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Cuellar-Cruz M.,Research Center istencia En Tecnologia seno Del Estado Of Jalisco | Vega-Gonzalez A.,University of Guanajuato | Mendoza-Novelo B.,University of Guanajuato | Lopez-Romero E.,University of Guanajuato | And 3 more authors.
European Journal of Clinical Microbiology and Infectious Diseases | Year: 2012

Candida albicans, C. glabrata, C. parapsilosis, and C. tropicalis are able to form biofilms on virtually any biomaterial implanted in a human host. Biofilms are a primary cause of mortality in immunocompromised and hospitalized patients, as they cause recurrent and invasive candidiasis, which is difficult to eradicate. This is due to the fact that the biofilm cells show high resistance to antifungal treatments and the host defense mechanisms, and exhibit an excellent ability to adhere to biomaterials. Elucidation of the mechanisms of antifungal resistance in Candida biofilms is of unquestionable importance; therefore, this review analyzes both the chemical composition of biomaterials used to fabricate the medical devices, as well as the Candida genes and proteins that confer drug resistance. © Springer-Verlag 2012.

Ferreira-Dias S.,University of Lisbon | Sandoval G.,Research Center istencia En Tecnologia seno Del Estado Of Jalisco | Plou F.,CSIC - Institute of Catalysis | Valero F.,Autonomous University of Barcelona
Electronic Journal of Biotechnology | Year: 2013

The potential of lipases (E.C. as biocatalysts for the production of fatty acid derivatives for the food and nutraceutical industries, such as flavouring esters, fatty acid esters of antioxidants and structured lipids, is enormous, mainly due to their high regio- and stereo-selectivities, in addition to the other well-known advantages of enzymatic processes. The replacement of chemical catalysts by lipases presents great benefits in terms of the nutritional properties of the obtained products and environmental care. The reactions performed for the production of these compounds, as well as the best operation conditions, the biocatalysts used, reactor types and operation mode, are addressed in this review. © 2013 by Pontificia Universidad Católica de Valparaíso, Chile.

Espinosa-Andrews H.,Research Center istencia En Tecnologia seno Del Estado Of Jalisco | Enriquez-Ramirez K.E.,Research Center istencia En Tecnologia seno Del Estado Of Jalisco | Garcia-Marquez E.,Metropolitan Autonomous University | Ramirez-Santiago C.,National Autonomous University of Mexico | And 2 more authors.
Carbohydrate Polymers | Year: 2013

The formation of the complex coacervate (CC) phases between gum Arabic (GA) and low molecular weight chitosan (Ch) and the interrelationship between the zeta-potential and viscoelastic properties of the coacervate phase were investigated. The maximum charge difference of biopolymers stock dispersion was displayed in a range of pH between 4.0 and 5.5. Titration experiment between the oppositely charged biopolymers showed that the isoelectric point was found at a biopolymers mass ratio (R[GA:Ch]) of R[5.5:1]. Turbidity, size and ζ-potential of the soluble complexes (SC) showed an interrelation with the complex coacervate yield (CCY). Higher CCY values (82.2-88.1%) were obtained in the range from R[3:1] to R[5.5:1]. Change the R[GA:Ch] in dispersion, make possible to produce CC's phases exhibiting cationic (R[1:1] and R[3:1]), neutral (R [5.5:1]) or anionic (R[9:1] and R[7:1]) charged. All CC's exhibited liquid-viscoelastic behavior at lower frequencies and a crossover between G″ and G′ at higher frequencies. © 2013 Elsevier Ltd.

Rodriguez-Rivera M.P.,Research Center istencia En Tecnologia seno Del Estado Of Jalisco | Lugo-Cervantes E.,Research Center istencia En Tecnologia seno Del Estado Of Jalisco | Winterhalter P.,TU Braunschweig | Jerz G.,TU Braunschweig
Food Chemistry | Year: 2014

The polar constituents of peels from Citrus limetta variety Risso (Rutaceae) were investigated by a combination of two complementary chromatographic techniques consisting of preparative high-speed countercurrent chromatography (HSCCC), and off-line LC-ESI-MS/MS analysis to design a two-dimensional metabolite profile. Countercurrent chromatography (CCC) using solely immiscible solvent systems allowed the fractionation of principal components and an enrichment of minor concentrated metabolites from a crude polar solvent partition of C. limetta peels for subsequent structural identification by LC-ESI-MS/MS analysis. The combination of two very different chromatographic techniques resulted in lower detection limits for electrospray mass-spectrometry and revealed eighty-five compounds, including three abscisic acid derivatives, five limonoid glycosides, twenty-six dihydro-cinnamic and cinnamic acid glycosides, eleven flavanone glycosides, seven flavone glycosides, seventeen flavonol glycosides, including limocitrol and limocitrin derivatives. As a chemocharacteristic for C. limetta metabolites, many of the detected structures were linked to single and multiple 3-hydroxy-3-methyl-glutaryl (HMG) substitutions. C. limetta peels are a by-product of juice production, and not only the antioxidant fractions but also some of the fortified compounds could be used for food and pharmaceutical purposes. © 2014 Elsevier Ltd. All rights reserved.

Ramirez-Santiago C.,National Autonomous University of Mexico | Lobato-Calleros C.,National Autonomous University of Mexico | Espinosa-Andrews H.,Research Center istencia En Tecnologia seno Del Estado Of Jalisco | Vernon-Carter E.J.,Metropolitan Autonomous University
Dairy Science and Technology | Year: 2012

The structural-mechanical-sensory characteristics of cheese can be altered by reducing the fat content, but this can affect product acceptance by the consumer. A potential alternative for improving the characteristics of reduced-fat cheese (RFC) is to reinforce the protein network with biopolymers of complex coacervates which may act as building blocks within the continuous phase. The objective of this work was to determine the chemical composition, dynamic rheological properties, and overall sensory acceptability of four reduced-fat Petit-Suisse cheeses (RFC25, RFC50, RFC75, and RFC100) made by partial or total substitution (25%, 50%, 75%, and 100%) of milk fat contained in milk cream (MC) by whey protein isolate-lowmethoxyl pectin (WPI-LMP) complex coacervate (CC), in comparison to those of a full-fat Petit-Suisse cheese (FFC). The relative concentrations of the MC to CC determined the extent to which the buildup and rebodying occurred in the RFC cheeses, thus affecting their rheological properties and overall sensory acceptability. A linear regression model was determined which estimated the overall acceptability scores of the cheeses as a function of the changes in storage (G') and loss (G") moduli with storage time. RFC50 and RFC75cheeses displayed a mechanical-sensory response resembling that of the FFC cheese. © INRA and Springer-Verlag, France 2012.

Llamas-Gonzalez Y.Y.,Research Center istencia En Tecnologia seno Del Estado Of Jalisco | Flores-Valdez M.A.,Research Center istencia En Tecnologia seno Del Estado Of Jalisco
Revista de Investigacion Clinica | Year: 2013

Mycobacterial species have practically evolved along humankind, sometimes provoking serious diseases. Among them, tuberculosis (TB), produced by M. tuberculosis-complex bacteria, is historically the single most devastating infectious agent. Like many other microorganisms, M. tuberculosis resistant to antibiotics have risen as a consequence of selective pressure for mutants able to persist despite being attacked with drugs that would otherwise erradicate them from the infected person. Given the current long-term (6-9 months) therapy with multiple antibiotics, many people abandon their treatments, therefore promoting that bacteria that were not eliminated during therapy get exposed to suboptimal antibiotic concentrations, probably leading to mutations and drug resistance. In this scenario, extremely-drug resistant (XDR) TB was recognized not more than a decade ago, prompting concerns for a more complicated drug regimen with few available molecules. In recent years, either old antibiotics have been rediscovered as good measures to control XDR-TB, or new ones have emerged as alternatives to cure patients of this type of infection. In this work we aim to provide the medical community in Mexico with information of such drug regimens that have succesfully worked, in order to get their consideration for use in our country.

Nuno-Ayala A.,Unima Soluciones Naturales S.A. de C.V. Santo Tomas de Aquino 5715 | Rodriguez-Garay B.,Research Center istencia En Tecnologia seno Del Estado Of Jalisco | Gutierrez-Mora A.,Research Center istencia En Tecnologia seno Del Estado Of Jalisco
Plant Cell, Tissue and Organ Culture | Year: 2012

It is reported for the first time an efficient protocol for indirect somatic embryogenesis and plant regeneration for Jarilla heterophylla (Caricaceae). Initial explants were mature zygotic embryos obtained from seeds collected in two regions of the state of Jalisco, México (San Luis Soyatlán and Zacoalco de Torres). Two protocols for somatic embryogenesis used previously for Carica papaya were evaluated. The addition of 2,4-dichlorophenoxyacetic acid (2,4-D) to induction medium A1 without the addition of adenine hemisulphate yielded the best production of embryogenic callus with the higher total of somatic embryos per explant. With this protocol the plant regeneration process took about five months. © 2011 Springer Science+Business Media B.V.

Evangelista-Martinez Z.,Research Center istencia En Tecnologia seno Del Estado Of Jalisco
World Journal of Microbiology and Biotechnology | Year: 2014

The use of antagonist microorganisms against fungal plant pathogens is an attractive and ecologically alternative to the use of chemical pesticides. Streptomyces are beneficial soil bacteria and potential candidates for biocontrol agents. This study reports the isolation, characterization and antagonist activity of soil streptomycetes from the Los Petenes Biosphere Reserve, a Natural protected area in Campeche, Mexico. The results showed morphological, physiological and biochemical characterization of six actinomycetes and their inhibitory activity against Curvularia sp., Aspergillus niger, Helminthosporium sp. and Fusarium sp. One isolate, identified as Streptomyces sp. CACIS-1.16CA showed the potential to inhibit additional pathogens as Alternaria sp., Phytophthora capsici, Colletotrichum sp. and Rhizoctonia sp. with percentages ranging from 47 to 90 %. This study identified a streptomycete strain with a broad antagonist activity that could be used for biocontrol of plant pathogenic fungi. © 2013 Springer Science+Business Media Dordrecht.

Baigts Allende D.,Research Center istencia En Tecnologia seno Del Estado Of Jalisco
Vitae | Year: 2016

Concern for the preservation of natural resources to the food supply due to rapid population growth has led to the exploration of alternative food sources that meet the demands of society. The need for a sustainable diet that reduces the environmental impact but that does not compromise the quality of the food is led by emergent protein producing that play the role of animal proteins but which are highly available, efficient and low cost. The use of foliar biomass for the production of proteins is seen as a viable option for sustainable food production to supply the nutritional demands and to be used as techno-functional ingredients in the development of food products by studying the molecular structure of proteins at different length scales and their interaction in order to determine the final products characteristics. © 2016, Universidad de Antioquia. All rights reserved.

Flores-Valdez M.A.,Research Center istencia En Tecnologia seno Del Estado Of Jalisco
Frontiers in Microbiology | Year: 2016

Tuberculosis (TB) remains as a global public health problem. In recent years, experimental evidence suggesting the relevance of in vitro pellicle (a type of biofilm formed at the air-liquid interface) production as a phenotype mimicking aspects found by Mycobacterium tuberculosis-complex bacteria during in vivo infection has started to accumulate. There are still opportunities for better diagnostic tools, therapeutic molecules as well as new vaccine candidates to assist in TB control programs worldwide and particularly in less developed nations. Regarding vaccines, despite the availability of a live, attenuated strain (Mycobacterium bovis BCG) since almost a century ago, its variable efficacy and lack of protection against pulmonary and latent disease has prompted basic and applied research leading to preclinical and clinical evaluation of up to 15 new candidates. In this work, I present examples of vaccines based on whole cells grown as biofilms, or specific proteins expressed under such condition, and the effect they have shown in relevant animal models or directly in the natural host. I also discuss why it might be worthwhile to explore these approaches, for constructing and developing new vaccine candidates for testing their efficacy against TB. © 2016 Flores-Valdez.

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