Research Institute for Endocrine science

Tehrān, Iran

Research Institute for Endocrine science

Tehrān, Iran
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Tehrani F.R.,Shahid Beheshti University of Medical Sciences | Zarkesh M.,Research Institute for Endocrine science | Tohidi M.,Shahid Beheshti University of Medical Sciences | Azizi F.,Shahid Beheshti University of Medical Sciences | Zadeh-Vakili A.,Research Institute for Endocrine science
Reproduction, Fertility and Development | Year: 2017

Polycystic ovary syndrome (PCOS) is frequently accompanied by insulin resistance (IR). The aim of the present study was to investigate whether the genetic association between insulin resistance and two single nucleotide polymorphisms (SNPs), namely rs7903146 (C/T) in transcription factor 7-like 2 (TCF7L2) and rs1111875 (A/G) in haematopoietically expressed homeobox (HHEX), is affected by PCOS status in Iranian women. The study participants consisted of 582 women with PCOS (cases) referred to the Reproductive Endocrinology Research Center and 504 subjects without PCOS (controls), randomly selected from the Tehran Lipid and Glucose Study. Cases and controls were further subdivided to two groups according to IR status: Those with and without IR. IR was identified on the basis of homeostasis model assessment of insulin resistance (HOMA-IR) ≥2.63. The SNPs in TCF7L2 and HHEX were genotyped by polymerase chain reaction-restriction fragment length polymorphism. There were no significant differences in the distribution of genotypes and alleles between cases and controls (P<0.05). Among cases, the prevalence of the CC, CT and TT genotypes was 37.8%, 46.3% and 15.9%, respectively, whereas the prevalence of the AA, AG and GG genotypes was 13.5%, 46.1% and 40.4%, respectively. In the control group, the prevalence of the CC, CT and TT genotypes was 32.2%, 53.9% and 13.9%, respectively, whereas the prevalence of the AA, AG and GG genotypes was 11.3%, 48.6% and 40.0%, respectively. After adjustment for age and body mass index, the probability of IR was decreased by 49% among carriers of the A allele in the control group (95% confidence interval 0.33-0.78; P≤0.002). The findings of the present study suggest that the association between IR and diabetogenic polymorphisms may be affected by PCOS status. © CSIRO 2017.


Tehrani F.R.,Reproductive Endocrinology Research Center | Noroozzadeh M.,Reproductive Endocrinology Research Center | Zahediasl S.,Research Institute for Endocrine science | Piryaei A.,Shahid Beheshti University of Medical Sciences | Azizi F.,Shahid Beheshti University of Medical Sciences
Experimental Physiology | Year: 2014

New Findings: What is the central question of this study? Would it be possible to produce a rat model of polycystic ovary syndrome (PCOS), in which the fetuses are exposed to testosterone for a short time and exhibit both endocrine and ovarian disturbances similar to PCOS, while maintaining normal reproductive system morphology in adulthood? What is the main finding and its importance? Prenatal exposure to a single dose of testosterone during the critical period of fetal development facilitates the production of a functional rat model of PCOS with minimal morphological disorders in adulthood. Production of a functional rat model that resembles many features of PCOS may contribute to a better understanding of this syndrome. Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders in women, with a prevalence of 8-12% during the reproductive years. In the present study, using prenatal exposure to a single dose of testosterone during the critical period of fetal development, we aimed to introduce an enhanced rat model that would exhibit both endocrine and ovarian disturbances similar to PCOS, while maintaining normal reproductive system morphology in adulthood. Ten pregnant rats were injected s.c. with 5 mg free testosterone on gestational day 20, while control rats received only solvent. The development and function of the reproductive system in female offspring were examined in adulthood. Prenatally androgenized offspring had irregular oestrous cycles compared with control animals, and their anogenital and anovaginal distances were increased compared with control rats (P < 0.001). No significant differences were observed in the lengths of the vagina and clitoris or the number of nipples between the two groups. Levels of testosterone and luteinizing hormone and the luteinizing hormone/follicle-stimulating hormone ratio were increased in prenatally androgenized offspring compared with control animals (P < 0.05). The numbers of preantral and antral follicles in the ovaries of prenatally androgenized offspring were also increased compared with control rats (P = 0.07 and P < 0.01, respectively). The number of corpora lutea was decreased in prenatally androgenized offspring compared with control rats. Cystic follicles were observed in the ovaries of prenatally androgenized offspring. Prenatal exposure to a single dose of testosterone during the critical period of fetal development could facilitate the development a functional rat model of PCOS in adulthood, with minimal morphological disorders in the reproductive system. © 2014 The Physiological Society.


Tehrani F.R.,Shahid Beheshti University of Medical Sciences | Tehrani F.R.,Research Institute for Endocrine science | Solaymani-Dodaran M.,Tehran University of Medical Sciences | Solaymani-Dodaran M.,University of Nottingham | And 3 more authors.
Journal of Clinical Endocrinology and Metabolism | Year: 2013

Context: Anti-Mullerian hormone (AMH) has already been used for prediction of age at menopause with promising results. Objective: We aimed to improve our previous prediction of age at menopause in a population-based cohort by including all eligible subjects and additional follow-up time. Design and Setting: All reproductive-aged women who met our eligibility criteria were selected from the Tehran Lipid and Glucose Study. The serum concentration of AMH was measured at the time of recruitment, and participant's date of menopause was recorded over a 10-year follow-up. Subjects: Atotal of 1015 women, aged 20 to 50 years, with regular and predictable menstrual cycles at the initiation of the study were recruited. Main Outcome Measure: The actual ages at menopause were compared with the predicted ones obtained from accelerated failure time model. Results:Weobserved 277 occurrences of menopause. Median menopausal age was 50 years (range 30.1-58.2 years). The median (SD) of differences between the actual menopausal age and those predicted by our model was 0.5 (2.5) years. Model adequacy (measured by C-statistics) for correct prediction of age at menopause was 92%. The estimated ages at menopause and their 95% confidence intervals for a range of values of AMH and age were calculated and summarized in a table. Conclusions: Using a model built on age and AMH, we can predict age at menopause many years earlier. This could provide opportunities for interventions in those who are at risk of early or late menopause. Copyright © 2013 by The Endocrine Society.


Pooyan H.,Islamic Azad University at Tehran | Ahmad E.,Research Institute for Endocrine science | Azadeh R.,Shahid Beheshti University of Medical Sciences
Asian Pacific Journal of Cancer Prevention | Year: 2015

Background: Glioblastoma is a highly aggressive and malignant brain tumor. Risk factors are largely unknown however, although several biomarkers have been identified which may support development, angiogenesis and invasion of tumor cells. One of these biomarkers is PAI-1. 4G/5G and A-844G are two common polymorphisms in the gene promotor of PAI 1 that may be related to high transcription and expression of this gene. Studies have shown that the prevalence of the 4G and 844G allele is significantly higher in patients with some cancers and genetic disorders. Materials and Methods: We here assessed the association of 4G/5G and A-844G polymorphisms with glioblastoma cancer risk in Iranians in a case-control study. All 71 patients with clinically confirmed and 140 volunteers with no history and symptoms of glioblastoma as control group were screened for 4G/5G and A-844G polymorphisms of PAI-1, using ARMS-PCR. Genotype and allele frequencies of case and control groups were analyzed using the DeFinetti program. Results: Our results showed significant associations between 4G/5G (p=0.01824) and A-844G (p = 0.02012) polymorphisms of the PAI-1 gene with glioblastoma cancer risk in our Iranian population. Conclusions: The results of this study supporting an association of the PAI-1 4G/5G (p=0.01824) and A-844G (p = 0.02012) polymorphisms with increasing glioblastoma cancer risk in Iranian patients.


Koochakpour G.,Research Institute for Endocrine science | Hosseini-Esfahani F.,Research Institute for Endocrine science | Bahadoran Z.,Research Institute for Endocrine science | Mirmiran P.,National Nutrition and Food Technology Institute | Azizi F.,Shahid Beheshti University of Medical Sciences
Iranian Journal of Endocrinology and Metabolism | Year: 2012

Introduction: Adolescence is an important stage in life for establishing dietary habits. There is little evidence on adolescent dietary patterns and related risk factors. The aim of this study was to determine food patterns among Tehranian adolescents and any associations to anthropometric measures and blood pressure. Materials and Methods: In this study, 507 adolescents, aged 10 to 19 years, were assessed. Dietary intakes were evaluated by means of a validated semi-quantitative questionnaire, and anthropometric indices (weight, height, and waist circumference) and blood pressure were measured and recorded. The Kriska physical activity questionnaire was used for determination of physical activity levels. Results: Using principal component analysis with varimax rotation for 21 food groups, two dietary patterns were derived. In the healthy dietary pattern, whole grain, low fat dairy and vegetables, and in the unhealthy pattern, sweetened drinks, fast food and refined cereal pattern were included. Significant differences were observed in the healthy dietary pattern between boys and girls, as well as, among different age groups. There was significant association between the unhealthy dietary pattern scores and physical activity (P<0.05). After adjustment of confounding factors, significant associations were found between unhealthy dietary pattern scores and body mass index and waist circumference (P<0.05). However, no significant association was observed between blood pressure and the two dietary patterns. Conclusion: Adherence to unhealthy dietary patterns was an important determinant in increase in body mass index and consequently in central obesity among adolescents.


Ghasemi A.,Research Institute for Endocrine science | Zahediasl S.,Research Institute for Endocrine science | Hosseini-Esfahani F.,Nutrition and Endocrine Research Center | Azizi F.,Shahid Beheshti University of Medical Sciences
Annals of Human Biology | Year: 2014

Background: Zinc, a co-factor of antioxidant enzymes, may be involved in the pathophysiology of metabolic syndrome (MetS). Aim: To determine the association between serum zinc concentration and MetS in a population-based study. Subjects and methods: In the Tehran Lipid and Glucose Study, serum zinc concentration was measured by flame atomic absorption spectrometry in 2626 adult participants. After removal of outliers, usable data was available for 2401 participants. Dietary zinc was assessed by a validated semi-quantitative food frequency questionnaire. Results: Compared to those without the MetS, serum zinc concentration was significantly higher in men with syndrome (17.1±4.9 vs 16.5±4.6μmol/L, p=0.035); however, values were comparable in females (16.1±4.8 vs 15.8±4.4, p=0.460). Men in tertile 3 of serum zinc had significantly higher chances of having MetS, even after multivariate adjustment (OR=1.48, 95% confidence interval (CI)=1.07-2.06, p=0.020). Women in tertile 2 of serum zinc had significantly lower chances of having MetS, which remained significant after multivariate adjustment (OR=0.68, 95% CI=0.47-0.99, p=0.046). Conclusion: This study observed a gender difference between serum zinc and MetS, with higher levels being positively associated with MetS in men and having a protective effect in women. Further studies are needed to elucidate the role of zinc status in the development of MetS. © 2014 Informa UK Ltd. All rights reserved.


PubMed | Research Institute for Endocrine science and Shahid Beheshti University of Medical Sciences
Type: Journal Article | Journal: Hypertension research : official journal of the Japanese Society of Hypertension | Year: 2016

Greater adherence to the Mediterranean diet has beneficial effects on the prevention of chronic diseases. In the current study, we investigated the association between the Mediterranean diet score (MDS) and the 6-year incidence of chronic kidney disease (CKD), conducted in the framework of the Tehran Lipid and Glucose Study with 1212 adults, aged 30-71 years. Dietary intake was assessed using a valid and reliable food-frequency questionnaire, and all subjects received scores between 0 and 8 points based on the traditional MDS. The components of the MDS were fruits and nuts, vegetables, legumes, cereals, fish, meat, dairy products and the monounsaturated to saturated fatty acid ratio. The odds ratio (OR) for the occurrence of CKD according to the quartiles of the MDS was assessed by multivariable logistic regression. The mean (s.d.) age of participants (51% male) at baseline was 43.5 (9.4) years. The median (25-75 interquartile range) of MDS for all subjects was 4 (3-5).The incidence of CKD was 19%. After adjustment for all potential confounding variables, individuals in the highest quartile of the MDS were 51% less likely to have CKD than those in the lowest quartile (OR=0.49; 95% confidence interval (CI): 0.30-0.82). Additionally, after further adjustment for baseline estimated glomerular filtration rate (GFR), the inverse association between the MDS and the 6-year incidence of CKD remained significant (OR=0.53; 95% CI: 0.31-0.91). Our findings demonstrate a significant inverse association between the MDS and the risk of incident CKD, indicating that adherence to the Mediterranean diet has favorable effects on the prevention of kidney dysfunction.


PubMed | Research Institute for Endocrine science, National Nutrition and Food Technology Research Institute, Research Center for Gastroenterology and Liver Disease and Shahid Beheshti University of Medical Sciences
Type: Journal Article | Journal: Endocrinology and metabolism (Seoul, Korea) | Year: 2015

Helicobacter pylori infection and subsequent gastric inflammation have been proposed as risk factors for the development of insulin resistance and cardiovascular disease. In this study we assessed the possible association of H. pylori bacterial load, and serum biomarker of gastric inflammation with cardiometabolic risk factors in diabetic patients.In this cross-sectional study, 84 H. pylori-infected type 2 diabetic patients were assessed for anthropometrics, biochemical and clinical measurements. Pearson correlation test, linear, and logarithmic regression curve estimation models were used to assess the association of H. pylori stool antigen (HpSAg) levels, and pepsinogen I (PGI) to pepsinogen II (PGII) ratio with fasting serum glucose, insulin, serum lipid and lipoprotein parameters, malondialdehyde, high-sensitive C-reactive protein (hs-CRP), systolic and diastolic blood pressure, body weight, waist circumference and lipid accumulation product (LAP) index.The mean age of participants was 5410 years, and 44% were men. Mean HpSAg levels and PGI/PGII ratio were 0.240.23 g/mL and 9.99.0, respectively. Higher HpSAg as well as lower PGI/PGII was correlated with higher anthropometric measures and LAP. A significant negative correlation between PGI/PGII ratio and blood pressure (r=-0.21 and r=-0.22, systolic and diastolic, respectively, P<0.05), serum insulin (r=-0.17, P=0.05), and hs-CRP (r=-0.17, P=0.05) was observed. A significant linear association between PGI/PGII ratio with serum triglycerides (=-0.24, P<0.05), serum high density lipoprotein cholesterol (HDL-C; =0.43, P<0.01), and triglycerides/HDL-C ratio (=-0.28, P<0.05) were observed.Higher H. pylori bacterial load and lower PGI/PGII ratio was associated with higher levels of cardiometabolic risk factors in H. pylori infected type 2 diabetic patients.


PubMed | tehran, Tehran, Research Institute for Endocrine science, Shahid Beheshti University of Medical Sciences and University of Tehran
Type: | Journal: Thyroid : official journal of the American Thyroid Association | Year: 2017

Considering the limited data available on the natural course of euthyroidism, this study was designed to evaluate the progression in time from euthyroidism to subclinical or overt hypo - or hyperthyroidism.This study was conducted within the framework of the Tehran Thyroid cohort study (TTS), in which 5783 individuals aged 40.4 0.2 years were followed for 6 years. The overall loss to follow-up rate was 8.3%. After applying exclusion criteria, data of 4204 euthyroid subjects remained for analysis of a six-year natural course analysis. Thyroid function tests (TFT), clinical, and metabolic characteristics were assessed at baseline and every 3 years.The annual incidence rates (95% CIs) of subclinical and overt hypothyroidism were 7.62 (7.39-7.85) and 2.0 (1.94-2.06) per 1000 persons, respectively. For thyroid hyperfunction, the annual incidence rates of subclinical and overt hyperthyroidism were 0.92 (0.90-0.95) and 0.68 (0.66-0.70) per 1000 persons, respectively. Euthyroid persistency was 93.24% during 6 years. Predictive factors for conversion to thyroid dysfunctions were TSH, FT4 and TPOAb levels, gender, and smoking. Criteria for early diagnosis of hypothyroidism, i.e. sensitivity of 94% and specificity of 82% (p < 0.0001) were obtained based on baseline and three-year follow-ups of TFTs and TPOAb. Early diagnosis of hypothyroidism was significantly attributed to impaired glucose tolerance (relative risk with 95% CIs 3.03, 1.36-6.75; p = 0.007), high cholesterol (relative risk with 95% CIs 2.46, 1.45-4.18; p = 0.001), obesity (relative risk with 95% CIs 2.92, 1.64-5.2; p < 0.001), and hypertension (Relative risk with 95% CIs 1.68, 1.53-1.84; p <0.04).This study showed that after a six-year follow-up in an iodine sufficient area, 6.7% of euthyroid subjects were found to progress to thyroid dysfunctions, subclinical hypothyroidism in particular.


Ghasemi A.,Research Institute for Endocrine science | Zahediasl S.,Research Institute for Endocrine science | Azizi F.,Research Institute for Endocrine science
Clinical Biochemistry | Year: 2010

Objectives: To determine reference values for serum nitric oxide metabolites (nitrite + nitrate = NOx) concentrations in adult subjects. Design and methods: Serum NOx concentration was measured, using the Griess method, in 694 non-smoking apparently healthy subjects, randomly selected from a population-based study. The International Federation of Clinical Chemistry guidelines and the robust method were used for determining reference values. Results: The 95% reference values for serum NOx concentration and serum NOx/creatinine ratio were 11.5 to 76.4 μmol/L and 0.111 to 0.729 in men and 10.1 to 65.6 μmol/L and 0.121 to 0.777 in women, respectively. With increasing body mass index, upper limits of serum NOx and the NOx/creatinine ratio increased in women and decreased in men. Serum NOx levels above upper limits predicted both diabetes and metabolic syndrome in women. Conclusions: This study reports the first set of reference values for serum NOx concentration and NOx/creatinine ratio in a relatively large healthy non-smoking population. © 2009 The Canadian Society of Clinical Chemists.

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