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Fini M.,Rizzoli Orthopaedic Institute | Fini M.,Research Institute Codivilla Putti | Tschon M.,Rizzoli Orthopaedic Institute | Tschon M.,Research Institute Codivilla Putti | And 9 more authors.
Journal of Bone and Joint Surgery - Series B | Year: 2010

Short intense electrical pulses transiently increase the permeability of the cell membrane, an effect known as electroporation. This can be combined with antiblastic drugs for ablation of tumours of the skin and subcutaneous tissue. The aim of this study was to test the efficacy of electroporation when applied to bone and to understand whether the presence of mineralised trabeculae would affect the capability of the electric field to porate the membrane of bone cells. Different levels of electrical field were applied to the femoral bone of rabbits. The field distribution and modelling were simulated by computer. Specimens of bone from treated and control rabbits were obtained for histology, histomorphometry and biomechanical testing. After seven days, the area of ablation had increased in line with the number of pulses and/or with the amplitude of the electrical field applied. The osteogenic activity in the ablated area had recovered by 30 days. Biomechanical testing showed structural integrity of the bone at both times. Electroporation using the appropriate combination of voltage and pulses induced ablation of bone cells without affecting the recovery of osteogenic activity. It can be an effective treatment in bone and when used in combination with drugs, an option for the treatment of metastases. ©2010 British Editorial Society of Bone and Joint Surgery.

Di Bella C.,Research Institute Codivilla Putti | Di Bella C.,Rizzoli Orthopaedic Institute | Aldini N.N.,Rizzoli Orthopaedic Institute | Lucarelli E.,Research Institute Codivilla Putti | And 7 more authors.
Tissue Engineering - Part A | Year: 2010

Limited incorporation and modest bone remodeling can cause allograft failure. We investigated whether mesenchymal stem cells (MSCs) and osteogenic protein-1 (OP-1) can improve allograft integration. A 3-cm full-size intercalary bone defect was created in the mid-diaphysis of the metatarsal bone of the sheep and it was replaced with an allograft alone (control group), or with MSCs (MSC group), OP-1 (OP-1 group), or MSCs and OP-1 (MSC+OP-1 group). Radiographic results showed a faster and complete integration of the allograft in the MSC+OP-1 group. Histology demonstrated that the amount of new bone was significantly greater inside the graft and a longer vessel penetration in the MSC+OP-1 group than in others. Mechanical strength of the allograft was not compromised by the high rate of bone remodeling. These results demonstrated that the association of MSCs and OP-1 improve bone allograft integration promoting an almost complete bone restoring. © 2010, Mary Ann Liebert, Inc.

Boanini E.,University of Bologna | Torricelli P.,Research Institute Codivilla Putti | Fini M.,Research Institute Codivilla Putti | Bigi A.,University of Bologna
Journal of Materials Science: Materials in Medicine | Year: 2011

Ionic substitution is a powerful tool to improve the biological performance of calcium phosphate based materials. In this work, we investigated the response of primary cultures of rat osteoblasts derived from osteopenic (OOB) bone to strontium substituted hydroxyapatite (SrHA), and to hydroxyapatite (HA) as reference material, compared to normal (N-OB) bone cells. Strontium (Sr) and calcium (Ca) cumulative releases in physiological solution are in agreement with the greater solubility of SrHA than HA, whereas the differences between the two materials are levelled off in DMEM, which significantly reduced ion release. O-OB cells grown on SrHA exhibited higher proliferation and increased values of the differentiation parameters. In particular, Sr substitution increased the levels of proliferation, alkaline phosphatase, and collagen type I, and downregulated the production of interleukin-6 of O-OB cells, demonstrating a promising future of SrHA in the treatment of bone lesions and defects in the presence of osteoporotic bone. © Springer Science+Business Media, LLC 2011.

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