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Rajsky D.,Technical University In Zvolen | Rajsky M.,Research Institute for Animal Production | Garaj P.,Technical University In Zvolen | Kropil R.,Technical University In Zvolen | And 4 more authors.
European Journal of Wildlife Research | Year: 2016

A study of roe deer fibropapillomatosis, a neoplastic disease with rising occurrence, was conducted in Slovakia during 1998–2014. The first documented case of the disease was identified in 1998, at the district of Senica, in the western part of the country bordering with the Czech Republic. The disease spread from the place of initial occurrence towards the south-eastern regions of Slovakia. During the 17 years of monitoring, the disease spread to 37 districts from the total of 72 districts in the Slovak Republic and 610 cases of roe deer fibropapillomatosis were registered. Examined cases were categorized according to the extent of the lesions as follows: animals with 1 to 10 tumours (53.28 %), 11 to 30 tumours (30.49 %) and more than 31 tumours (16.23 %). The size of tumours was categorized in 45.41 % of the individuals as small (10–50 mm) and in 46.72 % individuals as medium sized (51–100 mm). Large tumours (101 mm and larger) were rare. The predilection site of tumour development in both sexes of roe deer was the skin of the abdomen, followed by forelegs and hind legs, the back and the head. Although the viral aetiology of the disease has been clarified previously, there are still open questions regarding the epidemiology of the disease, particularly about the role of vectors and other environmental factors in its expansion. © 2015, The Author(s).

Mirnawati,Andalas University | Rizal Y.,Andalas University | Marlida Y.,Andalas University | Kompiang I.P.,Research Institute for Animal Production
International Journal of Poultry Science | Year: 2011

An experiment was conducted for the evaluation of Palm Kernel Cake Fermented (PKCF) by Aspergillus niger as a substitute for soybean meal protein in the diet of broiler. The experiment used a Complete Randomize Design (CRD) with 6 treatments and 4 replications. The treatments were arranged as follows: 1) 0% basic ration (0% PKCF), 2) 20% substitution of soybean meal protein with PKCF, 3) 40% substitution of soybean meal protein with PKCF, 4) 60% substitution of soybean meal protein with PKCF, 5) 80% substitution of soybean meal with PKCF, 6) 100% substitution of soybean meal with PKCF. The ration were formulated in iso protein 22% and iso caloric 3000 k cal/kg ration. This study used a completely randomized design with six treatments and four replications. The parameters of this study were feed consumption, body weight gained, feed conversion and percentage of carcass. The result of this study showed that feed consumption, body weight gained, feed conversion and percentage of carcase were not significantly affected (p>0.05) by any treatment. In conclusion the Palm Kernel Cake Fermented (PKCF) by Aspergillus niger can be 100% a substitute of soybean meal protein or 17% in broiler ration. © Asian Network for Scientific Information, 2011.

Bertkova I.,University of P.J. Safarik | Hijova E.,University of P.J. Safarik | Chmelarova A.,University of P.J. Safarik | Mojzisova G.,University of P.J. Safarik | And 4 more authors.
Neoplasma | Year: 2010

Diet interventions and natural bioactive supplements have now been extensively studied to reduce risks of colon cancer, which is one of the major public health problem throughout the world. The objective of our investigation was to study the effects of probiotic, prebiotic, nutritional plant extract, and plant oil on selected biochemical and immunological parameters in rats with colon cancer induced by N,N dimethylhydrazine (DMH). Male and female Wistar albino rats were were fed by a high-fat (HF) diet (10% fat in the diet) and were divided into 9 groups: Control group; PRO group - HF diet supplemented with probiotic Lactobacillus plantarum to provide 3 x 109 c.f.u. of strain/1 ml of medium; PRE group - HF diet supplemented with inulin enriched with oligofructose (2% of HF diet); HES group - HF diet supplemented with plant extract of Aesculus hippocastanum L. (1% of HF diet); OIL group - HF diet comprised Lini oleum virginale (2% of HF diet); and combination of probiotic microorganisms and bioactive compounds in the groups - PRO-PRE, PRO-HES, PRO-OIL, PRE-OIL. Carcinogenesis was iniciated with subcutaneous injection of DMH (20 mg/kg) two times at week interval and dietary treatments were continued for the six weeks. Application of probiotic microorganisms and bioactive compounds in all treated groups significantly decreased the activities of bacterial enzymes (p<0.001), the fecal bile acids concentration (p<0.01; p<0.001) and significantly increased serum TNFα level (p<0.001) in comparison to the control rats. The number of coliforms was reduced in PRO, PRO-PRE, PRO-OIL and PRE-OIL groups and significantly higher count of lactobacilli (p<0.05) was observed in PRO-PRE, PRO-OIL and PRE-OIL groups in compare with the controls. In conclusion, the results of this study indicate that probiotic microorganisms and bioactive compounds could exert a preventive effect on colon carcinogenesis induced by DMH.

Foskolos A.,Autonomous University of Barcelona | Calsamiglia S.,Autonomous University of Barcelona | Chrenkova M.,Research Institute for Animal Production | Weisbjerg M.R.,University of Aarhus | Albanell E.,Autonomous University of Barcelona
Animal | Year: 2015

Kinetics of nutrient degradation in the rumen is an important component of feed evaluation systems for ruminants. The in situ technique is commonly used to obtain such dynamic parameters, but it requires cannulated animals and incubations last several days limiting its application in practice. On the other hand, feed industry relies strongly on NIRS to predict chemical composition of feeds and it has been used to predict nutrient degradability parameters. However, most of these studies were feedstuff specific, predicting degradability parameters of a particular feedstuff or category of feedstuffs, mainly forages or compound feeds and not grains and byproducts. Our objective was to evaluate the potential of NIRS to predict degradability parameters and effective degradation utilizing a wide range of feedstuffs commonly used in ruminant nutrition. A database of 809 feedstuffs was created. Feedstuffs were grouped as forages (FF; n=256), non-forages (NF; n=539) and of animal origin (n=14). In situ degradability data for dry matter (DM; n=665), CP (n=682) and NDF (n=100) were collected. Degradability was described in terms of washable fraction (a), slowly degradable fraction (b) and its rate of degradation (c). All samples were scanned from 1100 to 2500 nm using an NIRSystems 5000 scanning in reflectance mode. Calibrations were developed for all samples (ALL), FF and NF. Equations were validated with an external validation set of 20% of total samples. NIRS equations to predict the effective degradability and fractions a and b of DM, CP and NDF could be evaluated from being adequate for screening (r 2>0.77; ratio of performance to deviation (RPD)=2.0 to 2.9) to suitable for quantitative purposes (r 2>0.84; RPD=3.1 to 4.7), and some predictions were improved by group separation reducing the standard error of prediction. Similarly, the rate of degradation of CP (CP c) and DM (DM c) was predicted for screening purposes (RPDa2 and 2.5 for CP c and DM c, respectively). However, the rate of degradation of NDF was not predicted accurately (NDF c: r 2<0.75; RDP<2). © The Animal Consortium 2015.

Csakyova V.,Constantine the Philosopher University | Szecsenyi-Nagy A.,Hungarian Academy of Sciences | Csosz A.,Hungarian Academy of Sciences | Nagy M.,J. Selye University | And 7 more authors.
PLoS ONE | Year: 2016

The genetic composition of the medieval populations of Central Europe has been poorly investigated to date. In particular, the region of modern-day Slovakia is a blank spot in archaeogenetic research. This paper reports the study of mitochondrial DNA (mtDNA) in ancient samples from the 9th-12th centuries originating from the cemeteries discovered in Nitra-Šindolka and Čakajovce, located in western Slovakia (Central Europe). This geographical region is interesting to study because its medieval multi-ethnic population lived in the so-called contact zone of the territory of the Great Moravian and later Hungarian state formations. We described 16 different mtDNA haplotypes in 19 individuals, which belong to the most widespread European mtDNA haplogroups: H, J, T, U and R0. Using comparative statistical and population genetic analyses, we showed the differentiation of the European gene pool in the medieval period. We also demonstrated the heterogeneous genetic characteristics of the investigated population and its affinity to the populations of modern Europe. © 2016 Csákyová et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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