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Sultankulova K.T.,Research Institute for Biological Safety Problems RIBSP | Chervyakova O.V.,Research Institute for Biological Safety Problems RIBSP | Kozhabergenov N.S.,Research Institute for Biological Safety Problems RIBSP | Shorayeva K.A.,Research Institute for Biological Safety Problems RIBSP | And 5 more authors.
Scientific World Journal | Year: 2014

The paper describes comparative evaluation of IAVchip DNA microarray, reverse transcription PCR (RT-PCR), and real-time RT-PCR versus virus isolation in chicken embryos and shows their diagnostic effectiveness in detection and subtyping of influenza A virus. The tests were evaluated with use of 185 specimens from humans, animals, and birds. IAVchip DNA microarray demonstrates higher diagnostic effectiveness (99.45%) in early influenza A diagnosis as compared to the real-time PCR (98.38%) and RT-PCR (96.22%), thus showing its clear superiority. Diagnostic sensitivity of IAVchip DNA microarray (100%) exceeds the same of RT-PCR (95.95%) and real-time RT-PCR (97.96%) in the range of estimated confidence intervals. IAVchip DNA microarray and real-time RT-PCR displayed equal diagnostic specificity (98.85%), while diagnostic specificity of RT-PCR was 96.40%. IAVchip DNA microarray has an advantage over the other tests for influenza A diagnosis and virus identification as a more rapid method that allows performing simultaneous detection and subtyping of about tens of specimens within one experiment during 8-10 hours. The developed IAVchip DNA microarray is a general test tool that enables identifying simultaneously 16 hemagglutinin (HA) and 9 neuraminidase (NA) subtypes of influenza A virus and also to screen the influenza A viruses from humans, animals, and birds by M and NP genes. © 2014 K. T. Sultankulova et al.


Tabynov K.,Research Institute for Biological Safety Problems RIBSP | Kydyrbayev Z.,Research Institute for Biological Safety Problems RIBSP | Ryskeldinova S.,Research Institute for Biological Safety Problems RIBSP | Assanzhanova N.,Research Institute for Biological Safety Problems RIBSP | Sansyzbay A.,Research Institute for Biological Safety Problems RIBSP
Vaccine | Year: 2014

We previously created a live vaccine against equine influenza based the new reassortant cold-adapted (Ca) strain A/HK/Otar/6:2/2010. The live vaccine contains surface proteins (HA, NA) from the wild-type virus A/equine/Otar/764/2007 (n{cyrillic}3N8; American Lineage Florida Clade 2), and internal proteins (PB2, PB1, PA, NP, M, NS) from the attenuated Ca donor virus A/Hong Kong/1/68/162/35CA (H3N2). To determine the safety and duration of the protective immune responses, 90 yearlings were intranasally vaccinated in single mode, double mode at an interval of 42 days (107.0 EID50/animal for both vaccinations), or with PBS (control group). Ten animals from each group were challenged with the homologous wild-type virus A/equine/Otar/764/07 (n{cyrillic}3N8) at 1, 2, 3, 4, 5, 6, 9 and 12 months after vaccination. Similarly, 10 animals from each group were challenged with the heterologous wild-type virus A/equine/Sydney/2888-8/07 (n{cyrillic}3N8; American Lineage Florida Clade 1) 12 months after vaccination. The vaccine was completely safe, and single intranasal vaccination of yearlings was capable of inducing statistically significant (from P=0.03 to P<0.0001) clinical and virological protection against the homologous virus; however, only double mode vaccination generated significant (from P=0.02 to P<0.0001) protection against the heterologous virus at 12 months (observation period). Interestingly, this vaccine enables the differentiation of infected and vaccinated animals. On this basis of this study, we recommend double intranasal administration of this vaccine at an interval of 42 days in veterinary practice. © 2014 The Authors.


Koshemetov Z.,Research Institute for Biological Safety Problems RIBSP | Sandybayev N.,Research Institute for Biological Safety Problems RIBSP | Sansyzbay A.,Research Institute for Biological Safety Problems RIBSP | Orynbayev M.,Research Institute for Biological Safety Problems RIBSP | And 8 more authors.
Life Science Journal | Year: 2014

The article contains the biological characterization of pasteurellosis strains isolated from dead saiga antelopes in Kazakhstan. Results demonstrated that pasteurellosis strains isolated from saiga antelopes from different periods had identical biological characteristics.


Kondybaeva Z.,Research Institute for Biological Safety Problems RIBSP | Yershebulov Z.,Research Institute for Biological Safety Problems RIBSP | Sultankulova K.,Research Institute for Biological Safety Problems RIBSP | Nakhanov A.,Research Institute for Biological Safety Problems RIBSP | And 8 more authors.
Life Science Journal | Year: 2014

This paper presents the results of the development of the optimal conditions for new TB-FLU Esat6 2A Ag85A recombinant strain cultivation in Vero cell culture. The studies revealed that the maximum accumulation of the virus in cell culture can be achieved with 0,001 TCD50/cell infecting dose, 34,0 ± 0,5 °C incubation temperature and 72 hours of incubation time. The highest reproductive activity of the virus was obtained using trypsin (Sigma # T4049, Lot10D169, # Lot020M7022T1426-1g) at a concentration 2 μg/ml. The TB-FLU Esat6 2A Ag85A recombinant strain expressing mycobacterial proteins Esat6 and Ag85A with open reading frame of NS1 protein of the influenza virus is genetically stable and capable to maintain enthetic inserts during 10 consecutive passages in Vero cell culture. Subject to the established cultivation parameters the recombinant virus accumulation level reached up to 7,75 ± 0,08 lg TCD50/cm3 with 7,0 log2hemagglutinating titer, which is quite suitable for the development of a TB vaccine for human health care.


Koshemetov Z.,Research Institute for Biological Safety Problems RIBSP | Sansyzbay A.,Research Institute for Biological Safety Problems RIBSP | Sandybayev N.,Research Institute for Biological Safety Problems RIBSP | Abduraimov Y.,Research Institute for Biological Safety Problems RIBSP | And 6 more authors.
Life Science Journal | Year: 2014

The monitoring researches and laboratory confirmation of peste des petits ruminants on the territory of the Central Asia is carried out. As a result of the conducted laboratory researches of the delivered biomaterials from sick, died goats and sheep from various regions of the Republic of Kazakhstan, Tajikistan and Kyrgyzstan it has been demonstrated that virus of this disease is present. The map of spread of peste des petits ruminants on the territory of the Central Asia is made.

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