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Troubsko, Czech Republic

Holubec V.,Czech Republic Crop Research Institute | Vymyslicky T.,Research Institute for Fodder Crops Ltd. | Paprstein F.,Research and Breeding Institute of Pomology Holovousy Ltd.
Czech Journal of Genetics and Plant Breeding | Year: 2010

Conservation of crops is based on ex situ collection into gene banks. Additionally, crop's wild relatives can be conserved in situ, and landraces and obsolete cultivars also can be conserved using the on-farm method. The definition and methodology of on-farm conservation is discussed. On-farm conservation has been set up in the Czech Republic as model examples in several institutions dealing with nature protection, education, cultural conservation, as well as by some private farmers. Problems, plus positive and negative experiences are presented. On-farm conservation in open-air-museums in the natur (skansens) as well as in the national parks, seem to be suitable ways forward for the Czech Republic.

Bucankova A.,Agricultural Research Company | Bucankova A.,Research Institute for Fodder Crops Ltd. | Ptacek V.,Agricultural Research Company
Journal of Apicultural Science | Year: 2012

Several methods for stimulating nest initiation (particularly the use of the Bombus terrestris cocoon) in queen bumblebees of the species B. lapidarius and B. hortorum were compared. For B. lapidarius, it was determined that the percentage success rate for establishing the first egg cell on a cocoon of B. terrestris is similar to that on a conspecific cocoon. Nest establishment, however, was significantly slower on the cocoon of B. terrestris. Moreover, it was determined that queens of B. lapidarius are able to initiate a nest without hibernation. Queens hibernated in the laboratory displayed a similar percentage success rate in establishing an egg cell during stimulation with the cocoon of B. terrestris as did the outdoor queens, but the lab queens established it significantly more slowly. Queens of B. hortorum did not incubate the cocoon of B. terrestris, nor did they establish an egg cell on it.

Vymyslicky T.,Research Institute for Fodder Crops Ltd.
Czech Journal of Genetics and Plant Breeding | Year: 2010

Altogether, 17 threatened wild plant species of the family Fabaceae, selected in their natural localities, were included in the monitoring. They could have prospects for use in agriculture or horticulture. Most of the monitored species are well conserved within protected areas. Only occasional disturbances can be observed (caused by wild animals, human footpaths, etc.). Declines in species number were observed, especially during dry seasons (2004, 2007). On the other hand, an increase of species numbers was recorded during wet seasons (2006, 2008); this, thanks to the increased occurrence of annual species and ephemerals. For an evaluation of the state of vegetation on each locality, the Shannon diversity index, combined with species richness data, were used. Species growing on extreme (very dry, much insolated, extremely acid, etc.) localities tend to have bigger fluctuations in their population size, but are better protected against human impacts. No negative trends have been observed in the population dynamics of any of the monitored localities.

Vymyslicky T.,Agricultural Research Company | Smarda P.,Masaryk University | Pelikan J.,Research Institute for Fodder Crops Ltd. | Cholastova T.,Agricultural Research Company | And 5 more authors.
African Journal of Biotechnology | Year: 2012

The Czech national core collection of the red clover (Trifolium pratense L.) was established in 2006 based on previous analyses (2004 to 2005) of morphological and some phytopathological data observed in the field conditions. Input set included 57 tetraploid accessions (varieties and newly bred varieties) and 130 diploid accessions (varieties, newly bred varieties and wild forms collected from nature) of the world collection. The core collection includes 76 accessions. Molecular and phytopathological data on the core collection were obtained after its establishment during 2007. The core collection was from the point of view of resistance to important fungal and viral pathogens evaluated. Tested plants were inoculated by spore-suspension of Fusarium sp. fungi and also by Bean yellow mosaic virus (BYMV). Microsatellites (SSRs) were used for the study of genetic diversity of all the core collections accessions. No clear difference between diploid and tetraploid accessions within the core collection was evidenced by cluster analysis of morphological data. Notable correlation was found only between the seed weight and ploidy level. The relationships among accessions were analysed with hierarchical clustering, separately for the morphological data (50 characters) and the data on SSR polymorphism (11 primers, 23 polymorphic bands). Molecular and morphological data were not significantly correlated. No differences in particular morphological characters were observed to be associated with the presence/absence of particular bands or with the overall pattern of SSR polymorphism. Dendrogram of accessions included into the Czech core collection of the red clover based on morphological data showed separation of items into seven subclusters, while dendrogram based on molecular data showed the separation of items into 13 subclusters. The core collection accessions showed some level of resistance to Fusarium sp. and BYMV infection. The correlation between resistance of core collection accessions to Fusarium sp. and BYMV pathogens was not significant. © 2012 Academic Journals.

Seidenglanz M.,Republic Services | Rotrekl J.,Research Institute for Fodder Crops Ltd. | Smykalova I.V.A.,Republic Services | Poslusna J.,Republic Services | Kolarik P.,Research Institute for Fodder Crops Ltd.
Plant Protection Science | Year: 2010

Tested seed treatments (two doses of thiamethoxam, thiamethoxam + fludioxonil + metalaxyl-M; two doses of clothianidin + beta-cyfluthrin) showed high effects on pea leaf weevils (Sitona lineatus L.) which approved relatively long-lasting and sufficient protection of several bottom nodes of stipules simultaneously. The effects of foliar treatments (chlorpyrifos + Cypermethrin; acetamiprid, lambda-cyhalothrin) sometimes were evident only on the node which was determined as the youngest node at the time of spraying. The effects of the compared seed and foliar treatments on the reduction of 5. lineauts larvae numbers on roots were not proven as positive. It is possible to conclude that the foliar application had no real effect in this sense at all. However, positive significant effects of thiamethoxam and clothianidin + beta-cyfluthrin on root nodulation in general were recorded. Especially higher doses of the seed treatments increased overall nodulation from 43% till 363%.

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