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Gupta S.K.,Central Institute of Fisheries Education | Gupta S.K.,Directorate of Coldwater Fisheries Research | Pal A.K.,Central Institute of Fisheries Education | Sahu N.P.,Central Institute of Fisheries Education | And 3 more authors.
Aquaculture Nutrition | Year: 2015

Six isonitrogenous and isocaloric semi-purified diets were prepared with different levels of microbial levan: control (Basal), T1 (Basal + 2.5 g kg-1 diet), T2 (Basal + 5 g kg-1 diet), T3 (Basal + 7.5 g kg-1 diet), T4 (Basal + 10 g kg-1 diet) and T5 (Basal + 12.5 g kg-1 diet), fed to six groups of fish in triplicate tanks. The results of the 60 days feeding trail showed that supplementation of dietary levan significantly affected the weight gain percentage and specific growth rate of the treatment groups fed at 10 g kg-1 or more levan. Lowest feed conversion ratio (FCR) value and highest survival percentage among levan fed groups were observed with 12.5 g kg-1 incorporation (T5) and was comparable with (T4) group. Significant increase in muscle RNA level and RNA/DNA ratio was observed with the increasing dietary levan. Fish fed 12.5 g kg-1 levan had significantly higher protease, amylase and lipase activities compare with the control group. Lowest Aspartate aminotransferase (AST) activity in the liver and muscle was observed in the T5 group fed with highest level of dietary levan. Overall results conclude that dietary microbial levan incorporation at 12.5 g kg-1 could be used as potent dietary prebiotic for the culture of L. rohita juveniles. © 2015 John Wiley & Sons Ltd.


Havasi M.,Research Institute for Fisheries and Aquaculture NARIC HAKI | Kumar S.,Research Institute for Fisheries and Aquaculture NARIC HAKI | Nagy Z.,Research Institute for Fisheries and Aquaculture NARIC HAKI | Pal L.,University of Pannonia | And 3 more authors.
Israeli Journal of Aquaculture - Bamidgeh | Year: 2015

A feeding trial was conducted for six weeks to determine the effect of the replacement of fishmeal (FM) either with vegetal protein (mainly soybean) and rendered animal protein combined or vegetal protein alone on growth and carcass composition of European catfish, Silurus glanis. One hundred and thirty five fish (average weight 70.90±5.10g ±SD) were randomly distributed into three treatments in triplicate. Fish were fed with either of three isonitrogenous (35% crude protein) and isocaloric (16.70 MJ/kg DE) experimental diets. The diets were: fish meal (FM) based diet, fish meal protein replaced with a combination of vegetal protein and rendered animal protein (AP), and fish meal replaced with vegetal protein only (SM). Weight gain (%), specific growth rate, protein efficiency ratio, and apparent net protein utilization of FM diet fed groups were similar (P>0.05) with AP diet fed group being significantly higher (P < 0.05) than the SM group. Feed conversion ratio with FM diet was similar (P < 0.05) to that of the AP diet, and significantly lower than SM diet. The present experiment indicated that vegetal protein alone cannot replace total FM protein in the diet of Silurus glanis, however, vegetal protein and rendered animal protein combined, can replace total fish meal protein, with no detrimental effect on growth performance, feed utilization, and body composition. © 2015, Israeli Journal of Aquaculture - Bamidgeh. All rights reserved.


Kumar S.,Research Institute for Fisheries and Aquaculture NARIC HAKI | Kumar S.,Central Institute of Fisheries Education | Sahu N.P.,Central Institute of Fisheries Education | Gal D.,Research Institute for Fisheries and Aquaculture NARIC HAKI
Fish and Shellfish Immunology | Year: 2015

The present study was conducted to investigate the strategy to mitigate the immunosuppressive and oxidative stress effect of gelatinized starch in fingerling of Labeo rohita. Fingerlings were either maintained at ambient water temperature (26 °C) or exposed to 32 °C for one week and then subjected to 26 °C for four weeks. Both groups were fed with isoproteinous (30% crude protein) diets containing gelatinized (G) or non-gelatinized (NG) starch. After 5 weeks of feeding trial, fingerlings were challenged by Aeromonas hydrophila and survival rate was recorded for the next 7 days. Serum cortisol and glucose content was significantly (p < 0.05) higher in G starch fed group and decreased with the increase in temperature from 26 to 32 °C, which was consistent for next four week after decrease in temperature from 32 to 26 °C. Lower respiratory burst activity and serum total protein and globulin content in G starch fed group at 26 °C significantly (p < 0.05) increased after elevation of temperature from 26 to 32 °C and levelled off to NG starch fed group. Liver superoxide dismutase (SOD) and catalase (CAT) activity of G starch fed group was significantly higher in group reared at 32 °C compared to 26 °C. After challenge, fish fed G starch showed lower survival rate than that of fish fed NG starch. Subsequently, exposure of elevated temperature (32 °C) for one week significantly increased the survival rate of G starch fed group and levelled off to NG starch fed group. The results of this study indicated that dietary G starch may cause metabolic stress of fingerling L. rohita, as might consequently lead to the decrease antioxidant abilities, depressed immunity and reduced resistance to A. hydrophila infection. Subsequently, exposure of elevated temperature (32 °C) for one week mitigate immunosuppressive and oxidative stress effect of dietary G starch. © 2015 Elsevier Ltd.


Kumari K.,Central Institute of Fisheries Education | Pathakota G.-B.,Central Institute of Fisheries Education | Annam P.-K.,Central Institute of Fisheries Education | Kumar S.,Research Institute for Fisheries and Aquaculture NARIC HAKI | Krishna G.,Central Institute of Fisheries Education
Proceedings of the National Academy of Sciences India Section B - Biological Sciences | Year: 2015

A stable internal control gene or house keeping gene (HKG) is often used to normalise mRNA levels in different samples for expression analysis. In the present study, the authors identified and evaluated three HKGs, beta actin (β-actin), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and elongation factor 1 alpha (EF1α) for gene expression study in Catla (Catla catla). Gene expression levels were quantified by quantitative real-time reverse transcription polymerase chain reaction in different tissues (liver, kidney, intestine, gill, muscle and brain) and developmental stages (0, 3, 6, 12, 24 h, and 5, 7, 9 days post-fertilization). Expression stability was evaluated by comparing the coefficients of variation of the cycle threshold values and stability index. All the tested HKGs exhibited wide expression range. The results showed that β-actin is the most stable gene followed by the EF1α and GAPDH in different tissues whereas GAPDH was most stable gene followed by EF1α and β-actin during embryonic development. © 2015, The National Academy of Sciences, India.


PubMed | Research Institute for Fisheries and Aquaculture NARIC HAKI and Central Institute of Fisheries Education
Type: Journal Article | Journal: Fish & shellfish immunology | Year: 2015

The present study was conducted to investigate the strategy to mitigate the immunosuppressive and oxidative stress effect of gelatinized starch in fingerling of Labeo rohita. Fingerlings were either maintained at ambient water temperature (26 C) or exposed to 32 C for one week and then subjected to 26 C for four weeks. Both groups were fed with isoproteinous (30% crude protein) diets containing gelatinized (G) or non-gelatinized (NG) starch. After 5 weeks of feeding trial, fingerlings were challenged by Aeromonas hydrophila and survival rate was recorded for the next 7 days. Serum cortisol and glucose content was significantly (p < 0.05) higher in G starch fed group and decreased with the increase in temperature from 26 to 32 C, which was consistent for next four week after decrease in temperature from 32 to 26 C. Lower respiratory burst activity and serum total protein and globulin content in G starch fed group at 26 C significantly (p < 0.05) increased after elevation of temperature from 26 to 32 C and levelled off to NG starch fed group. Liver superoxide dismutase (SOD) and catalase (CAT) activity of G starch fed group was significantly higher in group reared at 32 C compared to 26 C. After challenge, fish fed G starch showed lower survival rate than that of fish fed NG starch. Subsequently, exposure of elevated temperature (32 C) for one week significantly increased the survival rate of G starch fed group and levelled off to NG starch fed group. The results of this study indicated that dietary G starch may cause metabolic stress of fingerling L. rohita, as might consequently lead to the decrease antioxidant abilities, depressed immunity and reduced resistance to A. hydrophila infection. Subsequently, exposure of elevated temperature (32 C) for one week mitigate immunosuppressive and oxidative stress effect of dietary G starch.


Gal D.,Research Institute for Fisheries and Aquaculture NARIC HAKI | Pekar F.,Research Institute for Fisheries and Aquaculture NARIC HAKI | Kerepeczki E.,Research Institute for Fisheries and Aquaculture NARIC HAKI
Aquaculture International | Year: 2016

The aim of the study was to survey the nutrient loads of receiving waters and evaluate the nitrogen, phosphorus and organic matter budget of the conventional fishponds in Hungary. The average nitrogen (N), phosphorus (P) and organic matter (OM) concentrations in the filling-up water of the fishponds were 2.51 ± 1.25, 0.57 ± 0.57 and 23.8 ± 13.4 mg l−1, respectively. Contrarily, the N, P and OM concentrations in the effluents of fishponds were 1.64 ± 2.19, 0.37 ± 0.51 and 30.2 ± 20.5 mg l−1, respectively. The sediment nutrient content in the fishponds during the operation was 5.77 ± 4.42 mg g dry sediment−1 for N, 1.25 ± 0.75 for P and 30.5 ± 43.3 for total organic carbon. The retained nutrients represented on average 53 % (84 kg ha−1 year−1) of N, 74 % (21 kg ha−1 year−1) of P and 74 % (2400 kg ha−1 year−1) of OM, introduced into the fishponds. In the fishponds, the ratio of input N, P and OM accumulated in fish biomass was 18.4 ± 6.7, 10.4 ± 4.4 and 6.8 ± 2.1 %, respectively. The investigated fishponds discharged 48 % less N and 62 % less P into the recipient waters than received with the intake water. However, 78 % more OM was discharged with the effluent from the fishponds than received with the inlet and supplement water. © 2016 Springer International Publishing Switzerland

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