Time filter

Source Type

Negrin-Baez D.,University of Las Palmas de Gran Canaria | Navarro A.,University of Las Palmas de Gran Canaria | Lee-Montero I.,Santo Tomás University of Chile | Afonso J.M.,University of Las Palmas de Gran Canaria | And 6 more authors.
Aquaculture Research | Year: 2015

The growth and consolidation of the gilthead seabream (Sparus aurata) industry require improvement based on permanent and cumulative aspects, such as those derived from genetic breeding programmes. Marker Assisted Selection (MAS) by Quantitative Trait Loci (QTL) can be usefully implemented with the appropriate tools. In this study, 138 microsatellite markers from the genetic map of gilthead seabream were redesigned to be amplified under the same PCR conditions. A final set of 13 multiplex PCRs (named ReMsa) with 106 of these markers was developed to cover 100% of the linkage groups. These effective multiplex PCRs enable to optimize QTL searching with a critical reduction in costs and errors. Results showed that the mean value of the number of alleles for 106 markers was 6.9. The mean observed heterozygosity ranged from 0.53 to 0.86, and 74.5% of markers were highly informative according to their polymorphic information content. The correct inheritance and segregation of alleles of each locus was confirmed after genotyping 62 individuals of a full-sib family by these multiplex PCRs. In addition, genetic features of the 20 microsatellite markers that worked correctly but were not included in any multiplex PCR are also reported to provide geneticists with the possibility of including them in more comprehensive screening studies. © 2014 John Wiley & Sons Ltd.

Lee-Montero I.,University of Las Palmas de Gran Canaria | Lee-Montero I.,Santo Tomás University of Chile | Navarro A.,University of Las Palmas de Gran Canaria | Borrell Y.,University of Oviedo | And 12 more authors.
Animal Genetics | Year: 2013

The high number of multiplex PCRs developed for gilthead seabream (Sparus aurata L.) from many different microsatellite markers does not allow comparison among populations. This highlights the need for developing a reproducible panel of markers, which can be used with safety and reliability by all users. In this study, the first standardised panel of two new microsatellite multiplex PCRs was developed for this species. Primers of 138 specific microsatellites from the genetic linkage map were redesigned and evaluated according to their genetic variability, allele size range and genotyping reliability. A protocol to identify and classify genotyping errors or potential errors was proposed to assess the reliability of each marker. Two new multiplex PCRs from the best assessed markers were designed with 11 markers in each, named SMsa1 and SMsa2 (SuperMultiplex Sparus aurata). Three broodstocks (59, 47 and 98 breeders) from different Spanish companies, and a sample of 80 offspring from each one, were analysed to validate the usefulness of these multiplexes in the parental assignation. It was possible to assign each offspring to a single parent pair (100% success) using the exclusion method with SMsa1 and/or SMsa2. In each genotyped a reference sample (Ref-sa) was used, and its DNA is available on request similar to the kits of bin set to genotype by genemapper (v.3.7) software (kit-SMsa1 and kit-SMsa2). This will be a robust and effective tool for pedigree analysis or characterisation of populations and will be proposed as an international panel for this species. © 2013 The Authors, Animal Genetics © 2013 Stichting International Foundation for Animal Genetics.

Garcia-Celdran M.,Technical University of Cartagena | Cutakova Z.,Technical University of Cartagena | Ramis G.,University of Murcia | Estevez A.,IRTA - Institute of Agricultural-Alimentary Research and Technology | And 6 more authors.
Aquaculture | Year: 2016

Lordosis, lack of operculum and failure to inflate the swimbladder constitute a major problem for the gilthead sea bream aquaculture industry. In this study, the effect of the origin of the broodstock on these major anomalies in juvenile sea bream was analyzed. A population of farmed sea bream (n = 909) obtained by industrial mass-spawning from broodstock from three different origins [Cantabrian Sea (CAN), the Atlantic Ocean (ATL) and Mediterranean Sea (MED)] and reared under communal conditions was analyzed from X-ray photograph for skeletal deformities and for uninflated swimbladder. Lordosis and lack of operculum were the most common deformities observed in this study. Juveniles from CAN showed the lowest frequency of skeletal deformities as well as the lowest frequency of uninflated swimbladder. Differences among origins could be partly explained through their different genetic background, but also environmental conditions in the initial facilities and genotype x environment interaction should be considered. A posteriori reconstruction of pedigree was carried out using a microsatellite multiplex (SMsa1) to estimate genetic parameters (heritabilities and genetic correlations) for these traits. Considerable heritabilities were estimated for lordosis [0.53 (0.25–0.77)], lack of operculum [0.37 (0.01–0.81)] and uninflated swimbladder [0.36 (0.12–0.72)] with a positive genetic correlation between uninflated swimbladder and lordosis [0.48 (0.07–0.97)], first reported in this species. All these findings should be relevant for the establishment of successful breeding programs in the aquaculture of this species. Statement of relevance The obtained results provide relevant information to establish a breeding program in gilt head sea bream © 2016 Elsevier B.V.

Garcia-Celdran M.,Technical University of Cartagena | Ramis G.,University of Murcia | Maria-Dolores E.,Servicio de Pesca y Acuicultura Edificio Foro | Penalver J.,Servicio de Pesca y Acuicultura Edificio Foro | And 5 more authors.
Aquaculture International | Year: 2016

The gilthead sea bream (Sparus aurata L.) is one of the most important Sparid farmed in Europe, especially in the Mediterranean area. However, efficient breeding programs for this species are scarce and very little, and it is known concerning their population structure. The present study was mainly designed to genetically characterize, by microsatellite markers, three gilthead sea bream populations sampled along the Spanish coast (Cantabrian Sea, the Atlantic Ocean and Mediterranean Sea) and their progeny with the aim of studying its genetic variability and its genetic structure. Moreover, we evaluated different strategies of broodstocks management (breeders’ number, origin and sex combination) on parental contributions and on effective breeding numbers. That number of breeders in the stock was of great importance to the maximization of contribution since the larger broodstock resulted in larger proportion of parents contributing. Variation in dam and sire spawning incidence and in number of progeny produced per dam and per sire was translated into reduced effective breeding numbers and consequently into expected increased inbreeding rates. Our results highlight the high genetic variability of the studied sea bream populations, as well as the existence of three genetically differentiated populations along the Spanish coast. These findings should be relevant for the establishment of successful breeding programs in aquaculture of the gilthead sea bream. © 2016 Springer International Publishing Switzerland

Gil M.M.,Laboratori dInvestigacions Marines i Aquicultura | Gil M.M.,CSIC - Mediterranean Institute for Advanced Studies | Palmer M.,CSIC - Mediterranean Institute for Advanced Studies | Grau A.,Laboratori dInvestigacions Marines i Aquicultura | And 3 more authors.
Journal of Fish Biology | Year: 2014

The performance of juvenile Argyrosomus regius released off the coast of Mallorca Island (Balearic Islands, Spain) was assessed by comparing the body condition, stable isotope signature and stomach contents of aquaculture-produced A. regius that had been released, fished and returned by fishermen after spending from a few days to >1 year at liberty with A. regius reared under controlled conditions on two contrasting diets (well-fed and unfed). During the first 40 days of the experiment, the condition index (KR) of the returned A. regius and the unfed A. regius followed the same decreasing trend. Thereafter, the KR values of the returned A. regius were significantly higher than those of the unfed A. regius but never reached the values of well-fed A. regius. The δ13C signal of the returned A. regius clearly increased (in comparison with the well-fed A. regius) after they had spent a few months at liberty. The temporal pattern depicted by the stable isotopes and the most likely prey composition inferred from this pattern strongly suggest a shift in diet. The stomach contents of the returned A. regius that had spent <100 days at liberty consisted almost exclusively of decapods. The diet of the few returned A. regius that had spent >100 days at liberty consisted entirely of fishes. Wild A. regius from the remaining fishery on the Spanish coast exhibited the same ontogenetic diet shift from invertebrates to fishes, but at a smaller size threshold. Overall, the results demonstrated that culture-reared A. regius experience adverse conditions during the first days after release into the wild but that at least some A. regius are able to adapt to the natural environment after a few months at liberty. © 2013 The Fisheries Society of the British Isles.

Loading Research Center y Formacion Pesquera y Acuicola El Toruno collaborators
Loading Research Center y Formacion Pesquera y Acuicola El Toruno collaborators