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Martinez-Diaz R.A.,Autonomous University of Madrid | Ibanez-Escribano A.,Complutense University of Madrid | Burillo J.,Research Center y Tecnologia Agroalimentaria | de las Heras L.,Autonomous University of Madrid | And 4 more authors.
Memorias do Instituto Oswaldo Cruz | Year: 2015

Artemisia absinthium is an aromatic and medicinal plant of ethnopharmacological interest and it has been widely studied. The use of A. absinthium based on the collection of wild populations can result in variable compositions of the extracts and essential oils (EOs). The aim of this paper is the identification of the active components of the vapour pressure (VP) EO from a selected and cultivated A. absinthium Spanish population (T2-11) against two parasitic protozoa with different metabolic pathways: Trypanosoma cruzi and Trichomonas vaginalis. VP showed activity on both parasites at the highest concentrations. The chromatographic fractionation of the VP T2-11 resulted in nine fractions (VLC1-9). The chemical composition of the fractions and the antiparasitic effects of fractions and their main compounds suggest that the activity of the VP is related with the presence of trans-caryophyllene and dihydrochamazulene (main components of fractions VLC1 and VLC2 respectively). Additionally, the cytotoxicity of VP and fractions has been tested on several tumour and no tumour human cell lines. Fractions VLC1 and VLC2 were not cytotoxic against the nontumoural cell line HS5, suggesting selective antiparasitic activity for these two fractions. The VP and fractions inhibited the growth of human tumour cell lines in a dose-dependent manner. © 2015, Fundacao Oswaldo Cruz. All rights reserved.


PubMed | Autonomous University of Madrid, Institute Investigacion Hospital 12 Of Octubre, CSIC - Institute of Agricultural Sciences, Research Center y Tecnologia Agroalimentaria and Complutense University of Madrid
Type: Journal Article | Journal: Memorias do Instituto Oswaldo Cruz | Year: 2015

Artemisia absinthium is an aromatic and medicinal plant of ethnopharmacological interest and it has been widely studied. The use ofA. absinthium based on the collection of wild populations can result in variable compositions of the extracts and essential oils (EOs). The aim of this paper is the identification of the active components of the vapour pressure (VP) EO from a selected and cultivated A. absinthium Spanish population (T2-11) against two parasitic protozoa with different metabolic pathways: Trypanosoma cruzi and Trichomonas vaginalis. VP showed activity on both parasites at the highest concentrations. The chromatographic fractionation of the VP T2-11 resulted in nine fractions (VLC1-9). The chemical composition of the fractions and the antiparasitic effects of fractions and their main compounds suggest that the activity of the VP is related with the presence of trans-caryophyllene and dihydrochamazulene (main components of fractions VLC1 and VLC2 respectively). Additionally, the cytotoxicity of VP and fractions has been tested on several tumour and no tumour human cell lines. Fractions VLC1 and VLC2 were not cytotoxic against the nontumoural cell line HS5, suggesting selective antiparasitic activity for these two fractions. The VP and fractions inhibited the growth of human tumour cell lines in a dose-dependent manner.


Alvarez-Rodriguez J.,Research Center y Tecnologia Agroalimentaria | Palacio J.,University of Zaragoza | Tamanini C.,University of Bologna | Sanz A.,Research Center y Tecnologia Agroalimentaria
Journal of Animal Physiology and Animal Nutrition | Year: 2010

The episodic release of luteinizing hormone (LH) and growth hormones (GH) was studied in three suckling regimens and two breeds of Spanish suckled cows. Parda de Montaña (PA) cows (n = 21) were assigned to once-daily, twice-daily or ad libitum (ADLIB) suckling. Pirenaica (PI) cows (n = 7) were used to evaluate the breed effect in twice-daily suckling. Coccygeal blood samples were collected twice weekly during lactation to determine the interval from calving to first ovulation through peripheral progesterone. On day 32 ± 3 post-partum, jugular blood samples were drawn at 15 min intervals during 8 h to analyse circulating LH and GH. The interval to first ovulation was greater in PA cows suckling ADLIB than in restricted suckling treatment (RESTR1), whereas in RESTR2 it did not differ from the other two treatments. There were no differences between PA and PI cows in the interval to first ovulation. RESTR1 cows showed a tendency to have shorter LH peak widths than ADLIB cows. PA cows showed a tendency to have longer LH peak widths than their PI counterparts. There were no differences across treatments or breeds in any of the GH measures of secretion. The LH release was more affected by breed than by suckling frequency, whereas that of GH was not influenced by any of these parameters. The variables that best allowed discrimination between ADLIB and restricted nursing systems were the interval to post-partum first ovulation, LH peak number and the mean GH concentration. © 2009 The Authors. Journal of Animal Physiology and Animal Nutrition © 2009 Blackwell Verlag GmbH.


PubMed | University of Tours, University of Zaragoza, Research Center y Tecnologia Agroalimentaria and CSIC - Centro de Investigación y Tecnología Agroalimentaria
Type: Journal Article | Journal: The Journal of infectious diseases | Year: 2014

Recently, novel atypical Brucella strains isolated from humans and wild rodents have been reported. They are phenotypically close to Ochrobactrum species but belong to the genus Brucella, based on genetic relatedness, although genetic diversity is higher among the atypical Brucella strains than between the classic species. They were classified within or close to the novel species Brucella inopinata. However, with the exception of Brucella microti, the virulence of these novel strains has not been investigated in experimental models of infection.The type species B. inopinata strain BO1 (isolated from a human) and Brucella species strain 83-210 (isolated from a wild Australian rodent) were investigated. A classic infectious Brucella reference strain, B. suis 1330, was also used. BALB/c, C57BL/6, and CD1 mice models and C57BL/6 mouse bone-marrow-derived macrophages (BMDMs) were used as infection models.Strains BO1 and 83-210 behaved similarly to reference strain 1330 in all mouse infection models: there were similar growth curves in spleens and livers of mice and similar intracellular replication rates in BMDMs. However, unlike strain 1330, strains BO1 and 83-210 showed lethality in the 3 mouse models.The novel atypical Brucella strains of this study behave like classic intracellular Brucella pathogens. In addition, they cause death in murine models of infection, as previously published for B. microti, another recently described environmental and wildlife species.


Cavero J.,CSIC - Aula Dei Experimental Station | Faci J.M.,Research Center y Tecnologia Agroalimentaria | Martinez-Cob A.,CSIC - Aula Dei Experimental Station
Agricultural Water Management | Year: 2016

Nighttime sprinkler irrigation usually results in lower water losses and higher irrigation uniformity compared with daytime sprinkler irrigation due to lower wind speed. However, daytime sprinkler irrigation modifies the microclimatic conditions within the crop canopy which could result in improved crop growth. We studied during three years the effect of daytime and nighttime irrigation on the yield, N content, N uptake, water use efficiency, microclimate and canopy temperature of an alfalfa (Medicago sativa L.) crop irrigated with a solid-set sprinkler system in a semiarid Mediterranean climate. Two irrigation treatments were tested: daytime irrigation and nighttime irrigation. The same irrigation amount was applied in both treatments (552 to 757 mm year−1). The water losses of daytime irrigation (10%) tripled the water losses of nighttime irrigation (3%). In one year, daytime irrigation decreased the mean Christiansen coefficient of uniformity (CU) by 4% and the seasonal CU by 2%. Microclimatic and canopy temperature changes during sprinkler irrigation were higher for daytime irrigation as compared to nighttime irrigation. Daytime irrigation slightly reduced the soil water content of the surface layer (0–0.3 m). The actual seasonal crop evapotranspiration was slightly higher (+3.7%) in the daytime irrigation treatment compared to the nighttime irrigation treatment only in one of the years. The annual alfalfa forage yield (16 to 22 Mg ha−1), N content (3.16 to 3.38%), N uptake (514 to 740 kg ha−1) and water use efficiency (17.7 to 25.9 kg ha−1 mm−1) were not affected by the irrigation time of the day. Although nighttime sprinkler irrigation results in some water saving, daytime sprinkler irrigation of alfalfa can be performed if necessary. © 2016 Elsevier B.V.


Oliver A.,Public University of Navarra | Mendizabal J.A.,Public University of Navarra | Ripoll G.,Research Center y Tecnologia Agroalimentaria | Alberti P.,Research Center y Tecnologia Agroalimentaria | Purroy A.,Public University of Navarra
Meat Science | Year: 2010

The SEUROP system is currently in use for carcass classification in Europe. Image analysis and other new technologies are being developed to enhance and supplement this classification system. After slaughtering, 91 carcasses of local Spanish beef breeds were weighed and classified according to the SEUROP system. Two digital photographs (a side and a dorsal view) were taken of the left carcass sides, and a total of 33 morphometric measurements (lengths, perimeters, areas) were made. Commercial butchering of these carcasses took place 24 h postmortem, and the different cuts were grouped according to four commercial meat cut quality categories: extra, first, second, and third. Multiple regression analysis of carcass weight and the SEUROP conformation score (x variables) on meat yield and the four commercial cut quality category yields (y variables) was performed as a measure of the accuracy of the SEUROP system. Stepwise regression analysis of carcass weight and the 33 morphometric image analysis measurements (x variables) and meat yield and yields of the four commercial cut quality categories (y variables) was carried out. Higher accuracy was achieved using image analysis than using only the current SEUROP conformation score. The regression coefficient values were between R2 = 0.66 and R2 = 0.93 (P < 0.001) for the SEUROP system and between R2 = 0.81 and R2 = 0.94 (P < 0.001) for the image analysis method. These results suggest that the image analysis method should be helpful as a means of supplementing and enhancing the SEUROP system for grading beef carcasses. © 2009 Elsevier Ltd. All rights reserved.

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