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Garvin R.P.,University of Nebraska Medical Center | Duryee M.J.,Research in Rheumatologic Disease Laboratory | Duryee M.J.,University of Nebraska Medical Center | Klassen L.W.,Research in Rheumatologic Disease Laboratory | And 4 more authors.
Ultrasound in Medicine and Biology | Year: 2012

Cardiovascular disease is a major cause of morbidity and mortality in the world and better prevention and treatment strategies are needed. Studies from this laboratory have shown that perfluorocarbon exposed sonicated dextrose albumin (PESDA) microbubbles bind to inflamed vasculature through interactions with scavenger receptors (SR). This current study details the use of PESDA as a tool for accessing and quantifying the extent of vascular inflammation. Angioplastied rat aortas were evaluated with low mechanical index microbubble imaging techniques contrast pulse sequencing (CPS); Siemens Acuson Sequoia 15L8, 7-15 MHz ultrasound probe with a mechanical index of 0.09 to detect microbubble binding. Real-time polymerase chain reaction (RT-PCR) analysis of angioplastied aortas demonstrated a significantly (p < 0.01) increased expression of both SRs and Interleukin 6 (IL-6). Vessel wall enhancement was quantified using densitometry of CPS ultrasound images and correlated with the upregulated expression of scavenger receptors, Toll-like receptors and IL-6. This study demonstrates that PESDA, in conjunction with CPS ultrasound, is an effective imaging technique to better detect early vascular inflammation and potential cardiovascular disease. © 2012. Source


Anderson D.R.,University of Nebraska Medical Center | Anderson D.R.,82265 Nebraska Medical Center | Poterucha J.T.,82265 Nebraska Medical Center | Mikuls T.R.,Research in Rheumatologic Disease Laboratory | And 9 more authors.
Cytokine | Year: 2013

Biomarkers such as interleukin-6 (IL-6), soluble interleukin-6 receptor (sIL-6R), and high sensitive C-reactive protein (hsCRP) have been reported to be elevated in acute myocardial infarction (AMI). The aim of this study is to determine the relationship between these markers during AMI, as well as their relationship to clinical parameters in an effort to discern their predictive potential in cardiac events.Serum was collected from 73 patients with; AMI, stable coronary artery disease (CAD), and controls during cardiac catheterization. Biomarker levels were determined and correlated with clinical data. IL-6 (11.75. pg/ml, P<. 0.05) and sIL-6R (41,340. pg/ml, P=. 0.05) were elevated in AMI compared with CAD and controls. At presentation, hsCRP was elevated in AMI patients (4.69. mg/L) compared to controls (2.69. mg/L, P<. 0.05); however, there was a significant decrease in hsCRP between AMI (4.69. mg/L) and CAD patients (7.4. mg/L, P<. 0.05). After 24. h post-AMI hsCRP levels were increased compared to stable CAD (60.46. mg/L, P<. 0.05) and were preceded by increased IL-6 at presentation. Soluble Gp130 (sGp130) showed no significant change between AMI, CAD, and control patients. However, sGp130 positively correlated with peak troponin in AMI (. R=. 0.587, P<. 0.01), and negatively correlated with previous AMI (. R=. -0.382, P<. 0.05). Circulating monocyte mRNA expression isolated from selected AMI patients showed an increase in IL-6 mRNA (5.28-fold, P<. 0.01) and a decrease in both IL-6R (0.374-fold, P<. 0.01) and sGp130 mRNA (0.38-fold, P<. 0.01) as compared to CAD and controls.Results demonstrate that IL-6 and sIL-6R are associated with AMI and cardiac injury. These data support the hypothesis that trans-IL-6 receptor binding may alter intracellular signaling, and blocking of IL-6 receptor binding may be pathogenic in AMI. These data may be predictive of mechanism(s) by which plaques become unstable and rupture. © 2013 . Source

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