Ojuelos de Jalisco, Mexico
Ojuelos de Jalisco, Mexico

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Canales-Aguirre A.A.,Research Center y Asistencia en Tecnologia | Gomez-Pinedo U.A.,del Hospital Clinico San Carlos | Luquin S.,University of Guadalajara | Ramirez-Herrera M.A.,Laboratorio Of Neurofisiologia | And 2 more authors.
Nutritional Neuroscience | Year: 2012

One of the main concerns regarding organophosphate pesticides (OP) is their possible toxic effects. Doses that do not produce acute toxicity are capable of altering the structure and biochemistry of different tissues and organs by production of reactive oxygen species (ROS). Curcumin (CUR) is the main substance in Curcuma longa (Zingiberacea) rhizome that has strong antioxidant activity. However, the neuroprotective properties of curcumin against oxidative stress induced by prolonged exposure to parathion (PAR) is not clear. Objective: The present work evaluated the protective effect of curcumin against the oxidative damage induced in the rat hippocampus by the OP PAR. Methods: Forty female Wistar rats were distributed in four groups as follows: exposed to PAR by inhalation (PAR group); pre-treated with CUR and then exposed to PAR by inhalation, (CUR + PAR group); exposed to environmental air and treated with CUR in the food (CUR group); and exposed to environmental air (the control group). At the end of the handling process, the concentration of erythrocyte cholinesterase was monitored, as indicator of PAR intoxication and lipoperoxidation, immunohistochemistry for astrocytes, and activated microglia and apoptosis was determined in the hippocampus. Results: In the present study, we show that the administration of CUR (200 mg/kg body weight) significantly diminished the oxidative damage in the hippocampus of rats exposed to the OP PAR. Discussion: These data suggest that CUR may be an alternative to prevent neurodegenerative damage after pesticide exposure. © W.S. Maney & Son Ltd 2012.


Huerta-Saquero A.,National Autonomous University of Mexico | Evangelista-Martinez Z.,Research Center y Asistencia en Tecnologia | Moreno-Enriquez A.,Research Center Cientifica Of Yucatan | Perez-Rueda E.,National Autonomous University of Mexico
Bioengineered | Year: 2013

Bacterial l-asparaginase has been a universal component of therapies for childhood acute lymphoblastic leukemia since the 1970s. Two principal enzymes derived from Escherichia coli and Erwinia chrysanthemi are the only options clinically approved to date. We recently reported a study of recombinant L-asparaginase (AnsA) from Rhizobium etli and described an increasing type of AnsA family members. Sequence analysis revealed four conserved motifs with notable differences with respect to the conserved regions of amino acid sequences of type I and type II L-asparaginases, particularly in comparison with therapeutic enzymes from E. coli and E. chrysanthemi. These differences suggested a distinct immunological specificity. Here, we report an in silico analysis that revealed immunogenic determinants of AnsA. Also, we used an extensive approach to compare the crystal structures of E. coli and E. chrysantemi asparaginases with a computational model of AnsA and identified immunogenic epitopes. A three-dimensional model of AsnA revealed, as expected based on sequence dissimilarities, completely different folding and different immunogenic epitopes. This approach could be very useful in transcending the problem of immunogenicity in two major ways: by chemical modifications of epitopes to reduce drug immunogenicity, and by site-directed mutagenesis of amino acid residues to diminish immunogenicity without reduction of enzymatic activity. © 2013 Landes Bioscience.


Garcia-Marquez E.,Research Center y Asistencia en Tecnologia | Roman-Guerrero A.,Metropolitan Autonomous University | Cruz-Sosa F.,Metropolitan Autonomous University | Lobato-Calleros C.,National Autonomous University of Mexico | And 3 more authors.
Food Hydrocolloids | Year: 2015

Layer-by-layer calcium phosphate (CP)-chitosan (Ch)/mesquite gum (MG) matrixes were designed for protecting and stabilizing red chili oleoresin-in-water emulsions. Potentiometric titration, turbidity and ζ-potential measurements were used for establishing the interaction windows for obtaining CP-Ch complexes. The CP-Ch mass ratio of 1.24 showed the maximum soluble complex formation and a positive charge (~18.5mV) for allow the interaction with MG. Then, red chili oleoresin-in-water emulsions were formed by a layer-by-layer deposition of MG/CP-Ch complexes (C/WRMG/CP-Ch). The results show that high mass ratio of MG was required to form a dense interfacial layer to improve the stability of the system. The droplet size distribution remained unchanged up to 720h. Rheological measurements of C/WRMG/CP-Ch indicate that both, the storage (G') and loss (G″) moduli showed increasing values with frequency (~0.4-11.0Hz) independently of shear strain applied (1, 5, 10%), attributed to the formation of MG/CP-Ch interlinked network in the continuous phase. The emulsion providing best stability and protection against carotenoiddegradation was that made with CP-Ch mass ratio of 1.24 and MG/CP-Ch mass ratio of 10. The mean half-time life of the C/W10 was 502 days compared to 0.24 days for the free unprotected carotenoids. © 2014 Elsevier Ltd.


Barrios-Payan J.A.,Instituto Nacional Of Ciencias Medicas Y Nutricion Salvador Zubiran | Barrios-Payan J.A.,National Autonomous University of Mexico | Castanon-Arreola M.,National Autonomous University of Mexico | Flores-Valdez M.A.,Research Center y Asistencia en Tecnologia | Hernandez-Pando R.,Instituto Nacional Of Ciencias Medicas Y Nutricion Salvador Zubiran
Salud Publica de Mexico | Year: 2010

Mycobacterium tuberculosis, the causal agent of tuberculosis, has affected humankind for approximately 20 000 years. Tuberculosis is a devastating disease, particularly in developing countries. One of its most notable characteristics is latent infection, in which live bacilli persist in the host tissues without clinical manifestations. Thus, the tuberculous bacilli adapt their metabolism to remain viable with low or no replication, avoiding their elimination by the immune system or conventional chemotherapy. Among the several problems that are particularly important to the understanding of this form of tuberculosis, and are not well-known, are the key metabolic steps that allow mycobacteria to remain in a dormant state and its interaction with host immunity. This article reviews some of the most significant biological, clinical and epidemiological aspects of this form of tuberculosis.


Moran-Marroquin G.A.,Research Center y Asistencia en Tecnologia | Cordova J.,University of Guadalajara | Valle-Rodriguez J.O.,Research Center y Asistencia en Tecnologia | Estarron-Espinosa M.,Research Center y Asistencia en Tecnologia | Diaz-Montano D.M.,Research Center y Asistencia en Tecnologia
International Journal of Food Microbiology | Year: 2011

Knowledge of physiological behavior of indigenous tequila yeast used in fermentation process is still limited. Yeasts have significant impact on the productivity fermentation process as well as the sensorial characteristics of the alcoholic beverage. For these reasons a better knowledge of the physiological and metabolic features of these yeasts is required. The effects of dilution rate, nitrogen and phosphorus source addition and micro-aeration on growth, fermentation and synthesis of volatile compounds of two native Saccharomyces cerevisiae strains, cultured in continuous fed with Agave tequilana juice were studied. For S1 and S2 strains, maximal concentrations of biomass, ethanol, consumed sugars, alcohols and esters were obtained at 0.04h -1. Those concentrations quickly decreased as D increased. For S. cerevisiae S1 cultures (at D=0.08h -1) supplemented with ammonium phosphate (AP) from 1 to 4g/L, concentrations of residual sugars decreased from 29.42 to 17.60g/L and ethanol increased from 29.63 to 40.08g/L, respectively. The S1 culture supplemented with AP was then micro-aerated from 0 to 0.02vvm, improving all the kinetics parameters: biomass, ethanol and glycerol concentrations increased from 5.66, 40.08 and 3.11g/L to 8.04, 45.91 and 4.88g/L; residual sugars decreased from 17.67g/L to 4.48g/L; and rates of productions of biomass and ethanol, and consumption of sugars increased from 0.45, 3.21 and 7.33g/L h to 0.64, 3.67 and 8.38g/L h, respectively. Concentrations of volatile compounds were also influenced by the micro-aeration rate. Ester and alcohol concentrations were higher, in none aerated and in aerated cultures respectively. © 2011.


Corona-Gonzalez R.I.,University of Guadalajara | Ramos-Ibarra J.R.,University of Guadalajara | Gutierrez-Gonzalez P.,University of Guadalajara | Pelayo-Ortiz C.,University of Guadalajara | And 2 more authors.
Revista Mexicana de Ingeniera Qumica | Year: 2013

Due to the artisan nature of the production process of tepache, there is no uniformity in fermentation conditions and raw material used. The fermentation is crucial to the final characteristics of tepache and there is a certain degree of ignorance of related organisms and the level of acceptance among consumers. In this study, tepache fermentation conditions (concentration of sugars, initial pH, temperature and fermentation time) were evaluated to enable correlating the formation of products of fermentation (lactic, acetic and ethanol) with the degree of acceptance of the beverage among consumers. Sensory evaluation was measured on a 9-point hedonic scale. Results were analyzed using the response surface methodology (RSM) which showed that the fermentation conditions for higher acceptance were: 22°C, 10% (mass/volume) of sugars (brown sugar), 72 h of fermentation and an initial pH of 5. According to this study, to have a wider acceptance the beverage must contain about 7 g/L of ethanol, no more than 5 g/L of lactic and acetic acid, and 70 g of sucrose/L. Likewise, yeasts present in the fermentation were identified and it was found that Saccharomyces cerevisiae is the predominant species.


Flores-Fernandez J.M.,Research Center y Asistencia en Tecnologia | Padilla-Camberos E.,Research Center y Asistencia en Tecnologia | Castillo Herrera G.A.,Research Center y Asistencia en Tecnologia | Martinez-Velazquez M.,Research Center y Asistencia en Tecnologia
Tropical Biomedicine | Year: 2016

Adulticidal and oviposition- and hatching-altering activities of essential oil extracted from Mexican oregano leaves (Lippia graveolens H.B.K.) (OEO) were evaluated on engorged adult female Rhipicephalus microplus ticks using the adult immersion test bioassay. Twofold dilutions of OEO were tested from a starting dilution of 10% down to 1.25%. Results showed 100% adulticidal activity at 10% OEO concentration and oviposition inhibition of 65.8% and 40.9% at 5.0% and 2.5% OEO concentration, respectively. Egg hatching inhibition was achieved by 26.0% and 11.5% at 5.0% and 2.5% OEO concentration, respectively. These effects could be attributed to OEO major components: thymol, carvacrol and p-cymene, which together account for more than 60.0% of the OEO chemical composition. Mexican oregano could represent a potential source for development of alternative tick control agents. © 2016, Malaysian Society for Parasitology. All rights reserved.


De Lourdes Reyes-Escogido M.,Research Center y Asistencia en Tecnologia | Gonzalez-Mondragon E.G.,Research Center y Asistencia en Tecnologia | Vazquez-Tzompantzi E.,Research Center y Asistencia en Tecnologia
Molecules | Year: 2011

Capsaicin is a unique alkaloid found primarily in the fruit of the Capsicum genus and is what provides its spicy flavor. Generally extracted directly from fruit, high demand has driven the use of established methods to increase production through extraction and characterization. Over time these methods have improved, usually be applying existing techniques in conjunction. An increasingly wide range of potential applications has increased interest in capsaicin. Especially compelling are the promising results of medical studies showing possible beneficial effects in many diseases. Capsaicin's pungency has limited its use in clinical trials to support its biological activity. Characterization and extraction/ synthesis of non-pungent analogues is in progress. A review is made of capsaicin research focusing mainly on its production, synthesis, characterization and pharmacology, including some of its main potential clinical uses in humans. © 2011.


Flores-Payan V.,Research Center y Asistencia en Tecnologia | Herrera-Lopez E.J.,Research Center y Asistencia en Tecnologia | Navarro-Laboulais J.,Universidad Politécnica de Ingeniería | Lopez-Lopez A.,Research Center y Asistencia en Tecnologia
Journal of Industrial and Engineering Chemistry | Year: 2015

A computational model for a reactor used in advanced water treatment was proposed to represent, simulate and predict the mass transfer process of ozone in water (gas-liquid). A graphic interface was designed to simulate the ozone gas transfer into the liquid. In addition, the values of the mass transfer coefficient, and the self-decomposition of ozone, could be determined for different initial conditions. The model for ozone mass transfer was represented by two differential equations derived from the mass balances of the system. Finally a sensitivity analysis was made to determine the effect of the parameters over the operating variables. © 2014 The Korean Society of Industrial and Engineering Chemistry.


Preza A.M.,National Polytechnic Institute of Mexico | Jaramillo M.E.,National Polytechnic Institute of Mexico | Puebla A.M.,Research Center Biomedica Of Occidente | Mateos J.C.,Research Center y Asistencia en Tecnologia | And 2 more authors.
BMC Complementary and Alternative Medicine | Year: 2010

Background: Recently, proteins and peptides have become an added value to foodstuffs due to new knowledge about its structural analyses as related to antioxidant and anticancer activity. Our goal was to evaluate if protein fractions from cacao seeds show antitumor activity on lymphoma murine L5178Y model. The antioxidant activity of these fractions was also evaluated with the aim of finding a correlation with the antitumor activity.Methods: Differential extraction of proteins from unfermented and semi-fermented-dry cacao seeds was performed and characterized by SDS-PAGE and FPLC size-exclusion chromatography. Antitumor activity was evaluated against murine lymphoma L5178Y in BALB/c mice (6 × 104cells i.p.), with a treatment oral dose of 25 mg/kg/day of each protein fraction, over a period of 15 days. Antioxidant activity was evaluated by the ABTS+and ORAC-FL assays.Results: Albumin, globulin and glutelin fractions from both cacao seed type were obtained by differential solubility extraction. Glutelins were the predominant fraction. In the albumin fraction, polypeptides of 42.3 and 8.5 kDa were found in native conditions, presumably in the form of two peptide chains of 21.5 kDa each one. The globulin fraction presented polypeptides of 86 and 57 kDa in unfermented cacao seed that produced the specific-cacao aroma precursors, and after fermentation the polypeptides were of 45 and 39 kDa. The glutelin fraction presented proteins >200 kDa and globulins components <100 KDa in lesser proportion. Regarding the semifermented-dry cacao seed, it was observed that the albumin fraction showed antitumoral activity, since it caused significant decreases (p < 0.05) in the ascetic fluid volume and packed cell volume, inhibiting cell growth in 59.98 ± 13.6% at 60% of the population; while the greatest antioxidant capacity due to free radical scavenging capacity was showed by the albumin and glutelin fraction in both methods assayed.Conclusion: This study is the first report on the biological activity of semifermented-dry cacao protein fractions with their identification, supporting the traditional use of the plant. The albumin fraction showed antitumor and free radical scavenging capacity, however both activities were not correlated. The protein fractions could be considered as source of potential antitumor peptides. © 2010 Preza et al; licensee BioMed Central Ltd.

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