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Koh H.S.,Research Institute for Biological Resources | Jo J.E.,Research Institute for Biological Resources | Kweon G.H.,Research Institute for Biological Resources | Ahn N.H.,Research Institute for Biological Resources | And 2 more authors.
Russian Journal of Theriology | Year: 2014

We obtained cytochrome b complete sequences (1140 bp) of the greater horseshoe bat Rhinolophus ferrumequinum from Korea (Jeju Island and mainland Korea), and these sequences were compared to corresponding sequences of R. ferrumequinum, obtained from GenBank, in order to examine genetic divergence among populations within R. ferrumequinum from far-eastern Asia and other parts of the range. Specimens from four populations in far-eastern Asia (Jeju, mainland Korea, northeastern China, and Japan) formed a far-eastern clade and were little differentiated, indicating that our results support a former subspecies classification, recognized R. f. korai and R. f. quelpartis as synonyms of R. f. nippon. In addition, we found that the eastern China clade from Henan is genetically distinct from the far-eastern clade, although individuals from Japan, northeastern China, and eastern China are known as R. f. nippon, and we propose further analyses with additional specimens from China to examine whether or not the eastern China clade is a subpopulation within R. f. nippon. On the other hand, R. ferrumequinum from Eurasia and northern Africa was found to be composed of four clades (far-eastern Asia, eastern China, central China, and western Asia - Europe - northern Africa), and average nucleotide distances between the first and other three clades were 2.15%, 4.10%, and 5.37% respectively. Thus, we found that genetic distances between these clades are correlated with geographic distances between them, and we concluded that further analysis of cytochrome b and other markers from additional specimens of R. ferrumequinum across distributional range is necessary to reexamine its population structure and subspecies classification. © Russian Journal Of Theriology, 2014.

Koh H.S.,Research Institute for Biological Resources | Jo J.E.,Research Institute for Biological Resources | Ahn N.H.,Research Institute for Biological Resources | Lee J.H.,Research Institute for Biological Resources | And 2 more authors.
Journal of Cetacean Research and Management | Year: 2013

Using samples from bycaught finless porpoises, cytochrome b sequences were analysed and phylogenetic trees were constructed. The aims were to: (1) determine genetic divergences within the genus Neophocaena; (2) examine interspecific divergences between N. asiaeorientalis and N. phocaenoides; and (3) examine intraspecific divergence between N.a. asiaeorientalis and N.a. sunamen. For this purpose, complete cytochrome b sequences for 12 N.a. sunameri specimens, collected from fishery markets at Pohang in southeastern Korea, were obtained, and these sequences were compared to the corresponding partial (402bp) and complete (1,140bp) sequences of Neophocaena, obtained from GcnBank. From a maximum likelihood tree with the partial sequences of the two Neophocaena species, two clades were detected, corresponding to the two species, with average genetic distance of 1.64%, four fixed site differences (1.00%), and a Gst value of 0.64, although we did not examine the specimens from Southeast Asia and contiguous South China Sea. Furthermore, from the complete sequences, we recognised a lack of genetic divergence between the two subspecies of N asiaeorientalis, with a Gst value of 0.06 and two pairs of identical sequences between them, indicating that our results do not support current subspecies classification. Thus, we newly found that our cytochrome b sequencing results arc useful for the examination of interspecific and intraspccific divergences in Neohpocaena, although further genetic analyses with additional specimens of Neophocaena across its distributional range are necessary to confirm the findings in this study.

Koh H.S.,Research Institute for Biological Resources | Bayarkhagva D.,National University of Mongolia | Kryukov A.,Russian Academy of Sciences | Zhang M.,Northeast Forestry University | And 3 more authors.
Acta Zoologica Academiae Scientiarum Hungaricae | Year: 2015

Cytochrome b complete sequences (1140 bp) of Sciurus vulgaris from four regions in continental Asia (Korea, northeastern China, far-eastern Russia, and Mongolia) were obtained and analysed with corresponding complete and partial (359 bp) haplotypes of S. vulgaris, obtained from GenBank. These sequences were used to compare our and previous results from partial sequences and to examine population differentiation of S. vulgaris in Eurasia by using complete sequences and by using more numerous specimens. Based on partial sequences from ten Eurasian regions (the four regions in Asia, Japan, Portugal, western China, Russia, and northern and southern Italy), we found that S. vulgaris is composed of two clades (a minor one from southern Italy and a major one from other Eurasian regions). We considered that our results are more conclusive than previous results by other researchers, performed without using haplotypes from Korea, Mongolia, and far-eastern Russia and detected three clades (southern Italy, Russia, and other Eurasia) or two clades with one distinguishable haplotype. Moreover, we found that our results from partial sequences are less informative than those from complete sequences, indicating that cytochrome b complete sequences from Eurasia are needed in order to examine population structure of S. vulgaris. Finally, in this study on population differentiation from cytochrome b complete sequence analyses, we found that genetic divergence of six Eurasian populations, including the four Asian populations, is low, and that our results do not support the current subspecies classification, recognizing 23 subspecies.

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