M.,Research Institute for Fodder Crops Ltd | Piakova Z.,Research Institute for Fodder Crops Ltd | Hajkova P.,Research Institute for Fodder Crops Ltd
African Journal of Biotechnology | Year: 2012

Molecular marker technology was used to map quantitative trait loci (QTLs) associated with resistance to Sugarcane mosaic virus (SCMV). This disease is spread worldwide and can cause devastating yield losses in susceptible maize cultivars. The aim of this study was to estimate genetic determination of resistance to SCMV in a maize population consisting of 120 F 3 lines from a cross between maize inbred lines TR56 (susceptible) and TR42 (resistant). This population was evaluated for SCMV resistance in replicated greenhouse trials under artificial inoculation. The method of composite interval mapping (CIM) was employed for QTL analysis. Two genes were mapped on chromosome 6 using simple sequence repeat (SSR) and bulked segregant analyses (BSA), and a gene on chromosome 3 was found to be not significant. The resistance gene Scm1a on chromosome 6 (bin 6.00/6.01) was flanked by microsatellite markers bnlg1043 and phi126 with genetic distances of 1.0 and 0.6 cM, respectively whereas the genetic distances between two flanking markers bnlg1371 and bnlg1600 and the resistance gene Scm1b were 1.0 and 1.1 cM, respectively. Other SSRs from chromosomes 1, 5 and 10 detected no QTL using single marker analysis. © 2012 Academic Journals.


Bucankova A.,Agricultural Research Ltd. | Bucankova A.,Research Institute for Fodder Crops Ltd. | Ptacek V.,Agricultural Research Ltd.
Journal of Apicultural Science | Year: 2012

Several methods for stimulating nest initiation (particularly the use of the Bombus terrestris cocoon) in queen bumblebees of the species B. lapidarius and B. hortorum were compared. For B. lapidarius, it was determined that the percentage success rate for establishing the first egg cell on a cocoon of B. terrestris is similar to that on a conspecific cocoon. Nest establishment, however, was significantly slower on the cocoon of B. terrestris. Moreover, it was determined that queens of B. lapidarius are able to initiate a nest without hibernation. Queens hibernated in the laboratory displayed a similar percentage success rate in establishing an egg cell during stimulation with the cocoon of B. terrestris as did the outdoor queens, but the lab queens established it significantly more slowly. Queens of B. hortorum did not incubate the cocoon of B. terrestris, nor did they establish an egg cell on it.


Loucka R.,Institute of Animal Science | Nedelnik J.,Research Institute for Fodder Crops Ltd. | Lang J.,Research Institute for Fodder Crops Ltd. | Jambor V.,R.Ø.S.A. | And 2 more authors.
Plant, Soil and Environment | Year: 2015

The objective of the experiments was to compare types of maize hybrids harvested at the similar stage of maturity, between one and two thirds of the milk line stage of grain. The study involved 9 hybrids with different parameters that were grown during a period of three subsequent years in two locations with different level of precipitation. Differences in terms of the content of dry matter between the hybrids at different timing of harvests were small (323 ± 22 g/kg), showing no statistical significance (P = 0.34). Comparing the early maturity hybrids with the medium early hybrids (P < 0.05), the former showed a lower content of starch (299 g/kg dry matter (DM)), and the latter showed a slightly higher value (312 g/kg DM) – while the measured proportion of neutral detergent fibre (NDF) among these two hybrids was 453 vs. 428 g/kg DM. Comparing the measured value of the normally ripening hybrids against the stay-green hybrids, the starch was 314 vs. 294 g/kg DM, and NDF was 434 vs. 451 g/kg DM, respectively. As for the comparison between the flint (vitreous) type of grain endosperm vs. dent (floury), measured value of the starch was 298 vs. 323 g/kg DM, and NDF was 451 vs. 427 g/kg DM, respectively. © 2015, Czech Academy of Agricultural Sciences. All rights reserved.


Holubec V.,Czech Republic Crop Research Institute | Vymyslicky T.,Research Institute for Fodder Crops Ltd. | Paprstein F.,Research and Breeding Institute of Pomology Holovousy Ltd.
Czech Journal of Genetics and Plant Breeding | Year: 2010

Conservation of crops is based on ex situ collection into gene banks. Additionally, crop's wild relatives can be conserved in situ, and landraces and obsolete cultivars also can be conserved using the on-farm method. The definition and methodology of on-farm conservation is discussed. On-farm conservation has been set up in the Czech Republic as model examples in several institutions dealing with nature protection, education, cultural conservation, as well as by some private farmers. Problems, plus positive and negative experiences are presented. On-farm conservation in open-air-museums in the natur (skansens) as well as in the national parks, seem to be suitable ways forward for the Czech Republic.


Hloucalova P.,Mendel University in Brno | Skladanka J.,Mendel University in Brno | Horky P.,Mendel University in Brno | Klejdus B.,Mendel University in Brno | And 2 more authors.
Animals | Year: 2016

The aim of the study was to determine phytoestrogen content in fresh-cut legume forage. This issue has been much discussed in recent years in connection with the health and safety of feedstuffs and thus livestock health. The experiments were carried out on two experimental plots at Troubsko and Vatín, Czech Republic during June and July in 2015. Samples were collected of the four forage legume species perennial red clover (variety “Amos”), alfalfa (variety “Holyně”), and annuals Persian clover and Alexandrian clover. Forage was sampled twice at regular three to four day intervals leading up to harvest and a third time on the day of harvest. Fresh and wilted material was analyzed using liquid chromatography-mass spectrometry (LC-MS). Higher levels (p < 0.05) of isoflavones biochanin A (3.697 mg·g-1 of dry weight) and formononetin (4.315 mg·g-1 of dry weight) were found in red clover than in other species. The highest isoflavone content was detected in red clover, reaching 1.001% of dry matter (p < 0.05), representing a risk for occurrence of reproduction problems and inhibited secretion of animal estrogen. The phytoestrogen content was particularly increased in wilted forage. Significant isoflavone reduction was observed over three to four day intervals leading up to harvest. © 2016 by the authors; licensee MDPI, Basel, Switzerland.


Skladanka J.,Mendel University in Brno | Adam V.,Mendel University in Brno | Adam V.,Brno University of Technology | Dolezal P.,Mendel University in Brno | And 6 more authors.
International Journal of Environmental Research and Public Health | Year: 2013

Mycotoxins are secondary metabolites produced by fungal species that have harmful effects on mammals. The aim of this study was to assess the content of mycotoxins in fresh-cut material of selected forage grass species both during and at the end of the growing season. We further assessed mycotoxin content in subsequently produced first-cutting silages with respect to the species used in this study: Lolium perenne (cv. Kentaur), Festulolium pabulare (cv. Felina), Festulolium braunii (cv. Perseus), and mixtures of these species with Festuca rubra (cv. Gondolin) or Poa pratensis (Slezanka). The mycotoxins deoxynivalenol, zearalenone and T-2 toxin were mainly detected in the fresh-cut grass material, while fumonisin and aflatoxin contents were below the detection limits. July and October were the most risky periods for mycotoxins to occur. During the cold temperatures in November and December, the occurrence of mycotoxins in fresh-cut material declined. Although June was a period with low incidence of mycotoxins in green silage, contents of deoxynivalenol and zearalenone in silages from the first cutting exceeded by several times those determined in their biomass collected directly from the field. Moreover, we observed that use of preservatives or inoculants did not prevent mycotoxin production. © 2013 by the authors; licensee MDPI, Basel, Switzerland.


Seidenglanz M.,Republic Services | Rotrekl J.,Research Institute for Fodder Crops Ltd. | Smykalova I.V.A.,Republic Services | Poslusna J.,Republic Services | Kolarik P.,Research Institute for Fodder Crops Ltd.
Plant Protection Science | Year: 2010

Tested seed treatments (two doses of thiamethoxam, thiamethoxam + fludioxonil + metalaxyl-M; two doses of clothianidin + beta-cyfluthrin) showed high effects on pea leaf weevils (Sitona lineatus L.) which approved relatively long-lasting and sufficient protection of several bottom nodes of stipules simultaneously. The effects of foliar treatments (chlorpyrifos + Cypermethrin; acetamiprid, lambda-cyhalothrin) sometimes were evident only on the node which was determined as the youngest node at the time of spraying. The effects of the compared seed and foliar treatments on the reduction of 5. lineauts larvae numbers on roots were not proven as positive. It is possible to conclude that the foliar application had no real effect in this sense at all. However, positive significant effects of thiamethoxam and clothianidin + beta-cyfluthrin on root nodulation in general were recorded. Especially higher doses of the seed treatments increased overall nodulation from 43% till 363%.


Vymyslicky T.,Research Institute for Fodder Crops Ltd.
Czech Journal of Genetics and Plant Breeding | Year: 2010

Altogether, 17 threatened wild plant species of the family Fabaceae, selected in their natural localities, were included in the monitoring. They could have prospects for use in agriculture or horticulture. Most of the monitored species are well conserved within protected areas. Only occasional disturbances can be observed (caused by wild animals, human footpaths, etc.). Declines in species number were observed, especially during dry seasons (2004, 2007). On the other hand, an increase of species numbers was recorded during wet seasons (2006, 2008); this, thanks to the increased occurrence of annual species and ephemerals. For an evaluation of the state of vegetation on each locality, the Shannon diversity index, combined with species richness data, were used. Species growing on extreme (very dry, much insolated, extremely acid, etc.) localities tend to have bigger fluctuations in their population size, but are better protected against human impacts. No negative trends have been observed in the population dynamics of any of the monitored localities.

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