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Martinez-Diaz R.A.,Autonomous University of Madrid | Ibanez-Escribano A.,Complutense University of Madrid | Burillo J.,Research Center y Tecnologia Agroalimentaria | de las Heras L.,Autonomous University of Madrid | And 4 more authors.
Memorias do Instituto Oswaldo Cruz | Year: 2015

Artemisia absinthium is an aromatic and medicinal plant of ethnopharmacological interest and it has been widely studied. The use of A. absinthium based on the collection of wild populations can result in variable compositions of the extracts and essential oils (EOs). The aim of this paper is the identification of the active components of the vapour pressure (VP) EO from a selected and cultivated A. absinthium Spanish population (T2-11) against two parasitic protozoa with different metabolic pathways: Trypanosoma cruzi and Trichomonas vaginalis. VP showed activity on both parasites at the highest concentrations. The chromatographic fractionation of the VP T2-11 resulted in nine fractions (VLC1-9). The chemical composition of the fractions and the antiparasitic effects of fractions and their main compounds suggest that the activity of the VP is related with the presence of trans-caryophyllene and dihydrochamazulene (main components of fractions VLC1 and VLC2 respectively). Additionally, the cytotoxicity of VP and fractions has been tested on several tumour and no tumour human cell lines. Fractions VLC1 and VLC2 were not cytotoxic against the nontumoural cell line HS5, suggesting selective antiparasitic activity for these two fractions. The VP and fractions inhibited the growth of human tumour cell lines in a dose-dependent manner. © 2015, Fundacao Oswaldo Cruz. All rights reserved.


Alvarez-Rodriguez J.,Research Center y Tecnologia Agroalimentaria | Palacio J.,University of Zaragoza | Tamanini C.,University of Bologna | Sanz A.,Research Center y Tecnologia Agroalimentaria
Journal of Animal Physiology and Animal Nutrition | Year: 2010

The episodic release of luteinizing hormone (LH) and growth hormones (GH) was studied in three suckling regimens and two breeds of Spanish suckled cows. Parda de Montaña (PA) cows (n = 21) were assigned to once-daily, twice-daily or ad libitum (ADLIB) suckling. Pirenaica (PI) cows (n = 7) were used to evaluate the breed effect in twice-daily suckling. Coccygeal blood samples were collected twice weekly during lactation to determine the interval from calving to first ovulation through peripheral progesterone. On day 32 ± 3 post-partum, jugular blood samples were drawn at 15 min intervals during 8 h to analyse circulating LH and GH. The interval to first ovulation was greater in PA cows suckling ADLIB than in restricted suckling treatment (RESTR1), whereas in RESTR2 it did not differ from the other two treatments. There were no differences between PA and PI cows in the interval to first ovulation. RESTR1 cows showed a tendency to have shorter LH peak widths than ADLIB cows. PA cows showed a tendency to have longer LH peak widths than their PI counterparts. There were no differences across treatments or breeds in any of the GH measures of secretion. The LH release was more affected by breed than by suckling frequency, whereas that of GH was not influenced by any of these parameters. The variables that best allowed discrimination between ADLIB and restricted nursing systems were the interval to post-partum first ovulation, LH peak number and the mean GH concentration. © 2009 The Authors. Journal of Animal Physiology and Animal Nutrition © 2009 Blackwell Verlag GmbH.


Oliver A.,Public University of Navarra | Mendizabal J.A.,Public University of Navarra | Ripoll G.,Research Center y Tecnologia Agroalimentaria | Alberti P.,Research Center y Tecnologia Agroalimentaria | Purroy A.,Public University of Navarra
Meat Science | Year: 2010

The SEUROP system is currently in use for carcass classification in Europe. Image analysis and other new technologies are being developed to enhance and supplement this classification system. After slaughtering, 91 carcasses of local Spanish beef breeds were weighed and classified according to the SEUROP system. Two digital photographs (a side and a dorsal view) were taken of the left carcass sides, and a total of 33 morphometric measurements (lengths, perimeters, areas) were made. Commercial butchering of these carcasses took place 24 h postmortem, and the different cuts were grouped according to four commercial meat cut quality categories: extra, first, second, and third. Multiple regression analysis of carcass weight and the SEUROP conformation score (x variables) on meat yield and the four commercial cut quality category yields (y variables) was performed as a measure of the accuracy of the SEUROP system. Stepwise regression analysis of carcass weight and the 33 morphometric image analysis measurements (x variables) and meat yield and yields of the four commercial cut quality categories (y variables) was carried out. Higher accuracy was achieved using image analysis than using only the current SEUROP conformation score. The regression coefficient values were between R2 = 0.66 and R2 = 0.93 (P < 0.001) for the SEUROP system and between R2 = 0.81 and R2 = 0.94 (P < 0.001) for the image analysis method. These results suggest that the image analysis method should be helpful as a means of supplementing and enhancing the SEUROP system for grading beef carcasses. © 2009 Elsevier Ltd. All rights reserved.

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