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Klotchenko S.A.,Research Institute of Influenza | Vasin A.V.,Research Institute of Influenza | Sandybaev N.T.,Research Research Institute for Biological Safety Problems | Plotnikova M.A.,Research Institute of Influenza | And 9 more authors.
Journal of Physics: Conference Series | Year: 2012

Influenza is one of the most widespread respiratory viral diseases, infecting humans, horses, pigs, poultry and some other animal populations. Influenza A viruses (IAV) are classified into subtypes on the basis of the surface hemagglutinin (H1 to H16) and neuraminidase (N1 to N9) glycoproteins. The correct determination of IAV subtype is necessary for clinical and epidemiological studies. In this article we propose an oligonucleotide microarray for subtyping of IAV using universal one-step multisegment RT-PCR fluorescent labeling of viral gene segments. It showed to be an advanced approach for fast detection and identification of IAV. Source

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